NGS Illumina sequencing: a pratical overview - Centri di Ricerca ...
NGS Illumina sequencing: a pratical overview - Centri di Ricerca ...
NGS Illumina sequencing: a pratical overview - Centri di Ricerca ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
TARGET ENRICHMENT OPTIONS<br />
Custom target<br />
You can choose which region and for which organisms (need a reference) to do<br />
genome capture<br />
Region captured from 200Kb to 32Mbp for Agilent SureSelect system<br />
Region captured from 700Kb to 15Mbp for <strong>Illumina</strong> system<br />
Software to design the probes: <strong>Illumina</strong> Design Stu<strong>di</strong>o, Agilent eArray<br />
ONLY human target re<strong>sequencing</strong><br />
Human all exon<br />
Agilent SureSelect all exon 50Mbp= target size about 50 Mbp<br />
<strong>Illumina</strong> TrueSeq exon enrichment kit = target size about 62Mbp<br />
Custom target re<strong>sequencing</strong><br />
Amplicon Seq (<strong>Illumina</strong>): 12 to 96 Kbp<br />
Halopex (Agilent): until 1Mbp
Change language