Safety information - Eurogentec

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mechanisms of regulation or it is the expression of the miRNAs, which is affected, or
therefore the analysis of the expression of the pre-miRNAs become highly relevant (12).
Reference
1. Bartel, DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004; 116: 281-97
2. Lai, EC. microRNAs: runts of the genome assert themselves. Curr Biol 2003; 13: R925-36
3. Hutvagner, G, McLachlan, J, Pasquinelli, AE, Balint, E, Tuschl, T and Zamore, PD. A cellular function for the RNAinterference
enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 2001; 293: 834-8
4. Lee, Y, Ahn, C, Han, J, Choi, H, Kim, J, Yim, J, Lee, J, Provost, P, Radmark, O, Kim, S and Kim, VN. The nuclear RNase
III Drosha initiates microRNA processing. Nature 2003; 425: 415-9
5. Lee, YS, Nakahara, K, Pham, JW, Kim, K, He, Z, Sontheimer, EJ and Carthew, RW. Distinct roles for Drosophila Dicer-
1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell 2004; 117: 69-81
6. Khvorova, A, Reynolds, A and Jayasena, SD. Functional siRNAs and miRNAs exhibit strand bias. Cell 2003; 115: 209-16
7. Schwarz, DS, Hutvagner, G, Du, T, Xu, Z, Aronin, N and Zamore, PD. Asymmetry in the assembly of the RNAi enzyme
complex. Cell 2003; 115: 199-208
8. Liu, J, Valencia-Sanchez, MA, Hannon, GJ and Parker, R. MicroRNA-dependent localization of targeted mRNAs to
mammalian P-bodies. Nat Cell Biol 2005; 7: 719-23
9. Pauley, KM, Eystathioy, T, Jakymiw, A, Hamel, JC, Fritzler, MJ and Chan, EK. Formation of GW bodies is a consequence
of microRNA genesis. EMBO Rep 2006; 7: 904-10
10. Rehwinkel, J, Behm-Ansmant, I, Gatfield, D and Izaurralde, E. A crucial role for GW182 and the DCP1:DCP2
decapping complex in miRNA-mediated gene silencing. Rna 2005; 11: 1640-7
11. Bruno, I and Wilkinson, MF. P-bodies react to stress and nonsense. Cell 2006; 125: 1036-8
12. Lu, J, Getz, G, Miska, EA, Alvarez-Saavedra, E, Lamb, J, Peck, D, Sweet-Cordero, A, Ebert, BL, Mak, RH,
Ferrando, AA, Downing, JR, Jacks, T, Horvitz, HR and Golub, TR. MicroRNA expression profiles classify
human cancers. Nature 2005; 435: 834-8
Kit contents
The Eurogentec MiRmaid miRNA precursors qRT-PCR primer set (reference
RT-SYMI-025) contains sufficient MiRmaid RT primer MasterMIX to perform 50 RT
(Reverse Transcriptase) reactions, which is enough for 25 different samples. The amount
of cDNA produced after 1 RT reaction should allow performing 96 qPCR reactions.
The qPCR MasterMix Plus for SYBR ® Green I is a 2X concentrated, ready-to use reaction
buffer containing dNTP/dUTP mix, HotGoldStar, UNG, MgCl 2, SYBR ® Green I, stabilizers
and ROX passive reference. An extra tube of 50 mM MgCl 2 is provided. The qPCR
MasterMix Plus for SYBR ® Green I contains enough reagents for performing up to 600-
25 μl reactions.
If for the Real-Time thermocycler that is used another qPCR MasterMix is required, please refer to our
Real-Time qPCR MasterMix compatibility table with each Real-Time thermocyclers in appendix 3.
The 2 MiRmaid qPCR primer plates contain 112 μl of the specific miRNA Reverse and
Forward primers in each tube. These 170 pre-miRNA F and R primer mixes and the
4 positive control mixes are sufficient to perform at least 25 Real-Time qPCR reactions.
If the qPCR analysis should be performed in duplicates please order a second qPCR MasterMix
Plus for SYBR ® Green I separately (reference # RT-SN2X-03+).
Manual for MiRmaid miRNA precursor qRT-PCR Primer Set I www.eurogentec.com I info@eurogentec.com