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Anti TRANSGLUTAMINASI IgG EIA WELL R REF - Radim S.p.A.

Anti TRANSGLUTAMINASI IgG EIA WELL R REF - Radim S.p.A.

Anti TRANSGLUTAMINASI IgG EIA WELL R REF - Radim S.p.A.

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Prediluizione del campione, Sample predilution: 1/100.<br />

Pozzetti<br />

Wells<br />

Bianco,<br />

Blank<br />

CAL<br />

(1-5)<br />

CTR Campioni,<br />

Samples<br />

Reag<br />

CAL --- 100 µL --- ---<br />

CTR --- --- 100 µL ---<br />

Campioni, Samples --- --- --- 100 µL<br />

DIL 100 µL --- --- ---<br />

− Incubare, Incubate: T.A./R.T. 60±5 min. 1200 rpm<br />

− Aspirare e lavare, Aspirate and wash: 4 x 350 µL.<br />

CONJ 100 µL 100 µL 100 µL 100 µL<br />

− Incubare, Incubate: T.A./R.T. 30±2 min. 1200 rpm<br />

− Aspirare e lavare, Aspirate and wash: 4 x 350 µL.<br />

TMB 100 µL 100 µL 100 µL 100 µL<br />

− Incubare, Incubate: T.A./R.T. 10 min. 1200 rpm<br />

STOP 100 µL 100 µL 100 µL 100 µL<br />

− Leggere, Read: 450-405 nm.<br />

K10TG - <strong>Anti</strong>-Transglutaminasi <strong>IgG</strong> <strong>EIA</strong> <strong>WELL</strong><br />

M412 – Rev.1 – 04/2008 – Pag. 22/24

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