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Life_Science_2023_NZYTech_MKT-080

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RNA & cDNA<br />

RNA SYNTHESIS<br />

<strong>NZYTech</strong> offers convenient kits for the in vitro transcription of DNA into RNA using a T7 RNA polymerase that is highly specific<br />

for T7 phage promoters. The enzyme is also provided separately for your routine protocols.<br />

NZY T7 RNA Synthesis kit<br />

MB35301<br />

50 reactions<br />

NZY T7 High Yield RNA Synthesis kit<br />

MB36301<br />

50 reactions<br />

T7 RNA polymerase<br />

MB<strong>080</strong>01 10000 U (20 U/µL)<br />

MB<strong>080</strong>03 10000 U (200 U/µL)<br />

cDNA SYNTHESIS<br />

Reverse transcriptases<br />

NZY M-MuLV and NZY Reverse Transcriptases lack 3’-5’ exonuclease activity and have no intrinsic RNase H activity. NZY Reverse<br />

Transcriptase is a thermostable (50-55 O C) and sensitive enzyme with fast synthesis capacity (30 min reaction). Supreme NZYReverse<br />

Transcriptase was recently developed to increase inhibitor resistance, range and thermostability.<br />

NZY M-MuLV Reverse Transcriptase<br />

MB08301 20000 U<br />

MB08302 100000 U<br />

NZY Reverse Transcriptase<br />

MB12401 20000 U<br />

MB12402 100000 U<br />

Lyo NZY Reverse Transcriptase<br />

MB40901<br />

100000 U<br />

NZYReverse Transcriptase vs Competitor T<br />

NZYReverse Transcriptase<br />

Slope: -3.298; Eff%: 101.03<br />

<strong>NZYTech</strong> <strong>2023</strong><br />

Competitor T<br />

Slope: -3.346; Eff%: 99.02<br />

NZYReverse Transcriptase vs Competition. Two-step RT-qPCR to detect the GAPDH gene from mouse total RNA (dilutions from 1 μg to 0.1 ng). After reverse transcription, the cDNA<br />

obtained was used in a qPCR assay with NZYSupreme qPCR Green Master Mix (2x) (Cat. No. MB419).<br />

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