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Membrane Technology for Enzyme Separations - NBV

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<strong>Membrane</strong> <strong>Technology</strong> <strong>for</strong> <strong>Enzyme</strong> <strong>Separations</strong><br />

DSM<br />

First experiments<br />

1<br />

2<br />

3<br />

Feed: Effluent of reactor containing (immobilized) enzyme<br />

Equipment: Tubular membrane, area about 0,01 m 2 , cut-off 9 kD,<br />

PolyEtherSulphon membrane (PCI). Cross flow velocity: 0.25 m/s.<br />

Conditions: 3 bar, 5 °C. Concentration factor about 2.<br />

Repeat with same feed.<br />

Same equipment.<br />

Conditions: 6 bar, 5 °C. Concentration factor about 4.<br />

Repeat with feed containing (much) more proteins (worst case).<br />

Tubular membrane, area about 0,01 m 2 , cut-off 8 kD,<br />

PolySulphone membrane (PCI). Cross flow velocity: 0,25 m/s.<br />

Conditions: 6 bar, 5 °C. Concentration factor between 4 and 5.

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