Prediluizione del campione, Sample predilution: 1/100. Pozzetti Wells Bianco, Blank CAL (1-5) CTR Campioni, Samples Reag CAL --- 100 µL --- --- CTR --- --- 100 µL --- Campioni, Samples --- --- --- 100 µL DIL 100 µL --- --- --- − Incubare, Incubate: T.A./R.T. 60±5 min. 1200 rpm − Aspirare e lavare, Aspirate and wash: 4 x 350 µL. CONJ 100 µL 100 µL 100 µL 100 µL − Incubare, Incubate: T.A./R.T. 30±2 min. 1200 rpm − Aspirare e lavare, Aspirate and wash: 4 x 350 µL. TMB 100 µL 100 µL 100 µL 100 µL − Incubare, Incubate: T.A./R.T. 10 min. 1200 rpm STOP 100 µL 100 µL 100 µL 100 µL − Leggere, Read: 450-405 nm. K10TG - <strong>Anti</strong>-Transglutaminasi <strong>IgG</strong> <strong>EIA</strong> <strong>WELL</strong> M412 – Rev.1 – 04/2008 – Pag. 22/24
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