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Ensayos de metabolismo microbiano en suelo: actividad ...

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302<br />

<strong>Ensayos</strong> para <strong>suelo</strong>s<br />

Tabla 19.1. Condiciones recom<strong>en</strong>dadas para las pruebas <strong>de</strong> la tasa <strong>de</strong> mineralización<br />

<strong>de</strong>l nitróg<strong>en</strong>o <strong>en</strong> <strong>suelo</strong> (continúa)<br />

Almac<strong>en</strong>ami<strong>en</strong>to <strong>de</strong> los extractos antes Congelación a 5 °C<br />

<strong>de</strong> su análisis<br />

Efectos medidos Conc<strong>en</strong>traciones <strong>de</strong> amonio, nitratos y nitritos<br />

Resultado final µg <strong>de</strong>l Nitróg<strong>en</strong>o mineralizado por gramo <strong>de</strong><br />

<strong>suelo</strong> por día<br />

Control negativo Suelos sin tratami<strong>en</strong>to<br />

Bibliografía<br />

Alef, K. y D. Kleiner. 1986a. Arginine ammonification, a simple method to estimate<br />

microbial activity pot<strong>en</strong>tials in soils. Soil Biology and Biochemistry 18: 233-135.<br />

———. 1986b. Arginine ammonification in soils samples. En: The application of <strong>en</strong>zymatic<br />

and microbiological methods in soil analysis. Veroff Landwirtsch-Chem.<br />

Bun<strong>de</strong>sanstalt Linz/Donau 18, 163-168.<br />

Casida, L. E. Jr., 1977. Microbial metabolic activity in soil as measured by <strong>de</strong>hydrog<strong>en</strong>ase<br />

<strong>de</strong>terminations. Applied and Environm<strong>en</strong>tal Microbiology 34: 630-636.<br />

Chan<strong>de</strong>r, K., y P.C. Brookes. 1991. Is the <strong>de</strong>hydrog<strong>en</strong>ase activity assay invalid as a<br />

method to estimate microbial activity in copper-contaminated soils? Soil Biology<br />

and Biochemistry 23: 909-915.<br />

Cochran V. L., L. F. Elliot y C. E. Lewis. 1989. Soil microbial biomass and <strong>en</strong>zyme<br />

activity in subartic agricultural and forest soils. Biology and Fertility of Soils 7:<br />

283-288.<br />

García C., T. Hernán<strong>de</strong>z y F. Costa. 1997. Pot<strong>en</strong>tial use of <strong>de</strong>hydorg<strong>en</strong>ase activity as<br />

an in<strong>de</strong>x of microbial activity in <strong>de</strong>gra<strong>de</strong>d soils. Comunication. Soil Sci<strong>en</strong>ce and<br />

Plant Analysis 28: 123-134.<br />

Greaves M. P., N. J. Poole, K. H. Domsch, G. Jagnow y W. Verstraete. 1980. Recomm<strong>en</strong><strong>de</strong>d<br />

test for assessing the si<strong>de</strong> effect of pestici<strong>de</strong>s on soil microflora. Technical<br />

Report Research Council, Weed Research Organization. No 59: 1-15.<br />

Haynes R.J., y K.M. Goh. 1978. Ammonium and nitrate nutrition of plants. Biological<br />

Reviews 53: 465-510.<br />

J<strong>en</strong>kinson D. S. y J. N. Ladd. 1981. Microbial biomass in soil: measurem<strong>en</strong>t and turnover.<br />

En: E. A. Paul y J. N. Ladd (eds). Soil Biochemistry. Vol. 5. Marcell Dekker.<br />

New York. Pp. 415-471.<br />

Ke<strong>en</strong>ey D. R., y D. W. Nelson. 1982. Nitrog<strong>en</strong> inorganic forms. En: A. L. Page, R. H.<br />

Miller y D. R. Ke<strong>en</strong>ey (eds). Methods of Soil Analysis. Part 2. Segunda edición.<br />

American Society of Agronomy, Madison, Washington D.C. Pp. 643-698.

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