Rening av proteiner: hur och varför?
Rening av proteiner: hur och varför?
Rening av proteiner: hur och varför?
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Mass spektrometri ger oss identitet på många fläckar....<br />
kDa<br />
100<br />
70<br />
50<br />
30<br />
20<br />
Hsp82<br />
Hsc82<br />
Ssa2<br />
Sse1<br />
Vma1 Sti1<br />
Met6<br />
Ssa1 Ssb1/2 Pab1<br />
Hsp60<br />
Ctt1<br />
Dak1 Ald6<br />
Pdc1<br />
Hxk2<br />
Thr4 Pdc5<br />
Pyk1<br />
Pgi1<br />
Gdh1<br />
Atp2<br />
Ald3<br />
Sam1<br />
Arg1<br />
Lys9 Eno2 Met17 Eno1 Smh2<br />
Tef1/2<br />
Gpd1<br />
Sam2<br />
Tuf1<br />
Gln1<br />
Pgk1<br />
Yil041w Act1<br />
Adh1<br />
Psa1<br />
Fba1<br />
Ilv5<br />
Rnr4<br />
Yjr105w<br />
Tal1<br />
Tdh3<br />
Ipp1<br />
Thi5/11/12, Ydl244w<br />
RplA0<br />
Pdb2<br />
Mol1<br />
Tdh2<br />
Efb1<br />
Bmh2<br />
Yst1 Bmh1<br />
Ymr116c<br />
Tdh1<br />
Gpp1<br />
Bgl2 Yst2<br />
Gpp2<br />
Ykl056c<br />
Ylr109w<br />
Tsa1<br />
Hsp26<br />
Adk1<br />
Sod1<br />
Tpi1<br />
5.0 6.0 7.0 8.0 9.0<br />
pI (pH)<br />
Gpm1<br />
Proteins in blue were recently identified by MS-MALDI ,<br />
Proteins marked in red were identified by sequencing.<br />
bildanalys används för att<br />
detektera <strong>och</strong> katalogisera<br />
punkterna.<br />
Idealt ska <strong>proteiner</strong> vandra<br />
som normalfördelade toppar.<br />
Joakim Norbeck vt-08<br />
Joakim Norbeck vt-08<br />
24
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