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SCHEMA DEL DOSAGGIO, ASSAY SCHEME<br />
Prediluizione del Campione, Sample predilution: 1/101<br />
Provette<br />
Tubes<br />
A.Tot<br />
Tot Act<br />
(T)<br />
CAL<br />
(0-5)<br />
<strong>CT</strong>R Campioni,<br />
Samples<br />
Reag.<br />
CAL (0 - 5) ---- 100 Pl ---- ----<br />
<strong>CT</strong>R 1 ---- ---- 100 Pl ----<br />
<strong>CT</strong>R 2 ---- ---- 100 Pl ----<br />
Sample 100 Pl<br />
− Agitare e incubare, Mix and incubate: 2h T.A. su agitatore orbitale (150 rpm).<br />
− Aspirare e lavare, Aspirate and wash: 2 x 1 ml.<br />
CONJ 100 Pl 100 Pl 100 Pl 100 Pl<br />
− Agitare e incubare / Mix and incubate<br />
A: 90 minuti T.A./R.T. (150 rpm-U/min)<br />
O, or<br />
B: 18-24 h. a T.A./R.T.<br />
− Aspirare e lavare, Aspirate and wash: 2 x 1 ml.<br />
− Leggere, Read.<br />
KT8<strong>CT</strong> – <strong>TgAb</strong> irma <strong>CT</strong><br />
M91 – Rev.01 – 09/2004 – Pag. 23/24
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