EDIÇÃO 41 RBCIAMB

Formulation of sediments with characteristics of tropical lotic systems for use in ecotoxicological assays
on the natural sediment characteristics, as well as the
commercial availability and ease of use in the laboratory.
Further studies will be necessary to evaluate the
interaction of different types of organic matter with
contaminants, together with their influence on the
bioavailability of toxic pollutants to benthic organisms.
Tests of survival, growth, and reproduction of Hyalella azteca using sediment formulations
Results of the ecotoxicological analyses provided support
for the results obtained in chemical analyses of
water for formulations 4 and 5. All the exposed organisms
survived until the end of the experiments, and
the values obtained for growth parameters were very
similar, even in the comparison of the two formulations.
The average weight and length values (using 10
replicates for each formulation) are shown in Table 3.
The minimum and maximum values obtained for the
lengths of organisms were 2.07 and 2.67 mm respectively
for formulation 4, and 2.09 and 3.41 mm respectively
for 5.
Kruskal-Wallis analysis of variance test showed that for
the length parameter there were no significant differences
between formulations 4 and 5 and their control
(p > 0.05). Additionally, for the average weight of the
organisms, application of Fisher’s exact test revealed no
significant differences between the test formulations
and their corresponding controls (p > 0.05 in both cases).
The reproduction parameter also showed considerable
similarity between the treatments using the different
formulations. The average numbers of offspring produced
per couple at the end of two weeks (using five
replicates for each formulation) are listed in Table 4.
The smallest number was three offsprings (formulation
4B), and the greatest number was 11 offsprings (formulations
4C, 5A, and 5D). These results are similar
to those of Fracácio et al. (2011), who found about 7 to
11 offsprings per couple aging between 30 to 42 days.
Kruskal-Wallis analysis of variance test resulted in p >
0.05 for formulations 4 and 5 and between both formulations,
therefore they suggest that there were no
significant differences between the varied treatments
in terms of numbers of produced offspring.
Table 3 – Physiological parameters of the organisms after 10 days of contact with sediment formulations.
Formulation Average weight (µg) Average length (mm)
Control 4 0.123 2.300 ± 0.081
4A 0.132 2.307 ± 0.061
4B 0.136 2.276 ± 0.104
4C 0.141 2.386 ± 0.146
4D 0.128 2.307 ± 0.147
Control 5 0.138 2.280 ± 0.093
5A 0.155 2.300 ± 0.084
5B 0.143 2.438 ± 0.245
5C 0.139 2.251 ± 0.118
5D 0.132 2.266 ± 0.086
Table 4 – Total numbers of offsprings produced per couple after 14 days of contact with sediment formulations.
Formulation Control 4 4A 4B 4C 4D Control 5 5A 5B 5C 5D
Average offspring
per couple
8.0 ± 1.0 8.0 ± 1.0 7.6 ± 2.8 7.2 ± 2.3 7.0 ± 1.2 8.0 ± 1.0 8.6 ± 1.5 7.6 ± 1.7 7.6 ± 1.1 8.4 ± 1.5
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RBCIAMB | n.41 | set 2016 | 1-11