Dr. Ute Kothe - University of Lethbridge

Dr. Ute Kothe
Assistant Professor
University of Lethbridge
Department of Chemistry and Biochemistry
4401 University Drive
Lethbridge, Alberta
T1K 3M4
Canada
(403) 332 5274
ute.kothe@uleth.ca
EDUCATION
Ph.D. University of Witten/Herdecke, Germany; Biochemistry
“Dissecting the molecular mechanism of aminoacyl-tRNA selection by
the ribosome”, August 2006
Mark: ”summa cum laude”
Part of PhD: Akademiediskurs, January 2006
Mark: “passed with great distinction”
Diplom (M.S.) University of Witten/Herdecke, Germany; Biochemistry
“Functional role of helix D of Elongation Factor Tu during
interaction with the Ribosome“, July 2002
Mark: “very good with great distinction“ (1.0 / 1.0)
Vordiplom (B.S.) Ruhr-University Bochum, Germany; Biochemistry
September 1999
Mark: “very good“ (1.0 / 1.0)
Abitur Gymnasium St. Ursula Dorsten, Germany
June 1996
Mark: “very good” (1.0 / 1.0)
POSITIONS
Since Oct 2006 Assistant Professor in Biochemistry, University of Lethbridge
CAREER INTERRUPTIONS
June – Nov 2009 Maternity Leave
TEACHING
University of Lethbridge:
2007 – 2011 Supervision of research-based independent studies, applied studies,
coop terms and honour theses
2010-2011 Biochemistry 4000: Nanomachines in Biochemistry (2 courses)
2008 – 2011 Biochemistry 2000: Introductory Biochemistry (6 courses)

Curriculum vitae Dr. Ute Kothe
2010 - 2011 Biochemistry 4000: Contemporary Methods in Biochemistry (2
courses)
2008 – 2009 Biochemistry 4000: Enzyme Structure and Mechanism (2 courses)
2008 Biochemistry 4850: Advanced Methods in Biochemistry
2007 Chemistry 2200: Elements of Organic Chemistry II
Biochemistry 4200, Proteins and Nucleic Acids, Lab 1
University of Witten/Herdecke:
2005/2006 5 lectures in biochemistry and physical biochemistry on the graduate
level
2 seminars in Biochemical methods and ethics on the graduate level
2003 to 2006 Supervision of independent studies and diploma (master) theses
Supervision of the graduate practical course in Biochemistry II
FELLOWSHIPS and AWARDS
2011 Minerva Mentoring Award of the Alberta Women’s Science Network
2011/12 Teaching Fellow at CAETL (Center of the Advancement of
Excellence in Teaching and Learning) at the UofL
2007 Altana PhD award of the GBM (German Society for Biochemistry
and Molecular Biology), best PhD in this field in Germany in the year
2006
2006 Alberta Ingenuity Postdoc Fellowship, declined
2005 Poster prize, Faculty of Life Sciences,
University of Witten/Herdecke, Germany
(5% of the PhD students of the faculty, once per year)
2005 PhD student scholarship award, University of Witten/Herdecke,
Germany (5% of all PhD students at the University)
2004 FEBS young scientist travel fellowship, 29 th Congress of the
Federation of European Biochemical Societies and FEBS Forum of
young scientists, Warzaw, Poland
(100 young biochemists of all European countries per year)
2002 to 2005 PhD student scholarship award, “Studienstiftung des
deutschen Volkes“ (German National Academic Foundation)
(300 PhD students of all scientific fields per year, selection based on
“challenging project with potential substantial contribution to science,
academic abilities of the young scientist, character and anticipated
extraordinary contribution towards the general public”)
2000 to 2002 Graduate student scholarship award, “Studienstiftung des
deutschen Volkes” (German National Academic Foundation)
(0.3% of the current student population in Germany, selection based
on “academic excellence of the candidate with initiative and a strong
sense of social responsibility”)
PROFESSIONAL DEVELOPMENT
2007 Participation in a teaching mentoring program offered by the
University of Lethbridge
2011 Instructional Skills Workshop, Aug 15 – 18, 2011, Center of the
Advancement of Excellence in Teaching and learning (CAETL),
University of Lethbridge
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Curriculum vitae Dr. Ute Kothe
FUNDING
2011 University of Lethbridge Research Fund Grant, $ 4,500 for one
year
Title: “Fluorescence studies of RNA binding and folding by the E. coli
pseudouridine synthase TruB”
2010 CIHR Meetings, Planning and Dissemination Grant, $ 4,500 for
one year
Title: “RiboWest Conference 2010”
2009 NSERC Discovery Grant, $ 25,000 per year for five years
Title: “Molecular Mechanism of RNA modification”
2009 Canada Foundation for Innovation (CFI), New Initiatives Fund
(NIF), $ 8,108,014, co-applicant
Title: “Southern Alberta Group for Epigenetic Studies (SAGES) –
Epigenetic Regulation of Cell Memory and Stress Responses”
2008 The Banting Research Foundation, New Investigator Fund,
$ 20,000 for 12 month
Title: “Yeast model system for H/ACA small nucleolar
ribonucleoproteins in ribosome biogenesis”
2008 NSERC Research Capacity Development Grant administered by
Office of Research Services, UofL, $ 40,000 – Salary and Equipment
funds
Title: “Development of a highly-purified system to study RNA
modification”
2008 Human Resources and Skills Development Canada, $ 2,352,
Summer Research Assistant in Biochemistry (CSJ program)
2008 NSERC Research Tools and Instruments (RTI), $ 58,361,
co-applicant (10%) on application of Dr. H.-J. Wieden
Title: “High Performance Liquid Chromatography (HPLC)”
2008 Canada Foundation for Innovation (CFI), Infrastructure Operating
Fund (IOF), $ 26,355 for 5 years
2007 Canada Foundation for Innovation (CFI), Leaders Opportunity
Fund (LOF), Alberta Advanced Education and Technology, and
University of Lethbridge, $ 329,440, infrastructure fund
Title: “Molecular Mechanisms of Small Ribonucleoproteins”
2007 Alberta Employment, Immigration and Industry, $ 4,356
Summer Research Assistant in Biochemistry (S.T.E.P. program)
2007 Additional Start-up Fund, University of Lethbridge, Research
Services, $ 10,000
2006 Start-up Fund University of Lethbridge, $ 85,000
Title: “Functional investigations on small ribonucleoproteins”
SERVICE TO THE COMMUNITY
• Main organizer of RiboWest Conference 2008, 2010 & 2012, June 15 th – 18 th
2008, July 11 – 14 th 2010, and June 10 – 13 th 2012, University of Lethbridge, AB
• Session Chair at RiboWest Conference 2007 and 2011, University of Northern
British Columbia, BC, Canada
• Peer Reviewer for NSERC Discovery Grant (3 applications from 2009 - 2011)
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Curriculum vitae Dr. Ute Kothe
• Peer Reviewer for Journal Biochemistry and Cell Biology (2008 & 2011) and
Methods (2010)
• Peer Reviewer for MITACS Elevate program (2 applications in 2011)
• Participant in CAETL video “Philosophy of Education” (2010) together with Drs.
Robin Bright and Hillary Rodrigues (80 min)
• Individual Science Outreach Activities
o Judge at Science Fair, Medicine Hat, March 24 th , 2007
o Chemistry Activity for High-school students visiting from Creston to support the
recruitment of students to the UofL, May 13 th 2008
o Judge at Medicine Hat Regional Science Fair, March 1 st 2008
o Judge at Lethbridge Regional Science Fair, April 4 th 2008
o Instructor for Girl Guides to obtain Chemistry Badge: session at the UofL (March
12 th 2008) and participation in Girl Guide meeting (April 9 th 2008)
o Science experiments for three Grade four classes at Mike Mountain Horse
Elementary School (May 23 rd 2008)
o Instructor at Science Summer Camp, UofL (August 12 th , 2008)
o Supervision and housing of high-school student (grade 12) from Fort St. John,
northern BC, working on a science fair project from Aug 24 th – Sep 1 st 2008
o Presentation at AHFMR Teachers Workshop, Calgary, July 21 st 2009
o Supervisor in “Operation Minerva”, UofL – promotion of science for girls (April 3 rd
2009)
o Supervisor in “Operation Minerva”, UofL – promotion of science for girls (April 15 th
2010)
o Delivery of a hands-on workshop on Green Fluorescent Protein at “Operation
Minerva”, UofL – promotion of science for girls (April 12 th 2011)
o Video together with Alberta Innovates – Health Solutions on “Sixty Second
Science” (http://www.youtube.com/watch?v=ObhfCN2uzkA) and “Why I Chose
Science” (http://www.youtube.com/watch?v=1wNJGejtj5I) (Summer 2011)
o Presenter at Lethbridge Pathfinders on “Women in Science as an untraditional
career for women” (February 14 th 2011)
o Supervisor of HYRS high-school student who conducted biomedical research in
my lab for six weeks in summer 2011
o Hands-on workshop “DNA extraction from banana” at grade 8 middle school class
(November 29 th 2011)
o Member on advisory board for new rural development science outreach program
of Science Alberta Foundation at University of Lethbridge (since 2011)
• Let’s Talk Science / Bridges to Science outreach activities:
In late 2009, I have founded together with a few colleagues a new science outreach
program at the University of Lethbridge called “Bridges to Sciences” targeting local
high schools. To include graduate student volunteers, we partnered in summer 2010
with the national Let’s Talk Science organization. Since then, I am the lead organizer
of this science outreach program and faculty supervisor for the graduate student
coordinator.
Outcomes:
o More than 13 high school visits in first year (2010) reaching > 350 students
o Already 7 high school visits in fall 2011 reaching ~ 200 students
o Participation at Lethbridge Science Fair 2011 reaching ~ 50 children
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Curriculum vitae Dr. Ute Kothe
o Participation in Operation Minerva 2011
o Showcasing of hands-on science at University of Lethbridge Open House 2011
o Recruitment of teachers including session at South Western Alberta Teachers’
Convention and three teachers’ nights
TRAINING OF STUDENTS
Graduate students 3 M.Sc. candidates (2008 – 2010, 2010 – 2012 and 2011 - 2013)
1 Ph.D. candidate (2009 – 2012)
Honour thesis students: 6
Independent studies by graduate students: 4
Independent studies by undergraduate students: 23
Undergraduate summer & coop students: 11
International exchange students: 3
Diploma students: 2 (co-supervised at University of Witten/Herdecke, Germany)
MEMBERSHIPS
Biophysical Society
RNA The RNA society
CSBMCB Canadian Society of Biochemistry, Molecular & Cellular Biology
CSC Canadian Society for Chemistry
AAAS American Association for the Advancement of Science
IUBMB International Union of Biochemistry and Molecular Biology
FEBS Federation of European Biochemical Societies
GBM Gesellschaft für Biochemie und Molekularbiologie e.V. (Society for
Biochemistry and Molecular Biology), Germany
PUBLICATIONS
(Supervised students are underlined.)
7. Wright, J.R., Keffer-Wilkes, L.C., Dobing, S.R., and Kothe, U. (2011) Pre-steadystate
kinetic analysis of the three Escherichia coli pseudouridine synthases TruB,
TruA and RluA reveals uniformly slow catalysis. RNA, 17, 2074-2084
6. Kothe, U., and Rodnina, M.V. (2007) Reading of cognate and almost-cognate
codons by tRNA Ala with modified uridine in the wobble position. Mol. Cell 25(1),
167-174
5. Kothe, U., and Rodnina, M.V. (2006) Delayed release of inorganic phosphate from
elongation factor Tu following GTP hydrolysis on the ribosome. Biochemistry, 45,
12767-12774
4. Kothe, U., Paleskava, L., Konevega, A.L., and Rodnina, M.V. (2006) Single-step
purification of specific tRNAs by hydrophobic tagging. Analyt. Biochem. 356, 148-
150
3. Savelsbergh, A., Mohr, D., Kothe, U., Wintermeyer, W., and Rodnina, M.V. (2005)
Control of Phosphate Release from Elongation Factor G by Ribosomal Protein
L7/12. EMBO J. 24(24), 4316-4323
2. Diaconu, M.*, Kothe, U.*, Schluenzen, F., Fischer, N., Harms, J., Tonevitski, A.G.,
Stark, H., Rodnina, M.V., and Wahl, M.C. (2005) Structural basis for the Function of
the Ribosomal L7/12 Stalk in Factor Binding and Activation of GTP Hydrolysis. Cell
121(7), 991-1004
*contributed equally to this work
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Curriculum vitae Dr. Ute Kothe
1. Kothe, U., Wieden, H.-J., Mohr, D., and Rodnina, M. V. (2004) Interaction of Helix
D of Elongation Factor Tu with Helices 4 and 5 of Protein L7/12 on the Ribosome.
J. Mol. Biol. 336, 1011-1021
REVIEWS
2. Kothe, U. (2008) Aminoacyl-tRNA-Selektion durch das Ribosom. BIOspektrum, Vol.
1, 105 (invited review for receiving Altana PhD award)
1. Rodnina, M.V., Gromadski, K.B., Kothe, U., and Wieden, H.-J. (2005) Recognition
and selection of tRNA in translation. FEBS Lett. 579, 938-942
BOOK CHAPTER
1. Kothe, U., (2010) Recent Progress on Understanding Ribosomal Protein Synthesis.
In Comprehensive Natural Products II Chemistry and Biology, Section Amino Acids,
Peptides and Proteins, Elsevier: Oxford, UK, 2010, Volume 5, 353-382 (invited)
PRESENTATIONS AT CONFERENCES
(The presenting author is bold, supervised students are underlined.)
63. Keffer-Wilkes, L., Kothe, U. Come together over U – Pseudouridine Synthases
meet RNA. RiboClub Monthly Videoconference, January 16 th , 2012 (oral video
presentation to 20 RNA research labs across Canada)
62. Lee, V., Kamalampeta, R., Kothe, U. Reconstituting a Functinoal Archaeal H/ACA
Heterotrimeric Cbf5-Nop10-Gar1 Ribonucleoprotein Complex in vitro. 5 th Chinook
Symposium for Chemistry and Biochemistry, Lethbridge, AB, September 30 th 2011
61. Kelly, E., Friedt, J., Kothe, U. Fighting Bacteria by Targeting Pseudouridine
Synthases. 5 th Chinook Symposium for Chemistry and Biochemistry, Lethbridge,
AB, September 30 th 2011 (presentation by visiting high-school student)
60. Keffer-Wilkes, L., Wright, J., Kothe, U. Dissecting Pseudouridien Formation
Reveals Uniformly Slow Catalysis. 5 th Chinook Symposium for Chemistry and
Biochemistry, Lethbridge, AB, September 30 th 2011 (1 st Poster Award,
Biochemistry Graduate students)
59. Kamalampeta, R., Kothe, U. Thermodynamic and Kinetic Studies of the Guide
RNA-Independent Function of Cbf5 in Pseudouridien Formation. 5 th Chinook
Symposium for Chemistry and Biochemistry, Lethbridge, AB, September 30 th 2011
58. Friedt, J., Kothe, U. Development of a Fluorescent, Double-labelled tRNA to
Monitor RNA Folding Dynamics. 5 th Chinook Symposium for Chemistry and
Biochemistry, Lethbridge, AB, September 30 th 2011
57. Dawson, N., Keffer-Wilkes, L., Kothe, U. Mutagenesis, Expression, Purification and
Testing of Pseudouridine Synthase Variants. 5 th Chinook Symposium for Chemistry
and Biochemistry, Lethbridge, AB, September 30 th 2011
56. Keffer-Wilkes, L., Wright, J., Kothe, U. Pre-steady-state kinetic analysis of three
pseudouridine synthases. RiboClub Annual Meeting 2011, Sherbrooke, QC,
September 19 th – 21 st , 2011
55. Kothe, U., Kamalampeta, R., Keffer-Wilkes, L., Friedt, J. Critical steps in
pseudouridine formation by both stand-alone and complex pseudouridine
synthases. RiboClub Annual Meeting 2011, Sherbrooke, QC, September 19 th –
21 st , 2011 (oral presentation)
54. Kothe, U. H/ACA small ribonucleoproteins in health and disease. 12 th Young
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Curriculum vitae Dr. Ute Kothe
Scientist Meeting of the German Society for Cell Biology (DGZ) “RNA & Disease”,
Jena, Germany, September 8 th – 10 th , 2011 (invited oral presentation)
53. Kamalampeta, R., Friedt, J., Kothe, U. Kinetic and thermodynamic analysis of Ψ55
formation in tRNA by the archaeal Cbf5-Nop10-Gar1 complex. 7 th Annual RiboWest
Conference, Prince George, BC, July 18 th – 19 th , 2011 (oral presentation)
52. Keffer-Wilkes, L., Wright, J., Kothe, U. Characterization of pseudouridine
formation by Escherichia coli RluA, TruA and TruB using pre-steady-state kinetics.
7 th Annual RiboWest Conference, Prince George, BC, July 18 th – 19 th , 2011 (oral
presentation)
51. Prabhakara, C., Friedt, J., Kamalampeta, R., Kothe, U. Towards the Biochemical
Characterization of Pseudouridine Synthases in Archaea. 7 th Annual RiboWest
Conference, Prince George, BC, July 18 th – 19 th , 2011
50. Friedt, J., Kamalampeta, R., Prabhakara, C., Kothe, U. Effects of protein complex
composition and guide RNA concentration on pseudouridine formation by an
archaeal H/ACA small ribonucleoproteins. 7 th Annual RiboWest Conference, Prince
George, BC, July 18 th – 19 th , 2011 (oral presentation)
49. Kamalampeta, R., Kothe, U. The role of proteins Nop10 and Gar1 for Cbf5catalyzed
pseudouridine formation. RiboClub Monthly Videoconference, June 6 th ,
2011 (oral video presentation to 20 RNA research labs across Canada)
48. Keffer-Wilkes, L., Wright, J.R., Kothe, U. Investigating RluA and TruB
pseudouridylation kinetics. Graduate Students’ Association – 5 th Annual Multi-
Disciplinary Research Conference, Lethbridge, AB, April 16 th , 2011 (oral
presentation, 1. Presentation Award)
47. Kamalampeta, R., Kothe, U. Cbf5-Nop10-Gar1 is an efficient tRNA pseudouridine
synthase in archaea. Graduate Students’ Association – 5 th Annual Multi-Disciplinary
Research Conference, Lethbridge, AB, April 16 th , 2011 (oral presentation)
46. Kamalampeta, R., Kothe, U. Characterization of guide RNA-dependent and
independent activity of archaeal Cbf5. 4 th Chinook Symposium for Chemistry and
Biochemistry, Lethbridge, AB, October 1 st 2010
45. Keffer-Wilkes, L., Kothe, U. Examining RluA catalyzed pseudouridine formation.
4 th Chinook Symposium for Chemistry and Biochemistry, Lethbridge, AB, October
1 st 2010 (2 nd Poster Award, Biochemistry Graduate students)
44. Sieg, C., Kamalampeta, R., Kothe, U. Interaction of archaeal Cbf5-Nop10 complex
with H/ACA guide RNA and tRNA. 4 th Chinook Symposium for Chemistry and
Biochemistry, Lethbridge, AB, October 1 st 2010
43. Comeau, M.-A., Wieden, H.-J., Kothe, U., Bisaillon, M., Abou Elela, S., Lafontaine,
D. Product-determined turnover rate of Rnase III. RiboClub Annual Meeting 2010,
Sherbrooke, QC, September 20 th – 22 nd , 2010
42. Durand, J., Mosimann, S., Kothe, U. How does Nhp2 bind eukaryotic
H/ACAsnoRNAs? 6 th Annual RiboWest Conference, Lethbridge, AB, July 11 th –
14 th , 2010
41. Keffer-Wilkes, L., Kothe U. Investigating RluA’s mechanism of pseudouridine
formation. 6 th Annual RiboWest Conference, Lethbridge, AB, July 11 th – 14 th , 2010
40. Kamalampeta, R., Kothe, U. Towards dissecting the kinetic mechanism of
pseudouridylation by archaeal H/ACA small ribonucleoproteins. 6 th Annual
RiboWest Conference, Lethbridge, AB, July 11 th – 14 th , 2010 (poster award)
39. Wright, J.R., Dobing, S.R., Kothe, U. Kinetic Mechanism of pseudouridine
formation by E. coli TruB. 6 th Annual RiboWest Conference, Lethbridge, AB, July
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Curriculum vitae Dr. Ute Kothe
11 th – 14 th , 2010 (oral presentation)
38. Wright, J.R., Dobing, S.R., Kothe, U. Towards the Kinetic Mechanism of Bacterial
Pseudouridine Synthases. RNA 2010: 15 th Annual Meeting of the RNA Society,
Seattle, USA, June 22 nd – 26 th 2010
37. Kothe, U. Insight into the mechanism of TruB-catalyzed pseudouridine synthesis.
RiboClub Monthly Videoconference, April 12 th 2010
36. Kamalampeta, R., Kothe, U. Towards Dissecting the Mechanism of RNA
Modification by H/ACA small Ribonucleoproteins. Graduate Students’ Association –
4 th Annual Multi-Disciplinary Research Conference, Lethbridge, AB, March 13 th ,
2010
35. Durand, J., Bruder, L., Mosimann, S., Kothe, U. Analysis of the RNA-binding
properties of yeast H/ACA protein Nhp2. 3 rd Chinook Symposium for Chemistry and
Biochemistry, Lethbridge, AB, October 2 nd 2009
34. Shank, R., Kamalampeta, R., Kothe, U. Cbf5, a pseudouridine synthase, acting as
a stand-alone enzyme. 3 rd Chinook Symposium for Chemistry and Biochemistry,
Lethbridge, AB, October 2 nd 2009
33. Kamalampeta, R., Kothe, U. Optimized Purification of archaeal H/ACA proteins
and RNA. 3 rd Chinook Symposium for Chemistry and Biochemistry, Lethbridge, AB,
October 2 nd 2009
32. Bruder, L., Kothe, U. A yeast model system for Dyskeratosis congenita. 3 rd
Chinook Symposium for Chemistry and Biochemistry, Lethbridge, AB, October 2 nd
2009
31. Wright, J.R., Dobing, S.R., Kothe, U. Rapid Kinetic Analysis of Pseudouridine
Formation by TruB. RiboClub Annual Meeting 2009, Sherbrooke, QC, September
21 st – 24 th 2009
30. Durand, J., Bruder, L., Mosimann, S., Kothe, U. Analysis of the RNA-binding
properties of yeast H/ACA protein Nhp2. 5 th Annual RiboWest Conference, Prince
George, BC, July 19 th – 22 nd , 2009
29. Shank, R., Kamalampeta, R., Kothe, U. The pseudouridine synthase Cbf5 acting
as a stand-alone enzyme. 5 th Annual RiboWest Conference, Prince George, BC,
July 19 th – 22 nd , 2009
28. Kamalampeta, R., Kothe, U. Biochemical Studies of Ribonucleoproteins, Graduate
Students’ Association – 3 rd Annual Multi-Disciplinary Research Conference,
Lethbridge, AB, March 14 th , 2009
27. Wright, J., Kothe, U. Studying the kinetic mechanism of pseudouridine formation
by TruB. 43 rd Prairie Universities Biological Symposium (PUBS), Lethbridge, AB,
February 20 th – 21 st 2009 (oral presentation)
26. Durand, J., Kothe, U. Purification of Saccharomyces cerevisiae H/ACA core
proteins and guide RNA for biochemical studies of ribosome biogenesis. 43 rd
Prairie Universities Biological Symposium (PUBS), Lethbridge, AB, February 20 th –
21 st 2009 (oral presentation)
25. Wright, J., Kothe, U. Pseudouridine Formation by TruB. 2 nd Chinook Symposium
for Chemistry and Biochemistry, Lethbridge, AB, October 3 rd 2008
24. Koerselman, C., Kothe, U. Investigating the Protein Core of Archaeal H/ACA small
Ribonucleoproteins. 2 nd Chinook Symposium for Chemistry and Biochemistry,
Lethbridge, AB, October 3 rd 2008
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Curriculum vitae Dr. Ute Kothe
23. Durand, J., Kothe, U. Purification of Saccharomyces cerevisiae H/ACA Proteins.
2 nd Chinook Symposium for Chemistry and Biochemistry, Lethbridge, AB, October
3 rd 2008
22. Kothe, U. Ribosomal discrimination against near-cognate tRNAs. Bielefeld RNA
Structural Dynamics Mini-Symposium, Bielefeld, Germany, August 4 th – 5 th 2008
(invited oral presentation)
21. Kothe, U., Rodnina, M.V. Uniform Accuracy in Ribosomal Aminoacyl-tRNA
Selection. RNA 2008: 13 th Annual Meeting of the RNA Society, Berlin, Germany,
July 28 th – Aug 3 rd 2008
20. Tavernini, L., Koerselman, C., Kothe, U. Archaeal H/ACA small ribonucleoproteins.
4 th Annual RiboWest Conference, Lethbridge, AB, June 15 th – 18 th 2008
19. Durand, J., Dobing, S., Kothe, U. Purification of Saccharomyces cerevisiae H/ACA
core proteins. 4 th Annual RiboWest Conference, Lethbridge, AB, June 15 th – 18 th
2008
18. Wright, J., White, T., Hagstrom, L., Kothe, U. , Pseudouridine Formation by TruB.
4 th Annual RiboWest Conference, Lethbridge, AB, June 15 th – 18 th 2008
17. Kothe, U., Rodnina M. Uniform accuracy in ribosomal protein synthesis. 91 st
Canadian Chemistry Conference, Edmonton, AB, May 24 th – 28 th 2008 (invited
oral presentation)
16. Kothe, U. H/ACA small ribonucleoproteins, dyskeratosis congenita and cancer.
Sixth Annual New Principal Investigators Meeting, Canadian Institutes of Health
Research (CIHR), Jackson’s Point, ON, November 9 th – 11 th 2007
15. Shim, J., Kothe, U. Cloning of four H/ACA proteins for reconstitution of an H/ACA
complex. 1 st Chinook Symposium for Chemistry and Biochemistry, Lethbridge, AB,
October 5 th 2007
14. Tavernini, L., Kothe, U. Cloning and overexpression of proteins involved in the
H/ACA ribonucleoprotein complex. 1 st Chinook Symposium for Chemistry and
Biochemistry, Lethbridge, AB, October 5 th 2007
13. Kothe, U., Rodnina, M.V. Dissecting the molecular mechanism of aminoacyl-tRNA
selection by the ribosome. GBM Fall Meeting – Molecular Life Sciences 2007
(German Society of Biochemistry and Molecular Biology), Hamburg, Germany,
September 16 th – 19 th 2007 (invited oral presentation)
12. Kothe, U. RNA modification as an evolutionary adjustment of the ribosome.
Ribowest Meeting 2007, Prince George, BC, Canada, July 29 th – 31 st , 2007
(oral presentation)
11. Tavernini, L., Shim, J., Kothe, U. Reconstitution of an Archaeal H/ACA
ribonucleoprotein complex for mechanistic studies. Ribowest Meeting 2007, Prince
George, BC, Canada, July 29 th – 31 st , 2007
10. Kothe, U., Rodnina, M.V. Modifying ribosomal decoding by tRNA modifications.
CSBMCB 50 th Annual Meeting, Montreal, QC, Canada, July 5 th – 9 th , 2007
9. Kothe, U., Rodnina, M.V. Extended Codon reading by tRNA with a Uridine 5oxyacetic
acid Modification at Position 34. RNA 2007: 12 th Annual Meeting of the
RNA Society, Madison, WI, U.S.A., May 29 th – June 3 rd , 2007
8. Kothe, U., Gromadski, K.B., Daviter, T., Rodnina, M.V. Uniform Selection of
Aminoacyl-tRNAs by the Ribosome. RNA 2006: 11 th Annual Meeting of the RNA
Society, Seattle, U.S.A., June 20 th – 25 th , 2006
(oral presentation)
7. Rodnina, M.V., Gromadski, K., and Kothe, U. Recognition and Selection of EF-Tu-
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Curriculum vitae Dr. Ute Kothe
GTP-Aminoacyl-tRNA by the Ribosome. 21 st International tRNA Workshop,
Bangalore, India, December 2 nd - 7 th , 2005
6. Savelsbergh, A., Kothe, U., Rodnina, M.V., and Wintermeyer, W. Conformational
Coupling Between EF-G and the Ribosome During Translocation. 21 st International
tRNA Workshop, Bangalore, India, December 2 nd - 7 th , 2005
5. Kothe, U., Diaconu, M., Schluenzen, F., Fischer, N., Harms, J., Tonevitski, A.G.,
Stark, H., Wahl, M.C., and Rodnina, M.V. Structural basis for the Function of the
Ribosomal L7/12 Stalk in Factor Binding and Activation of GTP Hydrolysis. EMBO
Conference on Protein Synthesis and Translational Control, Heidelberg, Germany,
September 14 th – 18 th , 2005
(oral presentation)
4. Rodnina, M.V., Gromadski, K.B., Beringer, M., and Kothe, U. Substrate selection
and catalysis by the ribosome. 15 th IUPAB & 5 th EBSA International Biophysics
Congress, Montpellier, France, August 27 th – September 1 st , 2005
3. Kothe, U., and Rodnina, M.V. Aminoacyl-tRNA Selection at GC-rich codons.
RNA 2005: 10 th Annual Meeting of the RNA Society, Banff, Canada, May 24 th –
29 th , 2005
2. Kothe, U., Wieden, H.-J., Mohr, D., and Rodnina, M. V. Interaction of Helix D of
Elongation Factor Tu with Helices 4 and 5 of Protein L7/12 on the ribosome. 29 th
Congress of the Federation of European Biochemical Societies (FEBS), Warzaw,
Poland, June 26 th – July 1 st , 2004
1. Kothe, U., Wieden, H.-J., Mohr, D., and Rodnina, M. V. Functional
complementarity of Helix D in Elongation Factor Tu and helices 4/5 in the ribosomal
protein L7/12. 20 th International tRNA Workshop, Banz, Germany, October 2 nd - 7 th ,
2003
OTHER ORAL PRESENTATIONS
17. Kothe, U. Pseudouridine formation in tRNA – always the same story? University of
Alberta, Dec 1 st 2011
16. Kothe, U. Challenges and Opportunities of Science Outreach. University of
Lethbridge Women Scholar Speaker Series, Nov 8 th 2011
15. Kothe, U. New insight into RNA modification enzymes. University of Alberta, Oct
18 th 2010
14. Kothe, U. RNA modification: tuning an old molecule for modern pace. University of
Calgary, Feb 13 th 2009
13. Kothe, U. Ribosome and Protein Synthesis. Lethbridge Research Center -
Agriculture and Agri-Food Canada, Feb 27 th 2008
12. Kothe, U. A unifying view at ribosome structure and function. University of
Manitoba, Nov 23 rd 2007
11. Kothe, U. A unifying view at ribosome structure and function. University of
Winnipeg, Nov 22 nd 2007
10. Kothe, U.Ribosome Structure, Function and Biogenesis. University of Alberta, Nov
16 th 2007
9. Kothe, U. Decoding the ribosome by rapid kinetic techniques. University of
Saskatchewan, Oct 19 th 2007
8. Kothe, U. Decoding the ribosome by rapid kinetic techniques. University of Regina,
Oct 18 th 2007
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Curriculum vitae Dr. Ute Kothe
3. Kothe, U. Decoding the ribosome by rapid kinetic techniques. Simon Fraser
University, Aug 9 th 2007
6. Kothe, U. Decoding the ribosome by rapid kinetic techniques. University of British
Columbia, Aug 8 th 2007
5. Kothe, U. How the ribosome deals with the decoding problem. Department of
Biological Sciences, University of Lethbridge, AB, Canada, February 8 th , 2007
4. Kothe, U. Is, Ought, Can: ethics from an evolutionary perspective.
Akademiediskurs (part of the Ph.D. thesis), University of Witten/Herdecke,
Germany, January 28 th , 2006
3. Kothe, U. The naturalistic fallacy in evolutionary ethics. “Ellipse” (Philosophy
association), Essen, Germany, January 10 th , 2006
2. Kothe, U., and Rodnina, M.V. Functional complementarity of helix D in elongation
factor Tu and helices 4/5 in the ribosomal protein L7/12. Biochemistry Ph.D.
students meeting, Pottenstein, Germany, June 19 th - 21 st , 2003
1. Kothe, U., and Rodnina, M.V. Importance of Helix D of Elongation Factor Tu for
Binding to the Ribosome. Biochemistry Ph.D. students meeting, Pottenstein,
Germany, May 30 th – June 1 st , 2002
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