2001 - Center for Advanced Biotechnology and Medicine - Rutgers ...

on CE. We also found that MT interacts with the nuclear transporter, importin-alpha (Impa). MT
selectively bound and methylated RNA containing 5'-GpppG, and both activities were stimulated
Aaron J. Shatkin
several-fold by Impa. MT/RNA/Impa complexes were dissociated by addition of Imp-beta
(Impb) which also blocked Impa stimulation of RNA cap methylation. RanGTP but not RanGDP
prevented these effects of Impb. The results suggest that, in addition to a linkage between
capping and transcription, mRNA biogenesis and nucleocytoplasmic transport are functionally
connected, a possibility we are exploring further.
Publications:
Shatkin, A.J. (2000). Reovirus translational control. Translational Control, Sonenberg, N.,
Hershey, J.W.B., Mathews, M.B., eds. Cold Spring Harbor Laboratory Press, 2nd edition, 915-
932.
Furuichi, Y. and Shatkin, A.J. (2000). Viral and Cellular mRNA Capping: Past and prospects.
Adv. in Vir. Res, 55:135-184.
Shatkin, A.J. and Manley, J.L. (2000). The ends of the affair: Capping and polyadenylation.
Nature Structural Biology. 7:838-842.
Wen, Y. and Shatkin, A.J. (2000). Cap methyltransferase selective binding and methylation of
GpppG-RNA are stimulated by importin-α. Genes and Development. 14:2944-2949.
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