vegetation was studied in summer 2012 using a transect method, estimating macrophyte speciescomposition and occurrence (on a five – point scale). In Dienvidu Garezers Lake and Vidējais GarezersLake 21 transects were set, but in ZiemeĜu Garezers Lake – 11 transects. To estimate biologicaldiversity of macrophyte vegetation species diversity indices were used. To assess ecological quality ofthe lakes, Polish Ecological State Macrophyte Index (ESMI), British Lake Macrophyte Nutrient Index(LMNI), and for Latvia adapted Estonian transect method with combination of phytolittoral mappingmethod, based on estimating abundance of macrophyte growth forms were used. Together in GarezeriLakes were recognized 36 macrophyte taxa. Highest number of taxa (27) was appointed in DienviduGarezers Lake. Species with highest occurrence in all lakes were Nuphar lutea, Phragmites australis,Carex acuta, Comarium palustre, Lythrum salicaria. Only in Dienvidu Garezers Lake was identified twoprotected and rare species - Lobelia dortmannaand Isoetes lacustris. In the Garezeri lakes helophytesdominate in amount of species and total occurrence. Highest number of species and total macrophyteoccurrence was estimated in the most shallow depth zone (0 – 1 m). Highest biological diversity is inZiemeĜu Garezers Lake, where is the highest Shannon index (H’ = 0,913) and the lowest Simpson’sindex (D = 0,135) values. In all three lakes existent species diversity and distribution equality is closeto maximum possible. According to ESMI and for Latvia adapted Estonian method ecological qualityof Garezeri Lakes is good. LMNI shows good ecological quality in Dienvidu and Vidējais GarezersLake, but moderate ecological quality in ZiemeĜu Garezers Lake.Vija Frīda<strong>Daugavpils</strong> Universitys Parades street 1, <strong>Daugavpils</strong>,Latvia, vija.frida@gmail.comDENDROLOGY ANALYSIS IN NATURE RESERVE“PILSKALNES SIGULDIĥA”Nature Reserve „Pilskalnes SiguldiĦa” is a protected area with high biological diversity. The NatureReserve was created in 1999 and it is included in the Natura 2000 network, but its Natural resourcemanagement plan and Individual protection and use regulations have not been approved yet.In order to create these documents it is necessary to investigate natural values in the Nature Reserve.The aim of this paper is to make inventories of secular trees.Thirty-three secular trees of national significance and local significance have been investigated:circumference, height, crown projection, condition.As the results of the research nine secular oaks (circumference ≥4m), one secular spruce(circumference ≥3m) and one secular ash (circumference ≥3,5m) of national significance have beendiscovered. Only one of the investigated secular trees has already been under state protection – Senčusecular oak.AcknowledgementsResearch reported in this article was supported by the ESF project No.2011/0018/1DP/1.1.2.1.1/11/IPIA/VIAA/010 “Support of master’s studies in <strong>Daugavpils</strong>University”.~ 12 ~
ĂENĒTIKA UN BIOTEHNOLOĂIJAS≈GENETICS AND BIOTEHNOLOGYLina Zybartaite, Rasa Janulioniene, Ramunas Vilcinskas, Judita Zukauskien÷,Algimantas Paulauskas, Eugenija KupcinskieneVytautas Magnus University, Vileikos street 8, Kaunas, Lithuania, dek@gmf.vdu.ltAPPLICABILITY OF RANDOMLY AMPLIFIED POLYMORPHICDNA MARKERS TO ANALYZE POPULATIONS OF IMPATIENS SPPKey words: small balsam, Himalayan balsam, touch-me-not, RAPD, alien species, invasive species.Five out of 1000 Balsaminaceae species are present in Europe. Among them only Impatiens noli-tangere isnative to this continent,while I. glandulifera and I. parviflora were introduced to Europe in 19 th centuryand nowadays as aliens are widely spread, as an escape, I. balfourii is not often met and I. balsamina isknown only as introduced and grown ornamental plant. With exception of I. balfourii the same is truefor Lithuania. Valuable information might be obtained comparing adaptation level and variability ofalien species to native one. The objective of our research was to select randomly amplifiedpolymorphic DNA (RAPD) markers appropriate for genetic assessment of Impatiens spp. populationsgrowing in Lithuania. For this purpose ten nucleotide length 30 RAPD primers were tested. Eight themost informative primers (222, 250, 269, 340, 474, 516, OPA-20, OPD-20) generated polymorphic DNAbands and were suitable for all three species. Polymorphic chain reaction and DNA amplificationconditions were the same for all species. Annealing temperature for primer OPA-20 was 32 °C, forprimers 222, 250, 269, 340, 474, OPD-20 were 34 °C and for primer 516 it was 36 °C. The reactionproducts were fractionated by electrophoresis in 1.5 % agarose gel stained with ethidium bromide.The length of DNA bands were estimated according to the 100 bp gene ruler. Examination of 20populations of I. glandulifera, 21 population of I. parviflora and 15populations of I. noli-tangere hasshown that the longest DNA bands were generated by OPA-20 primer for all species (respectively,3100, 2900 and 2700 base pairs). For I. glandulifera, the shortest DNA bands (1700 base pairs) wereobtained by OPD-20 primer, for I. parviflora (1400 base pairs) by 516 primer, for I.noli-tangere (1800base pairs) also by 516 primer. For some populations of I. parviflora primers OPA-20, OPD-20, 250, 340,516 did not generate any DNA band, for I. glandulifera populations such primers were OPA-20 and 250and for I. noli-tangere it was primer 516. The highest number of DNA bands (30 bands), in I.glandulifera were generated by primer 516, in I. parviflora populations such primer was 222 (43 bands)and in I.noli-tangere populations also by primer 222 (40 bands). Obtained results revealed that chosenRAPD markers are valuable for evaluation of molecular diversity between Impatiens spp. populations.Kokina Inese, JermaĜonoka Marija, Valaine Kristīna, RubeniĦa Ilze, Gavarāne Inese<strong>Daugavpils</strong> University, Vienibas street 13, <strong>Daugavpils</strong>, Latvia, inese.kokina@du.lv, inese.gavarane@biology.lvDETECTION OF SOMACLONAL VARIATION IN FLAX (LINUMUSITATISSIMUM L.) CALLI AND REGENERANTS OBTAINED ONMEDIUM SUPPLEMENTED BY CARBON NANOPARTICLESFlax is the most known crop in the world, a major oil and fiber source (Salaj et al., 2005). The aim ofour study was to develope a method for detection of somaclonal variation in flax donor plants, calliand regenerants. Stem segments of Latvian origin flax accession ‘Blue di Riga’ were used for calliformation. For further cultivation, calli were transferred on the medium (MS with 1 mg/l of 2,4 D and~ 13 ~
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Irēna Mihailova 1 , Vjačeslavs Ge
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MATEMĀTIKA≈MATHEMATICSSalvis Spe
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Anita KiričukaDaugavpils Universit
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Jūlija SivahoDaugavpils Universit
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elektronisko sistēmu arī vieglo t
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Evita VingreDaugavpils Universitāt
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avoids the harmonization of the pro
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kādā veidā liedzis iespēju nov
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kritēriju valodas paveidu lietoša
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Marta StepnowskaThe John Paul II Ca
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Nikolajs JefimovsDaugavpils Univers
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Александр БайковДа
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Pēteris DedelisIeslodzījuma vietu
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Jurijs Mašošins, Jelena GruznovaD
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Līga MirlinaBiznesa Augstskola “
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Anastasija IvanovaDaugavpils Univer
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Jānis KudiĦšDaugavpils Universit
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izmaiĦas. Strukturālās izmaiĦas
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Diāna Vanaga, Ilona KuĦickaDaugav
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Reăionālās attīstības jautāju
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Līga Vovere¹, Veronika Buăina²
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Pāvels LindemanisRīgas Tehniskā
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Novērots, ka vidējā svērtā atz
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group (equal number of males and fe
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Pētījumā piedalījās 40 pedagog
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Vineta KornejevaDaugavpils Universi
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Marija VasalauskieneDaugavpils Univ
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PEDAGOĂIJA: PĒTĪJUMI UN INOVĀCI
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Aija StudenteRīgas Pedagoăijas un
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Terēza LandraUniversity of Latvia,
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sadarbības organizēšanā. Šādu
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competencies). As the result, the a
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Aivars BulisDaugavpils Universitāt
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gars, ir svarīgi, lai viĦam nebū
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Jevgenija Mackeviča, Irina Kazakev
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Zane ButlereDaugavpils University,
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Natalja PrilenskaDaugavpils Univers
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tradīcija tiek pārĦemta transfor
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dominante ir aktualizēts hronoloă
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Savās pasakās Mērike izmanto vā
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Inesa AntonovaDaugavpils University
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Дина АзереДаугавпи
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Following Celtic and Irish folklore
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Ilona ěahaDaugavpils University, V
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Vineta RūtenbergaLatvia University
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Inesa AntonovaDaugavpils University
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этого страдавший б
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Daiga Vasiljeva, Ilze VolonteDaugav
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Agnese PavlovaDaugavpils Universit
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Ilmārs DirveiksDaugavpils Universi
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kas attiecas uz sociāliem, politis
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mūzikas kultūras tradīciju, sist
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Raksta noformēšanas paraugs:KVALI
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ZVIEDRU FILOLOĂIJA: PĒTĪJUMI, AK