Cryopreservation and Freeze-Drying Protocols, Second Edition

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Table 1
Standards That May Apply to Biological Resource Centers
Typical methods for Typical methods for Typical methods for
Characteristic bacterial strains plant germplasm animal cell lines
Purity Broth and agar culture to Broth and agar culture to reveal Broth and agar culture to show
reveal pure growth absence of bacteria and fungi no growth of bacteria and
fungi (12)
Tests for mycoplasma (12) and viral
contamination (e.g., electron
microscopy, molecular detection,
inoculation of animals or test cell
lines) (13)
Authenticity Gram stain reaction, colony Morphology of cells/structures, DNA fingerprint, karyology,
morphology, biochemical genotype, expression profile of isoenzyme analysis, cytochrome c
genotyping, biochemical “secondary products,” DNA oxidase sequence (4)
reactions, ribosomal
gene sequence
genotype (10)
Stability/functional Antibiotic susceptibility, Retain ability to regenerate plants, Antibody secretion by hybridomas,
capacity (cultures absence of mutations by sustained expression of susceptibility to viruses,
recovered from cell gene sequencing “secondary products,” absence karyology
banks reproducibly
show key functions)
of “sports” (10,11)