Cryopreservation and Freeze-Drying Protocols, Second Edition

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M E T H O D S I N M O L E C U L A R B I O L O G Y Cryopreservation and Freeze-Drying Protocols Second Edition Edited by John G. Day Culture Collection of Algae and Protozoa Scottish Association for Marine Science Dunstaffnage Marine Laboratory Dunbeg, Argyll, UK Glyn N. Stacey Division of Cell Biology and Imaging National Institute for Biological Standards and Control Potters Bar, Herts., UK
Page 1: METHODS IN MOLECULAR BIOLOGY 368 C
Page 5: M E T H O D S I N M O L E C U L A R
Page 9: Preface The preservation of biologi
Page 12 and 13: viii Contents 15 Cryopreservation o
Page 14 and 15: x Contributors SHARON J. PAYNTER
Page 16 and 17: 1 Long-Term Ex Situ Conservation of
Page 18 and 19: Biological Resource Centers 3 Fig.
Page 20 and 21: 5 Table 1 Standards That May Apply
Page 22 and 23: Biological Resource Centers 7 stora
Page 24 and 25: Biological Resource Centers 9 multi
Page 26 and 27: Biological Resource Centers 11 For
Page 28 and 29: Biological Resource Centers 13 21.
Page 31 and 32: 2 The Principles of Freeze-Drying G
Page 33 and 34: Principles of Freeze-Drying 17 3. T
Page 35 and 36: Principles of Freeze-Drying 19 Ofte
Page 37 and 38: Principles of Freeze-Drying 21 form
Page 39 and 40: Principles of Freeze-Drying 23 orie
Page 41 and 42: Principles of Freeze-Drying 25 Fig.
Page 43 and 44: Principles of Freeze-Drying 27 3. T
Page 45 and 46: Principles of Freeze-Drying 29 may
Page 47 and 48: Principles of Freeze-Drying 31 be c
Page 49 and 50: Principles of Freeze-Drying 33 5. D
Page 51 and 52: Principles of Freeze-Drying 35 auth
Page 53 and 54: Principles of Freeze-Drying 37 32.
Page 55 and 56: 3 Principles of Cryopreservation Da
Page 57 and 58:
Principles of Cryopreservation 41 f
Page 59 and 60:
Principles of Cryopreservation 43 F
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Principles of Cryopreservation 49 K
Page 67 and 68:
Principles of Cryopreservation 51 v
Page 69 and 70:
Page 71 and 72:
Principles of Cryopreservation 55 a
Page 73:
Principles of Cryopreservation 57 2
Page 76 and 77:
60 Matejtschuk Proteins were some o
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62 Matejtschuk others, such as mann
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64 Matejtschuk maintained typically
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66 Matejtschuk alone, and the probe
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68 Matejtschuk 8. The collapse temp
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70 Matejtschuk protein denaturation
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72 Matejtschuk 11. May, J. C., Whee
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74 Tindall storage in a fashion tha
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76 Tindall Fig. 2. The process of m
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78 Tindall Fig. 3. The process of h
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80 Tindall conditions is far too ex
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82 Tindall 3.2. Cryopreservation-Ba
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84 Tindall heat the culture and kil
Page 102 and 103:
86 Tindall 2. Either wash the cells
Page 104 and 105:
88 Tindall Fig. 7. Two freeze-drier
Page 106 and 107:
90 Tindall stocks with that of the
Page 108 and 109:
92 Tindall ampoule, without which i
Page 110 and 111:
94 Tindall cultures by centrifugati
Page 112 and 113:
96 Tindall three strains per alumin
Page 115 and 116:
6 Freeze-Drying of Yeast Cultures C
Page 117 and 118:
Freeze-Drying of Yeast Cultures 101
Page 119 and 120:
Page 121 and 122:
Page 123:
Page 126 and 127:
110 Bond However, some improvements
Page 128 and 129:
112 Bond Fig. 1. Sealing polypropyl
Page 130 and 131:
114 Bond 7. Transfer the cooled cry
Page 132 and 133:
116 Bond If only a small number of
Page 135 and 136:
8 Freeze-Drying Fungi Using a Shelf
Page 137 and 138:
Freeze-Drying Fungi 121 3. Methods
Page 139 and 140:
123 Table 2 Phase-Transition Data S
Page 141:
Freeze-Drying Fungi 125 8. Back, J.
Page 144 and 145:
128 Ryan and Smith of a preservatio
Page 146 and 147:
130 Ryan and Smith fungi with some
Page 148 and 149:
132 Ryan and Smith 2.3. Shelf Freez
Page 150 and 151:
134 Ryan and Smith 14. Before stora
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136 Ryan and Smith 7. The phosphoru
Page 154 and 155:
138 Ryan and Smith and -20°C (34)
Page 156 and 157:
140 Ryan and Smith 36. Rey, L. R. (
Page 158 and 159:
142 Day large numbers of cultures,
Page 160 and 161:
144 Day Table 1 BG 11 Medium Stock
Page 162 and 163:
146 Day b. Passive freezing system
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148 Day 10. Cyanobacteria and red a
Page 166 and 167:
150 Day 27. For many algae, particu
Page 169 and 170:
11 Cryopreservation of Plant Cell S
Page 171 and 172:
Cryopreservation of Plant Cell Susp
Page 173 and 174:
Page 175 and 176:
Page 177:
Page 180 and 181:
164 Benson, Harding, and Johnston r
Page 182 and 183:
166 Benson, Harding, and Johnston 3
Page 184 and 185:
168 Benson, Harding, and Johnston t
Page 186 and 187:
170 Benson, Harding, and Johnston d
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Page 190 and 191:
174 Benson, Harding, and Johnston F
Page 192 and 193:
Page 194 and 195:
Page 196 and 197:
Page 198 and 199:
Page 201 and 202:
13 Cryopreservation of Desiccation-
Page 203 and 204:
Cryopreservation of Desiccation-Tol
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Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217:
Page 220 and 221:
204 Kopeika, Kopeika, and Zhang wid
Page 222 and 223:
206 Kopeika, Kopeika, and Zhang a.
Page 224 and 225:
208 Kopeika, Kopeika, and Zhang of
Page 226 and 227:
210 Kopeika, Kopeika, and Zhang usu
Page 228 and 229:
212 Kopeika, Kopeika, and Zhang of
Page 230 and 231:
214 Kopeika, Kopeika, and Zhang Fig
Page 232 and 233:
216 Kopeika, Kopeika, and Zhang P.,
Page 235 and 236:
15 Cryopreservation of Avian Sperma
Page 237 and 238:
Cryopreservation of Avian Spermatoz
Page 239 and 240:
Page 241:
Page 244 and 245:
228 Morris Concomitant research int
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230 Morris 5. Count the cells over
Page 248 and 249:
232 Morris shipped to other countri
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234 Morris When it is necessary to
Page 252 and 253:
236 Morris 21. Stacey, G. N., Bolto
Page 254 and 255:
238 Watt, Austin, and Armitage 1. I
Page 256 and 257:
240 Watt, Austin, and Armitage 18.
Page 258 and 259:
242 Watt, Austin, and Armitage usin
Page 260 and 261:
244 Watt, Austin, and Armitage 3.1.
Page 262 and 263:
Page 264 and 265:
248 Watt, Austin, and Armitage Ther
Page 266 and 267:
250 Watt, Austin, and Armitage and
Page 268 and 269:
252 Watt, Austin, and Armitage Fig.
Page 270 and 271:
254 Watt, Austin, and Armitage 7. R
Page 272 and 273:
Page 274 and 275:
Page 277 and 278:
18 Cryopreservation of Human Embryo
Page 279 and 280:
Cryopreservation of hESCs 263 Never
Page 281 and 282:
Cryopreservation of hESCs 265 2.2.
Page 283 and 284:
Cryopreservation of hESCs 267 3.2.
Page 285 and 286:
Cryopreservation of hESCs 269 13. C
Page 287 and 288:
19 Cryopreservation of Primary Anim
Page 289 and 290:
Primary Animal Cell Cultures 273 3.
Page 291 and 292:
Primary Animal Cell Cultures 275 4.
Page 293 and 294:
Primary Animal Cell Cultures 277 Fi
Page 295 and 296:
Primary Animal Cell Cultures 279 me
Page 297:
Primary Animal Cell Cultures 281 14
Page 300 and 301:
284 Sputtek No. Cell Method Author
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286 Sputtek platelets (frozen in th
Page 304 and 305:
288 Sputtek 7. Heat sealer. 8. Pate
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290 Sputtek 12. Storage of the froz
Page 308 and 309:
292 Sputtek 14. Lift the reusable c
Page 310 and 311:
294 Sputtek 5. Transfer the PC to a
Page 312 and 313:
296 Sputtek 52. Place the bag of pl
Page 314 and 315:
298 Sputtek 2. Generally, the cell
Page 316 and 317:
300 Sputtek References 1. Sputtek,
Page 319 and 320:
21 Cryopreservation of Mammalian Se
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Mammalian Semen 305 egg yolk in the
Page 323 and 324:
Mammalian Semen 307 2. Materials 1.
Page 325 and 326:
Mammalian Semen 309 5. Seminal flui
Page 327:
Mammalian Semen 311 11. Wales, R. G
Page 330 and 331:
314 Paynter and Fuller Fig. 1. Sche
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316 Paynter and Fuller ICSI, first
Page 334 and 335:
318 Paynter and Fuller Fig. 2. Sche
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320 Paynter and Fuller 4. Pipet a m
Page 338 and 339:
322 Paynter and Fuller 10. A piece
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324 Paynter and Fuller 14. Porcu, E
Page 342 and 343:
326 Fuller and Paynter Fig. 1. (Con
Page 344 and 345:
328 Fuller and Paynter The earliest
Page 346 and 347:
330 Fuller and Paynter and water sh
Page 348 and 349:
332 Fuller and Paynter 6. The next
Page 350 and 351:
334 Fuller and Paynter temperature)
Page 352 and 353:
336 Fuller and Paynter 5. The straw
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338 Fuller and Paynter bent through
Page 357 and 358:
Index 341 Index A Algae, cryopreser
Page 359 and 360:
Index 343 Dimethylsulfoxide (DMSO),
Page 361 and 362:
Index 345 sample damage, chill dama
Page 363:
Index 347 V Vapor pressure, lyophil
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