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Fluorescent and colorimetric labeling and detection - Invitrogen

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<strong>Fluorescent</strong> <strong>and</strong> <strong>colorimetric</strong> <strong>labeling</strong> <strong>and</strong> <strong>detection</strong><br />

Zenon® Technology<br />

Zenon® Technology eliminates the need for secondary <strong>detection</strong> reagents in many applications, <strong>and</strong><br />

simplifies immuno<strong>labeling</strong> applications that were previously time consuming or impractical. The Zenon®<br />

<strong>labeling</strong> method takes advantage of the immunoselectivity of the antibody binding reaction by forming a<br />

complex between an intact primary IgG antibody <strong>and</strong> a fluorophore-, biotin- or enzyme-labeled Fab fragment<br />

directed against the Fc portion of that IgG. Simple mixing of the labeled Fab fragment, supplied in the<br />

Zenon® Antibody Labeling Kits, with the corresponding primary antibody quantitatively produces the Fabantibody<br />

complex in under 10 minutes, with no pre- or post-<strong>labeling</strong> purification required. Labeled Fabantibody<br />

complexes can be used immediately to stain cells, tissues, <strong>and</strong> other targets.<br />

Product Species Quantity Cat.<br />

no.<br />

Zenon® Alexa Fluor® 488 Goat IgG Labeling Kit , 50 <strong>labeling</strong>s Goat 1 kit Z-25602<br />

Zenon® Alexa Fluor® 555 Goat IgG Labeling Kit, 50 <strong>labeling</strong>s Goat 1 kit Z-25605<br />

Zenon® Alexa Fluor® 594 Goat IgG Labeling Kit, 50 <strong>labeling</strong>s Goat 1 kit Z-25607<br />

Zenon® Alexa Fluor® 647 Goat IgG Labeling Kit , 50 <strong>labeling</strong>s Goat 1 kit Z-25608<br />

Zenon® Alexa Fluor® 568 Goat IgG Labeling Kit , 50 <strong>labeling</strong>s Goat 1 kit Z-25606<br />

Zenon® Alexa Fluor® 350 Human IgG Labeling Kit, 50 <strong>labeling</strong>s Human 1 kit Z-25400<br />

Zenon® Alexa Fluor® 488 Human IgG Labeling Kit , 50 <strong>labeling</strong>s Human 1 kit Z-25402<br />

Zenon® Alexa Fluor® 555 Human IgG Labeling Kit , 50 <strong>labeling</strong>s Human 1 kit Z-25405<br />

Zenon® Alexa Fluor® 594 Human IgG Labeling Kit , 50 <strong>labeling</strong>s Human 1 kit Z-25407<br />

Zenon® Alexa Fluor® 647 Human IgG Labeling Kit , 50 <strong>labeling</strong>s Human 1 kit Z-25408<br />

Zenon® Allophycocyanin Human IgG Labeling Kit , 25 <strong>labeling</strong>s Human 1 kit Z-25451<br />

Zenon® R-Phycoerythrin Human IgG Labeling Kit , 25 <strong>labeling</strong>s Human 1 kit Z-25455<br />

Zenon® Tricolor Human IgG Labeling Kit #1,<br />

for green, orange, <strong>and</strong> deep red fluorescence imaging, 3 × 10 <strong>labeling</strong>s<br />

Human 1 kit Z-25460<br />

Zenon® Tricolor Human IgG Labeling Kit #2,<br />

for blue, green, <strong>and</strong> red fluorescence imaging, 3 × 10 <strong>labeling</strong>s<br />

Human 1 kit Z-25470<br />

Zenon® Alexa Fluor® 350 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25000<br />

Zenon® Alexa Fluor® 350 Mouse IgG2a Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25100<br />

Zenon® Alexa Fluor® 350 Mouse IgG2b Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25200<br />

Zenon® Alexa Fluor® 405 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25013<br />

Zenon® Alexa Fluor® 405 Mouse IgG2a Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25113<br />

Zenon® Alexa Fluor® 405 Mouse IgG2b Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25213<br />

Zenon® Alexa Fluor® 430 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25001<br />

Zenon® Alexa Fluor® 488 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25002<br />

Zenon® Alexa Fluor® 488 Mouse IgG2a Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25102<br />

Zenon® Alexa Fluor® 488 Mouse IgG2b Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25202<br />

Zenon® Alexa Fluor® 532 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25003<br />

Zenon® Alexa Fluor® 546 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25004<br />

Zenon® Alexa Fluor® 546 Mouse IgG2a Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25104<br />

Zenon® Alexa Fluor® 546 Mouse IgG2b Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25204<br />

Zenon® Alexa Fluor® 555 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25005<br />

Zenon® Alexa Fluor® 555 Mouse IgG2a Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25105<br />

Zenon® Alexa Fluor® 555 Mouse IgG2b Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25205<br />

Zenon® Alexa Fluor® 568 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25006<br />

Zenon® Alexa Fluor® 568 Mouse IgG2a Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25106<br />

Zenon® Alexa Fluor® 568 Mouse IgG2b Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25206<br />

Zenon® Alexa Fluor® 594 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25007<br />

Zenon® Alexa Fluor® 594 Mouse IgG2a Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25107<br />

Zenon® Alexa Fluor® 594 Mouse IgG2b Labeling Kit , 50 <strong>labeling</strong>s Mouse 1 kit Z-25207


Product Species Quantity Cat.<br />

Zenon® Technology products (contd.)<br />

no.<br />

Zenon® Alexa Fluor® 610–R-Phycoerythrin Mouse IgG1 Labeling Kit, 10<br />

<strong>labeling</strong>s<br />

Mouse 1 kit Z-25020<br />

Zenon® Alexa Fluor® 647 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25008<br />

Zenon® Alexa Fluor® 647 Mouse IgG2a Labeling Kit , 50 <strong>labeling</strong>s Mouse 1 kit Z-25108<br />

Zenon® Alexa Fluor® 647 Mouse IgG2b Labeling Kit , 50 <strong>labeling</strong>s Mouse 1 kit Z-25208<br />

Zenon® Alexa Fluor® 647–R-Phycoerythrin Mouse IgG1 Labeling Kit, 10<br />

<strong>labeling</strong>s<br />

Mouse 1 kit Z-25021<br />

Zenon® Alexa Fluor® 647—R-Phycoerythrin Mouse IgG2a Labeling Kit, 10<br />

<strong>labeling</strong>s<br />

Mouse 1 kit Z-25121<br />

Zenon® Alexa Fluor® 647—R-Phycoerythrin Mouse IgG2b Labeling Kit , 10<br />

<strong>labeling</strong>s<br />

Mouse 1 kit Z-25221<br />

Zenon® Alexa Fluor® 660 Mouse IgG1 Labeling Kit , 50 <strong>labeling</strong>s Mouse 1 kit Z-25009<br />

Zenon® Alexa Fluor® 680 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25010<br />

Zenon® Alexa Fluor® 680 Mouse IgG2a Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25110<br />

Zenon® Alexa Fluor® 680 Mouse IgG2b Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25210<br />

Zenon® Alexa Fluor® 680—R-Phycoerythrin Mouse IgG1 Labeling Kit, 10<br />

<strong>labeling</strong>s<br />

Mouse 1 kit Z-25022<br />

Zenon® Alexa Fluor® 700 Mouse IgG1 Labeling Kit, 50 <strong>labeling</strong>s Mouse 1 kit Z-25011<br />

Zenon® Alexa Fluor® 700—Allophycocyanin Mouse IgG1 Labeling Kit, 10<br />

<strong>labeling</strong>s<br />

Mouse 1 kit Z-25030<br />

Zenon® Alexa Fluor® 750—Allophycocyanin Mouse IgG1 Labeling Kit, 10<br />

<strong>labeling</strong>s<br />

Mouse 1 kit Z-25031<br />

Zenon® Alexa Fluor® 488 Mouse IgG1 Labeling Kit, enhanced with TSA<br />

technology, 25 <strong>labeling</strong>s<br />

Mouse 1 kit Z-25090<br />

Zenon® Alexa Fluor® 568 Mouse IgG1 Labeling Kit, enhanced with TSA<br />

technology, 25 <strong>labeling</strong>s<br />

Mouse 1 kit Z-25091<br />

Zenon® Allophycocyanin Mouse IgG1 Labeling Kit, 25 <strong>labeling</strong>s Mouse 1 kit Z-25051<br />

Zenon® Allophycocyanin Mouse IgG2a Labeling Kit, 25 <strong>labeling</strong>s Mouse 1 kit Z-25151<br />

Zenon® Allophycocyanin Mouse IgG2b Labeling Kit, 25 <strong>labeling</strong>s Mouse 1 kit Z-25251<br />

Zenon® R-Phycoerythrin Mouse IgG1 Labeling Kit, 25 <strong>labeling</strong>s Mouse 1 kit Z-25055<br />

Zenon® R-Phycoerythrin Mouse IgG2a Labeling Kit, 25 <strong>labeling</strong>s Mouse 1 kit Z-25155<br />

Zenon® R-Phycoerythrin Mouse IgG2b Labeling Kit, 25 <strong>labeling</strong>s Mouse 1 kit Z-25255<br />

Zenon® Tricolor Mouse IgG1 Labeling Kit #1, for green, orange, <strong>and</strong> deep red<br />

fluorescence imaging, 3 × 10 <strong>labeling</strong>s<br />

Mouse 1 kit Z-25060<br />

Zenon® Tricolor Mouse IgG1 Labeling Kit #2, for blue, green, <strong>and</strong> red<br />

fluorescence imaging, 3 × 10 <strong>labeling</strong>s<br />

Mouse 1 kit Z-25070<br />

Zenon® Tricolor Mouse IgG1 Labeling Kit #3, for flow cytometry, 488 nm<br />

excitation, 3 × 10 <strong>labeling</strong>s<br />

Mouse 1 kit Z-25080<br />

Zenon® Tricolor Mouse IgG2a Labeling Kit #1, for green, orange, <strong>and</strong> deep red<br />

fluorescence imaging, 3 × 10 <strong>labeling</strong>s<br />

Mouse 1 kit Z-25160<br />

Zenon® Tricolor Mouse IgG2a Labeling Kit #2, for blue, green, <strong>and</strong> red<br />

fluorescence imaging, 3 × 10 <strong>labeling</strong>s<br />

Mouse 1 kit Z-25170<br />

Zenon® Tricolor Mouse IgG2a Labeling Kit #3, for flow cytometry, 488 nm<br />

excitation, 3 × 10 <strong>labeling</strong>s<br />

Mouse 1 kit Z-25180<br />

Zenon® Tricolor Mouse IgG2b Labeling Kit #1, for green, orange, <strong>and</strong> deep red<br />

fluorescence imaging, 3 × 10 <strong>labeling</strong>s<br />

Mouse 1 kit Z-25260<br />

Zenon® Tricolor Mouse IgG2b Labeling Kit #2, for blue, green, <strong>and</strong> red<br />

fluorescence imaging, 3 × 10 <strong>labeling</strong>s<br />

Mouse 1 kit Z-25270<br />

Zenon® Tricolor Mouse IgG2b Labeling Kit #3, for flow cytometry, 488 nm<br />

excitation, 3 × 10 <strong>labeling</strong>s<br />

Mouse 1 kit Z-25280<br />

Zenon® Alexa Fluor® 488 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25302<br />

Zenon® Alexa Fluor® 350 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25300<br />

Zenon® Alexa Fluor® 405 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25313<br />

Zenon® Alexa Fluor® 430 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25301<br />

Zenon® Alexa Fluor® 532 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25303<br />

Zenon® Alexa Fluor® 546 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25304


Product Species Quantity Cat.<br />

Zenon® Technology products (contd.)<br />

no.<br />

Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25305<br />

Zenon® Alexa Fluor® 568 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25306<br />

Zenon® Alexa Fluor® 594 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25307<br />

Zenon® Alexa Fluor® 647 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25308<br />

Zenon® Alexa Fluor® 680 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25310<br />

Zenon® Alexa Fluor® 700 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25311<br />

Zenon® Alexa Fluor® 750 Rabbit IgG Labeling Kit, 50 <strong>labeling</strong>s Rabbit 1 kit Z-25312<br />

Zenon® Allophycocyanin Rabbit IgG Labeling Kit, 25 <strong>labeling</strong>s Rabbit 1 kit Z-25351<br />

Zenon® R-Phycoerythrin Rabbit IgG Labeling Kit, 25 <strong>labeling</strong>s Rabbit 1 kit Z-25355<br />

Zenon® Tricolor Rabbit IgG Labeling Kit #1, for green, orange, <strong>and</strong> deep red<br />

Rabbit 1 kit Z-25360<br />

fluorescence imaging, 3 × 10 <strong>labeling</strong>s<br />

Zenon® Tricolor Rabbit IgG Labeling Kit #2, for blue, green, <strong>and</strong> red<br />

fluorescence imaging, 3 × 10 <strong>labeling</strong>s<br />

Rabbit 1 kit Z-25370

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