Bifeng Pan

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Conclusions � Nanotechnology-based advanced materials are rapidly expanding development of better medicines. � siRNA –conjugated Nanoparticles can enter into tumor cells and inhibit tumor cells growth . � Nanoscale delivery system creates a new generation of ‘targeted’ therapeutics which can offer multiple levels of selectivity.
Department of Bio-Nano Bio Nano Science and Technology, Shanghai Jiao Tong University
Page 1 and 2:
Nanomaterials-Mediated Transfer of
Page 3 and 4:
Introduction Nanotechnology has tre
Page 5 and 6:
Three dimensional structure Molecul
Page 7 and 8: Dendrimer Coated Magnetite Nanopart
Page 9 and 10: TEM photos G3.0 PAMAM dendrimer coa
Page 11 and 12: Size Distribution Diameter distribu
Page 13 and 14: FTIR analysis FT-IR spectra of (a)
Page 15 and 16: Preparation of dendrimer coated car
Page 17 and 18: TEM photos Dendrimer coated CNT
Page 19 and 20: Dendrimer coated CNT TEM photos
Page 21 and 22: Raman spectroscopy Raman spectra (7
Page 23 and 24: TGA analysis TGA thermograms of den
Page 25 and 26: Gene silence by small interference
Page 27 and 28: Survivin gene silence by siRNA 5 st
Page 29 and 30: Survivin gene introduction (gene ba
Page 31 and 32: Survivin-51 Target: GCAUCUCUACAUUCA
Page 33 and 34: Survivin 261 Target: CUGUCAAGAAGCAG
Page 35 and 36: Negative Control Sense 5’CACCGTTC
Page 37 and 38: shRNA plasmid vector PGPU6/GFP/Neo-
Page 39 and 40: Interaction between shRNA, siRNA an
Page 41 and 42: Schematic illustration of interacti
Page 43 and 44: Optical microscopy observation Phot
Page 45: Cell growth inhibition by MTT assay
Page 48 and 49: RT-PCR analysis The survivin cDNA w
Page 50: FAM labelled shRNA With nanomateria
Page 57: Influence of nanoparticles conjugat
Page 61: Thanks For your attention!
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Bifeng Pan

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