POE - INDUSTRIA PETROCHIMICA DIN ROMANIA – - ICECHIM

NANOSTRUCTURED PEDOT-METALLO-PORPHYRIN CATALYTIC PLATFORM TO
DETECT PEROXYNITRITE
Serban F. Peteu, Mekki Bayachou
Department of Chemistry, Cleveland State University, 2121 Euclid Ave,
Cleveland, Ohio 44115, United States of America
Peroxynitrite (O=N—O—O - ) is generated by nitric oxide’s reaction with superoxide anion in
cell and organelles. It is a powerful oxidizing and nitrating agent, and his presence was correlated with
the pathogenesis of many degenerative conditions such as Alzeimer’s, Parkinson’s, cancer or AIDS.
Currently, peroxynityrite is detected by chemiluminescence, fluorescence, LC-MS or with
electrochemically modified carbon microfibers
This talk outlines efforts to fabricate and test metallo-porphirin-modified carbon fiber
microelectrodes (CFEs) for peroxynitrite’s quantification. The analyte peroxynitrite was synthesized in
house fresh every time, by the two-phase displacement reaction. The investigations included: field
emission scanning electron microscopy (FESEM) characterization, cyclic voltammetry and time based
amperometry. The sensitivity, detection limit, response time and selectivity are characterized.
Moreover, the electroactive polymer poly(3,4-ethylenedioxythiophene) or PEDOT was
employed to improve both the detection limit and sensitivity. Indeed, this talk will show that the
sensitivity for the hemin-PEDOT modified CFE was 13 pA/nM. That is 130 times higher than bare
CFE, or 50 times higher than hemin-modified CFE. Also, the detection limit for the hemin-PEDOT
CFE was 200 nM for a 3/1 response/noise ratio.
Comunicare orala
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