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Thermo Scientific BioLite Cell Culture Products - Fisher Scientific

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Doubling Time (hours)<br />

(mean ± SD)<br />

Doubling Time (hours)<br />

(mean ± SD)<br />

Doubling Time <strong>Cell</strong> of Enumeration<br />

HEK <strong>Cell</strong>s on Surfaces from<br />

Doubling Time of HEK <strong>Cell</strong>s on Surfaces from Different Manufacturers<br />

Various Manufacturers<br />

30 –<br />

25 –<br />

20 –<br />

15 –<br />

10 –<br />

5 –<br />

0 –<br />

<strong>Thermo</strong><br />

<strong>Scientific</strong><br />

<strong>BioLite</strong> <strong>Products</strong><br />

Doubling Time <strong>Cell</strong> of VERO Enumeration <strong>Cell</strong>s on Surfaces from<br />

Doubling Time of VERO Various <strong>Cell</strong>s on Manufacturers<br />

Surfaces from Different Manufacturers<br />

30 –<br />

25 –<br />

20 –<br />

15 –<br />

10 –<br />

5 –<br />

0 –<br />

<strong>Thermo</strong><br />

<strong>Scientific</strong><br />

<strong>BioLite</strong> <strong>Products</strong><br />

<strong>Cell</strong> Enumeration<br />

G T CT<br />

<strong>Cell</strong> Enumeration<br />

G T CT<br />

Conclusions<br />

In the study conducted, the performance of <strong>Thermo</strong><br />

<strong>Scientific</strong> <strong>BioLite</strong> products was compared to those<br />

of other manufacturers for growth of HEK (293)<br />

and VERO cells. Performance of <strong>BioLite</strong> products<br />

was indistinguishable from the other brands both<br />

in terms of doubling time and cell morphology<br />

for both HEK and VERO cells. The results suggest<br />

that comparable results may be obtained when<br />

switching from any of the surfaces compared above<br />

to the <strong>BioLite</strong> products.<br />

<strong>Cell</strong> Yield of VERO <strong>Cell</strong> <strong>Cell</strong>s Yield on Surfaces from<br />

<strong>Cell</strong> Yield of VERO Various <strong>Cell</strong>s on Surfaces Manufacturers from Different Manufacturers<br />

Visit www.thermoscientific.com/biolite for additional information.<br />

© 2010 <strong>Thermo</strong> <strong>Fisher</strong> <strong>Scientific</strong> Inc. All rights reserved. All trademarks are the property of<br />

<strong>Thermo</strong> <strong>Fisher</strong> <strong>Scientific</strong> Inc. and its subsidiaries.<br />

BN0518107/BRLSPCCGTSBIOLITE 0710<br />

<strong>Cell</strong> Yield (cells/cm 2 ) (mean ± SD)<br />

<strong>Cell</strong> Yield (cells/cm 2 ) (mean ± SD)<br />

<strong>Cell</strong> Yield of HEK <strong>Cell</strong> <strong>Cell</strong>s Yield on Surfaces from<br />

<strong>Cell</strong> Yield of HEK <strong>Cell</strong>s on Surfaces from Different Manufacturers<br />

Various Manufacturers<br />

200,000 –<br />

150,000 –<br />

100,000 –<br />

50,000 –<br />

200,000 –<br />

150,000 –<br />

100,000 –<br />

50,000 –<br />

0 –<br />

<strong>Thermo</strong><br />

<strong>Scientific</strong><br />

<strong>BioLite</strong> <strong>Products</strong><br />

0 –<br />

<strong>Thermo</strong><br />

<strong>Scientific</strong><br />

<strong>BioLite</strong> <strong>Products</strong><br />

<strong>Cell</strong> Yield<br />

G T CT<br />

<strong>Cell</strong> Yield<br />

G T CT<br />

Surface modifications meant to improve cell<br />

adhesion produce slightly different surfaces from<br />

one manufacturer to the next. <strong>Cell</strong> adhesion,<br />

morphology, and growth rate may vary, often<br />

significantly, when switching from one surface to<br />

another. Because it isn’t possible to predict the<br />

behavior of any given cell line on a new surface,<br />

this must be validated by experimentation.<br />

www.thermoscientific.com/biolite

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