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Determinación de coliformes: Método del NMP.

Determinación de coliformes: Método del NMP.

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Día 1 (Prueba presuntiva)<br />

1. Preparación <strong>de</strong>l material.<br />

2. Realizar diluciones seridas <strong>de</strong> la muestra <strong>de</strong><br />

agua hasta 10 -3 en agua peptonada.<br />

3. Realiazar 5 pases <strong>de</strong> 1 ml. <strong>de</strong> cada una <strong>de</strong> las<br />

diluciones (10 -1 , 10 -2 , 10 -3 ) a tubos con 9 ml.<br />

<strong>de</strong> caldo Lauril sulfato y campanas Durham.<br />

4. Dejar incubando <strong>de</strong> 24 a 48 hrs. a 35°C.

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