Program of the 2001 International Worm Meeting - Sternberg Lab ...

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365 1. Culligan et.al. International Journal of Molecular Medicine (1998) 2:639-648. 2. Hutter et.al. Science (2000) 287:989-994 3. Bessou et.al. Neurogenetics (1998) 2:61-72 366. Use of C. elegans to study tumour suppressor genes Laurent Molin, Jean Pierre Magaud INSERM U453, Centre Leon Berard, 69373 Lyon Cedex 08 366 We are studying tumour suppressor genes in normal and neoplastic cells. These genes, whose function is altered in a large number of tumours, have a fundamental role in the cycle cycle, in cellular differentiation, cellular aging or in the response to genotoxic agents. We are using C. elegans in order to better understand the function of these genes and their position within complex signalling pathways. We will more particularly study homologues in C. elegans of the genes BTG-1, BTG-2, FRG-1, FVT-1 and of known interacting proteins. The C. elegans homologue of BTG-1 and BTG-2 is fog-3. Preliminary results will be presented and discussed.
367 367. Characterization of a C elegans homolog of a human tumor biology target Marsha Smith, Diane Levitan SPRI 2015 Galloping Hill, K15 C3600, Kenilworth, NJ 07033 We are investigating the role of a C. elegans homolog of a novel human gene indicated to be involved in tumor formation. No mutant strains are currently known to exist for this gene, so characterization has been done using RNAi and overexpression constructs. The RNAi phenotypes include slow maturation (twice as long as wildtype), some larval arrest/tiny adults, and a failure for the germ line to proliferate resulting in adult sterility. DAPI staining of the gonad shows that the germ line cells arrest in the mitotic prometaphase. Because of this gene’s involvement with cancer, the potential for a role in the ras pathway was examined. RNAi of this gene suppressed the multivulval phenotype in two let-60 gain of function mutants, let-60 (ga89) and let-60 (n1046), though exhibited no genetic interactions with let-23, sem-5, lin-45, mek-2, or lin-1. In all these genetic backgrounds the RNAi phenotypes of slow growth and lack of germ line proliferation were as strong as in N2s; therefore, the effect on ras activity appears to be indirect. We postulated one such indirect mechanism might be via an influence on cell cycle. To address this we analyzed the effect of RNAi in a cul-1 mutant background. cul-1 is required for cell cycle exit, and in a cul-1 mutant most postembryonic blast cells, including the germ line, overproliferate. RNAi of this gene in a cul-1 mutant did not effect the overproliferation of most blast cells. However the germ line showed the same extent of reduced proliferation as RNAi into wildtype animals. Therefore, it seems that wildtype cul-1 may act through this gene to regulate exit from the cell cycle in the germ line. Human mutations have been identified in this cancer gene. We have engineered similar mutation in the C. elegans gene and have made transgenic lines overexpressing either the wildtype or mutant forms. Evaluation of these transgenic lines and further analysis of this gene will be reported. 368. RNAi screen of tumorigenesis-related genes: potential developmental roles Aaron P. Putzke, Joel H. Rothman Interdepartmental Program in Biochemistry and Molecular Biology, UC Santa Barbara, Santa Barbara, CA 93106 We are performing an RNAi-based screen with C. elegans homologues of a variety of human tumor suppressors and oncogenes to characterize developmental processes that might illuminate mechanisms of tumorigenesis. We have observed an RNAi-induced phenotype for about half of the genes screened thus far. Two genes with particularly striking RNAi phenotypes are currently being characterized. The first gene encodes a cytoplasmic tyrosine kinase related to the human fer/fes oncogene. The predicted protein contains SH2 and tyrosine kinase domains, implying that it may interact with a receptor tyrosine kinase pathway. The RNAi-induced phenotype of this gene includes embryonic lethality with severe defects in morphogenesis, a markedly reduced rate of larval development, defects in germline differentiation, and clear (Clr) phenotypes. Collectively, these phenotypes suggest that this kinase acts in many events throughout development. A second gene encodes a protein with a RING-finger domain that is homologous to that of the human gene ret. This gene belongs to the RBCC gene family, one member of which, LIN-41, acts to regulate developmental timing in worms (Slack et al., 2000). RNAi of this gene causes some larvae to arrest at the L1/L2 stage and others to exhibit a delayed development into adulthood. In both cases, a blockage of the gut is seen as bacteria gradually accumulate within the gut lumen. A more in-depth characterization of each gene mentioned above will be presented focusing on expression patterns and possible cellular pathways in which the proteins may act. Slack, F.J., Basson, M., Liu, Z., Ambros, V., Horvitz, H.R., Ruvkun, G. (2000). Mol. Cell 5, 659-669. 368
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Program of the 2001 International W
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32. AN AXIN-LIKE PROTEIN FUNCTIONS
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68. Assembly and function of the sy
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106. Identifying muscle genes that
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143. Roles of unc-34 and unc-40 in
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177. Microevolution of vulval cell
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Gengyo-Ando, Yuji Kohara 214. Mutat
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248. Signaling pathways in heat acc
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283. UNC-16, A JNK signaling scaffo
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Immunity & infection Workshop Man-W
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356. pvl-5 prevents Pn.p cell death
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398. Isolation and analysis of chem
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441. Characterization of FERM-domai
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483. Reverse genetic analysis of a
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527. A second function of the APC-r
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567. A Screen for Mutations that En
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609. Multiple defects in a C. elega
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651. A Novel Ser/Thr Protein Kinase
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693. Direct interaction between IP3
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Einhard Schierenberg 735. Computer
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779. Evolution of homeotic gene fun
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823. Calcium Regulatory Sites in UN
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864. A screen for mutations affecti
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907. Light-increased reversal frequ
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949. Molecular and Genetic Characte
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992. Forward and Reverse Genetic Ap
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1034. Pristionchus pacificus mutant
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1077. Expression of a C. elegans Mu
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1 1. MSP Signaling: Beyond the Sper
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3 3. IN VIVO DYNAMICS OF POP-1 ASYM
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4 PHA-4 is able to bind these sites
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6 identical to a mammalian nuclear
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8 spacing between the polyA site of
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11 11. 7,500 Genes and the Transiti
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13 13. Caenorhabditis Genetics Cent
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15 15. WormBase: A Web-accessible d
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17 17. ego-1, a link between germli
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19 19. Potential for Cross-Interfer
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22 basal transcription and further
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25 25. Two RGS proteins that inhibi
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28 28. Novel downstream targets of
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30 30. Modulation of inductive sign
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33 33. A genetic dissection of the
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35 35. The Profilin PFN-1 is requir
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37 37. spn-4 encodes a putative RNA
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39 39. Phosphotyrosine signaling ac
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41 lengthened interphase and M phas
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43 DSH-2 asymmetrically. In mom-5 m
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46 46. Analysis of kin-13 PKC and d
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48 48. SLO-1 Potassium Channel Regu
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51 51. unc-122 and unc-75: Two gene
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53 53. sol-1 encodes a novel protei
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55 cells. These findings suggest th
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57 addition, as with the FLP cells,
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60 60. Regulation of olfactory rece
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62 62. osm-5, the C. elegans homolo
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64 64. Multi-pathway Regulation of
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65 inactivation, in fact, affects p
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67 recombination. Altogether, this
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70 70. MES-4, a protein required fo
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72 72. Characterization of the C. e
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74 74. The intracellular domain of
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76 76. Regulation of fem-3 mRNA by
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78 78. Hermaphrodite or female? Spe
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80 80. Haldane’s Rule in Caenorha
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82 82. A cyclic GMP-dependent prote
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84 84. IDENTIFICATION OF AMP KINASE
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86 of mutant genomic DNA showed tha
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88 predauer. When this gene was kno
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90 However, when they are cultured
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92 sax-1 encodes a serine/threonine
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94 1 Dent et al. (1997) EMBO J. 16:
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96 ttx-1 encodes a homeodomain prot
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98 proteins is unknown. Using GFP f
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100 (Miyahara et al. 1999 Internati
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103 103. In Vivo Optical Imaging of
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105 105. Creating transgenic lines
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107 107. M5 controls terminal bulb
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108 Similarly, PBO-4 contains a pot
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110 unknown signal or signals from
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112 acid repeats (1). These data su
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114 function to limit the rate of a
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116 other neuron tested, restores s
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119 119. UNC-58 IS AN UNUSUAL POTAS
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121 121. Analysis of the Sex-Specif
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123 123. Dissection of DNA damage c
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125 125. CED-12, A COMPONENT OF A R
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127 127. A Novel Pharmacogenetic Mo
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129 predicted WW domain protein (ZK
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132 132. zag-1, a Zn finger-homeodo
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134 134. A possible repulsive role
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136 136. Genetic Analysis of the Ka
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138 138. Interacting proteins with
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140 140. A genetic network involvin
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142 142. A Constitutively Active UN
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144 144. Putative matrix proteins w
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146 146. Maturation of the C. elega
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148 We have also initiated an analy
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151 151. zyg-12 is required for the
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153 153. The mitosis-regulating kin
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155 155. CDC-42, AGS-3 and heterotr
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157 157. The NED-8 ubiquitin-like c
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159 159. OOC-5, required for polari
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161 3) Loss of dlg-1 activity leads
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163 epithelial tightness. Consisten
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165 ITR-1 regulates cytoskeletal re
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167 i) male tail defects; in genera
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170 170. mua genes function within
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172 lin-44(n1792); tlp-1(mh17) doub
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174 required for the generation of
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176 repressing the fusogenic activi
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178 lin-15A may therefore function
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181 181. Two enhancers of raf, eor-
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183 In order to elucidate the mecha
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185 We have generated transgenic an
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187 expression reporter construct d
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189 for mRNA transcription, and may
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192 192. From Binding Sites to Targ
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194 194. The novel factor PEB-1 fun
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196 196. Functional analysis of spl
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198 198. Operon Resolution, Usage a
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200 or CTP). Third, incorporation r
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202 late-stage populations that LIN
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204 Mutations in ocr-2 recapitulate
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206 specific promoters to drive the
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208 sensory signaling, we behaviora
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210 important for food-temperature
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213 213. Translational control of m
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215 215. MEX-3 Interacting Proteins
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217 217. The role of PLP-1 in mesen
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219 219. 4D-analysis of a collectio
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221 We also analyzed sperm developm
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224 224. lin-9, lin-35 and lin-36 n
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226 226. CUL-4 functions to prevent
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228 228. Genes that ensure genome s
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230 230. Open-reading-frame sequenc
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232 232. A snip-SNP map of the C. e
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234 WormPD, as well as the other vo
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236 References: 1. Srinivasan. J et
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239 239. Rates and Patterns of Muta
Page 281 and 282:
242 242. Global Analysis of Gene Ex
Page 283 and 284:
244 244. Signals from the reproduct
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247 247. hif-1, a homolog of mammal
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250 250. The splicesomal Sm protein
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252 252. GLHs associate with a LIM
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254 254. Control of germline cell f
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256 region where GLD-1 levels fall
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258 ClC-2 and CLH-3 share 40% amino
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261 experiments showed that the two
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263 located within the ATPase domai
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265 We are using the collection of
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267 in C. elegans. 268. Mutations i
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269 but they are often misplaced. F
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271 M-lineage. By ablation it was s
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273 aph-2 and Nicastrin are partial
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275 hlh-2(RNAi) experiments suggest
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278 278. Mesodermal cell fate speci
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280 encoding a Rho GTPase. Overexpr
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282 To test the lipid-binding model
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284 carboxy-terminal EH domain. Yea
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287 287. FUSOMORPHOGENESIS: A TALE
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289 289. Chromosome-Wide Regulation
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290 substrate(s) is currently under
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292 more cycles of apparent replica
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294 ags-3.2 and ags-3.3 encode esse
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297 297. Mutations in the C elegans
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299 299. THE ACTIN-BINDING PROTEIN
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301 301. CED-12 functions in the CE
Page 337 and 338:
303 303. The C. elegans Autosomal D
Page 339 and 340: 305 To determine if cdf-1 affects t
Page 341 and 342: 307 part from a target dependent me
Page 343 and 344: 309 References: 1. Hekimi S, Boutis
Page 345 and 346: 311 ligand may be a sterol derivati
Page 347 and 348: 314 Mutations in two loci, daf-12 a
Page 349 and 350: 316 which show a strong neuronal bi
Page 351 and 352: 318 318. Evolution of germ-line sig
Page 353 and 354: 320 320. Identifying universal Serr
Page 355 and 356: 322 wild-type cell lineage, which d
Page 357 and 358: 324 quality of many commercial anti
Page 359 and 360: 326 While the nervous system appear
Page 361 and 362: 330 330. Molecular analysis of agin
Page 363 and 364: 333 333. Identification of two nove
Page 365 and 366: 335 and prolonged life span. Unlike
Page 367 and 368: 338 338. Caloric restriction activa
Page 369 and 370: 340 full-length receptor. Its abund
Page 371 and 372: 343 343. A bin’s-worth of bounty:
Page 373 and 374: 345 345. Pheromone Regulation of da
Page 375 and 376: 347 longest pure poly-Q run is 8 am
Page 377 and 378: 350 daf-7(e1372)animals (63%, n=154
Page 379 and 380: 352 In addition to their effect on
Page 381 and 382: 354 transcription or through a diff
Page 383 and 384: 356 wild-type animals. Curiously, c
Page 385 and 386: 359 359. abl-1 Regulates C. elegans
Page 387 and 388: 362 362. What factors regulate prog
Page 389: 364 expression is confined to neuro
Page 393 and 394: 370 370. MOLECULAR REGULATORS OF PO
Page 395 and 396: 372 using unc-51 promoter for panne
Page 397 and 398: 374 Taken together, these experimen
Page 399 and 400: 377 377. ACM-2, a neuronal muscarin
Page 401 and 402: 379 strain. I intend to investigate
Page 403 and 404: 382 382. Isolation and analysis of
Page 405 and 406: 384 neurons but not in the muscles
Page 407 and 408: 386 neurons is 25 ± 4 μm. mec-7::
Page 409 and 410: 389 389. Engineering pharyngeal pum
Page 411 and 412: 391 391. ELECTROPHYSIOLOGICAL ANALY
Page 413 and 414: 394 394. Mechanisms involved in reg
Page 415 and 416: 396 396. Sniffing out the mechanism
Page 417 and 418: 398 398. Isolation and analysis of
Page 419 and 420: 401 401. Identification and Charact
Page 421 and 422: 403 403. The application of novel p
Page 423 and 424: 406 406. Activity-dependent transcr
Page 425 and 426: 408 expression of rcn-1 through GFP
Page 427 and 428: 411 411. Isolating redundant pathwa
Page 429 and 430: 413 413. syd-2 and rpm-1 function s
Page 431 and 432: 415 415. A screen to identify regul
Page 433 and 434: 417 bodies. Therefore, at least one
Page 435 and 436: 420 two predictions: (1) that if it
Page 437 and 438: 423 examined expression patterns of
Page 439 and 440: 425 gcy-5, gcy-6, and gcy-7. In eac
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427 427. Isolation of mutants defec
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429 429. Two-hybrid Screen for UNC-
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431 phenotypes of both mutants are
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433 specifically involved in retrog
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435 fluorescence did not cause the
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437 associate with endogenous worm
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440 440. Microtubule based conventi
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442 constructed double mutants of v
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444 connections between the canal,
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446 microtubules. (This work will a
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449 449. or358ts is involved in mit
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452 452. CeMCAK, a C. elegans kines
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455 455. Characterization of rot-2,
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457 followed by injection into wild
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459 while astral microtubules are u
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463 463. Investigating the possibe
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465 egl-35 mutations synthetically
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467 associated with cyb (gk35). 468
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469 cytokinesis. Cytokinesis can be
Page 479 and 480:
472 472. daz-1 , a C. elegans homol
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474 474. him-8 and him-5 Philip M.
Page 483 and 484:
477 477. Identifying new genes that
Page 485 and 486:
479 against the lamprey vitellogeni
Page 487 and 488:
481 nM calyculin A completely inhib
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483 F30A10.10 mutant showed vacuolo
Page 491 and 492:
486 486. spe-39 and Orthologs: A Ne
Page 493 and 494:
488 mediated degradation of specifi
Page 495 and 496:
491 491. Screening for sperm compet
Page 497 and 498:
494 494. A possible role for gcy-31
Page 499 and 500:
496 496. Genes Mediating Elongation
Page 501 and 502:
498 498. A Role for VAV-1 in Pharyn
Page 503 and 504:
500 We have narrowed the chromosoma
Page 505 and 506:
502 polarized secretion, activation
Page 507 and 508:
505 a low-penetrance "gob" phenotyp
Page 509 and 510:
508 508. Role of PDZ domain protein
Page 511 and 512:
511 511. Identification of discs la
Page 513 and 514:
514 alternatively spliced region of
Page 515 and 516:
517 517. Screening for Dorsal Inter
Page 517 and 518:
519 519. A Genetic Screen for New M
Page 519 and 520:
522 522. The C.elegans Mi-2 chromat
Page 521 and 522:
525 LIN-39 phosphorylation and dock
Page 523 and 524:
528 528. Suppression of mutation-in
Page 525 and 526:
530 approach should allow us to scr
Page 527 and 528:
533 533. MAB-18 BI-DIRECTIONALLY RE
Page 529 and 530:
535 535. Regulation of the ray posi
Page 531 and 532:
537 ankyrin and unc-34 are being te
Page 533 and 534:
540 540. Characterization of nuclea
Page 535 and 536:
542 lis-1 in C. elegans. 543. C. el
Page 537 and 538:
546 546. Essential roles for C. ele
Page 539 and 540:
549 549. The cDNA sequence and expr
Page 541 and 542:
551 551. A Caenorhabditis elegans c
Page 543 and 544:
554 554. Activation of protein degr
Page 545 and 546:
556 556. Identification and charact
Page 547 and 548:
558 558. Expression of C. elegans A
Page 549 and 550:
560 560. Functional analysis of pot
Page 551 and 552:
562 1. Kent, W.J. and Zahler, A.M.
Page 553 and 554:
565 565. Increased sensitivity to R
Page 555 and 556:
568 568. Pulling the Trigger of Tra
Page 557 and 558:
571 was about 100bp. We are current
Page 559 and 560:
574 574. Tissue expression, interac
Page 561 and 562:
576 expression at other sites. Stud
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579 579. Nuclear Receptors Required
Page 565 and 566:
582 582. Using Genomics to Study Ho
Page 567 and 568:
584 584. Morphological Evolution of
Page 569 and 570:
586 Literature cited: Denver, D.R.,
Page 571 and 572:
590 590. There are at least four eE
Page 573 and 574:
592 dominance, independent assortme
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594 The class meets once/week for 4
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597 597. Surfing the Genome: Using
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600 presented at this meeting by Pr
Page 581 and 582:
603 603. Caenorhabditis elegans as
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605 605. A bummed pathogen meets wi
Page 585 and 586:
607 607. Developing genetic methods
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609 phenotype suggesting that the r
Page 589 and 590:
612 612. Insulin/IGF-like peptides
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614 dauer morphogenesis, such as th
Page 593 and 594:
617 617. An Adaptive Response Exten
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620 620. Role of DAF-9 CYP450 in th
Page 597 and 598:
622 622. Nuclear Lamina Characteriz
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Page 601 and 602:
627 627. Phagocytosis of necrotic c
Page 603 and 604:
629 and clearance in C.elegans. 1.F
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631 mediated endocytosis (Grant and
Page 607 and 608:
634 634. BAG1, AN HSP70 CO-CHAPERON
Page 609 and 610:
637 637. HSP90 mRNA in the nematode
Page 611 and 612:
640 640. A study of the osmotic str
Page 613 and 614:
642 To develop a genetic method for
Page 615 and 616:
644 Mammalian FGFRs activate RAS si
Page 617 and 618:
647 647. Genetic analysis of the Ra
Page 619 and 620:
649 mechanism of regulation of RGS
Page 621 and 622:
651 cuticular structures ("treads")
Page 623 and 624:
654 654. Characterization of the sp
Page 625 and 626:
656 Serotonin-deficient mutants and
Page 627 and 628:
659 659. Imaging of neuroal activit
Page 629 and 630:
661 661. Making the Matrix: MEC-1,
Page 631 and 632:
663 663. Elaborating the compositio
Page 633 and 634:
664 to at least the ~100 cell stage
Page 635 and 636:
666 The alpha-amino-3-hydroxy-5-met
Page 637 and 638:
669 669. GENES SHOWING ALTERED EXPR
Page 639 and 640:
671 671. EVIDENCE FOR A CHA-1-SPECI
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673 these mutants further supportin
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675 1 Horvitz HR et al, Science 216
Page 645 and 646:
679 679. Specificity of G-protein s
Page 647 and 648:
681 681. A Genetic Interaction Betw
Page 649 and 650:
683 683. A Dopamine Receptor in C.
Page 651 and 652:
685 685. Characterizing the C. eleg
Page 653 and 654:
688 688. Alternative isoforms and m
Page 655 and 656:
690 contortus subunits. 691. Expres
Page 657 and 658:
694 694. The unc-63 gene encodes a
Page 659 and 660:
696 696. Identifying Modulators of
Page 661 and 662:
699 699. CARGO RECOGNITION BY SYNAP
Page 663 and 664:
701 701. Cloning and characterizati
Page 665 and 666:
703 703. zd8 causes cell migration
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705 homeodomain and thus deletes th
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707 target(s). We have seen no noti
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710 710. THE IDENTIFICATION OF PROT
Page 673 and 674:
711 offers a simple system to study
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713 autonomous. Since the MIG-17 me
Page 677 and 678:
716 716. ADM-1 AND ADM-2: DISINTEGR
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718 718. A SCREEN FOR REGULATORS OF
Page 681 and 682:
720 720. An in vitro System for Stu
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723 723. Localization of innexins T
Page 685 and 686:
725 725. Characterization of C. ele
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727 no mutant phenotypes were obser
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729 the hyodermis and pharynx remai
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732 α-tubulin was normal. These ob
Page 693 and 694:
735 735. Computer Simulation of Ear
Page 695 and 696:
738 738. Toward an understanding of
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740 We were intrigued to find that
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742 alleles fail to complement evl-
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744 each nucleus seemed equal and n
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747 747. STU-4, the C. elegans homo
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749 preparation and 2D-gel electrop
Page 707 and 708:
752 752. SNP Mapping of ego-3, a C.
Page 709 and 710:
755 755. Functional analysis of two
Page 711 and 712:
757 757. The C. elegans ptc and ptr
Page 713 and 714:
759 759. PGL-1, PGL-2, and PGL-3, a
Page 715 and 716:
761 761. The nuclear receptor SEX-1
Page 717 and 718:
764 764. TRA-1 is a Phosphoprotein
Page 719 and 720:
767 767. C. elegans SMC complexes:
Page 721 and 722:
770 770. Characterizing the C. eleg
Page 723 and 724:
772 in skn-1(zu67) homozygotes gcs-
Page 725 and 726:
774 ceh-23 and kal-1, requires ceh-
Page 727 and 728:
778 778. What is TLF doing during t
Page 729 and 730:
781 781. Characterization of tissue
Page 731 and 732:
783 somatic gonad development. The
Page 733 and 734:
786 786. Functional requirement for
Page 735 and 736:
789 789. IDENTIFICATION AND CHARACT
Page 737 and 738:
792 792. Characterization of a New
Page 739 and 740:
795 795. Analysis of lin-1 Mutants
Page 741 and 742:
798 798. spr GENES: SUPPRESSORS OF
Page 743 and 744:
801 801. Genetics and genomics of h
Page 745 and 746:
803 803. Control of ins-33 transcri
Page 747 and 748:
805 We are currently determining wh
Page 749 and 750:
807 We are studying the ability of
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809 mutants isolated in our let-7 p
Page 753 and 754:
811 homologue in C. elegans, tim-1,
Page 755 and 756:
814 814. Characterization of the mu
Page 757 and 758:
816 816. sem-3 encodes a cis-prenyl
Page 759 and 760:
818 818. PARAMYOSIN-GFPs RESCUE MUT
Page 761 and 762:
821 821. Effect of the alpha 2 Ca 2
Page 763 and 764:
823 823. Calcium Regulatory Sites i
Page 765 and 766:
825 825. CAM-1/KIN-8 Receptor Tyros
Page 767 and 768:
828 828. Loss of a dynamin related
Page 769 and 770:
830 830. Nematodes with Mitochondri
Page 771 and 772:
833 833. The Effects of Electron Tr
Page 773 and 774:
835 835. Cryofixation of C. elegans
Page 775 and 776:
837 837. Electron Tomography at an
Page 777 and 778:
840 840. A High Resolution Digital
Page 779 and 780:
842 842. New! Improved! Fixation pr
Page 781 and 782:
845 845. Correlation between transc
Page 783 and 784:
847 chromatin-IP-microarray strateg
Page 785 and 786:
850 850. NEMATODE.NET, a Tool for N
Page 787 and 788:
854 854. Characterization of a Shor
Page 789 and 790:
858 858. A genetic screen to identi
Page 791 and 792:
860 progress to identify these neur
Page 793 and 794:
863 genomes, and a 25 0 C F 2 Daf-c
Page 795 and 796:
866 866. The role of egl-32 in egg
Page 797 and 798:
868 868. Defining new components of
Page 799 and 800:
871 871. sma-9 and TGFbeta Signalin
Page 801 and 802:
874 acy-2 was previously reported t
Page 803 and 804:
877 877. Identification of lin-44(s
Page 805 and 806:
879 alpha-synuclein deposits in the
Page 807 and 808:
881 constructs encoding the extrace
Page 809 and 810:
884 884. Exploring necrotic cell de
Page 811 and 812:
886 886. SNAP-25, a Rat General Ane
Page 813 and 814:
888 888. Gain-of-function mutations
Page 815 and 816:
891 891. mab-21 expression is regul
Page 817 and 818:
894 894. Reproductive and Acute Tox
Page 819 and 820:
896 effects of chronic fluoxetine i
Page 821 and 822:
898 and binds NADP. Two such domain
Page 823 and 824:
900 PA phosphatase (PAP). Thus, tho
Page 825 and 826:
903 903. Characterization of the th
Page 827 and 828:
905 hours (0.5 ~ 4 hr), which impli
Page 829 and 830:
908 908. Factors that affect the su
Page 831 and 832:
911 911. A conserved mechanism of s
Page 833 and 834:
914 914. A screen for dominant enha
Page 835 and 836:
916 916. Are input and output defec
Page 837 and 838:
918 918. Visualizing synapses in th
Page 839 and 840:
920 920. The Role of Rapsyn in C el
Page 841 and 842:
922 922. Genetic and Phenotypic Ana
Page 843 and 844:
924 924. Probing the Go-Gq Signalin
Page 845 and 846:
926 PQR). These neurons are all con
Page 847 and 848:
928 Localized expression of an UNC-
Page 849 and 850:
930 rac-2 are each necessary for di
Page 851 and 852:
933 933. Molecular characterization
Page 853 and 854:
935 Hrs/Hrs-2, whereas the open rea
Page 855 and 856:
938 938. The C. elegans homologue o
Page 857 and 858:
941 941. unc-20 MAPS TO A REGION TH
Page 859 and 860:
943 943. Mutations in let-767, a st
Page 861 and 862:
946 946. Role of sphingolipids in C
Page 863 and 864:
948 948. Characterisation of mammal
Page 865 and 866:
950 950. ISOLATION AND CHARACTERIZA
Page 867 and 868:
952 Xenopus oocytes. We have demons
Page 869 and 870:
954 described gene involved in the
Page 871 and 872:
957 in the translational repression
Page 873 and 874:
960 posterior daughter E and this s
Page 875 and 876:
962 not eliminated the extra hypode
Page 877 and 878:
964 out of species homologue. Lastl
Page 879 and 880:
967 967. Chromosome remodeling duri
Page 881 and 882:
970 970. The HMGA proteins of C. el
Page 883 and 884:
973 973. In vivo studies of the nuc
Page 885 and 886:
975 1. Hecht et al. (1987) J. Cell
Page 887 and 888:
977 cul-2 embryonic phenotypes. The
Page 889 and 890:
979 1.Uhlman, F., Wernic, D., Poupa
Page 891 and 892:
981 similar to the tim-1 (RNAi) phe
Page 893 and 894:
983 Here we report our progress on
Page 895 and 896:
985 this mutation mainly affects th
Page 897 and 898:
987 the identity and fate of the tw
Page 899 and 900:
989 nos-2 RNA is not translated eff
Page 901 and 902:
991 diplotene earlier than in wild
Page 903 and 904:
993 Three of the Pro mutants we ide
Page 905 and 906:
Page 907 and 908:
998 meiotic/pachytene region of tra
Page 909 and 910:
1001 therefore the RNAi screen of a
Page 911 and 912:
1004 1004. Is PAR-2 an E3 Ubiquitin
Page 913 and 914:
1007 P12 specification phenotype of
Page 915 and 916:
1010 1010. A screen for factors aff
Page 917 and 918:
1012 reference to sensory ray 6 dif
Page 919 and 920:
1015 1015. Defining Regulatory Regi
Page 921 and 922:
1017 1017. The Roles of cis-element
Page 923 and 924:
1019 1019. Computer prediction of c
Page 925 and 926:
1022 to binding sites for known C.
Page 927 and 928:
1024 (1) Berset et al. Science 291,
Page 929 and 930:
1027 1027. FUNCTIONAL STUDIES OF TH
Page 931 and 932:
1029 1029. Organogenesis of the C.
Page 933 and 934:
1031 these pseudovulvae are connect
Page 935 and 936:
1033 between induction and division
Page 937 and 938:
1036 mab-5 mutants. [1] Sommer R.J.
Page 939 and 940:
1039 1039. Cell fate specification
Page 941 and 942:
1042 regulated either directly or i
Page 943 and 944:
1045 1045. GENETIC INTERACTIONS BET
Page 945 and 946:
1047 1047. Prolyl 4-hydroxylase - e
Page 947 and 948:
1050 1050. Chemical and Genetic Inh
Page 949 and 950:
1053 lysate. Recently, it was sugge
Page 951 and 952:
1055 1055. Mutants with larger body
Page 953 and 954:
1057 To further understand which ge
Page 955 and 956:
1061 1061. ceh-13 shares overlappin
Page 957 and 958:
1063 1063. Novel and Atypical Recep
Page 959 and 960:
1065 1065. Comparative study of lin
Page 961 and 962:
1067 1067. Molecular Analysis of da
Page 963 and 964:
1069 immune system of the human hos
Page 965 and 966:
1073 1073. The regulation of gut de
Page 967 and 968:
1076 function of the nhr-85 gene. W
Page 969 and 970:
1078 propagation involves feedback
Page 971 and 972:
1080 by the lack of del-1::GFP expr
Page 973 and 974:
1082 Mapping and rescue experiments
Page 975 and 976:
1085 1085. A new method of analyzin
Page 977 and 978:
1088 1088. A SURVEY OF CHEMICALS AF
Page 979 and 980:
1090 618-codon open reading frame.
Page 981 and 982:
1093 1093. Toward a Genome-Wide RNA
Page 983 and 984:
1095 primers, formation of deletion
Page 985 and 986:
1098 1098. Thermokinesis: A New Ass
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