Life_Science_2023_NZYTech_MKT-080

PRINCIPLES & FEATURES
UV Tests
NZYTech test kits are based on enzymatic reactions and performed using spectrophotometric methods.
The principle of the enzymatic tests is based on the NAD(P) + /NAD(P)H system and uses highly pure enzymes engineered
to display a premium performance. The enzymes used in these analytical kits produce or consume NAD(P)H, which strongly
absorbs the UV radiation at 340 nm (extinction coefficient of 6300 M -1 cm -1 ).
Absorbance at
340 nm
NADH
NADPH
NADH
NADPH
Analyte
ENZYME
Product
NAD +
NAD +
NADP + NADP +
Reaction Time
Colorimetric tests
The principle of the enzymatic tests based on a chromogenic reaction is the formation of a coloured compound, which absorbs
at the visible region of the spectrum. The coloured compound results from the interaction between the product of a
first enzymatic reaction and a chromogenic compound. In this case, the concentration of the analyte must be determined by
using a standard curve.
• Endpoint analysis
• Easy to use, simple protocols
• Rapid analysis
• Accuracy and precision
• Safe to the operator
• Standards included
!
Required material
NZYTech 2023
• Spectrophotometer
• Micropipettes set with disposable plastic tips to accurately dispense volumes from 20 μL to 1000 μL
• Cuvettes
• Basic filtering or other simple sample treatment device
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