Membrane Technology for Enzyme Separations - NBV
Membrane Technology for Enzyme Separations - NBV
Membrane Technology for Enzyme Separations - NBV
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<strong>Membrane</strong> <strong>Technology</strong> <strong>for</strong> <strong>Enzyme</strong> <strong>Separations</strong><br />
DSM<br />
First experiments<br />
1<br />
2<br />
3<br />
Feed: Effluent of reactor containing (immobilized) enzyme<br />
Equipment: Tubular membrane, area about 0,01 m 2 , cut-off 9 kD,<br />
PolyEtherSulphon membrane (PCI). Cross flow velocity: 0.25 m/s.<br />
Conditions: 3 bar, 5 °C. Concentration factor about 2.<br />
Repeat with same feed.<br />
Same equipment.<br />
Conditions: 6 bar, 5 °C. Concentration factor about 4.<br />
Repeat with feed containing (much) more proteins (worst case).<br />
Tubular membrane, area about 0,01 m 2 , cut-off 8 kD,<br />
PolySulphone membrane (PCI). Cross flow velocity: 0,25 m/s.<br />
Conditions: 6 bar, 5 °C. Concentration factor between 4 and 5.