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<strong>STLCC</strong> Center for Plant and Life Sciences; <strong>BRDG</strong> <strong>Park</strong><br />

Molecular Biology Lab<br />

Instrumentation Room<br />

Faculty Office Classroom and Conf Rm<br />

<strong>STLCC</strong>: Morrison 8/20/2012<br />

Detect Pictures to see Room Layouts<br />

Major Equipment, Instrumentation<br />

and other Photos<br />

Prep and Equipment Rooms<br />

Prepared by : Bob Morrison, Instrumentation Specialist, Bmorrison@stlcc.<strong>edu</strong><br />

Tissue Culture Lab<br />

Bioprocessing Lab<br />

Greenhouse<br />

Space at Danforth<br />

1


<strong>BRDG</strong> <strong>Park</strong> : CPLS Entrance From North <strong>Park</strong>ing Area<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 2


<strong>BRDG</strong> <strong>Park</strong>: CPLS General Layout, Approx 10,500 sqft<br />

Molecular<br />

Lab<br />

Instrumentation<br />

Room<br />

Conference<br />

Prep Rooms<br />

Autoclave<br />

Freezers, Ice<br />

Eye Wash<br />

Shower<br />

Classroom<br />

Tissue<br />

Culture Lab<br />

Faculty<br />

Break Room<br />

Mechanical Entrance<br />

Office/Staff<br />

Eye Wash<br />

Shower<br />

Bioprocessing<br />

Lab<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 3


<strong>BRDG</strong> <strong>Park</strong> : CPLS Main Entrance<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 4


<strong>BRDG</strong> <strong>Park</strong> : CPLS Entrance Hall<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 5


<strong>STLCC</strong> Center for Plant and Life Sciences (CPLS) Office<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 6


BRD <strong>Park</strong>: CPLS Office Reception Area<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 7


<strong>BRDG</strong>: CPLS Informal Conference Area at Entrance<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 8


<strong>BRDG</strong>: CPLS Break/Refreshment Area<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 9


<strong>BRDG</strong>: CPLS Break Room, Vending<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 10


<strong>BRDG</strong>: CPLS Faculty/Staff Office, R123<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 11


Room#<br />

Eye Wash<br />

Shower<br />

126<br />

126A<br />

E<br />

Y<br />

E<br />

<strong>BRDG</strong>-CPLS: Tissue Culture Lab, R124, Layout<br />

126C<br />

Cold<br />

Room<br />

B<br />

I<br />

O<br />

C<br />

A<br />

B<br />

B<br />

I<br />

O<br />

C<br />

A<br />

B<br />

S<br />

C<br />

O<br />

P<br />

E<br />

124A<br />

Electro<br />

Pulsers<br />

125<br />

CRO<br />

Office<br />

Instr<br />

Scope, PC<br />

Doc<br />

Camera<br />

Smart<br />

Cyclers x 4<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 12<br />

124<br />

Spectro<br />

Room #123<br />

Faculty Office<br />

PCR<br />

Dual<br />

Plate<br />

Washer<br />

Water<br />

Bath x 2<br />

Liquid<br />

Handling<br />

BioMek<br />

Robotic<br />

Plant<br />

Inc<br />

CO2<br />

Inc


<strong>BRDG</strong>-CPLS : Tissue Culture Lab R124, View at Entrance<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 13


<strong>BRDG</strong>-CPLS:Tissue Culture Lab R124, Student Bench View<br />

Plate Washer<br />

Inverted Scope<br />

Water Baths<br />

CO2 Incubators<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 14


<strong>BRDG</strong>-CPLS:Tissue Culture Lab R124, North Counter Bench<br />

CO2 Incubator<br />

Refrigerator<br />

Thermal Cycler<br />

Plate Washer<br />

Water Baths<br />

CO2 /Light<br />

Incubator<br />

(Percival)<br />

Inverted Scope<br />

Large<br />

Incubator<br />

(30cft)<br />

Off frame<br />

Liquid<br />

Handling<br />

(BioMek)<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 15


BioAnalyzers 2x<br />

(Agilent)<br />

<strong>BRDG</strong>-CPLS:Tissue Culture Lab R124, East Counter Bench<br />

Cell Counter<br />

(Bio-Rad)<br />

Smart Cyclers 4x<br />

(Cepheid)<br />

pH Meter<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 16


Room#<br />

<strong>BRDG</strong>-CPLS Lab Systems Corridor, Prep and Equipment Rooms<br />

F<br />

U<br />

M<br />

E<br />

F<br />

U<br />

M<br />

E<br />

Nano<br />

Spect<br />

HAZARD<br />

Storage<br />

126F<br />

ACID<br />

Storage<br />

F<br />

U<br />

M<br />

E<br />

Glass<br />

Wash<br />

AutoClave<br />

Sterilizer<br />

Water<br />

Millipore<br />

Bench<br />

Incubator<br />

ICE<br />

-20<br />

Freezer<br />

-80<br />

Freezer<br />

127 124<br />

Eye Wash<br />

Shower<br />

126B<br />

E 126A Cold<br />

Y<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 126<br />

Room<br />

17<br />

126E<br />

126D<br />

Incubator<br />

E<br />

LN2<br />

tank<br />

Cryo<br />

Dew<br />

ar<br />

BASE<br />

Storage<br />

Cryo<br />

Storage<br />

126C


<strong>BRDG</strong>-CPLS : Corridor; Glasswasher, Water Purification, Ice Maker<br />

GlassWasher<br />

Millipore<br />

Water<br />

Purification<br />

Flake Ice<br />

Maker<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 18


<strong>BRDG</strong>-CPLS: Lab Systems Corridor ; Emergency Wash, Cold Room<br />

Emergency<br />

Eye and<br />

Shower<br />

Station<br />

Eye Wash<br />

Shower<br />

Cold Room<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 19


<strong>BRDG</strong>-CPLS: Lab Systems R126D, Autoclave<br />

Autoclave<br />

(Primus)<br />

w RO System<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 20


<strong>BRDG</strong>-CPLS: Systems Room 126D, -20 Freezer, Incubator, -80 Freezer<br />

-20 Freezer<br />

(VWR)<br />

Large<br />

Incubator<br />

(Sheldon)<br />

-80 Freezer<br />

(So-low)<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 21


<strong>BRDG</strong>-CPLS: Tissue Prep , R126C, Cryostorage<br />

Liquid<br />

Nitrogen<br />

Supply<br />

(Airgas)<br />

Cryostorage<br />

System (VWR)<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 22


I-PC<br />

MACSQuant<br />

Fluorescent<br />

Flow<br />

Cytometer<br />

Floor<br />

Centrifuge<br />

Avanti<br />

J-20XP<br />

(Floor)<br />

<strong>STLCC</strong>-CPLS:Morrison<br />

8/20/2012<br />

Computer Systems: Instrumentation Room Layout<br />

and Network Connections<br />

Auto<br />

MAC<br />

Separator<br />

Incubator<br />

Shaker<br />

(Floor)<br />

1-36<br />

Guest<br />

1-37<br />

Guest<br />

BioPlex<br />

Flow<br />

Cytometer<br />

Cold<br />

Incubator<br />

(Floor)<br />

1-42<br />

Guest<br />

1-43<br />

Unused<br />

P<br />

C<br />

Refrig<br />

Centri<br />

fuge<br />

Strata<br />

gene<br />

Cycler<br />

Open<br />

Guest<br />

L<br />

P<br />

C<br />

126B<br />

Instrumentation<br />

Room<br />

Cen<br />

tri<br />

Vap<br />

Guest<br />

1-39<br />

Guest<br />

ABI<br />

Genetic<br />

Analyzer<br />

Mole-<br />

Cular<br />

Imager<br />

1-39<br />

Guest<br />

1-38<br />

Academic<br />

P<br />

C<br />

P<br />

C<br />

3-34<br />

Guest<br />

Enspire<br />

Reader<br />

Densitometer<br />

(stored below)<br />

Na<br />

no<br />

Dr<br />

op<br />

I-PC<br />

S<br />

C<br />

A<br />

N<br />

1-41<br />

Unused<br />

BP Port # Used<br />

Network<br />

P<br />

C<br />

Link to PC<br />

Beckman<br />

PA-800<br />

Protean<br />

Analyer<br />

1-40<br />

Academic<br />

IPv4: 10.152.30.2<br />

-20<br />

Freezer<br />

(Floor)<br />

Eye Wash<br />

Shower<br />

In Hall<br />

Phone<br />

Page 23


Protein Analyzer<br />

(Beckman)<br />

Nanodrop<br />

Spectroscopy<br />

<strong>BRDG</strong>-CPLS: Instrumentation Room 126B, View Looking NE<br />

Concentrator<br />

Centri-Vap<br />

(Labconco)<br />

Cold Incubator<br />

Molecular<br />

Imager (BioRad)<br />

Centrifuge<br />

(refrigerated, Hermle)<br />

Incubator (Warm)<br />

Shaker/Incubator<br />

Centrifuge<br />

(Avanti)<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 24


<strong>BRDG</strong>-CPLS: Instrumentation Room 126B, View Looking SE<br />

Protein Analyzer<br />

(PA800-Beckman)<br />

Scanner<br />

(Umax)<br />

Molecular<br />

Imager (BioRad)<br />

Incubator<br />

(warm)<br />

Nanodrop<br />

Spectrophotometer<br />

Concentrator<br />

Centri-Vap<br />

(Labconco)<br />

Centrifuge<br />

(refrigerated)<br />

Shaker/Incubator<br />

Centrifuge<br />

(Avanti)<br />

Cold Incubator<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 25


<strong>BRDG</strong>-CPLS: Instrumentation Room 126B, View Looking NW<br />

Array Reader<br />

Flow Cytometer<br />

(BioPlex)<br />

autoMACS<br />

Separator<br />

(Miltenyi)<br />

MACSQuant<br />

Analyzer<br />

(Miltenyi)<br />

Thermal Cycler<br />

(Stratagene)<br />

Genetic Analyzer<br />

(ABI)<br />

Laser Printer<br />

-20 Freezer<br />

Enspire Reader<br />

(Perkin-Elmer)<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 26


MACSQuant<br />

Analyzer<br />

(Miltenyi)<br />

autoMACS<br />

Separator<br />

(Miltenyi)<br />

<strong>BRDG</strong>-CPLS: Instrumentation Room 126B, View Looking SW<br />

Array Reader<br />

Flow Cytometer<br />

(BioPlex)<br />

Thermal Cycler<br />

(Stratagene)<br />

Genetic Analyzer<br />

(ABI)<br />

Enspire Reader<br />

(Perkin-Elmer)<br />

Laser Printer<br />

-20 Freezer<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 27


S<br />

C<br />

O<br />

P<br />

E<br />

Spectro<br />

Spectro<br />

Micro<br />

Wave<br />

Micro<br />

Wave<br />

Shaker<br />

Water<br />

Bath<br />

Water<br />

Bath<br />

-20C<br />

(2cuft)<br />

BRGD-CPLS: Molecular Biology Lab , R127 Layout<br />

UV<br />

Linker<br />

Block<br />

Heater<br />

Block<br />

Heater<br />

Instr PC<br />

Doc Camera<br />

8/20/2012 Morrison <strong>STLCC</strong>-CPLS Morrison Slide 28<br />

127<br />

-20 sml<br />

Freezer<br />

-20 sml<br />

Freezer<br />

F<br />

U<br />

M<br />

E<br />

F<br />

U<br />

M<br />

E<br />

pH<br />

Meter<br />

Nano<br />

Spect<br />

Refrige<br />

rator<br />

-20<br />

Freezer


<strong>BRDG</strong>-CPLS : Molecular Lab, R127, at Entrance<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 29


<strong>BRDG</strong>-CPLS : Molecular Lab, R127, Student Bench View<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 30


<strong>BRDG</strong>-CPLS: Molecular Lab, R127, SW Counter Instrumentation<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 31


<strong>BRDG</strong>-CPLS: Molecular Lab, R127, NW Counter, Biosafety , Hoods<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 32


<strong>BRDG</strong>-CPLS: BioProcessing, with Workbench Islands, Oct 2011<br />

Eye Wash<br />

Shower<br />

30”<br />

HPLC<br />

Spec<br />

tro<br />

Bala<br />

nce<br />

C<br />

H<br />

I<br />

L<br />

119 120 122B<br />

Workbench<br />

Workbench<br />

Bio<br />

Flo<br />

115<br />

Workbench<br />

Workbench<br />

Incuba<br />

tor<br />

Table<br />

119<br />

GTable<br />

60” x 30”<br />

Instructor<br />

Desk<br />

26’<br />

Post center<br />

Page<br />

<strong>STLCC</strong>_CPLS;Morrison 8/20/2012 8/20/2012<br />

<strong>STLCC</strong>-CPLS Morrison 33<br />

27.5’’ post center<br />

Workbench<br />

Workbench<br />

C<br />

Workbench<br />

Workbench<br />

GC<br />

(var)<br />

Bio<br />

Flo<br />

110<br />

Bio<br />

Flo<br />

115<br />

Bio<br />

Flo<br />

110<br />

Bio<br />

Flo<br />

110


<strong>BRDG</strong>-CPLS: Bioprocessing Lab R119 at Entrance<br />

Eye Wash<br />

Shower<br />

At<br />

Entrance<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 34


Room#<br />

36’<br />

8/20/2012<br />

27’<br />

<strong>BRDG</strong>-CPLS: Classroom R118,<br />

& Main Conference Room Layouts<br />

115<br />

Laptop<br />

Cart<br />

120A<br />

<strong>STLCC</strong>-CPLS Morrison<br />

116<br />

118<br />

117<br />

Instr PC<br />

Doc<br />

Camera<br />

E<br />

Y<br />

E<br />

119<br />

Eye Wash<br />

Shower<br />

Scope<br />

119<br />

35


<strong>BRDG</strong>-CPLS : Classroom R118 View at Entrance<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 36


<strong>BRDG</strong>-CPLS : Classroom R118, Student View<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 37


<strong>BRDG</strong>-CPLS: Main Conference Room 115<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 38


<strong>BRDG</strong> CPLS: Space in Danforth Greenhouses<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 39


<strong>BRDG</strong> CPLS: Mobil Tech Center<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 40


<strong>STLCC</strong> Biotechnology: Protocols, SOPs, and Education Website<br />

http://users.stlcc.<strong>edu</strong>/departments/fvbio


<strong>STLCC</strong> Biotechnology Website Instruments: SOPs, Protocols<br />

http://users.stlcc.<strong>edu</strong>/departments/fvbio


<strong>STLCC</strong> Biotechnology Website: SOPs, Protocols (continued)<br />

http://users.stlcc.<strong>edu</strong>/departments/fvbio


<strong>STLCC</strong> <strong>BRDG</strong> <strong>Park</strong> Facility<br />

at the Danforth Plant Science Center<br />

Contact: Bob Morrison, <strong>STLCC</strong>-FV, Instrumentation Specialist, Bmorrison@stlcc.<strong>edu</strong><br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison<br />

44


Center for Plant and Life Sciences Organization Chart<br />

Rafael Hernandez<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 45


• Fans<br />

<strong>BRDG</strong> <strong>Park</strong>: Operations and Maintenance Manual<br />

Key Contacts<br />

– Manf: Greenheck<br />

– Rep: H.C. Sharp, 8301 Crest Industrial, STL 63123, 314-351-6900<br />

• Controls<br />

– Manf: JCI<br />

– Rep: Johnson Controls Inc, 2280 Ball Drive, STL 63146, 314-812-4502<br />

• Air Compressor/Vacuum Pump<br />

– Manf: Kobelco<br />

– Rep: HTE Technologies, 2021 Congressional Dr, STL, 314-731-4444<br />

• Phoenix Valves<br />

– Manf: Phoenix<br />

– Rep: Friemel Love, 7704 Big Bend, STL, 314-647-6363<br />

8/20/2012 <strong>STLCC</strong>-CPLS Morrison 46

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