2001 - Center for Advanced Biotechnology and Medicine - Rutgers ...

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Center for Advanced Biotechnology and Medicine
Edward Arnold
some of the engineered viruses (and soon peptides), alone and in complex with anti-HIV
antibodies. Ultimately we hope to identify three-dimensional correlates of immunogenicity, and
use this information to develop a structural basis for design of more effective human vaccines.
There is every reason to expect that a structure-based approach to vaccine development will
become as important to vaccinology as has structure-based drug design to drug discovery and
development.
In addition to working to develop novel vaccines and chemotherapeutic agents, the laboratory
aims to gain greater insights into the basic molecular processes of living systems. Other projects
currently being pursued in the lab include structural studies of: 1) the human mRNA capping
enzyme and its associated factors (with Dr. Aaron Shatkin at CABM); 2) the TNF-like cytokine
BLyS (B lymphocyte stimulator, with Yuling Li and colleagues at Human Genome Sciences); 3)
and hantavirus proteins (with Dr. Colleen Jonssen at University of New Mexico). The structural
group also collaborates with Dr. Gaetano Montelione (CABM) on crystallography of proteins
targeted by the Northeast Structural Genomics Consortium.
Publications:
Sarafianos, S.G., Das, K., Tantillo, C., Clark, A.D. Jr., Ding, J., Whitcomb, J., Boyer, P.L.,
Hughes, S.H. and Arnold, E. (2001). Crystal structure of HIV-1 reverse transcriptase in
complex with a polypurine tract RNA:DNA. EMBO J. 20: 1449-1461.
Boyer, P.L., Sarafianos, S.G., Arnold, E. and Hughes, S.H. (2001). Selective excision of AZT
by drug-resistant human immunodeficiency virus reverse transcriptase. J. Virol. 75:4832-4842.
Das, K, Xiong, X., Yang, H., Westland, C.E., Gibbs, C.S., Sarafianos, S.G. and Arnold, E.
(2001). Molecular modeling and biochemical characterization reveals the mechanism of
hepatitis B virus polymerase resistance to lamivudine (3TC) and emtricitabine (FTC). J. Virol.
75:4771-4779.
Hsiou, Y., Ding, J., Das, K., Clark, A.D., Jr., Boyer, P.L., Lewi, P., Janssen, P.A.J., Kleim, J.P.
Rösner, M., Hughes, S.H. and Arnold, E. (2001). The Lys103Asn mutation of HIV-1 RT: a
novel mechanism of drug resistance. J. Mol. Biol. 309:437-445.
Tachedjian, G., Orlova, M., Sarafianos, S.G., Arnold, E. and Goff, S.P. (2001). Nonnucleoside
reverse transcriptase inhibitors are chemical inducers of dimerization of the human
immunodeficiency type 1 reverse transcriptase. Proc. Natl. Acad. Sci. USA. 98:7188-7193.
Boyer, P.L., Gao, H.-Q., Clark, P.K., Sarafianos, S.G., Arnold, E. and Hughes, S.H. (2001).
YADD mutants of human immunodeficiency virus type 1 and Moloney murine leukemia virus
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