Reliable separation and analysis of large-size proteins ... - Invitrogen

dependable tools for large
protein analysis
SDS-PAGE is the most commonly used
method to separate and analyze protein
samples. Unfortunately, inconsistent band
resolution, the limited shelf life inherent in
Laemmli gels, and the lack of high molecular
weight protein standards often leads to
variable and inaccurate results when working
with proteins >200 kDa. Now you can
overcome these challenges with the
NuPAGE ® Novex Tris-Acetate Gel/SDS Buffer
System and HiMark Unstained High
Molecular Weight Protein Standard. The
assigned molecular weight values of the
HiMark Standard proteins are precisely
established in the Tris-Acetate denaturing
system. You’ll reliably analyze big proteins
in a pre-cast mini-gel SDS-PAGE format and
improve your analysis accuracy.
pre-cast gel system offers
high-resolution
The NuPAGE ® Novex Tris-Acetate Gel/SDS
Buffer System is a revolutionary polyacrylamide
gel system specifically designed for
clear separation of high molecular weight
proteins. It employs neutral pH gel/buffer
chemistry to increase gel stability and minimize
protein modifications, which can occur
in the Tris-Glycine Gel electrophoresis. With
the Tris-Acetate Gels and pre-optimized
buffers, you’ll get well-resolved, intact bands
and consistent resolution for your sample
and standard proteins every time (figure 1).
HiMark Unstained Protein Standard & NuPAGE ® Tris-Acetate Gel System
Reliable separation and analysis
of large-size proteins in SDS-PAGE
L
arge proteins, such as nuclear proteins in human cells, play important roles in biological
pathways. Using the new HiMark Unstained High Molecular Weight Protein Standard
along with the NuPAGE ® Tris-Acetate Gels provides you with a convenient and trustworthy
method to analyze large proteins.
figure 1 - sharp HiMark Standard bands
on NuPAGE ® Tris-Acetate Gels
kDa
500
290
240
160
116
97
66
55
40
1 2 kDa 1 2
460 kDa- 500
kinase 290
240
160
116
97
NuPAGE ® 3-8% Tris-
Acetate Gel/Tris-
Acetate SDS buffer
the only marker set for
large protein analysis
The HiMark Standard is the only high
molecular weight marker designed for SDS-
PAGE. It consists of nine proteins that
migrate with apparent molecular weights of
40, 55, 66, 97, 116, 160, 240, 290, 500 kDa
in NuPAGE ® Novex Tris-Acetate Gels with
Tris-Acetate SDS buffer (figure 1, lane 1).
The innovative technology ensures sharp,
tight band resolution from run to run.
Visualize standard bands using Coomassie ®,
silver, or other protein stains. You can easily
establish the standard curve and estimate
molecular weights of your large proteins.
There’s no need to extrapolate the standard
curve, resulting in correct size estimations.
66
55
40
NuPAGE ® 7% Tris-
Acetate Gel/Tris-
Acetate SDS buffer
lane 1: 5 µl of HiMark Standard
lane 2: 4 µl of protein sample
Gels stained with Coomassie ® R-250 stain
460 kDakinase
count on these two for your success
Together, the NuPAGE ® Tris-Acetate Gels and
HiMark Standard provide you with the first
and only method for reliably analyzing large
proteins. Succeed in your large protein characterization
and identification studies by
ordering the HiMark Standard and NuPAGE ®
Tris-Acetate Gel/Buffer System today.
Product Quantity Cat. no.
HiMark Unstained High Molecular Weight
Protein Standard 250 µl LC5688
NuPAGE ® Novex 7% Tris-Acetate Gel*
1.0 mm, 10 well 1 box EA0355BOX
NuPAGE ® Novex 3-8% Tris-Acetate Gel*
1.0 mm, 10 well 1 box EA0375BOX
NuPAGE ® Tris-Acetate SDS Buffer Kit
contains 1 ea. NuPAGE ® sample buffer, reducing
agent, antioxidant, and Tris-Acetate SDS running
buffer
XCell SureLock
1 kit LA0050
Mini-Cell †
1 unit EI0001
* Each box contains 10 gels. Other well format and thickness
of Tris-Acetate Gels are also available.
† A convenient mini electrophoresis apparatus specifically
designed for running Novex ® Gels.
These products may be covered by one or more Limited Use Label Licenses
(See the Invitrogen catalog or web site, www.invitrogen.com). By the use of
these products you accept the terms and conditions of all applicable Limited
Use Label Licenses.
1600 Faraday Avenue • Carlsbad CA 92008 USA
Tel: 800 955 6288 • Fax: 760 603 7229
www.invitrogen.com

HiMark Unstained Protein Standard & NuPAGE ® Tris-Acetate Gel System
HiMark Unstained Protein Standard
High Molecular Weight Standard for Large Protein Analysis on NuPAGE ® Tris-Acetate Gels
Description:
The HiMark Unstained High Molecular Weight Protein Standard is specifically designed for large protein analysis on NuPAGE ® Novex
Tris-Acetate Gels under denaturing conditions. It consists of nine proteins, ranging in size from 40-500 kDa in NuPAGE ® Novex 3-8% and
7% Tris-Acetate Gel/SDS buffer systems. Visualize with Coomassie ® stain, silver, and other protein stains. The HiMark Standard offers:
• Nine sharp bands that resolve clearly on NuPAGE ® Novex Tris-Acetate Gels (Figure 1)
• Detection with Coomassie ® or silver staining post-electrophoresis
• Detection with SYPRO ® Ruby Blot stain (Figure 2), Coomassie ®, Ponceau S, or other membrane stain post-transferring
• A time-saving load-and-go format
• A downloadable molecular weight calculator for easy and accurate MW estimation of large proteins*
Figure 1 - HiMark Standard bands on
NuPAGE ® Tris-Acetate Gels
kDa
500
290
240
160
116
97
66
55
40
1 2 kDa 1 2
460 kDa- 500
kinase
290
240
160
116
97
NuPAGE ® 3-8% Tris-
Acetate Gel/Tris-
Acetate SDS buffer
66
55
40
NuPAGE ® 7% Tris-
Acetate Gel/Tris-
Acetate SDS buffer
Lane 1: 5 µl of HiMark Standard
Lane 2: 4 µl of protein sample
Gels stained with Coomassie ® R-250 stain.
460 kDakinase
Contents and Storage:
250 µl (50 applications of 5 µl each) is provided in loading buffer consisting of Tris-HCl, Tris-base, LDS, EDTA, Glycerol, DTT, SERVA ® Blue
G-250, and Phenol Red. Store at -20˚C. Avoid repeated freezing and thawing. Stable for 6 months when properly stored.
Product Quantity Cat. no.
HiMark Unstained Protein Standard 250 µl LC5688
*For more information on the HiMark MW Calculator, visit Invitrogen web site at www.invitrogen.com.
Figure 2 - Blot of HiMark Standard and protein sample
stained with SYPRO ® Ruby Blot Stain
Standard and protein sample were separated on a NuPAGE ® 3-8%
Tris-Acetate Gel/SDS buffer and transferred to an Invitrolon PVDF
membrane, then stained with SYPRO ® Ruby Blot Stain according to
the protocol (Molecular Probes). AlphaImager 1/8 –sec. exposure.
Lane 1: 5 µl of HiMark Standard
Lane 2: 4 µl of protein sample
460 kDakinase
1600 Faraday Avenue, Carlsbad CA 92008 USA Tel: 800 955 6288 Fax: 760 603 7229 E-mail: tech_service@invitrogen.com www.invitrogen.com
For research use only. Not intended for any animal or human therapeutic or diagnostic use. Printed in the U.S.A. ©2003 Invitrogen Corporation. All rights reserved. Reproduction forbidden without permission. These products may be covered by
one or more Limited Use Label Licenses (See the Invitrogen catalog or our web site, www.invitrogen.com). By the use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses.
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713-038155 092503 3.5M