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IMAGE 2010-11 ISSN: 2229-5658 Vol No: 11 ISSUE No: 4 39<br />

question is how reliable and accurate these<br />

methods are in calibrating the exact WL.<br />

In view of the possible variation<br />

between the radiographic apex, anatomical apex<br />

and apical foramen, this in vitro study was done<br />

to evaluate the accuracy of Raypex5 (VDW,<br />

Munich, Germany) electronic apex locator<br />

(EAL) in determining the RCL in comparison<br />

to conventional radiography and RVG.<br />

Materials and Methods<br />

Forty single rooted anterior teeth<br />

exhibiting normal and mature root condition<br />

were used in this study. For RCL measurement<br />

by conventional radiography the specimens were<br />

directly positioned on a geometrically<br />

standardized support equipped <strong>with</strong> a horizontal<br />

goniometer (Fig. 1). As a result, it was not<br />

necessary to rotate the X-ray tube since the tooth<br />

could be turned to directly select the horizontal<br />

position of the X-ray beam <strong>with</strong> respect to the<br />

specimen. X-ray cone position was standardized<br />

<strong>with</strong> the use of XCP Rinn fixed to the X-ray tube<br />

so that the X-ray film was perpendicular to the<br />

beam at all times (Fig. 1). In order to ensure<br />

centered and reproducible positioning of the<br />

teeth, a coronal reference point was marked by<br />

making ditch cut on labial surface (2x5x2 mm)<br />

and filled it <strong>with</strong> amalgam. All the specimens<br />

were kept at a constant distance of 10 mm from<br />

the X-ray film and 50mm from the X-ray cone<br />

(Fig. 1). Tooth localization was always<br />

referenced to calibrated graph paper, to allow Xray<br />

cone positioning parallel to the paper lines.<br />

After all these standardization, preoperative Xray<br />

images <strong>with</strong> size zero intraoral periapical<br />

film (Kodak, Rochester, NY, USA) were<br />

obtained and developed <strong>with</strong> an automatic<br />

Periomat device (D¸rr <strong>Dental</strong> GmbH & Co. KG,<br />

Bietigheim-Bissingen, Germany).<br />

Preoperative digitized X-ray images<br />

were also taken in the same <strong>approach</strong> but by<br />

replacing a size zero IOPA film <strong>with</strong> a same size<br />

sensor (eva <strong>with</strong> proimage Æ , DENT.X, Elmsford,<br />

NY, USA). On the sensor, 10mm premeasured<br />

metal wire was fixed <strong>with</strong> the help of cello tape<br />

for giving precaliberation measurements of<br />

digitized images. Digital vernier caliper (Sankin,<br />

Mitutoyo Co., Kanagawa, Japan) <strong>with</strong> accuracy<br />

to the nearest 0.01mm was in turn used to obtain<br />

measurements of each specimen from all the<br />

preoperative X-ray images by viewing them on<br />

viewerís box. RVG provided calibrated<br />

measurements between different points in a<br />

given image and the maximum radiovisiographic<br />

length of each sample was recorded by<br />

calibrating the device for each projection.<br />

Maximum preoperative root length of both<br />

conventional and digitized images was recorded<br />

by measuring the distance between the incisal<br />

edge and the radiographic apex.<br />

Standard access preparation was carried<br />

out and the root canals were located.<br />

Measurement of actual root canal length<br />

(ARCL) was calculated by inserting a size 15 Kfile<br />

into the canal until the tip of the file was just<br />

visible at the level of apical foramen under<br />

2.5x-420 magnifying loupes (Galilean loupes Æ ,<br />

Lifecare Medical Equipments Co., Ltd.,<br />

Zhejiang, China). A rubber stopper was then<br />

carefully adjusted to the reference level, file was<br />

removed and the distance between the rubber<br />

stopper and the file tip was measured and<br />

recorded <strong>with</strong> a digital vernier caliper to the<br />

nearest 0.01mm.<br />

Electronic measurement of RCL was<br />

calculated for each specimen using Raypex5<br />

apex locator by four blinded observers. In order<br />

to reproduce clinical conditions involved in the<br />

electronic measurement of root canal length,<br />

each specimen was mounted in alginate 6 . The<br />

relative stiffness of the alginate mould prevented<br />

fluid movement inside the canal that is<br />

responsible for premature electronic reading<br />

registered <strong>with</strong> previous models 7,8 . All<br />

measurements were made <strong>with</strong>in 2 hours of the<br />

model being prepared in order to ensure the<br />

alginate was kept sufficiently humid 9 . The labial<br />

clip of the Apit device was in contact <strong>with</strong> the<br />

alginate at all times (Fig. 2). Each canal was<br />

irrigated <strong>with</strong> 3% sodium hypochlorite and size<br />

15 K-file attached to the instrumental clip was<br />

inserted into the root canal till the red mark was<br />

visible on the screen of apex locator. The red<br />

mark indicated the position of the apical<br />

foramen. Point at which red mark was seen the<br />

rubber stopper was adjusted to the reference<br />

level, file was taken out and its length was<br />

Visit: http://image.idakunnamkulam.com/<br />

The International Journal of <strong>Indian</strong> <strong>Dental</strong> Association, Kunnamkulam Branch. Indexed in Journals Master List of IC TM

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