Analisi genetica del ciclo cellulare - Università degli Studi della Tuscia

Limited biochemistry confirmed
that Cdc2 is a Protein Kinase
Functional characterization:
- Cdc2 immunoprecipitates have kinase activity
- this kinase activity peaks at G2/M
- the kinase activity from cdc2 ts lysates
disappears at high temperature
Studies of genetic interactions suggested, however,
that Cdc2 did not function alone: Cdc13 mutations
had just the same phenotype as Cdc2, and mild
alleles of the two genes were additive, an example
of a “synthetic genetic interaction.”
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