CELL BIOLOGY OF THE NEURON Polarity ... - Tavernarakis Lab

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Cell Biology of the Neuron: <strong>Polarity</strong>, Plasticity and Regeneration, Crete 2011 Axon Extension Occurs Independently of Centrosomal Microtubule Nucleation Michael Stiess 1 , Nicola Maghelli 2 , Lukas Kapitein 3 , Susana Gomis-Rüth 1 , Michaela Wilsch-Bräuninger 2 , Casper Hoogenraad 3 , Iva Tolic-Norrelykke 2 , Frank Bradke 1 1 Max Planck Institute of Neurobiology, Martinsried 2 Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 3 Erasmus Medical Center, Department of Neuroscience, Rotterdam The centrosome is the classical site of microtubule nucleation and is thought to be essential for axon growth and neuronal differentiation; processes that require microtubule assembly. Here, we found that the centrosome loses its function as a microtubule organizing center (MTOC) during neuronal development. Axons still extended and regenerated through acentrosomal microtubule nucleation and axons continued to grow after laser ablation of the centrosome in early neuronal development. Thus, decentralized microtubule assembly enables axon extension and regeneration and, after axon initiation, acentrosomal microtubule nucleation arranges the cytoskeleton, which is the source of the sophisticated morphology of neurons. Presented by: Stiess, Michael Poster No 108 Blue Session 190
Cell Biology of the Neuron: <strong>Polarity</strong>, Plasticity and Regeneration, Crete 2011 Cyclin-Dependent Kinase 5 Regulates Calcium Influx through N-type Calcium Channels Susan Su, Jennifer Pan, Benjamin Samuels, Khaing Win, Susan Zhang, David Yue, Li-Huei Tsai Cyclin-dependent kinase 5 (Cdk5) is a proline-directed serine/threonine kinase expressed in postmitotic neurons of the central nervous system and requires p35, its binding partner, for activity. Besides its well-known role in establishing the cytoarchitecture of the developing brain and its implication in a number of neurodegenerative diseases such as Alzheimer’s disease, amyotrophic lateral sclerosis (ALS), Neiman Pick’s Type C disease, and ischemic brain injury, recent evidence suggest that Cdk5 plays a key role in modulating synaptic functions. A growing list of characterized synaptic substrates include amphiphysin, dynamin, ephexin1, Munc-18, synapsin, and PSD-95, proteins that are involved in various functions such as vesicle trafficking, synapse formation and function or synaptic transmission. We previously showed that Cdk5 regulates synaptogenesis through phosphorylation of the presynaptic scaffolding molecule CASK. Upon phosphorylation by Cdk5, CASK dissociates from liprin-α, localizes to the synaptic compartment and associates with several presynaptic scaffolding proteins such as Mint1, Veli, and the α1B pore-forming subunit of the N-type voltage-gated calcium channels (CaV2.2), where it promotes calcium influx through CaV2.2 at presynaptic terminals. We now demonstrate that Cdk5 interacts with and phosphorylates CaV2.2. Furthermore, from whole-cell patch clamp recordings in heterologous cells we observe that Cdk5/p35 causes a twofold increase in calcium influx through CaV2.2. Our results can provide novel insights into how Cdk5 modulates CaV2.2 to regulate synaptic function. Understanding Cdk5 signaling could lead to the development of new therapeutics targeting Cdk5 or CaV2.2 for neuropathic pain or neurodegenerative conditions. Presented by: Su, Susan 191 Poster No 109 Red Session
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The meeting was funded in part by E
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These abstracts should not be cited
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CYFIP1, a Neuronal eIF4E‐BP, Link
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POSTER ABSTRACTS Poster # [‐R(ed)
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030‐B Paracrine Pax6 Activity Reg
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058‐R Dynein Light Chain LC8 is a
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088‐R Spectraplakins ‐ Cytoskel
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121‐R Calmyrin1 Binds to SCG10 Pr
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18.00 ‐ 18.15 Non Hyperpolarizing
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10.00 ‐ 10.30 Neurotrophin Regula
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SESSION 10 SYNAPTOGENESIS Chairpers
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da F. Costa, L. 119 D'Adamo, P. 108
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Lewallen, K. 145 Li, Y.‐H. 201 Li
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Teuling, E. 141 Thiebes, K. 87 Thom
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Cell Biology of the Neuron: Polarit
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