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<strong>STLCC</strong> Center for Plant and Life Sciences; <strong>BRDG</strong> <strong>Park</strong><br />
Molecular Biology Lab<br />
Instrumentation Room<br />
Faculty Office Classroom and Conf Rm<br />
<strong>STLCC</strong>: Morrison 8/20/2012<br />
Detect Pictures to see Room Layouts<br />
Major Equipment, Instrumentation<br />
and other Photos<br />
Prep and Equipment Rooms<br />
Prepared by : Bob Morrison, Instrumentation Specialist, Bmorrison@stlcc.<strong>edu</strong><br />
Tissue Culture Lab<br />
Bioprocessing Lab<br />
Greenhouse<br />
Space at Danforth<br />
1
<strong>BRDG</strong> <strong>Park</strong> : CPLS Entrance From North <strong>Park</strong>ing Area<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 2
<strong>BRDG</strong> <strong>Park</strong>: CPLS General Layout, Approx 10,500 sqft<br />
Molecular<br />
Lab<br />
Instrumentation<br />
Room<br />
Conference<br />
Prep Rooms<br />
Autoclave<br />
Freezers, Ice<br />
Eye Wash<br />
Shower<br />
Classroom<br />
Tissue<br />
Culture Lab<br />
Faculty<br />
Break Room<br />
Mechanical Entrance<br />
Office/Staff<br />
Eye Wash<br />
Shower<br />
Bioprocessing<br />
Lab<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 3
<strong>BRDG</strong> <strong>Park</strong> : CPLS Main Entrance<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 4
<strong>BRDG</strong> <strong>Park</strong> : CPLS Entrance Hall<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 5
<strong>STLCC</strong> Center for Plant and Life Sciences (CPLS) Office<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 6
BRD <strong>Park</strong>: CPLS Office Reception Area<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 7
<strong>BRDG</strong>: CPLS Informal Conference Area at Entrance<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 8
<strong>BRDG</strong>: CPLS Break/Refreshment Area<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 9
<strong>BRDG</strong>: CPLS Break Room, Vending<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 10
<strong>BRDG</strong>: CPLS Faculty/Staff Office, R123<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 11
Room#<br />
Eye Wash<br />
Shower<br />
126<br />
126A<br />
E<br />
Y<br />
E<br />
<strong>BRDG</strong>-CPLS: Tissue Culture Lab, R124, Layout<br />
126C<br />
Cold<br />
Room<br />
B<br />
I<br />
O<br />
C<br />
A<br />
B<br />
B<br />
I<br />
O<br />
C<br />
A<br />
B<br />
S<br />
C<br />
O<br />
P<br />
E<br />
124A<br />
Electro<br />
Pulsers<br />
125<br />
CRO<br />
Office<br />
Instr<br />
Scope, PC<br />
Doc<br />
Camera<br />
Smart<br />
Cyclers x 4<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 12<br />
124<br />
Spectro<br />
Room #123<br />
Faculty Office<br />
PCR<br />
Dual<br />
Plate<br />
Washer<br />
Water<br />
Bath x 2<br />
Liquid<br />
Handling<br />
BioMek<br />
Robotic<br />
Plant<br />
Inc<br />
CO2<br />
Inc
<strong>BRDG</strong>-CPLS : Tissue Culture Lab R124, View at Entrance<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 13
<strong>BRDG</strong>-CPLS:Tissue Culture Lab R124, Student Bench View<br />
Plate Washer<br />
Inverted Scope<br />
Water Baths<br />
CO2 Incubators<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 14
<strong>BRDG</strong>-CPLS:Tissue Culture Lab R124, North Counter Bench<br />
CO2 Incubator<br />
Refrigerator<br />
Thermal Cycler<br />
Plate Washer<br />
Water Baths<br />
CO2 /Light<br />
Incubator<br />
(Percival)<br />
Inverted Scope<br />
Large<br />
Incubator<br />
(30cft)<br />
Off frame<br />
Liquid<br />
Handling<br />
(BioMek)<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 15
BioAnalyzers 2x<br />
(Agilent)<br />
<strong>BRDG</strong>-CPLS:Tissue Culture Lab R124, East Counter Bench<br />
Cell Counter<br />
(Bio-Rad)<br />
Smart Cyclers 4x<br />
(Cepheid)<br />
pH Meter<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 16
Room#<br />
<strong>BRDG</strong>-CPLS Lab Systems Corridor, Prep and Equipment Rooms<br />
F<br />
U<br />
M<br />
E<br />
F<br />
U<br />
M<br />
E<br />
Nano<br />
Spect<br />
HAZARD<br />
Storage<br />
126F<br />
ACID<br />
Storage<br />
F<br />
U<br />
M<br />
E<br />
Glass<br />
Wash<br />
AutoClave<br />
Sterilizer<br />
Water<br />
Millipore<br />
Bench<br />
Incubator<br />
ICE<br />
-20<br />
Freezer<br />
-80<br />
Freezer<br />
127 124<br />
Eye Wash<br />
Shower<br />
126B<br />
E 126A Cold<br />
Y<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 126<br />
Room<br />
17<br />
126E<br />
126D<br />
Incubator<br />
E<br />
LN2<br />
tank<br />
Cryo<br />
Dew<br />
ar<br />
BASE<br />
Storage<br />
Cryo<br />
Storage<br />
126C
<strong>BRDG</strong>-CPLS : Corridor; Glasswasher, Water Purification, Ice Maker<br />
GlassWasher<br />
Millipore<br />
Water<br />
Purification<br />
Flake Ice<br />
Maker<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 18
<strong>BRDG</strong>-CPLS: Lab Systems Corridor ; Emergency Wash, Cold Room<br />
Emergency<br />
Eye and<br />
Shower<br />
Station<br />
Eye Wash<br />
Shower<br />
Cold Room<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 19
<strong>BRDG</strong>-CPLS: Lab Systems R126D, Autoclave<br />
Autoclave<br />
(Primus)<br />
w RO System<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 20
<strong>BRDG</strong>-CPLS: Systems Room 126D, -20 Freezer, Incubator, -80 Freezer<br />
-20 Freezer<br />
(VWR)<br />
Large<br />
Incubator<br />
(Sheldon)<br />
-80 Freezer<br />
(So-low)<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 21
<strong>BRDG</strong>-CPLS: Tissue Prep , R126C, Cryostorage<br />
Liquid<br />
Nitrogen<br />
Supply<br />
(Airgas)<br />
Cryostorage<br />
System (VWR)<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 22
I-PC<br />
MACSQuant<br />
Fluorescent<br />
Flow<br />
Cytometer<br />
Floor<br />
Centrifuge<br />
Avanti<br />
J-20XP<br />
(Floor)<br />
<strong>STLCC</strong>-CPLS:Morrison<br />
8/20/2012<br />
Computer Systems: Instrumentation Room Layout<br />
and Network Connections<br />
Auto<br />
MAC<br />
Separator<br />
Incubator<br />
Shaker<br />
(Floor)<br />
1-36<br />
Guest<br />
1-37<br />
Guest<br />
BioPlex<br />
Flow<br />
Cytometer<br />
Cold<br />
Incubator<br />
(Floor)<br />
1-42<br />
Guest<br />
1-43<br />
Unused<br />
P<br />
C<br />
Refrig<br />
Centri<br />
fuge<br />
Strata<br />
gene<br />
Cycler<br />
Open<br />
Guest<br />
L<br />
P<br />
C<br />
126B<br />
Instrumentation<br />
Room<br />
Cen<br />
tri<br />
Vap<br />
Guest<br />
1-39<br />
Guest<br />
ABI<br />
Genetic<br />
Analyzer<br />
Mole-<br />
Cular<br />
Imager<br />
1-39<br />
Guest<br />
1-38<br />
Academic<br />
P<br />
C<br />
P<br />
C<br />
3-34<br />
Guest<br />
Enspire<br />
Reader<br />
Densitometer<br />
(stored below)<br />
Na<br />
no<br />
Dr<br />
op<br />
I-PC<br />
S<br />
C<br />
A<br />
N<br />
1-41<br />
Unused<br />
BP Port # Used<br />
Network<br />
P<br />
C<br />
Link to PC<br />
Beckman<br />
PA-800<br />
Protean<br />
Analyer<br />
1-40<br />
Academic<br />
IPv4: 10.152.30.2<br />
-20<br />
Freezer<br />
(Floor)<br />
Eye Wash<br />
Shower<br />
In Hall<br />
Phone<br />
Page 23
Protein Analyzer<br />
(Beckman)<br />
Nanodrop<br />
Spectroscopy<br />
<strong>BRDG</strong>-CPLS: Instrumentation Room 126B, View Looking NE<br />
Concentrator<br />
Centri-Vap<br />
(Labconco)<br />
Cold Incubator<br />
Molecular<br />
Imager (BioRad)<br />
Centrifuge<br />
(refrigerated, Hermle)<br />
Incubator (Warm)<br />
Shaker/Incubator<br />
Centrifuge<br />
(Avanti)<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 24
<strong>BRDG</strong>-CPLS: Instrumentation Room 126B, View Looking SE<br />
Protein Analyzer<br />
(PA800-Beckman)<br />
Scanner<br />
(Umax)<br />
Molecular<br />
Imager (BioRad)<br />
Incubator<br />
(warm)<br />
Nanodrop<br />
Spectrophotometer<br />
Concentrator<br />
Centri-Vap<br />
(Labconco)<br />
Centrifuge<br />
(refrigerated)<br />
Shaker/Incubator<br />
Centrifuge<br />
(Avanti)<br />
Cold Incubator<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 25
<strong>BRDG</strong>-CPLS: Instrumentation Room 126B, View Looking NW<br />
Array Reader<br />
Flow Cytometer<br />
(BioPlex)<br />
autoMACS<br />
Separator<br />
(Miltenyi)<br />
MACSQuant<br />
Analyzer<br />
(Miltenyi)<br />
Thermal Cycler<br />
(Stratagene)<br />
Genetic Analyzer<br />
(ABI)<br />
Laser Printer<br />
-20 Freezer<br />
Enspire Reader<br />
(Perkin-Elmer)<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 26
MACSQuant<br />
Analyzer<br />
(Miltenyi)<br />
autoMACS<br />
Separator<br />
(Miltenyi)<br />
<strong>BRDG</strong>-CPLS: Instrumentation Room 126B, View Looking SW<br />
Array Reader<br />
Flow Cytometer<br />
(BioPlex)<br />
Thermal Cycler<br />
(Stratagene)<br />
Genetic Analyzer<br />
(ABI)<br />
Enspire Reader<br />
(Perkin-Elmer)<br />
Laser Printer<br />
-20 Freezer<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 27
S<br />
C<br />
O<br />
P<br />
E<br />
Spectro<br />
Spectro<br />
Micro<br />
Wave<br />
Micro<br />
Wave<br />
Shaker<br />
Water<br />
Bath<br />
Water<br />
Bath<br />
-20C<br />
(2cuft)<br />
BRGD-CPLS: Molecular Biology Lab , R127 Layout<br />
UV<br />
Linker<br />
Block<br />
Heater<br />
Block<br />
Heater<br />
Instr PC<br />
Doc Camera<br />
8/20/2012 Morrison <strong>STLCC</strong>-CPLS Morrison Slide 28<br />
127<br />
-20 sml<br />
Freezer<br />
-20 sml<br />
Freezer<br />
F<br />
U<br />
M<br />
E<br />
F<br />
U<br />
M<br />
E<br />
pH<br />
Meter<br />
Nano<br />
Spect<br />
Refrige<br />
rator<br />
-20<br />
Freezer
<strong>BRDG</strong>-CPLS : Molecular Lab, R127, at Entrance<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 29
<strong>BRDG</strong>-CPLS : Molecular Lab, R127, Student Bench View<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 30
<strong>BRDG</strong>-CPLS: Molecular Lab, R127, SW Counter Instrumentation<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 31
<strong>BRDG</strong>-CPLS: Molecular Lab, R127, NW Counter, Biosafety , Hoods<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 32
<strong>BRDG</strong>-CPLS: BioProcessing, with Workbench Islands, Oct 2011<br />
Eye Wash<br />
Shower<br />
30”<br />
HPLC<br />
Spec<br />
tro<br />
Bala<br />
nce<br />
C<br />
H<br />
I<br />
L<br />
119 120 122B<br />
Workbench<br />
Workbench<br />
Bio<br />
Flo<br />
115<br />
Workbench<br />
Workbench<br />
Incuba<br />
tor<br />
Table<br />
119<br />
GTable<br />
60” x 30”<br />
Instructor<br />
Desk<br />
26’<br />
Post center<br />
Page<br />
<strong>STLCC</strong>_CPLS;Morrison 8/20/2012 8/20/2012<br />
<strong>STLCC</strong>-CPLS Morrison 33<br />
27.5’’ post center<br />
Workbench<br />
Workbench<br />
C<br />
Workbench<br />
Workbench<br />
GC<br />
(var)<br />
Bio<br />
Flo<br />
110<br />
Bio<br />
Flo<br />
115<br />
Bio<br />
Flo<br />
110<br />
Bio<br />
Flo<br />
110
<strong>BRDG</strong>-CPLS: Bioprocessing Lab R119 at Entrance<br />
Eye Wash<br />
Shower<br />
At<br />
Entrance<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 34
Room#<br />
36’<br />
8/20/2012<br />
27’<br />
<strong>BRDG</strong>-CPLS: Classroom R118,<br />
& Main Conference Room Layouts<br />
115<br />
Laptop<br />
Cart<br />
120A<br />
<strong>STLCC</strong>-CPLS Morrison<br />
116<br />
118<br />
117<br />
Instr PC<br />
Doc<br />
Camera<br />
E<br />
Y<br />
E<br />
119<br />
Eye Wash<br />
Shower<br />
Scope<br />
119<br />
35
<strong>BRDG</strong>-CPLS : Classroom R118 View at Entrance<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 36
<strong>BRDG</strong>-CPLS : Classroom R118, Student View<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 37
<strong>BRDG</strong>-CPLS: Main Conference Room 115<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 38
<strong>BRDG</strong> CPLS: Space in Danforth Greenhouses<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 39
<strong>BRDG</strong> CPLS: Mobil Tech Center<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 40
<strong>STLCC</strong> Biotechnology: Protocols, SOPs, and Education Website<br />
http://users.stlcc.<strong>edu</strong>/departments/fvbio
<strong>STLCC</strong> Biotechnology Website Instruments: SOPs, Protocols<br />
http://users.stlcc.<strong>edu</strong>/departments/fvbio
<strong>STLCC</strong> Biotechnology Website: SOPs, Protocols (continued)<br />
http://users.stlcc.<strong>edu</strong>/departments/fvbio
<strong>STLCC</strong> <strong>BRDG</strong> <strong>Park</strong> Facility<br />
at the Danforth Plant Science Center<br />
Contact: Bob Morrison, <strong>STLCC</strong>-FV, Instrumentation Specialist, Bmorrison@stlcc.<strong>edu</strong><br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison<br />
44
Center for Plant and Life Sciences Organization Chart<br />
Rafael Hernandez<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 45
• Fans<br />
<strong>BRDG</strong> <strong>Park</strong>: Operations and Maintenance Manual<br />
Key Contacts<br />
– Manf: Greenheck<br />
– Rep: H.C. Sharp, 8301 Crest Industrial, STL 63123, 314-351-6900<br />
• Controls<br />
– Manf: JCI<br />
– Rep: Johnson Controls Inc, 2280 Ball Drive, STL 63146, 314-812-4502<br />
• Air Compressor/Vacuum Pump<br />
– Manf: Kobelco<br />
– Rep: HTE Technologies, 2021 Congressional Dr, STL, 314-731-4444<br />
• Phoenix Valves<br />
– Manf: Phoenix<br />
– Rep: Friemel Love, 7704 Big Bend, STL, 314-647-6363<br />
8/20/2012 <strong>STLCC</strong>-CPLS Morrison 46