Helmholtz Russian-German Workshop on Systems Biology Moscow ...

18 <strong>Helmholtz</strong>‐<strong>Russian</strong>‐<strong>German</strong> <strong>Workshop</strong> on Systems Biology
Dr. Hauke Busch
Institution Theoretical Bioinformatics, <strong>German</strong> Cancer Research
Center (DKFZ)
Contact Address
Street Address Bioquant, Im Neuenheimer Feld 267
Zip /Postal Code 69120
City Heidelberg
Country <strong>German</strong>y
Phone +49‐6221‐54‐51309
Fax +49‐6221‐54‐51488
E‐Mail h.busch@dkfz.de
Website http://www.dkfz.de/ibios/
Short CV
1992‐1998 Study in Physics at the Darmstadt University of Technology
1999‐2004 PhD at the Darmstadt University of Technology
”Influence of Spatiotemporal Noise on Pattern Formation
in Excitable Media“.
1999‐2002 Member of the Graduate College 340
„Comminication in Biological Systems“
2004‐now Postdoc at DKFZ, Group of Prof. R. Eils,
Division of Theoretical Bioinformatics
Head of Applied Systems Biology Modeling Group
2004‐2007 Postdoc Fellow of the Systems Biology BioMS Initiative
Research Interests
Main research interests:
‐ the influence of noise in biological systems
‐ development of methods for the efficient simulation of stochastic chemical systems in space and
time.
‐ reverse engineering of dynamic gene regulatory networks from high throughput data
‐ optimal experiment design strategies for biologcial systems.
Five most important publications
H. Busch, D. Camacho‐Trullio, K. Breuhahn, P. Angel, R.Eils and A. Szabowski, Gene Network Dynamics
controlling Keratinocyte Migration, submitted.
H. Busch, W. Sandmann and V. Wolf, A numerical aggregation algorithm for the enzymecatalyzed
substrate conversion, in Proceedings of the Int. Conference on Computational Methods in Systems
Biology, Trento, 2006.
H. Busch and R. Eils, Systems Biology, in Encyclopedia of Molecular Cell Biology and
Molecular Medicine, 14, 123, (2005).
H. Busch and M.‐Th. Hütt, Scale‐dependence of spatiotemporal filters inspired by cellular
automata, Int. J. Bifurcation Chaos, 14, 1957, (2004).
H. Busch, J. Garcia‐Ojalvo, and F. Kaiser, Influence of spatiotemporal 1/f_‐noise on structure formation
in excitable media, Proc. SPIE, 5114, 468, (2003).
Contribution to <strong>Helmholtz</strong> <strong>Russian</strong>­<strong>German</strong> workshop on systems biology
I will prepare
( x) a poster
( ) a talk
Title of poster / talk
Gene Network Dynamics Controlling Keratinocyte Migration
Abstract
So far it is not known how to translate large‐scale omic data into a coherent model of cellular
regulation allowing to simulate, predict and control cellular behavior. However, assuming that
information on cell wide behavior is reflected in the gene expression kinetics, we infer a dynamic gene
regulatory network from time series measurements of DNA micro‐array data for well studied
Hepatocyte Growth Factor‐induced migration of primary human keratinocytes. Transforming the
obtained interactions to the level of signaling pathways, we predict in silico and verify in vitro the
necessary and sufficient time‐ordered events that initiate, maintain and stop migration. We show that
pulse‐like activation of the protooncogene receptor Met triggers a responsive state, while time
sequential activation of EGF‐R is required to initiate and maintain migration. Context information for
enhancing, delaying or stopping migration is provided via the activity of the PKA‐signaling pathway.
Our results shed a new light on a continued kinetic interplay of multiple signaling pathways mediating
the transition to a migratory system state over a time course of several hours.
Poster number: 5