Organelle Isolation and Marker Enzyme Assay - Association for ...
Organelle Isolation and Marker Enzyme Assay - Association for ...
Organelle Isolation and Marker Enzyme Assay - Association for ...
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146 <strong>Organelle</strong> <strong>Isolation</strong><br />
Instruments <strong>for</strong> the exercise:<br />
Clinical bench-top centrifuge<br />
Sorvall RC-5B/RC-2B-type or comparable refrigerated centrifuges which can use Sorvall SS34-type<br />
or similar rotors. (A microfuge could be used with appropriate speed [rpm] equivalent to the<br />
desired RCF-min.)<br />
SS34, or similar, rotors<br />
Water baths (37°C)<br />
Refrigerator (1)<br />
Spectronic 20 (or similar) spectrophotometers<br />
Instruments <strong>for</strong> growing cells:<br />
Gyratory shaker<br />
Incubator at 22°C (if room temperature is not controlled)
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