Processing Catalog - EMD Chemicals

Processing 

Products and Services 

Advancing Your Life Sciences – 

From Discovery to Launch TM

Contents 

page 4 

6 

8 

10 

48 

62 

12 

14 

16 

18 

19 

19 

20 

22 

22 

23 

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25 

26 

26 

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62 

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63 

65 

71 

Preface 

Advancing Your Life Sciences – From Discovery to Launch TM 

Research and Development 

Processing for Life Sciences – What we Offer 

Amino Acids for Processing 

Biological Buffers for Processing 

Benzonase® endonuclease – the Smart Solution for DNA Removal 

Biochromatography 

New Robust Tentacle Media 

Ion Exchange Chromatography on Fractogel® EMD Tentacle Resins 

Advantages of the Tentacle Ion Exchangers 

Fractogel® EMD Tentacle Phases for Hydrophobic Interaction Chromatography 

Metal Chelate Affinity Chromatography on Fractogel® EMD Chelate 

Thiophilic Adsorption Chromatography on Fractogel® EMD TA 

Molecular Size Exclusion Chromatography on Fractogel® EMD BioSEC (S) 

Regulatory Support Files 

Process and Production Chromatography 

Standardized Silica Gels 

LiChroprep® 

LiChrospher® 

Microcrystalline Cellulose 

Magnesium Silicates – Florisil® 

Standardized Silica Gels – Modified 

PharmPrep®CC 

Aluminium Oxide for Preparative Chromatography 

Preparative Liquid Chromatography Columns 

Lobar®-Ready-to-Use Glass Columns for Medium-Pressure Chromatography 

Hibar®-Ready-to-Use Pre-Packed Columns (250 – 25 mm) 

Customized Preparative Columns (25 and 50 mm, i.d.) of Higher Quality 

Column Packing Stands NW25, NW50 and NW100: 

Innovative Column Packing Technology 

Packing and Unpacking the Packing Stands 

Chromolith® Prep Si 

Contract Separation 

Biochemicals for Bioprocessing 

Enzymes for Bioprocessing 

Product Finder 

Ordering Information 

Product Catalogue 

Appendix 

Abbreviations 

Hazard Symbols and their Meanings 

Safety in the Laboratory 

Risk and Safety Information 

Poster – Safety in the Laboratory, Patent and Licence Information, 

Trademark Information, Disclaimer 

3

Preface 

Chemical testing 

Todays requirements as applied to manufacturers of 

pharmaceuticals are higher than ever before. Safety of 

course has absolute priority; however, the shortening 

of development time required for the research and development 

process, the consequent reduction of costs 

and the lengthening of the time during which the 

developed drug can be marketed are also important. 

Merck is highly involved as a partner in the pharmaceutical 

industry and accompanies and supports the 

efforts of our customers during the entire drug development 

process. The materials available from our 

LIFE SCIENCE PRODUCTS Division represent a comprehensive 

range of applications extending from the 

research laboratory to manufacture. 

From the beginning of the development process 

through scale-up and on to production, we can provide 

our customers with the necessary help and support for 

chemical synthesis and biotechnological processes in 

the form of meaningful cooperation. 

We fully comply with all relevant cGMP requirements 

for production, analysis and packaging; this is constantly 

demonstrated within the scope of customer audits and 

FDA inspections. 

Our customer-oriented product offerings of advanced 

separation media for many types of process recovery 

operations is complemented by a comprehensive range 

of production-focused biochemicals. A selection of 

such products and supportive information is reproduced 

in this brochure. However, should the product 

you require not be included, please do not hesitate to 

contact us; we will be happy to help. 

The name Merck stands for product diversity, competence, 

flexibility, quality, safety and information. 

Why not utilize this potential to the full? 

Dr. Walter Johannssen 

Senior Manager 

Life Science Products / BPC Processing 

5

6 

Advancing Your Life Sciences – 

From Discovery to Launch TM 

As a leading global pharmaceutical company, 

Merck KGaA has vast experience in drug 

research and production. This experience also 

drives the high quality safety standards and 

innovations of our Life Science Products 

Division. 

Developing new products requires not only 

innovation but also attention to the needs of 

customers. We at Merck KGaA focus on close 

interaction with our customers along the 

complete pharmaceutical drug development 

chain to anticipate future requirements as 

early and as thoroughly as possible. 

Our customer involvement starts very early in 

the drug development process at the level of 

drug discovery and screening for which we 

offer highly innovative reagents for combinatorial 

chemistry, peptide synthesis, protein 

expression and many other key research 

technologies. 

Following our customers through the drug 

development process to manufacturing, we 

are a responsive and reliable partner for 

Custom Synthesis & Services requirements, 

as well as process recovery operations. 

Finally, we are intimately familiar with the 

challenges new chemical entities (NCEs) and 

new biological entities (NBEs) present for a 

formulator. Our range of unique excipients 

enables you to develop highly patientcompliant 

and innovative dosage forms. 

Product safety is one of the most important 

aspects of the development and production 

of pharmaceutical drugs. 

At Merck KGaA, we know. 

We maintain a state-of-the art quality 

assurance system and provide extensive 

regulatory affairs support. All Merck products 

are documented as prescribed by the leading 

pharmacopoeias in Europe, the US, Asia and 

beyond… 

Our cGMP production facilities reflect the 

latest knowledge in pharmaceutical production. 

Experience and know-how gained 

from our own pharmaceutical manufacturing 

automatically flows into the raw materials 

we supply to others. The wide range of 

products on offer means that our customers 

can obtain all of their requirements for the 

entire production process from one competent 

source. 

The basis for this is the long tradition of 

“Merck Quality”. This in turn is based on 

comprehensive and stringent product 

analysis that certifies “Merck Quality” and 

hence the quality of the customer’s product. 

“Merck Quality” has been known for 

centuries. We strive daily to further advance 

your life sciences – from discovery to launch.

Life Sciences Products 

Research 

Bioscience 

Process 

Development 

Reagents, kits and services 

for disease related research. 

Preclinical 

Trials 

Clinical 

Phases 

Market 

Launch & 

Production 

Processing 

Products and services for fermentation and recovery 

of new chemical and biological entities. 

Custom Synsthesis Services 

Custom synthesis of advanced intermediates 

and active pharmaceutical ingredients. 

Formulation 

Products and services for formulation 

of the final dosage form. 

LSP provides a unique combination of products and services. 

7

8 

Research and 

Development 

Merck and its affiliate EMD/Merck Biosciences provide 

innovative products and solutions to the life science research 

community. 

Merck is focussed on sample preparation and separation in 

protein research and nucleic acid purification. Our product line 

extends from easy-to-use and reproducible reagent kits for 

sample preparation in protein research to unique materials for 

solid phase separation of peptides, proteins or nucleic acids and 

includes the extremely uniform Estapor® microspheres, polymer 

and silica-based particles for the application in diagnostics, life 

sciences and biotechnology. 

Incidentally, Merck KGaA is the world's largest manufacturer of 

Proteinase K – a key product for molecular biologists. None of 

the results recently obtained in the human genome project 

would be conceivable without Proteinase K. 

Through our affiliate EMD/Merck Biosciences and its brands, 

we offer a broad range of products used worldwide in diseaserelated 

life sciences research. 

Calbiochem® brings quality biochemical and immunological 

reagents with a focus on signal transduction, protein chemistry 

and glycobiology to the Life Science R&D market. 

Novabiochem provides high-purity innovative reagents used 

in Life Science research. Its specific areas of focus are peptide 

synthesis and immobilized reagents. 

Novagen is the gold standard for protein expression and is 

dedicated to developing and commercializing advanced reagents 

for the isolation, expression, analysis and purification of genes 

and their protein products. 

Oncogene offers Life Science research application-tested 

immunology products for apoptosis and cell cycle research, with 

particular focus on cancer, neuroscience, AIDS, Alzheimer’s 

disease and high throughput drug screening.

10 

Processing for Life Sciences – 

Merck Processing for Life Sciences offers a unique combination 

of preparative chromatography resins, both for biopharmaceutical 

and chemical API recovery on a large scale. A wide range of 

biochemicals and additives, which are especially produced to 

fulfill the regulatory requirements for the pharmaceutical 

industry, complements this. 

Chromatography for Processing 

Merck has been active in chromatography for more than 100 years. 

Based on this long tradition of highest quality silica-based resins, 

Merck today is the world’s largest producer of silica gels for chromatographic 

applications. 

When the biopharmaceutical market evolved, it was only natural to 

take this experience into the market of biochromatography. More 

than 15 years’ ago, Merck introduced Fractogel® EMD resins based on 

the innovative tentacle technology. The Fractoprep® EMD resin product 

line recently complemented this product family and confirms 

Merck’s constant strive for innovative chromatography products. This 

recent investment is also a symbol of our long-term commitment to this 

business. 

Biochemicals for Processing 

The focus on the recovery of active pharmaceutical ingredients has 

lead to the development of our broad range of biochemicals and 

additives. The regulatory agencies constantly demand an ever-higher 

level of documentation. Merck has set up an international team consisting 

of regulatory experts, highly qualified scientists and experienced 

project managers to accompany your projects from the research 

phase to clinical trials and finally to the production of the 

approved drug. 

We offer a broad portfolio of amino acids, buffers and enzymes. 

Please ask for our customized packings and special analytics. 

Benzonase® endonculease is the first industrial nuclease for the 

removal of DNA and RNA contaminants. 

More about our product range is contained in this brochure. 

We would be happy to meet you on our newly designed web pages: 

www.merck-lsp.de 

If you need a personal contact by one of our sales specialists, 

please contact us.

What we Offer 

Amino Acids 

Buffers 

Benzonase® endonuclease 


Tentacle Media 

Ion Exhangers 

Hydrophobic Resins 

Affinity Resins 

Size Exclusion 

Process and Production 

Chromatography 


LiChroprep® 

LiChrospher® 

Other Sorbents 

PharmPrep® 

Aluminium Oxide 

Preparative Columns 

Hibar® Columns 

Lobar® Columns 

Customized Columns 

Column packing Stands 


Contract Separation 

Biochemicals 

Enzymes 

11

Amino Acids for Processing 

Amino acids are the building blocks of life, the substances 

that proteins are made of. L-Amino acids and their derivatives 

are key compounds in the life sciences. They are used 

in large quantities as raw materials, excipients and active 

ingredients in the pharmaceutical and chemical industries. 

In the area of biotechnology, they are essential components 

in practically all fermentation and cell culture processes. 

The quality demanded of such amino acids for use in 

pharmaceutical applications is prescribed by procedures 

contained in the most common used pharmacopoeias (DAB, 

Ph Eur, USP, etc.). In addition, when used in biotechnological 

applications, particularly for the manufacture of pharmaceutical 

active ingredients, further requirements have to 

be fulfilled with respect to product properties. Our stringent 

analytical control procedures ensure high and uniform 

quality and excellent batch-to-batch consistency. 

Amino acids for pharmaceutical applications 

These are tested according to the relevant current 

pharmacopoeias Ph Eur, BP and USP as mentioned in the 

product name. 

Amino acids for biochemistry 

These are mainly used in research and development. The 

quality and specifications of these products is therefore 

performed according to this application. Essential contents 

of the specifications are purity, optical purity, the absence 

of biological inhibitors (heavy metals, salts, etc.) and 

theabsence of foreign amino acids. 

12 

Product Cat.-No. Packsize 

N-Acetyl-L-cysteine for biochemistry 

N-Acetyl-DL-tryptophan 

extra pure low in endotoxins, Ph Eur, BP 

1.12422.0025 

1.12422.0100 

1.12422.1000 

1.12422.9010 

1.12488.1000 

1.12488.5000 

1.12488.9025 

25 g 

100 g 

1 kg 

10 kg 

1 kg 

5 kg 

25 kg 

N-Acetyl-DL-tryptophan for biochemistry 1.12423.0025 25 g 

ß-Alanine for biochemistry 

1.01008.0250 

1.01008.1000 

250 g 

1 kg 

DL-Alanine for biochemistry 

1.00963.0100 

1.00963.1000 

100 g 

1 kg 

1.01700.0100 100 g 

L-Alanine Ph Eur, USP 

1.01700.1000 1 kg 

1.01700.9010 10 kg 

1.01007.0025 25 g 

L-Alanine for biochemistry 

1.01007.0100 

1.01007.1000 

100 g 

1 kg 

1.01007.9010 10 kg 

4-Aminohippuric acid for biochemistry 

1.00084.0025 

1.00084.0100 

25 g 

100 g 

5-Aminolevulinic acid hydrochloride 1.24802.0500 500 mg 

for biochemistry 

1.24802.2500 2.5 g 

1.01587.0100 100 g 

L-Arginine Ph Eur, USP 

1.01587.1000 1 kg 

1.01587.9010 10 kg 

1.01542.0100 100 g 

L-Arginine for biochemistry 

1.01542.1000 1 kg 

1.01542.9010 10 kg 

1.01544.0250 250 g 

L-Arginine monohydrochloride 

1.01544.1000 1 kg 

extra pure Ph Eur, BP, USP 

1.01544.5000 5 kg 

1.01544.9010 10 kg 

1.01543.0050 50 g 

L-Arginine monohydrochloride 

1.01543.0250 250 g 


1.01543.1000 1 kg 

1.01543.9010 10 kg 

1.01565.0100 100 g 

L-Asparagine monohydrate extra pure 1.01565.1000 1 kg 

DAB 

1.01565.5000 5 kg 

1.01565.9010 10 kg 

L-Asparagine monohydrate 


1.01566.0100 

1.01566.1000 

1.01566.9010 

100 g 

1 kg 

10 kg 

1.00129.0100 100 g 

L-Aspartic acid extra pure Ph Eur, BP 

1.00129.1000 

1.00129.5000 

1 kg 

5 kg 

1.00129.9010 10 kg 

1.00126.0100 100 g 

L-Aspartic acid for biochemistry 1.00126.1000 1 kg 

1.00126.9010 10 kg 

L-Aspartic acid Monosodium salt 1.00142.0100 100 g 


1.00142.1000 1 kg 

L-Citrulline for biochemistry 

1.12117.0050 

1.12117.0250 

50 g 

250 g 

1.02838.0025 25 g 

L-Cysteine for biochemistry 

1.02838.0100 

1.02838.1000 

100 g 

1 kg 

1.02838.9010 10 kg 

L-Cysteine hydrochloride monohydrate Ph 

Eur, USP 

1.02735.0100 

1.02735.1000 

1.02735.9010 

100 g 

1 kg 

10 kg 

1.02839.0025 25 g 

L-Cysteine hydrochloride monohydrate 1.02839.0100 100 g 


1.02839.1000 1 kg 

1.02839.9010 10 kg 

1.02737.0100 100 g 

L-Cystine Ph Eur 

1.02737.1000 1 kg 

1.02737.9010 10 kg 

1.02837.0025 25 g 

L-Cystine for biochemistry 

1.02837.0100 

1.02837.1000 

100 g 

1 kg 

1.02837.9010 10 kg


L-Glutamic acid Ph Eur 

L-Glutamic acid for biochemistry 

L-Glutamine DAB, USP 

L-Glutamine for biochemistry 

Glycine cryst. Ph Eur, BP, USP 

Glycine GR for analysis 

Hippuric acid LAB 

L-Histidine Ph Eur, USP 

L-Histidine for biochemistry 

L-Histidine monohydrochloride extra pure 

Ph Eur,BP 

L-Histidine monohydrochloride 


L-Hydroxyproline for biochemistry 

L-Isoleucine Ph Eur, USP 

L-Isoleucine for biochemistry 

L-Leucine Ph Eur, USP 

L-Leucine for biochemistry 

L-Lysine monohydrate for biochemistry 

L-Lysine monohydrochloride extra pure Ph 

Eur, BP, USP 

L-Lysine monohydrochloride for 

biochemistry 

L-Methionine for biochemistry 

1.01791.0100 

1.01791.1000 

1.01791.9010 

1.00291.0100 

1.00291.0250 

1.00291.1000 

1.00291.9010 

1.00286.0100 

1.00286.1000 

1.00286.9010 

1.00289.0025 

1.00289.0100 

1.00289.1000 

1.00289.9010 

5.00190.1000 

5.00190.5000 

5.00190.9025 

1.04201.0100 

1.04201.0250 

1.04201.1000 

1.04201.5000 

1.04201.9025 

1.00296.0500 

1.00296.2500 

1.00296.9025 

1.04352.0100 

1.04352.1000 

1.04352.9010 

1.04351.0025 

1.04351.0100 

1.04351.1000 

1.04351.9010 

1.04354.0500 

1.04354.5000 

1.04350.0025 

1.04350.0100 

1.04350.0500 

1.04350.9010 

1.04510.0010 

1.04510.0100 

1.05357.0100 

1.05357.1000 

1.05357.9010 

1.05362.0025 

1.05362.0100 

1.05362.0500 

1.05362.9010 

1.05350.0100 

1.05350.1000 

1.05350.9010 

1.05360.0025 

1.05360.0250 

1.05360.9010 

1.12233.0100 

1.12233.1000 

1.05701.1000 

1.05701.5000 

1.05701.9025 

1.05700.0100 

1.05700.1000 

1.05700.9010 

1.05707.0025 

1.05707.0100 

1.05707.1000 

1.05707.9010 

100 g 

1 kg 

10 kg 

100 g 

250 g 

1 kg 

10 kg 

100 g 

1 kg 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

1 kg 

5 kg 

25 kg 

100 g 

250 g 

1 kg 

5 kg 

25 kg 

500 g 

2.5 kg 

25 kg 

100 g 

1 kg 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

500 g 

5 kg 

25 g 

100 g 

500 g 

10 kg 

10 g 

100 g 

100 g 

1 kg 

10 kg 

25 g 

100 g 

500 g 

10 kg 

100 g 

1 kg 

10 kg 

25 g 

250 g 

10 kg 

100 g 

1 kg 

1 kg 

5 kg 

25 kg 

100 g 

1 kg 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

L-Norleucine for biochemistry 1.24560.0250 250 mg 

L-Ornithine monohydrochloride 


DL-Phenylalanine for biochemistry 

L-Phenylalanine Ph Eur, USP 

1.06906.0025 

1.06906.0100 

1.06906.1000 

1.06906.9010 

1.07257.0025 

1.07257.0100 

1.07267.0100 

1.07267.1000 

1.07267.9010 

25 g 

100 g 

1 kg 

10 kg 

25 g 

100 g 

100 g 

1 kg 

10 kg 


L-Phenylalanine for biochemistry 

L-Proline Ph Eur, USP 

L-Proline for biochemistry 

L-Serine Ph Eur, USP 

L-Serine for biochemistry 

L-Threonine for biochemistry 

DL-Tryptophane for biochemistry 

L-Tryptophane extra pure 

Ph Eur, BP, USP 

L-Tryptophane for biochemistry 

L-Tyrosine Ph Eur, USP 

L-Tyrosine for biochemistry 

L-Valine for biochemistry 

For more information, products 

and qualities please ask for the 

Merck catalogue Chemicals 

Reagents or at Merck ChemDAT. 

We also welcome you on our 

homepage www.merck-lsp.de 

1.07256.0025 

1.07256.0100 

1.07256.1000 

1.07256.9010 

1.07430.0100 

1.07430.1000 

1.07430.9010 

1.07434.0010 

1.07434.0100 

1.07434.0500 

1.07434.9010 

1.07647.0100 

1.07647.1000 

1.07647.9010 

1.07769.0010 

1.07769.0100 

1.07769.1000 

1.07769.9010 

1.08411.0010 

1.08411.0100 

1.08411.1000 

1.08411.9010 

1.08375.0025 

1.08375.0100 

1.08396.0100 

1.08396.1000 

1.08396.5000 

1.08374.0010 

1.08374.0100 

1.08374.0500 

1.08374.9010 

1.08378.0100 

1.08378.1000 

1.08378.9010 

1.08371.0025 

1.08371.0100 

1.08371.1000 

1.08371.9010 

1.08495.0025 

1.08495.0100 

1.08495.1000 

1.08495.9010 

25 g 

100 g 

1 kg 

10 kg 

100 g 

1 kg 

10 kg 

10 g 

100 g 

500 g 

10 kg 

100 g 

1 kg 

10 kg 

10 g 

100 g 

1 kg 

10 kg 

10 g 

100 g 

1 kg 

10 kg 

25 g 

100 g 

100 g 

1 kg 

5 kg 

10 g 

100 g 

500 g 

10 kg 

100 g 

1 kg 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

13

14 

Biological Buffers for Processing 

Working with biologically active 

molecules invariably involves the use 

of buffer solutions. The task of the 

buffer is to create a defined environment 

for the biomolecules so that they 

can be kept in a stable and native 

form and stays fully active. The buffer 

itself, however, should not exert any 

influence on the compound. Equally 

important, the buffer should not 

contain any impurities that may have 

a negative effect on the activity or 

stability of the molecule. Hence, only 

highly-purified substances of defined 

specifications should be used to ensure 

reproducible processes on a lab or 

production scale. 

Merck supplies buffers in various 

grades. The differences between them 

rarely involve purity itself but more 

the additional parameters that are 

checked prior to delivery. For 

example, whilst the nucleases may be 

an important quality criterion for 

biotechnological applications, the 

fulfilling of the requirements of a 

particular pharmacopoeia may well be 

critical when it comes to manufacturing 

a pharmaceutical active ingredient. 

However, for certain applications, 

it may be desirable to specify 

additional quality parameters not 

included in our “Certificate of 

Analysis”. Should you require any 

special testing, we will be able to offer 

you a customized product in most 

cases. The same applies to individual 

package sizes. We will be glad to 

prepare such a special service for your 

convenience. Please contact us.

Buffer substances pH-Range Cat.-No. Pack-size 

ACES 

N-(2-Acetamido)-2-aminoethanesulfonic acid 

ADA 

N-(2-Acetamido)iminodiacetic acid 

BES 

N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid 

BICINE 

N,N-Bis(2-hydroxyethyl)-glycine 

BIS-TRIS 

2,2-Bis(hydroxyethyl)-iminotris(hydroxymethyl)-methane 

CHES 

2-(Cyclohexylamino)ethanesulfonic acid 

6.1 – 7.6 

5.9 – 7.3 

6.4 – 7.8 

7.6 – 9.0 

5.8 – 7.3 

8.0 – 10.0 

Glycylglycine 7.5 – 8.9 

HEPES 

2-[4-(2-Hydroxyethyl-1-piperazine)]ethanesulfonic acid 6.5 – 8.5 

HEPES for biotechnology 

2-[4-(2-Hydroxyethyl-1-piperazine)]ethanesulfonic acid 

HEPES Sodium 


monosodium salt 

HEPPS 

3-[4-(2-Hydroxyethyl-1-piperazinyl)]-propanesulfonic acid 

6.5 - 8.5 

6.5 – 8.5 

7.3 – 8.7 

Imidazol 6.2 – 7.8 

MES 

2-Morpholinoethanesulfonic acid monohydrate 

MOPS 

3-Morpholinopropanesulfonic acid 

PIPES 

Piperazine-1,4-bis(2-ethanesulfonic acid) 

PIPES Sodium 

Piperazine-1,4-bis(2-ethanesulfonic acid) monosodium salt 

TES 

N-[Tris(hydroxymethyl)-methyl]-2-aminoethanesulfonic 

TRICINE 

N-[Tris(hydroxymethyl)-methyl]-glycine 

TRIS Ph Eur, BP, USP 

Tris(hydroxymethyl)aminomethane 

TRIS GR for analysis, ACS 


TRIS LAB 


TRIS-hydrochloride GR for analysis 

Tris(hydroxymethyl)aminomethane hydrochloride 

5.6 – 6.7 

6.5 – 7.9 

6.1 – 7.5 

6.1 – 7.5 

6.8 – 8.2 

7.2 – 9.2 

7.1 – 9.0 

7.1 – 9.0 

7.1 – 9.0 

7.1 – 9.0 

1.15226.0025 

1.15226.0250 

1.15226.9010 

1.15227.0025 

1.15227.9010 

1.15228.0025 

1.15228.0250 

1.15228.9010 

1.01910.0025 

1.01910.0250 

1.01910.9010 

1.03252.0025 

1.03252.0250 

1.15229.0025 

1.15229.0250 

1.04233.0010 

1.04233.0100 

1.04233.1000 

1.04233.9010 

1.10110.0025 

1.10110.0250 

1.10110.1000 

1.10110.9010 

1.10110.9025 

1.17839.0100 

1.17839.1000 

1.17839.9010 

1.15231.0025 

1.15231.0250 

1.15231.2500 

1.15231.9010 

1.15230.0025 

1.15230.0250 

1.15230.2500 

1.15230.9010 

1.04716.0050 

1.04716.0250 

1.04716.1000 

1.04716.9025 

1.06126.0025 

1.06126.0250 

1.06126.9010 

1.06129.0025 

1.06129.0250 

1.06129.9010 

1.10220.0025 

1.10220.0250 

1.10220.1000 

1.10220.9010 

1.15232.0025 

1.15232.0250 

1.10695.0025 

1.10695.0250 

1.10695.9010 

1.08602.0025 

1.08602.0250 

1.08602.9010 

1.08386.1000 

1.08386.5000 

1.08386.9025 

1.08382.0100 

1.08382.0500 

1.08382.1000 

1.08382.2500 

1.08382.5000 

1.08382.9025 

1.08387.0500 

1.08387.2500 

1.08219.0100 

1.08219.1000 

1.08219.9010 

1.08219.9025 

25 g 

250 g 

10 kg 

25 g 

10 kg 

25 g 

250 g 

10 kg 

25 g 

250 g 

10 kg 

25 g 

250 g 

25 g 

250 g 

10 g 

100 g 

1 kg 

10 kg 

25 g 

250 g 

1 kg 

10 kg 

25 kg 

100 g 

1 kg 

10 kg 

25 g 

250 g 

2.5 kg 

10 kg 

25 g 

250 g 

2.5 kg 

10 kg 

50 g 

250 g 

1 kg 

25 kg 

25 g 

250 g 

10 kg 

25 g 

250 g 

10 kg 

25 g 

250 g 

1 kg 

10 kg 

25 g 

250 g 

25 g 

250 g 

10 kg 

25 g 

250 g 

10 kg 

1 kg 

5 kg 

25 kg 

100 g 

500 g 

1 kg 

2.5 kg 

5 kg 

25 kg 

500 g 

2.5 kg 

100 g 

1 kg 

10 kg 

25 kg 

For more information, 

products and qualities please 

ask for the Merck catalogue 

Chemicals Reagents or at 

Merck ChemDAT®. We also 

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Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

15

16 

Benzonase ® endonuclease – 

the Smart Solution for DNA Removal 

Benzonase® endonuclease is a 

genetically engineered endonuclease. 

With its ability to rapidly hydrolyze 

all types of nucleic acids and its extremely 

high purity, it is the ideal 

choice for a wide range of applications 

in biochemical research and biotechnology. 

Benzonase® endonuclease brings 

benefits to the industrial-scale 

manufacturing of recombinant 

proteins and other biologicals, 

irrespective of the type of production 

organism or cell-line. 

Benzonase® endonuclease... 

... attacks and degrades all forms of 

DNA and RNA. 

... is free of detectable proteolytic 

activity. 

... is effective over a wide range of 

operating conditions. 

... possesses an exceptionally high 

specific activity. 

... is manufactured under strictly 

regulated conditions in order to meet 

industrial requirements for a reliable 

supply and constant high quality. 

... is ideal for a variety of applications 

including: 

- purification of proteins and other 

biologicals. 

- reduction of viscosity caused by 

nucleic acids. 

- sample preparations in electrophoresis 

and chromatography. 

FDA guidelines for the manufacture of 

recombinant biologicals for therapeutic 

use demand that nucleic acid contamination 

should be limited to 10 pg 

per dose in the end product. 

Benzonase® endonuclease, when used 

under appropriate reaction conditions, 

will degrade all nucleic acid sequences 

down to oligonucleotides of approximately 

3 to 5 base pairs in length – 

which is significantly below the hybridisation 

limit – enabling recombinant 

proteins to meet the FDA 

guidelines for nucleic acid contamination. 

The ability of Benzonase® endonuclease 

to rapidly hydrolyze nucleic acids 

makes the enzyme an ideal choice for 

reducing cell lysate viscosity – both in 

the research laboratory and the manufacturing 

plant. 

Using Benzonase® endonuclease to 

reduce viscosity results in a number 

of benefits including: 

· Reduced processing time 

· Increased yield of protein products 

· Improved separation of pellet and 

supernatant in centrifugations 

· Easier filtration of solutions, 

especially ultrafiltration 

· Increased efficiency of chromatographic 

purification steps 

(e.g. in expanded bed adsorption). 

Benzonase® endonuclease may be 

used with all methods of cell lysis, including 

lysozyme treatment freezethawing 

procedures and high-pressure 

homogenization.

Cat.-No. Designation Pack-size 

1.01653.0001 Benzonase® endonuclease purity grade II (≥ 90 %) 10,000 U/vial 

1.01653.0002* ≥ 25 U/µl, for biochemistry 


1.01654.0002* ≥ 250 U/µl, for biotechnology 



1.01694.0001 Benzonase® endonuclease purity grade I (≥ 99 %) 10,000 U/vial 

1.01694.0002* ≥ 25 U/µl, for biochemistry 





1.01697.0010 Benzonase® endonuclease purity grade I (≥ 99 %) 5,000,000 U/vial** 


1.01681.0001 Benzonase® endonuclease ELISA kit II for the 5 plates (8 x 12) 

1.01681.0002* immunological detection of Benzonase® endonuclease plus reagents 

In addition to the above, special package sizes are available on request. 

Benzonase® is covered by several patents, 

i.e. US Patent No.5, 173, 418, EP Patent No. 0229866 B1 

Notes: 

* This Cat.-No. is valid for USA and Canada only. 

** This package size is only available on request. 

Detailed information 

you will find in our 

product brochure 

“Benzonase® endonuclease”. 

Please order your free 

copy right now: 


Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

17

18 


Downstream processing of proteins 

and other biomolecules represents a 

key role in the production of therapeutic 

substances. The major requirement 

is to maintain the biological activity of 

these biomolecules during purification, 

i.e. maintaining the intact native 

structure through-out the separation 

process. Therefore, to avoid protein 

denaturation, most biochromatography 

unit operations employ aqueous 

buffer systems. After the complete 

purification process has been developed 

on a small scale in the lab, the 

protocols, transferred to large-scale 

production, must give the same results. 

Normally, the large-scale production 

of bio-molecules is a multi-step process 

where column chromatography 

plays a crucial role. The overall separation 

and purification sequence for a 

protein typically involves 5 -10 unit 

operations. Each step must be operated 

with the highest possible efficiency in 

order to optimize product yield, ensure 

feed stream 

centrifugation, filtration 

capture 

high throughput; reduction of volume 

intermediate purification 

higher resolution; removal of host cell proteins 

final polishing 

high resolution; removal of aggregates and DNA 

process reproducibility and minimize 

process economics. The purification 

strategy is to determine experimentally 

the shortest route from the starting 

material to the final product that meets 

the required specifications for contaminants 

and impurities. A schematic 

overview is given in Figure 1. 

Fractogel® EMD media enable the user 

to meet all relevant requirements. 

Capture steps require high flow rates 

with no loss of capacity. All Fractogel® 

EMD media provide excellent results 

for ion exchange, hydrophobic interaction, 

affinity and size exclusion 

chromatography. High capacities, high 

recoveries and high chemical and 

mechanical stability of Fractogel® EMD 

provide the downstream specialist a 

powerful tool for protein separations. 

At least five times the number of cycles 

can be run on Fractogel® EMD resins 

using the same column packing as 

with soft gels based on carbohydrate 

matrices. 

Figure 1: 

Flow chart of 

downstream 

processing

New Robust Tentacle Media 

Tentacle surface technology, based on the grafting 

of functionally substituted acrylamides to 

the surface of porous particles, has now been 

transferred to a novel hydrophilic vinyl polymer 

resin. The resulting Fractoprep® ion exchange 

media show excellent protein binding capacities 

at high flow rates, with much improved mechanical 

and chemical stability. The resins are extremely 

resistant to sodium hydroxide treatment, 

the most important clean-in-place step in almost 

every production process and, in addition, pro- 

Ion Exchange Chromatography on Fractogel® EMD Tentacle Resins 

In ion exchange chromatography, charged molecules 

are separated on column materials that 

carry an opposite charge. The ionic groups of ion 

exchange resins are covalently bound to the gel 

matrix and are partially neutralized by counter 

ions present in the buffer. Because proteins are 

multivalent anions or cations, at low salt concentrations 

they will bind to stationary phases 

that carry the opposite charge. The binding is 

strongly influenced by the pH of the buffer. For 

efficient use of ion exchange chromatography, it 

is important to operate at pH values where the 

exchangers are mostly ionised and the biopolymers 

have a net charge (Table 1). Excessively 

high salt concentrations, however, cause 

shielding of the charges on the protein surface 

and effective binding of protein to an exchanger 

can no longer take place. Once bound to the 

matrix, biomolecules are in turn displaced by an 

increasing salt gradient or pH changes. Proteins 

with different net charges can be separated. 

Desorption of proteins from the column begins 

only with increasing salt concentrations or pH 

changes, when the protein no longer binds. The 

compounds that have a higher charge density are 

bound more firmly to the column while the 

others elute more rapidly. In contrast to strong 

ion exchangers, which can be used over a wide 

pH range, weak ion exchangers have changing 

degrees of ionization depending on the pH. Thus, 

the pH range over which you can use the ion 

exchangers is limited by these properties., e.g. 

Fractogel® EMD COO - can be used above pH 5, 

Fractogel® EMD DEAE below pH 8.5. The weak 

vide other features essential for application in 

bio-pharmaceutical production. Due to their 

excellent mechanical rigidity, Fractoprep® media 

are easy to handle and can be packed in large 

production columns without any problems. For a 

complete list of references citing tentacle media 

products, please visit our web-page. This list of 

references includes papers using all the different 

types of Fractogel® EMD and Fractoprep® media. 

ion exchangers are used, in general, in situations 

where the pH of the load is consistent and doesn't 

change from run to run. Strong ion exchangers 

that are charged regardless of pH are generally 

used in production were the feed streams 

sometimes change. 

Recently, a new generation of Fractoprep® 

tentacle media was launched. Fractoprep® beads 

have a particle size range between 30 and 150 µm 

and were designed for capture steps. The new 

Fractoprep® ion exchangers provide high productivity 

especially in the capture step. Large 

pores enable a free diffusion of proteins into the 

beads and allow high throughput at very low 

back pressure. Three different types of ion 

exchangers, Fractoprep® DEAE, Fractoprep® 

TMAE and Fractoprep® SO 3 - , extend the product 

range of the well-known Fractogel® tentacle 

resins. 

Product Operating range Capacity 

Fractogel® EMD TMAE BSA 100 mg/ml 

Fractogel® EMD TMAE Hicap pH 6-10 BSA 180 mg/ml 

Fractoprep® TMAE BSA 130 mg/ml 

Fractogel® EMD DEAE BSA 100 mg/ml 

pH 6-8.5 

Fractoprep® DEAE 

BSA 130 mg/ml 

Fractogel® EMD DMAE pH 6-8.5 BSA 100 mg/ml 

– 

Fractogel® EMD SO3 

Lysozyme 130 mg/ml 

Fractogel® EMD SE Hicap pH 4-8 Lysozyme 160 mg/ml 

– 

Fractoprep® SO3 

Lysozyme 100 mg/ml 

Fractogel® EMD COO – 

Table 1 

pH 5-8 Lysozyme 100 mg/ml 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

19

20 

matrix 

protein 

pore structure 

Advantages of the Tentacle Ion Exchangers 

With most ion exchange gels the functional 

groups are fixed directly, or with 

short linkers, onto the surface of the 

matrix. On any given gel matrix, only 

a certain number of ionic groups can 

be attached because of the chemistries 

used and the limited inner surface of 

the matrix. Once attached, the functional 

groups must be accessible in 

order for biopolymers to bind. The 

spatial requirements for this binding 

dictates the limit of the protein binding 

capacity for these kinds of ion exchangers. 

With tentacle ion exchangers, 

however, the functional groups are 

covalently anchored to the matrix via 

flexible linear polymer chains. Therefore, 

the binding capacity for proteins 

functional groups 

Figure 2: 

left – conventional 

ion exchanger 

right – tentacle ion 

exchanger 

can be increased significantly. The 

tentacles allow biopolymers greater 

accessibility to the functional groups 

due to less steric hindrance (Figure 2). 

Especially for large proteins, tentacle 

media provide higher binding capacities 

compared to conventional 

supports. Due to the good selectivity 

and high efficiency, columns packed 

with tentacle media provide good 

chromatographic results and highly 

concentrated fractions can be obtained. 

The mechanical stability of all tentacle 

media also allows the use of high flow 

rates without a significant loss of 

capacity. 

Fractogel® EMD media as well as 

Fractoprep® media are pressure-stable 

and can be easily regenerated with 

sodium hydroxide. 

The main characteristics are: 

· High dynamic protein binding 

capacity 

· Chemical stability (acids, alkali) 

· Mechanical stability (pressure stable) 

· Large pore diameter ( > 800 Å ) 

·Resistance to micro-organisms 

· Reduced non-specific interactions 

· High selectivity 

· Availability at constant quality

Fractogel®EMD tentacle media / Ordering information 

Designation Cat.-No. Particle size Content 

Chromatography type: strong anion exchanger 

Fractogel ® EMD TMAE (M) 

Fractogel ® EMD TMAE Hicap (M) 

Fractogel ® EMD TMAE (S) 

Fractoprep® TMAE 

Chromatography type: weak anion exchanger 

Fractogel® EMD DEAE (M) 

Fractogel® EMD DEAE (S) 

Fractogel® EMD DMAE (M) 

Fractogel® EMD DMAE (S) 


Chromatography type: strong cation exchanger 

Fractogel® EMD SO 3 – (M) 

Fractogel® EMD SE Hicap (M) 

Fractogel® EMD SO 3 – (S) 

Fractoprep® SO 3 

Chromatography type: weak cation exchanger 

Fractogel® EMD COO – (M) 

Fractogel® EMD COO – (S) 

1.16881.0100 100 ml 

1.16881.0500 40 – 90 µm 500 ml 

1.16881.5000 5000 ml 

1.10316.0100 100 ml 

1.10316.0500 40 – 90 µm 500 ml 

1.10316.5000 5000 ml 

1.16887.0100 

1.16887.0500 

20 – 40 µm 

100 ml 

500 ml 

1.17973.0100 100 ml 

1.17973.1000 30 – 150 µm 1000 ml 

1.17973.5000 5000 ml 

1.16883.0100 100 ml 

1.16883.0500 40 – 90 µm 500 ml 

1.16883.5000 5000 ml 

1.16888.0100 

1.16888.0500 

20 – 40 µm 

100 ml 

500 ml 

1.16884.0100 100 ml 

1.16884.0500 40 – 90 µm 500 ml 

1.16884.5000 5000 ml 

1.16889.0100 

1.16889.0500 

20 – 40 µm 

100 ml 

500 ml 

1.17971.0100 100 ml 

1.17971.1000 30 – 150 µm 1000 ml 

1.17971.5000 5000 ml 

1.16882.0100 100 ml 

1.16882.0500 40 – 90 µm 500 ml 

1.16882.5000 5000 ml 

1.14894.0100 100 ml 

1.14894.0500 40 – 90 µm 500 ml 

1.14894.5000 5000 ml 

1.16890.0100 

1.16890.0500 

20 – 40 µm 

100 ml 

500 ml 

1.17972.0100 100 ml 

1.17972.1000 30 – 150 µm 1000 ml 

1.17972.5000 5000 ml 

1.16886.0100 100 ml 

1.16886.0500 40 – 90 µm 500 ml 

1.16886.5000 5000 ml 

1.16891.0100 

1.16891.0500 

20 - 40 µm 

100 ml 

500 ml 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

21

22 

Fractogel® EMD Tentacle Phases for Hydrophobic Interaction Chromatography 

High performance hydrophobic interaction chromatography 

is a powerful technique for the isolation 

of proteins on an analytical and preparative 

scale. The method of hydrophobic interaction 

chromatography (HIC) is based on the interaction 

between hydrophobic ligands on the chromatographic 

support and hydrophobic areas located on 

the surface of proteins. The hydrophobic feature 

of a given protein is caused by certain amino acid 

residues like isoleucine, valine, leucine, phenylalanine 

and others. 

Although most of the hydrophobic amino acid 

residues are located in the center of the molecule, 

recent structure analyses have shown 

hydrophobic patches on the protein’s surface. 

These areas are often correlated with the biological 

function of the protein. The hydrophobic 

character of a protein is promoted by high salt 

and increased temperatures. 

Advantages of HIC tentacle materials 

The high selectivity of Fractogel® EMD Propyl (S) 

Metal Chelate Affinity Chromatography on Fractogel® EMD Chelate 

Advantages of tentacle media for metal chelate 

affinity chromatography 

The high efficiency of the method is based on the 

interaction of a covalently bound chelating ligand 

immobilized on a chromatographic support with 

proteins containing a large number of histidine 

residues. Some recombinant proteins have His 

tags engineered at the end of the protein sequence 

to facilitate purification on IMAC resins. Iminodiacetic 

acid has been chosen as the metal-chelating 

ligand for Fractogel® EMD Chelate. The 

iminodiacetic acid residue is suitable as a chelating 

compound because a bidentate chelating 

moiety remains free after immobilization, onto 

which a metal ion can be co-ordinated. Various 

metal ions can be immobilized on the stationary 

phase (e.g. Cu 2+ ; Ni 2+ ; Co 2+ ; Zn 2+ ). Free coordination 

sites of the metal ions are used to bind 

different proteins and peptides. 

Compared to conventional affinity gels synthesised 

with spacer molecules, Fractogel® EMD 

and Fractogel® EMD Phenyl (S) allow efficient 

purification steps. Both phases, with a particle 

size of 20-40 µm, are mechanically stable and 

resistant to biological and chemical degradation. 

The functional groups are on linear polymer 

chains. The ligands differ with respect to their 

hydrophobicity. Ligand density of the functional 

groups bound to the matrix is optimised by the 

tentacle technology so that excessive hydrophobicity 

is avoided. Strong hydrophobic resins 

inhibit recovery and resolution. 

Application areas 

Both hydrophobic phases are suitable for a wide 

range of applications. The main application areas 

for HIC is the purification of cytosolic proteins, 

antibodies, and recombinant proteins. Membrane 

proteins can also be isolated on Fractogel® EMD 

Propyl (due to its weak hydrophobicity this resin 

is more suitable). For the elution of strong 

hydrophobic proteins, a detergent should be 

used. In addition HIC is suitable for the removal 

or exchange of non-ionic detergents. 

Chelate allows the binding of larger amounts of 

proteins. Because the immobilized metal ion can 

be arranged in the best position relative to the 

binding sites on the protein’s surface, proteins 

can be bound more tightly to Fractogel® EMD 

Chelate than comparable conventional gels. 


The existence of surface-accessible histidine 

residues is important for the binding of a native 

protein. As recombinant proteins become more 

important, expression systems using a polyhistidine 

tag for the protein enable rapid isolation 

of those proteins by metal chelating affinity 

chromatography. With peptides, the alpha amino 

group also plays a role, so that peptides can be 

retained even if no histidine residues are present. 

In the case of peptides, other metal binding amino 

acids like cysteine and tryptophan contribute to 

the binding.

Thiophilic Adsorption Chromatography on Fractogel® EMD TA 

Advantages of tentacle-mediated 

thiophilic adsorption 

The binding mechanism of thiophilic adsorption 

chromatography is based on the specific interaction 

of a covalently bound sulphur-containing 

ligand immobilized on a chromatographic support 

with proteins or peptides. An electron 

donor-acceptor mechanism between the affinity 

ligand and tryptophan residues on the surface of 

the target molecule is responsible for the selective 

binding. Fractogel® EMD TA is designed specifically 

for the efficient isolation of antibodies. The 

synthesis is performed according to the tentacle 

technology where the group-specific ligands are 

present in high density. Thus, the thiophilic tentacle 

material has a high protein-binding capacity 

and is suitable for antibody purification on 

an analytical as well as a preparative scale. Due to 

the steric accessibility of the ligand, the resin provides 

minimized non-specific interactions with 

proteins combined with high protein binding capacities. 

The chemistry of the 3-S type ligand that 

is used for Fractogel® EMD TA allows significantly 

better thiophilic binding compared to the 2-S 

types which have more hydrophobic interaction. 


Thiophilic adsorption chromatography is specifically 

designed for the purification of immunoglobulins 

(monoclonal and polyclonal antibodies) 

including single-chain antibodies and chicken 

antibodies. Albumins are not adsorbed on thiophilic 

media, which often simplifies the effective 

separation of antibodies. The binding of the protein 

takes place mainly via accessible tryptophan 

and/or phenylalanine residues. Corresponding 

motifs can be observed within conserved regions 

of various antibodies. Antibodies of the IgM subclass 

can also bind to a Fractogel® EMD TA 

column. The binding of antibodies from different 

species to thiophilic adsorption supports offers 

advantages over protein A. Elution conditions at 

physiological pH values enable high recoveries of 

biologically active antibodies. 

Fractogel® EMD tentacle gels for HIC / Ordering information 


Chromatography type: weak HIC 

Fractogel ® EMD Propyl (S) 

Chromatography type: strong HIC 

Fractogel ® EMD Phenyl (S) 

1.10085.0100 

100 ml 

1.10085.0500 

20 – 40 µm 

500 ml 

1.16197.0100 

1.16197.0500 

20 – 40 µm 

100 ml 

500 ml 

Bulk quantities on request. 

Fractogel® EMD tentacle affinity gels / Ordering information 


Chromatography type: metal affinity chromatography 

Fractogel ® 1.10338.0250 250 ml 

EMD Chelate (M) 

1.10338.0500 40 – 90 µm 500 ml 

1.10338.5000 5000 ml 

Chromatography type: thiophilic adsorption 

Fractogel ® EMD TA (S) 

Chromatography type: affinity chromatography 

Fractogel® EMD Amino (M) 

1.16473.0025 25 ml 

1.16473.0250 20 – 40 µm 250 ml 

1.16473.1000 1000 ml 

1.14893.0500 

1.14893.5000 

40 – 90 µm 

500 ml 

5000 ml 

Bulk quantities on request. 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

23

24 

Molecular Size Exclusion Chromatography on Fractogel® EMD BioSEC (S) 

This method, also known as gel filtration 

(GPC) or molecular sieve chromatography 

is based on the different size 

and shape of proteins. Proteins of 

different sizes penetrate into the internal 

pores of the beads to different 

degrees. Small protein molecules are 

retarded by the column while large 

molecules pass through more rapidly. 

A column packed with Fractogel® EMD 

BioSEC can be used for the separation 

of proteins in the range between 5 kDa 

and 1,000 kDa (Figure 3). The mechanical 

stability of the resin allows higher 

flow rates compared to conventional 

soft gels. This is very helpful during 

regeneration and equilibration of the 

column. The pressure stability also enables 

the packing of larger columns 

that are needed for polishing steps in 

the production of bio-molecules. 

Figure 3: Separation of a complex mixture on 

Fractogel® EMD BioSEC (S) with a column 

dimension of 1000 x 50 mm. The sample 

contained ferritin (1), immunoglobulin G (2), 

transferrin (3), ovalbumin (4), myoglobin (5), 

aprotinin (6) and vitamin B12 (7). 5 ml of the 

mixture were injected onto the column at a 

flow rate of 3 ml/min (eluent: 20 mM sodium 

phosphate buffer, 0.1 M NaCl pH 7.2). 


SEC is very easy to perform up through 

production scale and the results are 

very reproducible. SEC is generally 

performed late in the process when the 

number of protein contaminants are 

small, as in a “polishing” step. The 

goal is the final removal of possible 

impurities such as structurally similar 

or closely related forms of the product 

(aggregates, deamidated or oxidised 

product, etc.), host proteins, reagents 

and buffer substances, leachables from 

chromatographic supports, endotoxins, 

nucleic acids (DNA, RNA) or 

viruses. However, if a target protein is 

extremely large or small, SEC can be 

utilized earlier in the purification process. 

Principal applications of SEC on Fractogel® 

EMD BioSEC are purification of 

cytosolic proteins as well as recombinant 

proteins and, simultaneously, the 

determination of their molecular mass 

after the column has been calibrated. 

Membrane proteins can also be isolated 

by this technique and SEC has been 

used in blood plasma fractionation. 

With the introduction of recombinant 

proteins there has been an emphasis 

on the determination of aggregates 

and other by-products that co-purify 

with the target protein. Size exclusion 

chromatography is also very useful for 

the purification of monoclonal and 

polyclonal antibodies. Albumins with 

a molecular weight of about 60 kDa 

elute earlier than IgG or IgM molecules. 

Since no gradient elution has to be 

applied, a programmable gradient 

mixing system is not necessary and 

comparatively simple equipment is 

needed for operation. In addition, the 

method can be easily integrated into 

purification schemes and most of the 

operational steps can be automated.

Fractogel® EMD BioSEC (S) / Ordering information 


Chromatography type: size exclusion chromatography 

Fractogel® EMD BioSEC (S) 

Regulatory Support Files 

Commitment to the quality of Fractogel® EMD 

and Fractoprep® process media for the benefit 

and success of our customers is a guiding 

element of our corporate strategy. One important 

product-related service is to provide detailed 

product information. All data are collected in a 

regulatory support file (RSF) which our customers 

find helpful in validating their production 

processes. The RSF contains all testing procedures, 

the results of stability studies as well as 

documentation concerning the lifetime of the 

resins. The production of the tentacle media 

results in reliable products with reproducible 

specifications within a narrow range as given 

in the Certificate of Analysis. A Certificate of 

Analysis is delivered with each batch on request. 

In addition, tentacle process media are covered 

by master file numbers (BB-MF No.) to facilitate 

regulatory filing requirements of end-users. 

FDA Registration numbers of Fractogel® media 

1.10317.0250 250 ml 

1.10317.1000 20 – 40 µm 1000 ml 

1.10317.5000 5000 ml 

Product Cat.-No. BB-MF No. 

Fractogel ® EMD TMAE S, M, Hicap 16881, 16887, 10316 4140 

Fractogel ® EMD SO 3 - , S, M 16882, 16890 4384 

Fractogel ® EMD DEAE, S, M 16883, 16888 4752 

Fractogel ® EMD COO - , S, M 16886, 16891 7193 

Fractogel ® EMD BioSEC S 10317 8114 

Fractogel ® EMD DMAE S, M 16889, 16884 8965 

Fractogel ® EMD Chelate S, M 16426, 10338 8254 

Fractoprep® TMAE 17973 In prep. 

Fractoprep® DEAE 17971 In prep. 

Fractoprep® SO 3 17972 In prep. 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

25

26 

Process and Production Chromatography 


The basis for reproducible chromatography 

in laboratory and production 

Preparative column chromatography 

is a separation and purification method 

that plays an important role in purifying 

valuable compounds in research, 

pilot plant operation and production. 

This method can be used not only to 

purify substances to a very high degree, 

but to do this in a rapid and 

economical way. 

In practice, standardized sorbents are 

required. These provide a high degree 

of method reliability, direct transfer 

from analytical scale and optimised 

throughput per unit of time. Only silica 

gels with a defined pore structure fulfill 

the requirements of process chromatography. 

Merck has specialized over decades in 

the manufacture of standardised silica 

gel packing materials for preparative 

chromatography and offers a wide 

range of customized sorbents. The product 

range is completed with several 

special types, e.g. silica gels of the 

type LiChroprep® or highly purified 

acid-washed silica gels. 

Such gels provide the user with unique 

method reliability. Regardless of which 

chromatography process is used 

(HPTLC, HPLC, preparative chromatography), 

direct and rapid transfer to a 

production scale is guaranteed and 

without risk. This is ensured by the 

fact that identical raw materials are 

used for all stationary phases, thus 

providing identical chromatographic 

selectivity. In order to be able to 

achieve this goal, Merck produces all 

silica gel 60 packing materials for 

analysis, pilot plant processes and 

production using the same silica gel 

raw material of specified surface area 

and a defined specific pore volume. 

Once a particular silica gel raw material 

has been tested with respect to its suitability 

for pore structure, specific pore 

volume, specific surface, chemical 

purity and other requirements for chromatography, 

it is classified into various 

categories and given defined particle 

size ranges. 

These particle size ranges are selected 

in such a way that an optimum in separating 

performance and permeability 

results for the chromatographic 

process used (DC, HPTLC, HPLC, preparative 

chromatography). 

Thus, once a successful configuration 

has been found on an analytical scale, 

it can be transferred rapidly to processes 

on a pilot plant or production 

scale. Merck guarantees direct scaleup 

without compromise. 

The selectivity of Merck’s silica gels 

for analytical and preparative applications 

is identical. Resolution, however, 

decreases, according to theory, 

with increasing particle size. Improved 

separation can be achieved at the 

expense of longer retention times and 

if the selectivity of the entire system is 

optimized using another mobile phase. 

Practice has shown that for chromatographic 

separations in production, 

silica gel 60 types i.e. silica gels of a 

mean pore size of 60 Å and in particle 

size ranges 40–63 µm and 63–200 µm 

are excellent for the separation of 

complex mixtures.

Merck quality: 

Customized and without 

compromise, for a high 

reliability in production 

Silica gels - standard / Ordering information 

Designation Cat.-No. Particle size pH Package Contents 

Silica gel 40 

1.10180.1000 

1 kg 

63 – 200 µm (70-230 mesh ASTM) 5.5 ± 0.5 Plastic 

1.10180.5000 5 kg 

Silica gel 40 

1.10181.1000 

1.10181.9025 

200 – 500 µm (35 – 70 mesh ASTM) 5.5 ± 0.5 Plastic 

1 kg 

25 kg 

1.15111.1000 1 kg 

Silica gel 60 1.15111.2500 15 – 40 µm 7.0 ± 0.5 Plastic 2.5 kg 

1.15111.9025 25 kg 

1.09385.1000 1 kg 

Silica gel 60 

1.09385.2500 

1.09385.5000 


2.5 kg 

5 kg 

1.09385.9025 25 kg 

1.07729.1000 1 kg 

Silica gel 60 1.07729.5000 < 63 µm (>230 mesh ASTM) 7.0 ± 0.5 Plastic 5 kg 

1.07729.9025 25 kg 

Silica gel 60 

1.15101.1000 

1.15101.9025 


1 kg 

25 kg 

1.07734.1000 1 kg 

Silica gel 60 

1.07734.2500 

1.07734.5000 


2.5 kg 

5 kg 

1.07734.9025 25 kg 

Silica gel 60 

extra pure 

1.07754.0500 

1.07754.1000 


500 g 

1 kg 

1.07733.0500 500 g 

Silica gel 60 

1.07733.1000 

1.07733.5000 


1 kg 

5 kg 

1.07733.9025 25 kg 

Silica gel 100 


1.10184.0500 63 – 200 µm (70 – 230 mesh ASTM) 6.5 ± 0.5 Plastic 500 g 

1.10184.5000 5 kg 

1.10185.0500 200 – 500 µm (35 – 70 mesh ASTM) 6.5 ± 0.5 Plastic 500 g 

1.10185.9025 25 kg 

Silica gel 60 F254 1.10757.1000 63 – 200 µm (70 – 230 mesh ASTM) 7.0 ± 0.5 Plastic 1 kg 

Packaging 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

27

28 

LiChroprep® 

The Guarantee for Selectivity, Reproducibility and Quality 

Selectivity is one of the most important 

factors in chromatography. It 

is decisive in determining the quality 

and the reproducibility of a chromatographic 

separation and, in addition, 

on a preparative scale, it also 

determines, to a large extent, the 

yield. 

In order to be able to guarantee the 

batch-to-batch reproducibility over a 

period of years, it is not only sufficient 

to carry out a simple performance test 

using aromatic substances. We also 

provide comprehensive selectivity 

LiChrospher® for preparative highperformance 

chromatography is a 

spherical and porous silica gel 

characterized by: 

· Uniform and homogeneous silica gel 

matrix from bach-to-batch. 

· Very narrow particle size 

distribution for high performance 

and high packing stability. 

· High permeability at low pressure 

drop in the column. 

· Reproducible specific surface area 

and pore size distribution. 

documentation and carry out an 

additional selectivity test routinely for 

each batch release. 

This selectivity test, which is carried 

out using special solutes under exactly 

defined practice-oriented chromatographic 

conditions, serves to determine 

the smallest change in the selectivity 

properties of the sorbent. 

Merck can therefore guarantee that 

LiChroprep® packing materials will 

produce reproducible results within 

very narrow band ranges. This provides 

the highest reliability for chro- 

LiChrospher® Packing Materials – Sorbents for High-Performance 

Preparative Chromatography 

· Different selectivities (Si 60, RP-18, 

RP-select B and WP 300 RP-18) 

ensure a simple and rapid optimisation 

of the chromatography system 

with respect to selectivity and sample 

composition. 

· Two different particle size ranges 

(12µm and 15µm) ensure the 

necessary chromatographic performance 

to be selected for the desired 

application and thus optimised 

throughput for the chosen selectivity. 

Non-derivatized silica gels still play 

an important role in preparative 

chroma-tography. For routine 

applications, it is important that 

spherical silica gels are produced 

according to the same stringent guidelines 

as irregular Merck silica gels.

matographic separations in 

the laboratory, the pilot 

plant and in full scale 

production. 

The advantages: 

· Identical selectivity of 

analytical and preparative 

material means scale-up 

without compromise. 

· Identical batch-to-batch 

selectivity is the guaranteed 

assurance for 100% 

reproducibility – over a 

period of years. 

This means that only those 

spherical silica gels are released 

for sale that meet 

all required specifications 

with respect to pore structure, 

specific pore volume, 

specific surface area and 

chemical purity as well as 

complying with additional 

important chromatographic 

parameters. 

Independent of the chromatography 

process used to 

develop a particular method, 

the direct and rapid transfer 

from lab to production scale 

is guaranteed. Identical raw 

materials used in spherical 

silica gel manufacturing 

ensures direct scale-up. 

LiChroprep® / Ordering information 

Designation Cat.-No. Particle size Package Contents 

LiChroprep® Si 60 1.09319.0100 5 – 20 µm Plastic 100 g 

LiChroprep® Si 60 

1.09336.1000 

1.09336.9025 

15 – 25 µm Plastic 

1 kg 

25 kg 

LiChroprep® Si 60 1.09390.1000 25 – 40 µm Plastic 1 kg 


1.13905.0250 

1.13905.1000 


250 g 

1 kg 

LiChroprep® Si 100 1.13904.1000 25 – 40 µm Plastic 1 kg 

LiChroprep® RP-2 1.09304.0100 25 – 40 µm Plastic 100 g 

LiChroprep® RP-select B 1.09302.0100 

1.09302.1000 


100 g 

1 kg 



1.09303.0100 100 g 


1.09303.9025 25 kg 

LiChroprep® RP-18 

1.13900.0250 

1.13900.1000 


250 g 

1 kg 

LiChroprep® DIOL 1.13973.0250 40 – 63 µm Plastic 250 g 

LiChroprep® NH2 1.13925.0100 25 – 40 µm Plastic 100 g 

LiChroprep® NH2 1.13974.0250 40 – 63 µm Plastic 250 g 

LiChroprep® CN 1.13959.0250 40 – 63 µm Plastic 250 g 

LiChrospher® packing materials / Ordering information 


LiChrospher® Si 60 

1.19654.0100 

1.19654.1000 

12 µm Plastic 

100 g 

1 kg 


LiChrospher® 100 RP-18 


LiChrospher® 60 RP-select B 


LiChrospher® WP 300 RP-18 

1.11024.0100 100 g 

1.11024.1000 15 µm Plastic 1 kg 

1.11024.9025 25 kg 

1.19656.0100 

100 g 

1.19656.0500 


500 g 

1.11022.0100 100 g 

1.11022.0500 15 µm Plastic 500 g 

1.11022.9025 25 kg 

1.19655.0100 

100 g 

1.19655.0500 


500 g 

1.11023.0100 

1.11023.0500 


100 g 

500 g 

1.19659.0100 

1.19659.0500 


100 g 

500 g 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

29

30 

Other Sorbents: 

Cellulose · Magnesium Silicates · Silica 

Standardized Silica Gels – Surface-Modified and Enantioselective Carriers 

Microcrystalline Cellulose 

This is a hydrophilic polysaccharide 

packing material preferred for the raw 

separation of amino acids and related 

compounds. Cellulose is also used for 

the gentle purification of biomolecules. 

Magnesium Silicates – Florisil® 

Florisil® is a polar highly selective 

magnesium silicate of the approximate 

composition MgO/SiO 2 (15/85) which 

is particularly suitable for the separation 

of steroids, alkaloids, antibiotics 

etc. This stationary phase is also 

used for the sample preparation of 

environmental samples such as pesticide 

residue analysis, chlorinated 

Reversed phase material for production scale 

Because of the organic nature of 

cellulose, it can only be used in a preswollen 

state and under gentle or 

hydrostatic pressure. 

hydrocarbons and pesticides. For 

sample preparation in the case of 

pesticides, a specially purified and 

activated Florisil® is often used (Cat.- 

No. 1.12994). The normal activation 

temperature for Florisil® is 650°C, 

whilst activation at 260°C produces a 

less active material. 

· An alternative material to non-modified silica gel because non-toxic solvents 

in the RP-mode are used. 

· For the economic purification of products where conventional RP-phases 

are too expensive. 

· High loading capacity due to the high specific surface area of the base silica gel 

(60 Å pore diameter, approx. 500 m 2 /g); therefore column dimensions/amount of 

sorbent can be reduced or the sample volume increased. 

· High pressure stability under extreme production conditions 

for long-term column usage. 

Standardized Silica Gels – Modified see table at page 31

Cellulose / Ordering information 


Cellulose microcrystalline 

Avicel® 

Florisil® / Ordering information 

1.02331.0500 20 –100 µm Plastic 500 g 

1.02331.2500 20 –100 µm Plastic 2.5 kg 

1.02331.9025 20 –100 µm Plastic 25 kg 

Specification Florisil® packing materials 

Composition MgO 15,5 % 

SiO2 84.0 

Na2S04 0.5% 

pH approx. 8.5 

SBET approx. 300 m2 /g 

Specific weight approx. 2.51 g/ml 

Porosity approx. 56 % 

Surface acidity (PK.) approx. 1.5 


Florisil® (60-100 mesh ASTM) 1.12518.0100 

100 g 


1.12518.1000 1 kg 

Florisil® for residual analysis 

(60-100 mesh ASTM) 

Standardized silica gels – modified / Ordering information 


Silica gel 60 silanised 

(dimethylsilane derivate) 

(70-230 mesh ASTM) 

1.07719.0250 

1.07719.1000 


250 g 

1 kg 

Silica gel 60 RP-18 

(230 -400 mesh ASTM) 

1.12994.0100 

100 g 


1.12994.1000 1 kg 

1.10167.1000 40 – 63 µm 1 kg 

1.10167.5000 40 – 63 µm Plastic 5 kg 

1.10167.9025 40 – 63 µm 25 kg 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

31

32 

PharmPrep® 

PharmPrep® media are a new line 

of spherical silica sorbents designed 

for process chromatography in the 

pharmaceutical market. 

The first product to be made available, 

PharmPrep® CC for preparative 

chromatography, is a spherical, totally 

porous Silica Gel manufactured by a 

large-scale process from water glass 

and sulphuric acid to produce a large 

particle spherical product. 

The spherical materials have the 

follwing advantages for classical open 

column chromatography: 

Aluminium Oxide for Preparative Chromatography 

Aluminium oxide is one of the most 

versatile sorbents for preparative 

chromatography. Due to its weaker 

adsorption than silica gel, standard 

aluminium oxide can be used in 

specifically defined pH-ranges. 

The aluminium oxide matrix 

comprises octahedral and tetrahedral 

aluminium atoms coupled with 

oxygen, whereby the surface 

aluminium atoms are saturated with 

free hydroxyl groups. The different 

acidic centres (Broenstedt acid, Lewis 

acid and Lewis basic groups) result in 

the fact that, in contact to ionic salt 

solutions, aluminium oxides possess 

both anion and cation exchange 

properties. Its better pH-stability, 

especially in the alkaline range, in 

comparison with silica gels is worth 

mentioning. 

They are easily packed into columns; 

they have high bulk densities, which 

result in good loadability; and they 

exhibit low back pressures, allowing 

for fast elutions. 

PharmPrep® CC can be used in conjunction 

with spherical sorbents for 

performing process chromatography 

at any scale and at a reasonable cost. 

PharmPrep® CC is the ideal media for 

high-quality, high-performance separations 

at a true process scale. 

Aluminium oxide occurs in various 

crystal forms. For chromatography, the 

γ-structure in the form of aluminium 

oxide 90 with a mean pore diameter of 

90 Å is mostly used. Further structural 

types are aluminium oxide 60 (ε-type) 

of 60 Å pore diameter and aluminium 

oxide 150 (α-type) of 150 Å pore 

diameter, each of which possesses 

specific adsorption properties. 

Standardized aluminium oxide 90 for 

chromatographic adsorption analysis 

according to Brockmann is a sorbent 

of medium polarity and it is frequently 

used when the cationic exchange 

properties of the basic aluminium 

oxide are required. In addition, it is an 

alternative material to activated charcoal 

where this product cannot be 

used because of its organic character.

Specification PharmPrep® 

Mean Pore Size (Å) 60 

Pore Volume (ml/g) 0.74 - 0.84 

Specific Surface Area (m2 /g) ~ 500 

Bulk Density (g/ml) ~ 0.55 

Loss on Drying (150°; %) < 2 

PharmPrep® CC 

Aluminium oxide / Ordering information 

Designation 

Aluminium oxide 60 

active, basic 

Aluminium oxide 90 active, basic 

Aluminium oxide 90 active, 

neutral 

Aluminium oxide 90 active, acidic 

Aluminium oxide 90 standardised 

for chromatographic adsorption 

analysis according to Brockmann 

Aluminium oxide 150 basic 

*pH of a 10 % aqueous suspension 

PharmPrep® / Ordering information 

Designation Cat.-No. Particle size Contents 

1.09371.0100 100 g 


1.09371.1000 25 - 40 µm 1 kg 

1.09371.5000 5 kg 

1.09372.0100 100 g 


1.09372.1000 40 - 63 µm 1 kg 

1.09372.5000 5 kg 

1.09373.0100 100 g 


1.09373.1000 63 - 200 µm 1 kg 

1.09373.5000 5 kg 

Spherical, totally porous particles = easy packing of efficient columns without additional apparatus. 

High bulk density = increased loadability, greater throughput and better process 

economics. 

High mechanical stability = high flow rates and low fines generation, minimal need for repacking 

due to particle degradation and frit clogging. 

60 Å pore size = good accessibility for molecules up to 1000 Daltons coupled with high 

surface area for a maximum loadability. 

Fraction eluable 

Cat.-No. Activity pH* with water Chloride Sulfate Content 

1.01067.1000 I 

1 kg 

1.01067.2000 I 

9.0 ± 0.5 < 0.2 % < 0.004 % < 0.1 % 

2 kg 

1.01076.1000 I 1 kg 

1.01076.2000 I 9.0 ± 0.5 < 0.2 % < 0.004 % < 0.1 % 2 kg 

1.01076.9020 I 20 kg 

1.01077.1000 I 1 kg 

1.01077.2000 I 7.3 ± 0.5 < 0.2 % < 0.01 % < 0.1 % 2 kg 

1.01077.9020 I 20 kg 

1.01078.1000 I 1 kg 

1.01078.2000 I 4.0 ± 0.5 < 0.2 % < 0.2 % < 0.1 % 2 kg 

1.01078.9020 I 20 kg 

1.01097.1000 II-III 1 kg 

1.01097.1006 II-III 9.0 ± 0.5 < 0.5 % < 0.1 % < 0.1 % 6 x 1 kg 

1.01097.5000 II-III 5 kg 

1.01061.1000 I-II 

1 kg 

1.01061.2000 I-II 

9.0 ± 0.5 < 0.5 % < 0.004 % < 0.1 % 

2 kg 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

33

34 

Preparative Liquid Chromatography Columns 

Lobar®-Ready-to-Use Glass Columns for Medium-Pressure Chromatography 

Ready-to-use glass columns with a broad range 

of frequently used stationary phases are available 

for convenient medium-pressure (up to 

6 bar) preparative chromatography. Lobar® 

columns are available in various dimensions for 

the purification of compounds up to gram size. 

Reliable LiChroprep® materials with a particle 

size 40 - 63 µm are used as stationary phases. 

Due to the mechanized packing technique used 

Lobar® glass columns have a higher reproducibility 

and excellent performance. They are, of 

course, biocompatible as only materials such as 

Teflon®, ceramic frits and glass are used; thus 

even the most sensitive samples can be purified. 

Due to their simple, reliable and reproducible 

application, Lobar® glass columns have become 

highly acceptable throughout the world, a fact 

that is reflected in numerous publications. 

A literature reference list containing several 

hundred references is available for those who 

are interested in the preparative purification of 

products using Lobar® glass columns and 

LiChroprep® stationary phases. 

Hibar®-Ready-to-Use Pre-Packed Columns (250 – 25 mm) 

If you want the versatility to quickly change 

columns but you prefer to purchase ready-to-use 

columns with the required sorbents, then the 

columns filled with LiChrospher® or LiChroprep® 

provide the perfect answer. 

Although the special engineering know-how 

and construction skills required to manufacture 

preparative HPLC columns often make them 

very expensive, the ready-to-use column enables 

you to purchase high-quality, ready-to-use columns 

at a very reasonable price. 

Thus, if you are looking for the highest quality 

standards without stressing your budget, 

then Hibar® columns provide the answer. 

Benefits of Lobar® pre-packed columns: 

· All parts that come into contact with liquid 

are biocompatible 

· Lobar® columns are available in various 

dimensions 

· Lobar® columns guarantee high reproducibility 

and performance 

Specification of Lobar ® glass columns 

Column Internal Sample Contents of 

length Diameter amount stationary phase 

[mm] [mm] [g] [mL] [g] 

240 10 < 0.2 < 1 ~ 20 

310 25 < 1 < 5 ~ 70 

440 37 < 3 < 10 ~ 220 

Benefits of Hibar® pre-packed columns: 

Quality Hibar® pre-packed columns are manufactured 

to strictly controlled conditions to 

ensure both excellent results and reproducibility. 

Each packed column is provided 

with a Certificate of Analysis. 

Versatile You can choose your Hibar® pre-packed 

column from an extensive range of 

sorbents. 

Easy All columns are ready-to-use and can be 

easily connected to any HPLC system. 

Problem-free Methods developed on a particular sorbent 

on an analytical scale can be directly 

scaled-up to preparative scale.

Lobar®-ready-to-use column / Ordering information 

Designation Cat.-No. Stationary phase [g] Dimension [Length] i.d. Contents 

LiChroprep® Si 60 1.10400.0001 ~ 20 240 mm 10 mm 1 piece 



LiChroprep® RP-18 1.10624.0001 ~ 20 240 mm 10 mm 1 piece 



Accessories for Lobar ® -ready-to-use glass columns 

Designation Ordering No. Contents of one package 

Lobar® glass columns basic equipment 1.10407.0001 5 m PTFE capillary tube (o.d. 1.5 mm, i.d. 1 mm) 

1 glass capillary 3-way valve and Teflon stopcock (4) 

1 glass syringe with Luer® connection (2 ml) 

4 stainless steel cannulas (o.d. 1.5 mm) 

3 stainless steel connecting capillaries (o.d. 1.5 mm, i.d. 1 mm) 

1 pack emery paper (for holding the tubing) 

1 awl (for PTFE tubes) 

Lobar® assembly kit 1.15395.0001 2 adaptors for tube conection (o.d. 1/16“) (PTFE b ody with PP nut) for direct 

connection to the Lobar® column 

5 m PTFE tubing (o.d. 1/16“, i.d. 0.8 mm) 

1 dead-volume free coupling unit with 2 nuts (PP) 

4 PTFE gripper with stainless steel retainer 

1 PTFE connector for tubing connection (o.d. 1/16“ or 1/8“) complete with 

fluoron seals and 2 PP nuts) 

2 PTFE cones for PTFE connector 

6 spare O-rings 

^ 

Lobar® connectors (dead-volume free) 1.15455.0001 5 connectors with 10 nuts for tubing connector (o.d. 1/16“) (PP) 

Lobar® PTFE gripper with stainless steel retainer 1.15396.0001 10 PTFE gripper with stainless steel retainer for tubing connector (o.d. 1/16“) 

Lobar® nuts 1.15393.0001 10 nuts for tubing connection (o.d. 1/16“) (PP) 

Prepacked Hibar® 

column (250 - 25 mm) 

Hibar® pre-packed columns (25 mm, i.d.), available 

ex-stock / Ordering information 

Cat.-No. Particle Dimension Content 

size [µm] [mm] 

LiChrosorb® Si 60 1.51435.0001 7 250 - 25 1 piece 

LiChrosorb® RP-18 1.51494.0001 7 250 - 25 1 piece 

LiChrosorb® RP-8 1.51441.0001 7 250 - 25 1 piece 

LiChrosorb® RP-select B 1.51464.0001 7 250 - 25 1 piece 

LiChrospher® Si 60 1.51485.0001 5 250 - 25 1 piece 

LiChrospher® 100 RP-18 1.51483.0001 5 250 - 25 1 piece 

LiChrospher® 100 RP-8 1.51482.0001 5 250 - 25 1 piece 

LiChrospher® 60 RP-select B 1.51484.0001 5 250 - 25 1 piece 

Replacement frits for Hibar® columns 1.51221.0001 1 piece 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

35

36 

Customized Preparative Columns (25 and 50 mm, i.d.) of Higher Quality 

We offer a set of Hibar® pre-packed, customized 

stainless steel columns (20 and 50 mm, i.d.) for 

high resolution lab-scale purification. 

For your laboratory work, it is not enough just to 

have a top-quality preparative column; you also 

need a method that is and will remain, reliable 

for years. 

Customer benefits at a glance: 

Column Packing Stands NW25, NW50 and NW100: Innovative Column Packing Technology 

Conventional packing methods for preparative 

columns use rapid filtration under pressure and 

at high linear flow rates. This technology often 

leads to bridging effects between the particles in 

the stationary phase that have a negative impact 

on the column performance. Our studies have 

shown that this undesirable side-effect can be 

overcome by employing a pulling instead of a 

pushing mechanism. 

The new column packing technology is based on 

a combination of vacuum suction with subsequent 

column bed compression; this stabilizes 

the packing. A common water-jet pump is used 

to generate the vacuum. The gel slurry is sucked 

through a packing reservoir into the column by 

applying low pressure at the bottom end of the 

column. 

This method allows precise adjustment of the desired 

packing volume or gel bed height. In the 

production environment, the new technology 

You also need to be able to transfer it to other 

laboratories throughout your parent or subsidary 

companies in the world. 

Our own long-term experience has shown how 

important chromatographic procedures are and 

we would like to pass on the benefits of this experience 

to you in the form of a column with 

higher quality and reproducibility. 

Quality: Hibar®-customized pre-packed columns are manufactured to strictly 

controlled conditions to ensure both excellent packing quality and 

reproducibility of a separation. 

Economic: The fully controlled gel bed compression of the 50mm (i.d.) column leads to a 

longer lifespan of the columns and improves process economy. In addition, 

with the hydraulic stand accessory, it can be upgraded to Packing Stand NW50. 

Versatile: You can choose your packing materials (Merck or those of other manufacturers) 

for the Hibar®-column (25 or 50mm, id) from an extensive range of 

sorbents. 

Simple: Columns are ready-to-use and can be easily connected with any HPLC system. 

Problem-free: Methods developed on a particular sorbent on an analytical scale can be 

directly scaled-up to preparative scale. 

facilitates the determination of the column geometry 

and thereby makes direct scale-up from 

analytical to preparative scale easy. 

The new column packing devices are available 

for columns of 25 mm, 50 mm and 100 mm inner 

diameters, covering the full range from semi-preparative 

to pilot-scale and production scale. 

Packing stands 

NW25, NW50 and 

NW100 with 

temperature 

control jacket 

(option)

Ordering information about customised packing 

Designation Cat.-No. Dimensions i.d. Contents of one package 

Special packing for Hibar® pre-packed column* 1.50004.0001 250 - 25 1 piece 

Special packing for Hibar® pre-packed column* 1.50016.0001 125 - 25 1 piece 

Special packing for Hibar® packed column* 1.50099.0001 250 - 50** 1 piece 

Sorbent for customised packing 

Sorbent of Merck Modification Pore size [Å] Particle size [µm] 

LiChrospher® Silica and RP-select B 60 5, 10 and 12 

LiChrospher® RP-select B 60 7 

LiChrospher® RP-18 60 10 

LiChrospher® Silica and RP-18, 100 5, 7, 10, and 12 

LiChrospher® RP-18 E 100 5 and 10 

Purospher® RP-8 and RP-18 100 5 and 10 

LiChrospher® RP-8 100 5, 7 and 10 

LiChrospher® CN, NH 2 and DIOL 100 5 and10 

LiChrospher® RP-18 Wide pore 300 5, 12 and 15 

LiChrosorb® Silica 60 5, 7 and 10 

LiChrosorb® RP-select B 60 5, 7, 10 and 12 

LiChrosorb® Silica, RP-8, RP-18, CN, NH 2 and DIOL 100 5, 7 and 10 

LiChrosorb® Silica and RP-18 100 12 and 15 

LiChroprep® Silica 60 5-20, 15-25, 25-40 and 40-63 

LiChroprep® Silica and RP-2 100 25-40 

LiChroprep® Silica, RP-8 and RP-18 100 5-20, 15-25, 25-40 and 40-63 

LiChroprep® CN, NH 2 and DIOL 100 25-40 and 40-63 

Chiral separation 

Welk-01 R,R and S,S 12 

Specification – Customised columns, packed with the sorbents from other manufacturers available on request 

Packing stands NW25 / Ordering information 

Designation Cat.-No. Dimensions Length Dimensions i.d. Contents of one package 

Packing stand NW25 complete with hydraulics stand 1.25940. 0001 260 mm 25 mm 1 

Accessories for Packing stand NW25 

Complete column tube without hydraulics 1.25941. 0001 260 mm 25 mm 1 

Complete column tube without hydraulics 

Complete column tube with temperature control jacket 

1.25942. 0001 155 mm (short version) 25 mm 1 

without hydraulics 

Hydraulics stand for column tube NW25 

1.25950. 0001 260 mm 25 mm 1 

complete plus filling tube 1.25946.0001 1 

Gasket Set (outlet cover plate) 1.25014. 0001 1* Fritt 

1* Teflon ring 

PEEK Connecting Set for Packing stand NW25 1.25914. 0001 1 m PEEK 

capillery 1/8" 

1*male nur 1/8" 

1*ferrule 1/8" 

Fitting Set for Packing stand NW25 1.25005. 0001 5 male nuts 1/8" 

10 ferrules 1/8" 

5 capillary 

connectors 1/16 

to 1/8" 

Frit holder for compression piston for Packing stand NW25 1.25010.0001 1 

Tripod for Packing stand NW25 and NW50 1.25961.0001 1 

Sealing ring for column NW25 1.25013.0001 2 

NW50 and NW100 see page 38 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

37

38 



Complete Packing stand NW50 top flange 

with screw seal, incl. hydraulics stand 

1.25911. 0001 250 mm 50 mm 1 

Complete Packing stand NW50 with top flange 

chain bolted , incl. hydraulics stand 

1.25931.0001 250 mm 50 mm 1 


Frit holder for compression piston NW50 1.25908. 0001 1 

Sealing ring 1.25909. 0001 2 

Gasket Set (outlet cover plate) 1.25913. 0001 1*Fritt 

1*Teflon ring 

1*Distribution plate 

PEEK connecting Set for Packing stands 1.25914. 0001 1m PEEK capillery 1/8" 

NW 25 and NW 50 1 male nur 1/8" 

1 ferrule 1/8" 

Complete column tube top flange 

with screw seal without hydraulics 

1.25912. 0001 250 mm 50 mm 1 

Complete column tube with top flange chain 

bolted without hydraulics 

1.25932. 0001 250 mm 50 mm 1 

Complete column tube top flange 1.25943. 0001 155 mm 50 mm 1 

with screw seal without hydraulics stand (short version) 


and top flange chain bolted without hydraulics stand 

1. 25949. 0001 250 mm 50 mm 1 


and top flange with screw seal without hydraulics stand 

1.25948. 0001 250 mm 50 mm 1 

Hydraulics stand complete (for column tube NW 50 

top flange with screw seal plus filling tube) 

1.25945. 0001 1 

Hydraulics stand complete (for column tube NW 50 

with top flange chain bolted plus filling tube ) 

1.25947. 0001 1 

Fitting Set for Packing stand NW 25 and 50) 1.25005.0001 5 male nuts 1/8" 

10 ferrules 1/8" 

5 capillary connectors 

1/16 to 1/8" 

Tripod for Packing stand NW25 and NW50 1.25961.0001 1 



Complete Packing stand NW100 top flange 

with screw seal, incl. Hydraulics stand 

1.25930. 0001 400 mm 100 mm 1 

Complete Packing stand NW 100 with top flange 

chain bolted incl. Hydraulics stand 

1.25936. 0001 400 mm 100 mm 1 


Complete column tube 

top flange with screw seal without hydraulics 

1.25951.0001 400 mm 100 mm 1 


with top flange chain bolted without hydraulics 

1.25952.0001 400 mm 100 mm 1 

Complete column tube 1.25955.0001 300 mm 100 mm 1 

top flange with screw seal without hydraulics (short version) 


with top flange chain bolted without hydraulics 

1.25956.0001 300 mm 100 mm 1 


and top flange chain bolted without hydraulics 

1.25957.0001 300 mm 100 mm 1 


and top flange with screw seal without hydraulics 

1.25953.0001 400 mm 100 mm 1 



1.25954.0001 400 mm 100 mm 1 

Hydraulics stand complete for column tube NW100 

(top flange with screw seal plus filling tube and basic stand 

1.25958.0001 1 

Hydraulics stand complete (for column tube NW 100 with 

top flange chain bolted plus filling tube and basic stand) 

1.25959.0001 1 

Gasket Set (upper outlet cover plate) 1.25937.0001 1 Fritt 

1 Teflon ring 

1 Distribution plate 

Teflon lined flexible stainless steel tube (1.5m*1/4") Set 1.25938.0001 consisting of: 1.5m PTFE 

lined stainless steel tube 1/4" 

1 male nut 1/4" 

1 ferrule 1/4" 

Sealing ring for piston NW 100 1.25935.0001 2 

Frit holder for compression piston NW100 1.25934.0001 1 

O-ring for new frit holder NW100 1.25960.0001 5

Packing and Unpacking the Packing Stands 

The required volume of gel slurry is prepared 

according to the physical parameters of the 

stationary phase and the column size. Using a 

funnel, the suspended material is filled into the 

packing tube attached to the column. Vacuum is 

applied. After the suspension has been sucked 

into the column tube, the packing tube is 

removed and the upper column cap lid (flange) is 

sealed. The column bed is then washed with 

3-5 column volumes of a suitable 

solvent and simultaneously 

compressed over several 

steps to a bed compression that 

depends on the sorbent type 

being used (see operating 

instructions). In this way, rapid 

Customer benefits at a glance: 

· The new vacuum packing technology permits simple and flexible handling 

of preparative columns. 

· The separation columns can be prepared rapidly on-site. 

sedimentation and homogeneous packing of the 

column is achieved. The column is ready for 

operation. 

To unpack the column, the upper flange lid is 

removed and the material is forced out by 

moving the piston upwards with the hydraulic 

pump. The column is now ready for the next 

filling procedure. The stationary phase may be 

kept for later use or disposed of. 

· The fully controlled gel bed compression leads to a longer lifespan of the columns 

and improved process economy. 

· Even soft gels such as polymer-based gels can be packed with the new packing devices. 

· One single packing stand can be used to pack an unlimited number of columns 

of the same inner diameter. 

· You can choose between an economic upper screw cap and the convenient 

chain-closing system for the 50 and 100mm ID packing stands. 

· Customized column lengths can be provided on request. 

· Columns with temperature control jackets provide optimum separation 

stability and performance (optional). 

· Optimum price and performance. 

Packing of a column 

slurry 

solvent 

Column 

packing 

Solvent 

outlett (to collection) 

Sample 

purification 

Solvent 

inlett 

(to pump) 

Sample 

unpacking 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

39

Mesopores 

Macropores 

40 


Increase in speed, efficiency and productivity 

Chromolith® Prep monolithic stationary phases are 

new ultra-pure silica phases that have special 

properties resulting from their bimodal pore 

structure. This structure is based on new “Sol- 

Gel” technology and consists of macropores and 

mesopores. 

The mesopores, with an average diameter of 

12 nm, form the fine porous structure of the 

column interior and create a very large surface 

area on which adsorption of the target compounds 

occurs. 

The large macropores, with a pore diameter 

ranging from 3 µm, form a dense network of 

pores and allow their high flow rate due to their 

low resistance factor. The resulting excellent 

accessibility of the mesopores (total porosity 

Different application with Chromolith® Prep Si (100-25mm) 

Separation at high flow rate 

Sample: Toluene, Dimethylphthalate, 

Dibutylphthalate 

Column : Chromolith® Prep (100-25 mm) 

Solvent: n-heptane/dioxane ( 80/20 v/v) 

Flow rate 40 ml/min 

Separation at different flow rates 40 and 390 ml/min 

Flow rate 390 ml/min 

> 80 %) ensures fast adsorption and desorption 

kinetics because of the short diffusion length 

inside the pores. This results in dramatically 

reduced separation times and thus in a huge 

increase in productivity. 

To operate a monolithic column it is necessary 

to ensure that no flow-through occurs in the 

wall region. Therefore, the monolith is cladded 

with a polymeric material (special PEEK) and can 

be connected directly to any HPLC system and 

used as a ready-to-use-column. 

Specification of Chromolith® Prep Si 

Macropores [µm] 3 

Mesopores [nm] 12 

Specific pore volume [ml/g] 1 

Specific surface area [m2/g] 350 

Packing density [g/ml] 0.2 

Total porosity 0.8 

Surface pH neutral 

Iron [%] content no 

Chromolith® Prep 

columns can be 

operated at a 

flow rate of up 

to 400 ml/min, 

which is a tenfold 

increase to that 

of a comparable 

column packed 

with a non-monolithic 

or a particulate 

sorbent.

Separation of χ- and δ-Tocopherol from sunflower oil at different flow rates 

Flow rate 40 ml/min Flow rate 160 ml/min 

Separation Diasteriomers with a productivity of 861g/d 

Column: Chromolith® Prep Si (100-25mm) 

Flow rate: 140 ml/min 

Injection: 249 mg 

Cycle Time: 25 sec. 

Sample: Fluoro , dihydro- oxiranyl-benzopyran 

Chromolith® Prep Si (100-25mm) / Ordering information 

Packing material Cat.-No. Package 

Chromolith® Prep Si (100-250mm) 1.25251.0001 1 piece 

Chromolith® Prep RP 18 E (100-25mm) 1.25252.0001 1 piece 

Filter for Chromolith® Prep Si 1.25253.0001 10 pieces 


before cladding 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

41

42 

Contract Separation at Merck 

Until recently in the pharmaceutical industry, the 

splitting up of project work was only possible if 

kept within the company. However, increasing 

costs and the fact that development times for 

new products are extending over 10 to 15 years 

has forced companies into new, innovative 

thinking. The principle of “do everything yourself” 

is no longer meaningful in many areas, e.g. 

in the case of chromatographic separations: the 

considerable investment necessary for HPLC 

equipment and the need for dedicated buildings 

is a substantial risk bearing in mind that the 

products in question still have to undergo phase 

I and III clinical trials - and may not even be 

approved for marketing at the end of the day. 

Hence, “out-sourcing” – the sharing of work 

with an external partner – is a meaningful 

strategy pursued successfully by many companies 

today in meeting this challenge. Chromatographic 

separation problems in particular 

can be ideally solved in this way if a suitable 

out-sourcing partner can be found. The risks can 

be substantially reduced and the costs kept to an 

acceptable and controllable level. 

Merck can provide a complete solution from one 

source: apart from our comprehensive range of 

chromatography products, we also have a contract 

separation service available for our customers. 

We provide know-how, experience, competence 

and “capacity“ in two ways: 

Merck is one of the largest production and sales 

organizations for chromatography equipment 

and sorbents and has accumulated extensive 

know-how over many years in all areas of chromatography 

– from analytical to preparative and 

to production scale. Our highly qualified 

specialists on the one hand and the stringent 

maintenance of relevant standards (DIN ISO 

9001, FDA, GMP, GLP etc.) on the other enable 

us to tackle and solve any chromatographic 

problem and to develop economic processes for 

the production of amounts ranging from 

milligrams to tons. 

Each and every employee involved with your 

problem is a “capacity” in his specialized area. 

And, due to our sheer size and infrastructure, we 

can provide the downstream processing capacity 

that you could hardly ever hope to achieve on 

your own. 

Instrumentation for contract separation: 

· Analytical and semi-preparative HPLC equipment 

(LaChrom® and NovaPrep®) 

· HPLC production plant (Prepbar® 200 and 

Prepbar® 300) with appropriate solvent 

distillation equipment 

· SMB plant for continuous chromatography 

Substance classes that can be separated: 

·Purification of natural substances (of plant, 

bacterial and fungal origin) 

· Synthetic pharmaceutical products 

· Plant extracts 

·Peptides 

· Racemates 

· Fine chemicals 

- You save time, money and capacity. 

Step by step in partnership: the route to success 

Merck has been a successful service company 

for the pharmaceutical industry for many years, 

both at home and abroad. We can offer you a 

fully customized solution for your separation 

problem. 

Why not test us? 

Just call or fax - it may just be the step that 

could help your company to minimize risk. For 

you, there is absolutely no risk - a secrecy 

agreement will keep everything confidential 

right from the beginning. 

Would you like further information? 

We will be glad to make an appointment, even at 

short notice, for a personal discussion 

of your project. 

Just call (+49 6151 72 6177) or 

fax us (+49 6151 72 91 6177). 

We will reply by return.

Biochemicals for Processing 

Merck supplies an extensive range of reagents 

and fine chemicals with well defined properties 

for use in biotechnology, molecular biology and 

analysis. It is of crucial importance for the quality 

grades later required in manufacturing to be already 

available on a research and pilot scale in 

order to facilitate the easy up-scaling of the 

processes. 

In the list below you will find a comprehensive 

product selection from our main catalogue 

dedicated to “processing” purposes. The biochemicals 

are listed in alphabetical order. 

Product Cat.-No. Pack size 

Albumin fraction V (from bovine serum) for biochemistry 

Ammonium sulfate for biochemistry 

Brij® 35 solution (30 % w/w in water) for biochemistry 

Cacodylic acid sodium salt trihydrate buffer substance for biochemistry 

Cellobiose for biochemistry 

CHAPS (3-[(3-Cholamidopropyl)-dimethylammonio]-propane-sulfonate) 


Creatinine for biochemistry 

α-Cyclodextrine for biochemistry 

Deoxyribunucleic acid (from herring sperm) sonicated for meolecular biology 

Dihydronicotinamide adenine dinucleotide disodium salt for biochemistry, NADH-Na 2 

Dihydronicotinamide adenine dinucleotide phosphate tetrasodium salt 

for biochemistry, NADPH-Na 4 

5-Dimethylaminonaphthalene-1-sulfonyl chloride (Dansylchloride) for biochemistry 

3-(4,5-Dimethyl-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide for biochemistry 

2,2-Dinitro-5,5-dithiodibenzoic acid for biochemistry 

1,4-Dithioerythritol for biochemistry 

1,4-Dithiothreitol for biochemistry 

Ethylene glycol monomethyl ether for amino acid analysis 

Folic acid for biochemistry 

D(-)-Fructose for biochemistry 

D(+)-Galactose for biochemistry 

D(+)-Glucose anhydrous for biochemistry 

D(+)-Glucose monohydrate for biochemistry 

1.12018.0005 

1.12018.0025 

1.12018.0100 

1.12018.0500 

1.01211.0250 

1.01211.1000 

1.01211.5000 

1.01211.9050 

1.01894.0100 

1.01894.1000 

1.03256.0010 

1.03256.0100 

1.02352.0025 

1.11662.0001 

1.11662.0010 

1.05206.0050 

1.05206.0500 

1.02126.0050 

1.24711.0010 

1.24644.0005 

1.24540.0050 

1.24540.0250 

1.03094.0001 

1.03094.0010 

1.11714.0001 

1.11714.0010 

1.03291.0001 

1.03291.0005 

1.03291.0025 

1.24511.0001 

1.24511.0005 

1.24511.0025 

1.11474.0001 

1.11474.0005 

1.11474.0025 

1.15118.1000 

1.15118.2500 

1.15118.9026 

1.03984.0005 

1.03984.0025 

1.04007.1000 

1.04058.0025 

1.04058.0100 

1.08337.0250 

1.08337.1000 

1.08337.5000 

1.08337.9050 

1.04074.1000 

1.04074.5000 

1.04074.9050 

5 g 

25 g 

100 g 

500 g 

250 g 

1 kg 

5 kg 

50 kg 

100 ml 

1 l 

10 g 

100 g 

25 g 

1 g 

10 g 

50 g 

500 g 

50 g 

10 mg 

5 g 

50 mg 

250 mg 

1 g 

10 g 

1 g 

10 g 

1 g 

5 g 

25 g 

1 g 

5 g 

25 g 

1 g 

5 g 

25 g 

1 l 

2.5 l 

25 l 

5 g 

25 g 

1 kg 

25 g 

100 g 

250 g 

1 kg 

5 kg 

50 kg 

1 kg 

5 kg 

50 kg 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

43

44 


Glutathione (reduced) for biochemistry 

Glycerol triacetate for biochemistry 

Glycerol triacetate Ph Eur, BP, USP 

Guanidinium chloride for biochemistry 

Guanidinium chloride LAB 

Guanidinium thiocyanate for biochemistry 

Indole-3-acetic acid for biochemistry 

Indole-3-butyric acid for biochemistry 

myo-Inositol for biochemistry 

Lactose monohydrate for biochemistry 

Lithiumdodecylsulfat for biochemistry 

Lithium hydroxide monohydrate for amino acid analysis 

DL-Malic acid for biochemistry 

L(-)-Malic acid for biochemistry 

Maltose monohydrate for biochemistry 

D(+)-Mannose for biochemistry 

Melibiose monohydrate for biochemistry 

1-Naphthylacetic acid for biochemistry 

Nicotinamide adenine dinucleotide free acid for biochemistry, NAD 

Nicotinamide adenine dinucleotide phosphate disodium salt 

for biochemistry, NADP-Na2 Octanoic acid for biochemistry 

2-Oxoglutaric acid for biochemistry 

PefablocR SC (4-(2-Aminoethyl)-benzolsulfonylfluorid-hydrochloride) (AEBSF) 


Phthaldialdehyde for fluorometry 

Protamine sulfate for biochemistry 

Pyruvic acid sodium salt for biochemistry 

Raffinose pentahydrate for biochemistry 

1.04090.0001 

1.04090.0005 

1.04090.0050 

1.04090.0500 

1.08238.0500 

1.03000.1000 

1.03000.9010 

1.04219.0025 

1.04219.0100 

1.04219.0500 

1.04220.0100 

1.04220.1000 

1.04220.5000 

1.04220.9010 

1.04220.9025 

1.04167.0025 

1.04167.0250 

1.00353.0010 

1.00353.0100 

1.00353.1000 

1.00354.0005 

1.00354.0025 

1.00354.0100 

1.00354.0500 

1.04507.0050 

1.04507.0250 

1.07660.0250 

1.07660.1000 

1.07660.9010 

1.14243.0010 

1.14243.0050 

1.11652.0250 

1.11652.1000 

1.00382.0250 

1.00382.1000 

1.00381.0005 

1.00381.0025 

1.00381.0100 

1.05912.0025 

1.05912.0100 

1.05388.0025 

1.05388.0100 

1.12240.0005 

1.12240.0025 

1.06220.0010 

1.06220.0100 

1.24542.0001 

1.24542.0005 

1.24542.0025 

1.24541.0001 

1.00193.0250 

1.00193.2500 

1.00193.9025 

1.05194.0025 

1.05194.0100 

1.05194.1000 

1.24839.0100 

1.12839.0500 

1.11452.0005 

1.11452.0050 

1.10123.0005 

1.10123.0025 

1.06619.0050 

1.06619.0250 

1.06619.1000 

1.07419.0050 

1 g 

5 g 

50 g 

500 g 

500 ml 

1 l 

10 l 

25 g 

100 g 

500 g 

100 g 

1 kg 

5 kg 

10 kg 

25 kg 

25 g 

250 g 

10 g 

100 g 

1 kg 

5 g 

25 g 

100 g 

500 g 

50 g 

250 g 

250 g 

1 kg 

10 kg 

10 g 

50 g 

250 g 

1 kg 

250 g 

1 kg 

5 g 

25 g 

100 g 

25 g 

100 g 

25 g 

100 g 

5 g 

25 g 

10 g 

100 g 

1 g 

5 g 

25 g 

1 g 

250 ml 

2.5 l 

23 l 

25 g 

100 g 

1 kg 

100 mg 

500 mg 

5 g 

50 g 

5 g 

25 g 

50 g 

250 g 

1 kg 

50 g


L(+)-Rhamnose monohydrate for biochemistry 

D(-)-Ribose for biochemistry 

tri-Sodium citrate dihydrate buffer substance for amino acid analysis 

D(-)-Sorbitol 

Starch (from wheat) for biochemistry 

Starch soluble GR for analysis, ISO 

Starch soluble acc. to Zulkowsky GR for analysis 

Sucrose (saccharose) for biochemistry 

Sucrose (saccharose) for density gradient ultracentrifugation 

Titanium(III) chloride solution about 15% (in about 10% hydrochloric acid) 

for amino acid analysis 

Trehalose for biochemistry 

Triethanolamine Ph Eur 

Triethanolamine GR for analysis 

Triethanolamine hydrochloride GR for analysis 

Urea for biochemistry 

Uric acid for biochemistry 

D(+)-Xylose for biochemistry 

Vitamin B12 for biochemistry 

For more information, products and qualities please ask for 

the Merck catalogue for Chemicals & Reagents or the Merck 

ChemDAT®. We also welcome you on our homepage 


1.07361.0010 

1.07361.0025 

1.07605.0050 

1.06430.1000 

1.07759.0100 

1.07759.0500 

1.07759.5000 

1.11685.0250 

1.11685.1000 

1.11685.9025 

1.01252.0100 

1.01252.0250 

1.01252.1000 

1.01252.9025 

1.01257.0050 

1.01257.0250 

1.01257.1000 

1.01257.9025 

1.07687.0250 

1.07687.1000 

1.07687.5000 

1.07654.1000 

1.07654.5000 

1.10789.0001 

1.08216.0010 

1.08216.0025 

1.08216.0100 

1.08216.5000 

1.08372.1000 

1.08372.5000 

1.08372.9025 

1.08379.0250 

1.08379.1000 

1.08379.9026 

1.08357.0100 

1.08357.1000 

1.08357.9025 

1.08488.0250 

1.08488.1000 

1.08488.5000 

1.08488.9010 

1.08488.9025 

1.00817.0025 

1.00817.0100 

1.08689.0025 

1.08689.0100 

1.24592.0100 

10 g 

25 g 

50 g 

1 kg 

100 g 

500 g 

5 kg 

250 g 

1 kg 

25 kg 

100 g 

250 g 

1 kg 

25 kg 

50 g 

250 g 

1 kg 

25 kg 

250 g 

1 kg 

5 kg 

1 kg 

5 kg 

6x7.5 ml 

10 g 

25 g 

100 g 

5 kg 

1 l 

5 l 

25 l 

250 ml 

1 l 

25 l 

100 g 

1 kg 

25 kg 

250 g 

1 kg 

5 kg 

10 kg 

25 kg 

25 g 

100 g 

25 g 

100 g 

100 mg 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

45

46 

Enzymes for Processing 

Below you will find a selection of 

Merck enzymes for biochemistry 

mainly used in downstream processes, 

biochemical research and food 

analysis. 

The product description of each enzyme 

gives information on the origin 

of the product, enzyme activity and the 

numerical classification (EC number). 

Definitions of enzyme activity are 

often historical in nature and relatively 

arbitrarily selected. As long as there 

are no standard procedures and very 

few internationally recognized ones, 

one has to be most careful when comparing 

enzyme qualities. Initially, it 

β-Glucoronidase 

This preparation is used for the gentle hydrolysis of 

steroid conjugates, e.g. in the assay of urinary steroids 

and mucopolysaccharides. 

β-Glucoronidase/Arylsulfatase (from Helix pomatia) 

stabilized aqueous solution for biochemistry, 

EC 3.2.1.31 + EC 3.1.6.1. (Merck Cat.-No. 1.04114) 

Lysozyme 

Lysozyme is a mucolytic enzyme. It is used for 

bacteriolysis. 

Lysozyme catalyzes the hydrolysis of β-(1-4) bonds 

between N-acetylmuramic acid and 2-acetamido-2deoxy-D-glucose 

residues in mucopolysaccharides, 

mucopeptides and chitin. 

Lysozyme also gently breaks up bacterial cell walls, e.g. 

of micrococcus luteus or micrococcus lysodeicticus; in 

the presence of EDTA e.g. also of bacillus subtilis and 

escherichia coli. 

Lysozyme (from egg white) 100,000 U/mg cryst. 

(hydrochloride) for biochemistry, EC 3.2.1.17 

(Merck Cat.-No. 1.05281) 

Pepsin 

Pepsin is any one of the small family of closely related 

aspartic proteinase enzymes. 

Pepsin is used for the hydrolysis of peptides and 

proteins, e.g. insulin. 

Preferential cleavage sites are Phe- and Leu-. 

Pepsin only attacks the peptide bonds; esters and 

amides are not hydrolyzed. 

Furthermore, the enzyme can be used for the pepsincatalyzed 

synthesis of oligopeptides. 

Pepsin (from porcine gastric mucosa) 0.7 FIP-U/mg for 

biochemistry, EC 3.4.23.1 (Merck Cat.-No. 1.07185) 

Pepsin (from porcine gastric mucosa) cryst. lyophilized 

10 FIP-U/mg for biochemistry, EC 3.4.23.1 


should be checked whether enzymes 

of identical origin are involved (e.g. 

plant cells, animal origin). Preparations 

from different manufacturers 

can be only compared if their activities 

have been determined under 

identical conditions. A few degrees 

of reaction temperature, different 

buffer systems, pH, different enzyme 

substrates or different reaction times 

make exact comparison practically 

impossible. Differently determined 

units can rarely be converted to each 

other. If a comparison is necessary, the 

only way to do so is by experiment. 

Pronase E 

Pronase E may be used for the gentle hydrolysis of 

proteins or polypetides. 

Compared with acid total hydrolysis, the enzymatic 

splitting amounts to approximately 70 -90 %, due to 

the very low substrate specificity of pronase E. 

The enzyme is also used for the isolation of amino-acidglycoside 

components from mucoproteins. 

Pronase E (from streptomyces griseus) 4,000,000 PU/g 

for biochemistry, EC 3.4.24.4 (Merck Cat.-No. 1.07433) 

Trypsin 

Trypsin is a serine endopeptidase which catalyzes the 

hydrolytic cleavage of proteins and peptides specifically 

at the carboxyl groups of basic amino acids L-Arginine 

and L-Lysine. 

Furthermore, esters and amide bonds of synthetic 

derivatives of these amino acids, e.g. N α-benzoyl-Larginine 

ethyl ester, are also hydrolyzed. 

Trypsin (from porcine pancreas), cryst. lyophilized 

40 U/mg for biochemistry, EC 3.4.21.4 


Trypsin (from porcine pancreas), TPCK-treated, 

lyophilized 40 U/mg for biochemistry, EC 3.4.21.4 


Further enzymes are included in the 

alphabetical list


Aldehyde dehydrogenase (from yeast) lyophilized 100 U/vial 

for biochemistry, EC 1.2.1.5 

α-Amylase (from porcine pancreas) lyophilized 30,000 U/glass for biochemistry 

β-Amylase (from barley malt) lyophilized 28 U/mg for biochemistry, EC 3.2.1.1 

Bromelain (from pineapples) 2 mAnson-U/mg for biochemistry, EC 3.4.22.32 

Catalase (from bovine liver) crystalline suspension in water 15,000,000 U/vial, 

stabilized with 0.01% alkylbenzyldimethylammoniumchloride 


Cellulase Onozuka R-10 (from trichoderma viride) 1 U/mg 


Cholinesterase (from horse serum) lyophilized 500 U/vial 


α-Chymotrypsin (from bovine pancreas) cryst. lyophilized 350 U/mg 


Diastase (from fungi) for biochemistry 

β-Glucoronidase/Arylsulfatase (from helix pomatia) stabilized aqueous solution 

for biochemistry, EC 3.2.1.31 + EC 3.1.6.1 

Glucose dehydrogenase (from bacillus megaterium recombinant) 

lyophilized 250 U/vial for biochemistry, EC 1.1.1.47 

Lysozyme (from egg white) 50,000 U/mg cryst. (hydrochloride) 


Papain (from carica papaya) water-soluble 30,000 USP-U/mg 


Pepsin (from porcine gastric mucosa) 0.7 FIP-U/mg for biochemistry, EC 3.4.23.1 

Pepsin (from porcine gastric mucosa) cryst. lyophilized 10 FIP-U/mg 


Peroxidase (from horse radish) 10,000 U/vial for biochemistry, EC 1.11.1.7 

Pronase E (from streptomyces griseus) 4,000,000 PU/g for biochemistry, 

EC 3.4.24.4 

Thrombin (from bovine plasma) 50 NIH-U/mg for biochemistry, EC 3.4.21.5 

Trypsin (pancreas protease), 200 FIP-U/g, EC 3.4.21.4 

Trypsin (from porcine pancreas) cryst. lyophilized 40 U/mg for biochemistry, 

EC 3.4.21.4 

Trypsin (from porcine pancreas) lyophilized, purified by affinity chromatography 

for protein sequence analysis, EC 3.4.21.4 

Trypsin (from porcine pancreas), TPCK-treated lyophilized 40 U/mg 


Urease (from jack beans) lyophilized 5 U/mg for the determination of urea in serum, 

EC 3.5.1.5 

Urease (from jack beans) solution in 50% glycerol, 1000 U/ml 


1.15640.0001 

1.16312.0001 

1.01327.0001 

1.01651.0025 

1.01651.1000 

1.05186.0001 

1.02321.0005 

1.02321.0025 

1.02606.0001 

1.02307.0001 

1.02307.0005 

1.03604.0050 

1.04114.0002 

1.13732.0001 

1.05281.0001 

1.05281.0010 

1.05281.0500 

1.07144.0025 

1.07144.1000 

1.07185.0100 

1.07192.0001 

1.16216.0001 

1.07433.0001 

1.07433.0005 

1.07433.0050 

1.12374.0001 

1.08367.1000 

1.08350.0001 

1.08350.0050 

1.08444.0001 

1.24590.0500 

1.08489.0005 

1.08489.0025 

1.16493.0010 

1 vial 

1 vial 

1 g 

25 g 

1 kg 

1 vial 

5 g 

25 g 

1 vial 

1 g 

5 g 

50 g 

2 ml 

1 vial 

1 g 

10 g 

500 g 

25 g 

1 kg 

100 g 

1 g 

1 vial 

1 g 

5 g 

50 g 

1 g 

1 kg 

1 g 

50 g 

5x50 ug 

500 mg 

5 g 

25 g 

10 ml 

Amino Acids 

Buffers 

Benzonase® 





Biochemicals 

Enzymes 

47

ProductFinder 

Ordering information 

In the following catalogue excerpts you will find a comprehensive product 

selection from our main catalogue dedicated to Life Science Products – 

especially for processing purposes. Detailed information concerning 

our products is also published in the Internet under www.merck-lsp.de. 

However, as with all selections, you may just not be able to find the 

product you are looking for in this list. In this case please refer to our 

main catalogue also available as a CD-ROM – or simply to give us a call. 

Our products can also be found in the ChemDAT® listed in the Internet 

under www.merck.de. Even products that are not listed can be supplied. 

One of our strengths is the ability to provide customized solutions. 

Should you require price information in your local currency, please contact 

your local Merck subsidiary. The addresses of the local organisation can be 

found under www.merck.de. 

49

50 

A 

Product Cat.-No. Pack size Page 

ACES 

N-(2-Acetamido)-2-aminoethanesulfonic acid 

N-Acetyl-L-cysteine for biochemistry 

N-Acetyl-DL-tryptophan extra pure low in endotoxins, Ph Eur, BP 

N-Acetyl-DL-tryptophan for biochemistry 

ADA 

N-(2-Acetamido)iminodiacetic acid 

ß-Alanine for biochemistry 

DL-Alanine for biochemistry 

L-Alanine Ph Eur, USP 

L-Alanine for biochemistry 

Albumin fraction V (from bovine serum) for biochemistry 

Aldehyde dehydrogenase (from yeast) lyophilized 100 U/vial 


Aluminium oxide 60, active, basic 

Aluminium oxide 90, active, acidic 

Aluminium oxide 90, active, basic 

Aluminium oxide 90, active, neutral 

Aluminium oxide 90, standardised for chromatographic adsorption analysis 

according to Brockmann 

Aluminium oxide 150, basic 

4-Aminohippuric acid for biochemistry 

5-Aminolevulinic acid hydrochloride for biochemistry 

Ammonium sulfate for biochemistry 

α-Amylase (from porcine pancreas) lyophilized 30,000 U/glass for biochemistry 

β-Amylase (from barley malt) lyophilized 28 U/mg for biochemistry, EC 3.2.1.1 

L-Arginine Ph Eur, USP 

L-Arginine for biochemistry 

L-Arginine monohydrochloride extra pure Ph Eur, BP, USP 

1.15226.0025 

1.15226.0250 

1.15226.9010 

1.12422.0025 

1.12422.0100 

1.12422.1000 

1.12422.9010 

1.12488.1000 

1.12488.5000 

1.12488.9025 

1.12423.0025 

1.15227.0025 

1.15227.9010 

1.01008.0250 

1.01008.1000 

1.00963.0100 

1.00963.1000 

1.01700.0100 

1.01700.1000 

1.01700.9010 

1.01007.0025 

1.01007.0100 

1.01007.1000 

1.01007.9010 

1.12018.0005 

1.12018.0025 

1.12018.0100 

1.12018.0500 

1.15640.0001 

1.01067.1000 

1.01067.2000 

1.01078.1000 

1.01078.2000 

1.01078.9020 

1.01076.1000 

1.01076.2000 

1.01076.9020 

1.01077.1000 

1.01077.2000 

1.01077.9020 

1.01097.1000 

1.01097.1006 

1.01097.5000 

1.01061.1000 

1.01061.2000 

1.00084.0025 

1.00084.0100 

1.24802.0500 

1.24802.2500 

1.01211.0250 

1.01211.1000 

1.01211.5000 

1.01211.9050 

1.16312.0001 

1.01327.0001 

1.01587.0100 

1.01587.1000 

1.01587.9010 

1.01542.0100 

1.01542.1000 

1.01542.9010 

1.01544.0250 

1.01544.1000 

1.01544.5000 

1.01544.9010 

25 g 

250 g 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

1 kg 

5 kg 

25 kg 

25 g 

25 g 

10 kg 

250 g 

1 kg 

100 g 

1 kg 

100 g 

1 kg 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

5 g 

25 g 

100 g 

500 g 

1 vial 

1 kg 

2 kg 

1 kg 

2 kg 

20 kg 

1 kg 

2 kg 

20 kg 

1 kg 

2 kg 

20 kg 

1 kg 

6x1 kg 

5 kg 

1 kg 

2 kg 

25 g 

100 g 

500 mg 

2.5 g 

250 g 

1 kg 

5 kg 

50 kg 

1 vial 

1 g 

100 g 

1 kg 

10 kg 

100 g 

1 kg 

10 kg 

250 g 

1 kg 

5 kg 

10 kg 

15 

12 

12 

12 

15 

12 

12 

12 

12 

43 

47 

33 

33 

33 

33 

33 

33 

12 

12 

43 

47 

47 

12 

12 

12


L-Arginine monohydrochloride for biochemistry 

L-Asparagine monohydrate extra pure DAB 

L-Asparagine monohydrate for biochemistry 

L-Aspartic acid extra pure Ph Eur, BP 

L-Aspartic acid for biochemistry 

L-Aspartic acid Monosodium salt for biochemistry 

Benzonase® ELISA kit II for the immunological detection of Benzonase® 

Benzonase® purity grade I (>99%) for biochemistry 



Benzonase® purity grade II (>90%) for biochemistry 



BES 

N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid 

BICINE 

N,N-Bis(2-hydroxyethyl)-glycine 

BIS-TRIS 

2,2-Bis(hydroxyethyl)-iminotris(hydroxymethyl)-methane 

Brij®35 solution (30% w/w in water) for biochemistry 

Bromelain (from pineapples) 2 mAnson-U/mg for biochemistry, EC 3.4.22.32 

Cacodylic acid sodium salt trihydrate buffer substance 


Catalase (from bovine liver) crystalline suspension in water 15,000,000 U/vial, 

stabilized with 0.01% alkylbenzyldimethylammoniumchloride 


Cellobiose for biochemistry 

Cellulase Onozuka R-10 (from trichoderma viride) 1 U/mg 


Cellulose micro-crystalline Avicel® 

CHAPS (3-[(3-Cholamidopropyl)-dimethylammonio]-propane-sulfonate) 


CHES 

2-(Cyclohexylamino)ethanesulfonic acid 

Cholinesterase (from horse serum) lyophilized 500 U/vial 


Chromolith® Prep Si (100-250mm) 

Chromolith® Prep RP 18 E (100-250mm) 

α-Chymotrypsin (from bovine pancreas) cryst. lyophilized 350 U/mg 


L-Citrulline for biochemistry 

1.01543.0050 

1.01543.0250 

1.01543.1000 

1.01543.9010 

1.01565.0100 

1.01565.1000 

1.01565.5000 

1.01565.9010 

1.01566.0100 

1.01566.1000 

1.01566.9010 

1.00129.0100 

1.00129.1000 

1.00129.5000 

1.00129.9010 

1.00126.0100 

1.00126.1000 

1.00126.9010 

1.00142.0100 

1.00142.1000 

1.01681.0001 

1.01694.0001 

1.01695.0001 

1.01697.0001 

1.01697.0010 

1.01653.0001 

1.01654.0001 

1.01656.0001 

1.15228.0025 

1.15228.0250 

1.15228.9010 

1.01910.0025 

1.01910.0250 

1.01910.9010 

1.03252.0025 

1.03252.0250 

1.01894.0100 

1.01894.1000 

1.01651.0025 

1.01651.1000 

1.03256.0010 

1.03256.0100 

1.05186.0001 

1.02352.0025 

1.02321.0005 

1.02321.0025 

1.02331.0500 

1.02331.2500 

1.02331.9025 

1.11662.0001 

1.11662.0010 

1.15229.0025 

1.15229.0250 

1.02606.001 

1.25251.0001 

1.25252.0001 

1.02307.0001 

1.02307.0005 

1.12117.0050 

1.12117.0250 

50 g 

250 g 

1 kg 

10 kg 

100 g 

1 kg 

5 kg 

10 kg 

100 g 

1 kg 

10 kg 

100 g 

1 kg 

5 kg 

10 kg 

100 g 

1 kg 

10 kg 

100 g 

1 kg 

1 set 

10,000 U 

100,000 U 

500,000 U 

5,000,000 U 

10,000 U 

100,000 U 

500,000 U 

25 g 

250 g 

10 kg 

25 g 

250 g 

10 kg 

25 g 

250 g 

100 ml 

1 l 

25 g 

1 kg 

10 g 

100 g 

1 vial 

25 g 

5 g 

25 g 

500 g 

2.5 kg 

500 g 

1 g 

10 g 

25 g 

250 g 

1 vial 

1 piece 

1 piece 

1 g 

5 g 

50 g 

250 g 

12 

12 

12 

12 

12 

12 

17 

17 

17 

17 

17 

17 

17 

15 

15 

15 

43 

47 

43 

47 

43 

47 

31 

43 

15 

47 

41 

41 

47 

12 

B 

C 

51

52 

D 


Creatinine for biochemistry 

1.05206.0050 

1.05206.0500 

α-Cyclodextrine for biochemistry 

1.02126.0050 

L-Cysteine for biochemistry 

1.02838.0025 

1.02838.0100 

1.02838.1000 

1.02838.9010 

L-Cysteine hydrochloride monohydrate Ph Eur, USP 

1.02735.0100 

1.02735.1000 

1.02735.9010 

L-Cysteine hydrochloride monohydrate for biochemistry 

1.02839.0025 

1.02839.0100 

1.02839.1000 

1.02839.9010 

L-Cystine Ph Eur 

1.02737.0100 

1.02737.1000 

1.02737.9010 

L-Cystine for biochemistry 

1.02837.0025 

1.02837.0100 

1.02837.1000 

1.02837.9010 

Complete column tube (250-50 mm) top flange with screw seal without hydraulics 1.25912.0001 

Complete column tube (155-50 mm) top flange with screw seal without hydraulics 1.25943.0001 

Complete column tube (400 -100 mm) top flange with screw seal without hydraulics 1.25951.0001 

Complete column tube (300 -100 mm) top flange with screw seal without hydraulics 1.25955.0001 

Complete column tube (250-50 mm) with temperature control jacket 

1.25949.0001 

and top flange chain bolted without hydraulics stand 


1.25948.0001 

and top flange with screw seal without hydraulics stand 

Complete column tube (300 -100 mm) with temperature control jacket 

1.25957.0001 



1.25953.0001 

and top flange with screw seal without hydraulics 


1.25954.0001 



1.25950.0001 


Complete column tube (250-50 mm) with top flange chain bolted 

1.25932.0001 


Complete column tube (400 -100 mm) with top flange chain bolted 

1.25952.0001 


Complete column tube (300 -100 mm) with top flange chain bolted 

1.25956.0001 


Complete column tube (260-25 mm) without hydraulics 

1.25941.0001 

Complete column tube (155-50 mm) without hydraulics 

1.25942.0001 

Complete Packing stand NW50 top flange with screw seal, incl. Hydraulics stand 1.25911.0001 

Complete Packing stand NW50 with top flange chain bolted incl. hydraulics stand 1.25931.0001 

Complete Packing stand NW100 top flange with screw seal, incl. Hydraulics stand 1.25930.0001 

Complete Packing stand NW 100 with top flange chain bolted incl. Hydraulics stand 1.25936.0001 

Deoxyribunucleic acid (from herring sperm) sonicated 

for meolecular biology 

Diastase (from fungi) for biochemistry 

Dihydronicotinamide adenine dinucleotide disodium salt 

for biochemistry, NADH-Na2 Dihydronicotinamide adenine dinucleotide phosphate tetrasodium salt for 

biochemistry, NADPH-Na4 5-Dimethylaminonaphthalene-1-sulfonyl chloride (Dansylchloride) 


3-(4,5-Dimethyl-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide 


1.24711.0010 

1.03604.0050 

1.24644.0001 

1.24644.0005 

1.24540.0050 

1.24540.0250 

1.03094.0001 

1.03094.0010 

1.11714.0001 

1.11714.0010 

50 g 

500 g 

50 g 

25 g 

100 g 

1 kg 

10 kg 

100 g 

1 kg 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

100 g 

1 kg 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

1 

10 mg 

50 g 

1 g 

5 g 

50 mg 

250 mg 

1 g 

10 g 

1 g 

10 g 

43 

43 

12 

12 

12 

12 

12 

38 

38 

38 

38 

38 

38 

38 

38 

38 

37 

38 

38 

38 

37 

37 

38 

38 

38 

38 

43 

47 

43 

43 

43 

43


2,2-Dinitro-5,5-dithiodibenzoic acid for biochemistry 

1,4-Dithioerythritol for biochemistry 

1,4-Dithiothreitol for biochemistry 

Ethylene glycol monomethyl ether for amino acid analysis 

Filter for Chromolith® Prep Si 

Fitting Set for Packing stand NW25 and NW50 

Florisil® 

Florisil® for residual analysis 

Folic acid for biochemistry 

Fractogel® EMD Amino (M) 

Fractogel® EMD BioSEC (S) 

Fractogel® EMD Chelate (M) 

Fractogel® EMD COO – (M) 

Fractogel® EMD COO – (S) 

Fractogel® EMD DEAE (M) 

Fractogel® EMD DEAE (S) 

Fractogel® EMD DMAE (M) 

Fractogel® EMD DMAE (S) 

Fractogel® EMD Phenyl (S) 

Fractogel® EMD Propyl (S) 

Fractogel® EMD SE Hicap (M) 

Fractogel® EMD SO 3 – (M) 

Fractogel® EMD SO 3 – (S) 

Fractogel® EMD TA (S) 

Fractogel® EMD TMAE (M) 

Fractogel® EMD TMAE (S) 

1.03291.0001 

1.03291.0005 

1.03291.0025 

1.24511.0001 

1.24511.0005 

1.24511.0025 

1.11474.0001 

1.11474.0005 

1.11474.0025 

1.15118.1000 

1.15118.2500 

1.15118.9026 

1.25253.0001 

1.25005.0001 

1.12518.0100 

1.12518.1000 

1.12994.0100 

1.12994.1000 

1.03984.0005 

1.03984.0025 

1.14893.0500 

1.14893.5000 

1.10317.0250 

1.10317.1000 

1.10317.5000 

1.10338.0250 

1.10338.0500 

1.10338.5000 

1.16886.0100 

1.16886.0500 

1.16886.5000 

1.16891.0100 

1.16891.0500 

1.16883.0100 

1.16883.0500 

1.16883.5000 

1.16888.0100 

1.16888.0500 

1.16884.0100 

1.16884.0500 

1.16884.5000 

1.16889.0100 

1.16889.0500 

1.16197.0100 

1.16197.0500 

1.10085.0100 

1.10085.0500 

1.14894.0100 

1.14894.0500 

1.14894.5000 

1.16882.0100 

1.16882.0500 

1.16882.5000 

1.16890.0100 

1.16890.0500 

1.16473.0025 

1.16473.0250 

1.16473.1000 

1.16881.0100 

1.16881.0500 

1.16881.5000 

1.16887.0100 

1.16887.0500 

1 g 

5 g 

25 g 

1 g 

5 g 

25 g 

1 g 

5 g 

25 g 

1 l 

2.5 l 

25 l 

10 pieces 

1 

100 g 

1 kg 

100 g 

1 kg 

5 g 

25 g 

500 ml 

5000 ml 

250 ml 

1000 ml 

5000 ml 

250 ml 

500 ml 

5000 ml 

100 ml 

500 ml 

5000 ml 

100 ml 

500 ml 

100 ml 

500 ml 

5000 ml 

100 ml 

500 ml 

100 ml 

500 ml 

5000 ml 

100 ml 

500 ml 

100 ml 

500 ml 

100 ml 

500 ml 

100 ml 

500 ml 

5000 ml 

100 ml 

500 ml 

5000 ml 

100 ml 

500 ml 

25 ml 

250 ml 

1000 ml 

100 ml 

500 ml 

5000 ml 

100 ml 

500 ml 

43 

43 

43 

43 

41 

37, 38 

31 

31 

23 

25 

23 

21 

21 

21 

21 

21 

21 

23 

23 

21 

21 

21 

23 

21 

21 

E 

F 

53

54 

G 


Fractogel® EMD TMAE Hicap (M) 


Fractoprep® SO 3 

Fractoprep® TMAE 

Frit holder for compression piston for Packing stand NW25 

Frit holder for compression piston NW50 

Frit holder for compression piston NW100 

D(-)-Fructose for biochemistry 

D(+)-Galactose for biochemistry 

Gasket Set (outlet cover plate) for NW25 

Gasket Set (outlet cover plate) for NW50 

Gasket Set (upper outlet cover plate) for NW100 

β-Glucoronidase/Arylsulfatase (from helix pomatia) stabilized 

aqueous solution for biochemistry, EC 3.2.1.31 + EC 3.1.6.1 

D(+)-Glucose anhydrous for biochemistry 

D(+)-Glucose monohydrate for biochemistry 

Glucose dehydrogenase (from bacillus megaterium recombinant) 

lyophilized 250 U/vial for biochemistry, EC 1.1.1.47 

L-Glutamic acid Ph Eur 

L-Glutamic acid for biochemistry 

L-Glutamine DAB, USP 

L-Glutamine for biochemistry 

Glutathione (reduced) for biochemistry 

Glycerol triacetate for biochemistry 

Glycerol triacetate Ph Eur, BP, USP 

Glycine cryst. Ph Eur, BP, USP 

Glycine GR for analysis 

Glycylglycine 

1.10316.0100 

1.10316.0500 

1.10316.5000 

1.17971.0100 

1.17971.1000 

1.17971.5000 

1.17972.0100 

1.17972.1000 

1.17972.5000 

1.17973.0100 

1.17973.1000 

1.17973.5000 

1.25010.0001 

1.25908.0001 

1.25934.0001 

1.04007.1000 

1.04058.0025 

1.04058.0100 

1.25014. 0001 

1.25913.0001 

1.25937.0001 

1.04114.0002 

1.08337.0250 

1.08337.1000 

1.08337.5000 

1.08337.9050 

1.04074.1000 

1.04074.5000 

1.04074.9050 

1.13732.0001 

1.01791.0100 

1.01791.1000 

1.01791.9010 

1.00291.0100 

1.00291.0250 

1.00291.1000 

1.00291.9010 

1.00286.0100 

1.00286.1000 

1.00286.9010 

1.00289.0025 

1.00289.0100 

1.00289.1000 

1.00289.9010 

1.04090.0001 

1.04090.0005 

1.04090.0050 

1.04090.0500 

1.08238.0500 

1.03000.1000 

1.03000.9010 

5.00190.1000 

5.00190.5000 

5.00190.9025 

1.04201.0100 

1.04201.0250 

1.04201.1000 

1.04201.5000 

1.04201.9025 

1.04233.0010 

1.04233.0100 

1.04233.1000 

1.04233.9010 

100 ml 

500 ml 

5000 ml 

100 ml 

1000 ml 

5000 ml 

100 ml 

1000 ml 

5000 ml 

100 ml 

1000 ml 

5000 ml 

1 

1 

1 

1 kg 

25 g 

100 g 

1 

1 

1 

2 ml 

250 g 

1 kg 

5 kg 

50 kg 

1 kg 

5 kg 

50 kg 

1 vial 

100 g 

1 kg 

10 kg 

100 g 

250 g 

1 kg 

10 kg 

100 g 

1 kg 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

1 g 

5 g 

50 g 

500 g 

500 ml 

1 l 

10 l 

1 kg 

5 kg 

25 kg 

100 g 

250 g 

1 kg 

5 kg 

25 kg 

10 g 

100 g 

1 kg 

10 kg 

21 

21 

21 

21 

37 

38 

38 

43 

43 

37 

38 

38 

47 

43 

43 

47 

13 

13 

13 

13 

44 

44 

44 

13 

13 

15


Guanidinium chloride for biochemistry 

Guanidinium chloride LAB 

Guanidinium thiocyanate for biochemistry 

HEPES 


HEPES for biotechnology 


HEPES Sodium 

2-[4-(2-Hydroxyethyl-1-piperazine)]ethanesulfonic acid monosodium salt 

HEPPS 

3-[4-(2-Hydroxyethyl-1-piperazinyl)]-propanesulfonic acid 

Hibar® column LiChrosorb® RP-select B 

Hibar® column LiChrosorb® RP-18 

Hibar® column LiChrosorb® RP-8 

Hibar® column LiChrosorb® Si 60 

Hibar® column LiChrospher® 100 RP-18 

Hibar® column LiChrospher® 100 RP-8 

Hibar® column LiChrospher® 60 RP-select B 

Hibar® column LiChrospher® Si 60 

Hippuric acid LAB 

L-Histidine Ph Eur, USP 

L-Histidine for biochemistry 

L-Histidine monohydrochloride extra pure Ph Eur,BP 

L-Histidine monohydrochloride for biochemistry 

Hydraulics stand for column tube NW25 complete plus filling tube 

Hydraulics stand complete (for column tube NW 50 top flange 

with screw seal plus filling tube) 

Hydraulics stand complete (for column tube NW 50 with top 

flange chain bolted plus filling tube) 

Hydraulics stand complete (for column tube NW100 top flange 

with screw seal plus filling tube and basic stand) 

Hydraulics stand complete (for column tube NW 100 with top flange 

chain bolted plus filling tube and basic stand) 

L-Hydroxyproline for biochemistry 

Imidazol 

1.04219.0025 

1.04219.0100 

1.04219.0500 

1.04220.0100 

1.04220.1000 

1.04220.5000 

1.04220.9010 

1.04220.9025 

1.04167.0025 

1.04167.0250 

1.10110.0025 

1.10110.0250 

1.10110.1000 

1.10110.9010 

1.10110.9025 

1.17839.0100 

1.17839.1000 

1.17839.9010 

1.15231.0025 

1.15231.0250 

1.15231.2500 

1.15231.9010 

1.15230.0025 

1.15230.0250 

1.15230.2500 

1.15230.9010 

1.51464.0001 

1.51494.0001 

1.51441.0001 

1.51435.0001 

1.51483.0001 

1.51482.0001 

1.51484.0001 

1.51485.0001 

1.00296.0500 

1.00296.2500 

1.00296.9025 

1.04352.0100 

1.04352.1000 

1.04352.9010 

1.04351.0025 

1.04351.0100 

1.04351.1000 

1.04351.9010 

1.04354.0500 

1.04354.5000 

1.04350.0025 

1.04350.0100 

1.04350.0500 

1.04350.9010 

1.25946.0001 

1.25945.0001 

1.25947.0001 

1.25958.0001 

1.25959.0001 

1.04510.0010 

1.04510.0100 

1.04716.0050 

1.04716.0250 

1.04716.1000 

1.04716.9025 

25 g 

100 g 

500 g 

100 g 

1 kg 

5 kg 

10 kg 

25 kg 

25 g 

250 g 

25 g 

250 g 

1 kg 

10 kg 

25 kg 

100 g 

1 kg 

10 kg 

25 g 

250 g 

2.5 kg 

10 kg 

25 g 

250 g 

2.5 kg 

10 kg 

1 piece 

1 piece 

1 piece 

1 piece 

1 piece 

1 piece 

1 piece 

1 piece 

500 g 

2.5 kg 

25 kg 

100 g 

1 kg 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

500 g 

5 kg 

25 g 

100 g 

500 g 

10 kg 

1 

1 

1 

1 

1 

10 g 

100 g 

50 g 

250 g 

1 kg 

25 kg 

44 

44 

44 

15 

15 

15 

15 

35 

35 

35 

35 

35 

35 

35 

35 

13 

13 

13 

13 

13 

37 

38 

38 

38 

38 

13 

15 

H 

I, J, K 

55

56 

L 


Indole-3-acetic acid for biochemistry 

Indole-3-butyric acid for biochemistry 

myo-Inositol for biochemistry 

L-Isoleucine Ph Eur, USP 

L-Isoleucine for biochemistry 

Lactose monohydrate for biochemistry 

L-Leucine Ph Eur, USP 

L-Leucine for biochemistry 

LiChroprep® CN 

LiChroprep® DIOL 

LiChroprep® NH2 LiChroprep® NH2 LiChroprep® RP-18 





LiChroprep® RP-select B 












LiChrospher® WP 300 RP-18 

Lithiumdodecylsulfat for biochemistry 

1.00353.0010 

1.00353.0100 

1.00353.1000 

1.00354.0005 

1.00354.0025 

1.00354.0100 

1.00354.0500 

1.04507.0050 

1.04507.0250 

1.05357.0100 

1.05357.1000 

1.05357.9010 

1.05362.0025 

1.05362.0100 

1.05362.0500 

1.05362.9010 

1.07660.0250 

1.07660.1000 

1.07660.9010 

1.05350.0100 

1.05350.1000 

1.05350.9010 

1.05360.0025 

1.05360.0250 

1.05360.9010 

1.13959.0250 

1.13973.0250 

1.13925.0100 

1.13974.0250 

1.13902.0100 

1.13901.0500 

1.09303.0100 

1.09303.0500 

1.09303.9025 

1.13900.0250 

1.13900.1000 

1.09304.0100 

1.09302.0100 

1.09302.1000 

1.13904.1000 

1.09319.0100 

1.09336.1000 

1.09336.9025 

1.09390.1000 

1.13905.0250 

1.13905.1000 

1.19656.0100 

1.19656.0500 

1.11022.0100 

1.11022.0500 

1.11022.9025 

1.19655.0100 

1.19655.0500 

1.11023.0100 

1.11023.0500 

1.19654.0100 

1.19654.1000 

1.11024.0100 

1.11024.1000 

1.11024.9025 

1.19659.0100 

1.19659.0500 

1.14243.0010 

1.14243.0050 

10 g 

100 g 

1 kg 

5 g 

25 g 

100 g 

500 g 

50 g 

250 g 

100 g 

1 kg 

10 kg 

25 g 

100 g 

500 g 

10 kg 

250 g 

1 kg 

10 kg 

100 g 

1 kg 

10 kg 

25 g 

250 g 

10 kg 

250 g 

250 g 

100 g 

250 g 

100 g 

500 g 

100 g 

500 g 

25 kg 

250 g 

1 kg 

100 g 

100 g 

1 kg 

1 kg 

100 g 

1 kg 

25 kg 

1 kg 

250 g 

1 kg 

100 g 

500 g 

100 g 

500 g 

25 kg 

100 g 

500 g 

100 g 

500 g 

100 g 

1 kg 

100 g 

1 kg 

25 kg 

100 g 

500 g 

10 g 

50 g 

44 

44 

44 

13 

13 

44 

13 

13 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

29 

44


Lithium hydroxide monohydrate for amino acid analysis 

Lobar® assembly kit 

Lobar® connectors (dead-volume free) 

Lobar® glass columns basic equipment 

Lobar® column LiChroprep® RP-18 



Lobar® column LiChroprep® Si 60 



Lobar® nuts 

Lobar® PTFE gripper with stainless steel retainer 

L-Lysine monohydrate for biochemistry 

L-Lysine monohydrochloride extra pure Ph Eur, BP, USP 

L-Lysine monohydrochloride for biochemistry 

Lysozyme (from egg white) 50,000 U/mg cryst. 

(hydrochloride) for biochemistry, EC 3.2.1.17 

DL-Malic acid for biochemistry 

L(-)-Malic acid for biochemistry 

Maltose monohydrate for biochemistry 

D(+)-Mannose for biochemistry 

Melibiose monohydrate for biochemistry 

MES 

2-Morpholinoethanesulfonic acid monohydrate 

L-Methionine for biochemistry 

MOPS 

3-Morpholinopropanesulfonic acid 

1-Naphthylacetic acid for biochemistry 

Nicotinamide adenine dinucleotide free acid for biochemistry, 

NAD 

Nicotinamide adenine dinucleotide phosphate disodium salt 

for biochemistry, NADP-Na2 

L-Norleucine for biochemistry 

Octanoic acid for biochemistry 

O-ring for new frit holder NW100 

L-Ornithine monohydrochloride for biochemistry 

1.11652.0250 

1.11652.1000 

1.15395.0001 

1.15455.0001 

1.10407.0001 

1.10624.0001 

1.10625.0001 

1.10626.0001 

1.10400.0001 

1.10401.0001 

1.10402.0001 

1.15393.0001 

1.15396.0001 

1.12233.0100 

1.12233.1000 

1.05701.1000 

1.05701.5000 

1.05701.9025 

1.05700.0100 

1.05700.1000 

1.05700.9010 

1.05281.0001 

1.05281.0010 

1.05281.0500 

1.00382.0250 

1.00382.1000 

1.00381.0005 

1.00381.0025 

1.00381.0100 

1.05912.0025 

1.05912.0100 

1.05388.0025 

1.05388.0100 

1.12240.0005 

1.12240.0025 

1.06126.0025 

1.06126.0250 

1.06126.9010 

1.05707.0025 

1.05707.0100 

1.05707.1000 

1.05707.9010 

1.06129.0025 

1.06129.0250 

1.06129.9010 

1.06220.0010 

1.06220.0100 

1.24542.0001 

1.24542.0005 

1.24542.0025 

1.24541.0001 

1.24560.0250 

1.00193.0250 

1.00193.2500 

1.00193.9023 

1.25960.0001 

1.06906.0025 

1.06906.0100 

1.06906.1000 

1.06906.9010 

250 g 

1 kg 

1 

1 

1 

1 piece 

1 piece 

1 piece 

1 piece 

1 piece 

1 piece 

1 

1 

100 g 

1 kg 

1 kg 

5 kg 

25 kg 

100 g 

1 kg 

10 kg 

1 g 

10 g 

500 g 

250 g 

1 kg 

5 g 

25 g 

100 g 

25 g 

100 g 

25 g 

100 g 

5 g 

25 g 

25 g 

250 g 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

25 g 

250 g 

10 kg 

10 g 

100 g 

1 g 

5 g 

25 g 

1 g 

250 mg 

250 ml 

2.5 l 

23 l 

5 

25 g 

100 g 

1 kg 

10 kg 

44 

35 

35 

35 

35 

35 

35 

35 

35 

35 

35 

35 

13 

13 

13 

47 

44 

44 

44 

44 

44 

15 

13 

15 

44 

44 

44 

13 

44 

38 

13 

M 

N 

O 

57

58 

P 

Q,R 

S 


2-Oxoglutaric acid for biochemistry 

Packing stand NW25 complete with hydraulics stand 

Papain (from carica papaya) water-soluble 30,000 USP-U/mg 


PEEK Connecting Set for Packing stand NW25 and NW50 

PefablocR SC (4-(2-Aminoethyl)-benzolsulfonylfluoridhydrochloride) 

(AEBSF) for biochemistry 

Pepsin (from porcine gastric mucosa) 0.7 FIP-U/mg 


Pepsin (from porcine gastric mucosa) cryst. lyophilized 

10 FIP-U/mg for biochemistry, EC 3.4.23.1 

Peroxidase (from horse radish) 10,000 U/vial for biochemistry, EC 1.11.1.7 




DL-Phenylalanine for biochemistry 

L-Phenylalanine Ph Eur, USP 

L-Phenylalanine for biochemistry 

Phthaldialdehyde for fluorometry 

PIPES 

Piperazine-1,4-bis(2-ethanesulfonic acid) 

PIPES Sodium 

Piperazine-1,4-bis(2-ethanesulfonic acid) monosodium salt 

L-Proline Ph Eur, USP 

L-Proline for biochemistry 

Pronase E (from streptomyces griseus) 4,000,000 PU/g 


Protamine sulfate for biochemistry 

Pyruvic acid sodium salt for biochemistry 

Raffinose pentahydrate for biochemistry 

Replacement frits for Hibar® columns 

L(+)-Rhamnose monohydrate for biochemistry 

D(-)-Ribose for biochemistry 

Sealing ring for column NW25 

Sealing ring for column NW50 

1.05194.0025 

1.05194.0100 

1.05194.1000 

1.25940.0001 

1.07144.0025 

1.07144.1000 

1.25914.0001 

1.24839.0100 

1.24839.0500 

1.07185.0100 

1.07192.0001 

1.16216.0001 

1.09371.0100 

1.09371.1000 

1.09371.5000 

1.09372.0100 

1.09372.1000 

1.09372.5000 

1.09373.0100 

1.09373.1000 

1.09373.5000 

1.07257.0025 

1.07257.0100 

1.07267.0100 

1.07267.1000 

1.07267.9010 

1.07256.0025 

1.07256.0100 

1.07256.1000 

1.07256.9010 

1.11452.0005 

1.11452.0050 

1.10220.0025 

1.10220.0250 

1.10220.1000 

1.10220.9010 

1.15232.0025 

1.15232.0250 

1.07430.0100 

1.07430.1000 

1.07430.9010 

1.07434.0010 

1.07434.0100 

1.07434.0500 

1.07434.9010 

1.07433.0001 

1.07433.0005 

1.07433.0050 

1.10123.0005 

1.10123.0025 

1.06619.0050 

1.06619.0250 

1.06619.1000 

1.07419.0050 

1.51221.0001 

1.07361.0010 

1.07361.0025 

1.07605.0050 

1.25013.0001 

1.25909.0001 

25 g 

100 g 

1 kg 

1 

25 g 

1 kg 

1 

100 mg 

500 mg 

100 g 

1 g 

1 vial 

100 g 

1 kg 

5 kg 

100 g 

1 kg 

5 kg 

100 g 

1 kg 

5 kg 

25 g 

100 g 

100 g 

1 kg 

10 kg 

25 g 

100 g 

1 kg 

10 kg 

5 g 

50 g 

25 g 

250 g 

1 kg 

10 kg 

25 g 

250 g 

100 g 

1 kg 

10 kg 

10 g 

100 g 

500 g 

10 kg 

1 g 

5 g 

50 g 

5 g 

25 g 

50 g 

250 g 

1 kg 

50 g 

1 piece 

10 g 

25 g 

50 g 

1 

2 

44 

37 

47 

37, 38 

44 

47 

47 

47 

33 

33 

33 

13 

13 

13 

44 

15 

15 

13 

13 

47 

44 

44 

44 

35 

45 

45 

37 

38


Sealing ring for piston NW 100 

L-Serine Ph Eur, USP 

L-Serine for biochemistry 

Silica gel 40 

Silica gel 40 

Silica gel 60 

Silica gel 60 

Silica gel 60 

Silica gel 60 

Silica gel 60 

Silica gel 60 

Silica gel 60 extra pure 

Silica gel 60 F254 

Silica gel 60 RP-18 

Silica gel 60 silanised (dimethylsilane derivate) 



tri-Sodium citrate dihydrate buffer substance 


D(-)-Sorbitol 

Special packing for Hibar® packed column (250 - 25 mm) 

Special packing for Hibar® pre-packed column (250 - 25 mm) 

Special packing for Hibar® pre-packed column (125 - 25 mm) 

Starch (from wheat) for biochemistry 

Starch soluble GR for analysis, ISO 

Starch soluble acc. to Zulkowsky GR for analysis 

Sucrose (saccharose) for biochemistry 

Sucrose (saccharose) for density gradient ultracentrifugation 

1.25935.0001 

1.07647.0100 

1.07647.1000 

1.07647.9010 

1.07769.0010 

1.07769.0100 

1.07769.1000 

1.07769.9010 

1.10180.1000 

1.10180.5000 

1.10181.1000 

1.10181.9025 

1.07729.1000 

1.07729.5000 

1.07729.9025 

1.07733.0500 

1.07733.1000 

1.07733.5000 

1.07733.9025 

1.07734.1000 

1.07734.2500 

1.07734.5000 

1.07734.9025 

1.09385.1000 

1.09385.2500 

1.09385.5000 

1.09385.9025 

1.15101.1000 

1.15101.9025 

1.15111.1000 

1.15111.2500 

1.15111.9025 

1.07754.0500 

1.07754.1000 

1.10757.1000 

1.10167.1000 

1.10167.5000 

1.10167.9025 

1.07719.0250 

1.07719.1000 

1.10184.0500 

1.10184.5000 

1.10185.0500 

1.10185.9025 

1.06430.1000 

1.07759.0100 

1.07759.0500 

1.07759.5000 

1.50099.0001 

1.50004.0001 

1.50016.0001 

1.11685.0250 

1.11685.1000 

1.11685.9025 

1.01252.0100 

1.01252.0250 

1.01252.1000 

1.01252.9025 

1.01257.0050 

1.01257.0250 

1.01257.1000 

1.01257.9025 

1.07687.0250 

1.07687.1000 

1.07687.5000 

1.07654.1000 

1.07654.5000 

2 

100 g 

1 kg 

10 kg 

10 g 

100 g 

1 kg 

10 kg 

1 kg 

5 kg 

1 kg 

25 kg 

1 kg 

5 kg 

25 kg 

500 g 

1 kg 

5 kg 

25 kg 

1 kg 

2.5 kg 

5 kg 

25 kg 

1 kg 

2.5 kg 

5 kg 

25 kg 

1 kg 

25 kg 

1 kg 

2.5 kg 

25 kg 

500 g 

1 kg 

1 kg 

1 kg 

5 kg 

25 kg 

250 g 

1 kg 

500 g 

5 kg 

500 g 

25 kg 

1 kg 

100 g 

500 g 

5 kg 

1 piece 

1 piece 

1 piece 

250 g 

1 kg 

25 kg 

100 g 

250 g 

1 kg 

25 kg 

50 g 

250 g 

1 kg 

25 kg 

250 g 

1 kg 

5 kg 

1 kg 

5 kg 

38 

13 

13 

27 

27 

27 

27 

27 

27 

27 

27 

27 

27 

31 

31 

27 

27 

45 

45 

37 

37 

37 

45 

45 

45 

45 

45 

59

60 

T 


Teflon lined flexible stainless steel tube (1.5m x 1/4") Set 

TES 

N-[Tris(hydroxymethyl)-methyl]-2-aminoethanesulfonic acid 

Thiamine chloride hydrochloride for biochemistry 

L-Threonine for biochemistry 

Thrombin (from bovine plasma) 50 NIH-U/mg for biochemistry, 

EC 3.4.21.5 

Titanium(III) chloride solution about 15% (in about 10% hydrochloric acid) 


Trehalose for biochemistry 

TRICINE 

N-[Tris(hydroxymethyl)-methyl]-glycine 

Triethanolamine Ph Eur 

Triethanolamine GR for analysis 

Triethanolamine hydrochloride GR for analysis 

Tripod for Packing stand NW25 and NW50 

TRIS Ph Eur, BP, USP 


TRIS GR for analysis, ACS 


TRIS LAB 


TRIS-hydrochloride GR for analysis 

Tris(hydroxymethyl)aminomethane hydrochloride 

Trypsin (from porcine pancreas) cryst. lyophilized 40 U/mg 


Trypsin (from porcine pancreas) lyophilized, purified by affinity 

chromatography for protein sequence analysis, EC 3.4.21.4 

Trypsin (from porcine pancreas), TPCK-treated lyophilized 40 U/mg 


Trypsin (pancreas protease), 200 FIP-U/g, EC 3.4.21.4 

DL-Tryptophane for biochemistry 

L-Tryptophane extra pure Ph Eur, BP, USP 

L-Tryptophane for biochemistry 

L-Tyrosine Ph Eur, USP 

1.25938.0001 

1.10695.0025 

1.10695.0250 

1.10695.9010 

1.08181.0025 

1.08181.0250 

1.08411.0010 

1.08411.0100 

1.08411.1000 

1.08411.9010 

1.12374.0001 

1.10789.0001 

1.08216.0010 

1.08216.0025 

1.08216.0100 

1.08216.5000 

1.08602.0025 

1.08602.0250 

1.08602.9010 

1.08372.1000 

1.08372.5000 

1.08372.9025 

1.08379.0250 

1.08379.1000 

1.08379.9026 

1.08357.0100 

1.08357.1000 

1.08357.9025 

1.25961.0001 

1.08386.1000 

1.08386.5000 

1.08386.9025 

1.08382.0100 

1.08382.0500 

1.08382.1000 

1.08382.2500 

1.08382.5000 

1.08382.9025 

1.08387.0500 

1.08387.2500 

1.08219.0100 

1.08219.1000 

1.08219.9010 

1.08219.9025 

1.08350.0001 

1.08350.0050 

1.08444.0001 

1.24590.0500 

1.08367.1000 

1.08375.0025 

1.08375.0100 

1.08396.0100 

1.08396.1000 

1.08396.5000 

1.08374.0010 

1.08374.0100 

1.08374.0500 

1.08374.9010 

1.08378.0100 

1.08378.1000 

1.08378.9010 

1 

25 g 

250 g 

10 kg 

25 g 

250 g 

10 g 

100 g 

1 kg 

10 kg 

1 g 

6x7.5 ml 

10 g 

25 g 

100 g 

5 kg 

25 g 

250 g 

10 kg 

1 l 

5 l 

25 l 

250 ml 

1 l 

25 l 

100 g 

1 kg 

25 kg 

1 

1 kg 

5 kg 

25 kg 

100 g 

500 g 

1 kg 

2.5 kg 

5 kg 

25 kg 

500 g 

2.5 kg 

100 g 

1 kg 

10 kg 

25 kg 

1 g 

50 g 

5x50 ug 

500 mg 

1 kg 

25 g 

100 g 

100 g 

1 kg 

5 kg 

10 g 

100 g 

500 g 

10 kg 

100 g 

1 kg 

10 kg 

38 

15 

13 

47 

45 

45 

15 

45 

45 

45 

37, 38 

15 

15 

15 

15 

47 

47 

47 

47 

13 

13 

13 

13


L-Tyrosine for biochemistry 

Urea for biochemistry 

Urease (from jack beans) lyophilized 5 U/mg for the 

determination of urea in serum, EC 3.5.1.5 

Urease (from jack beans) solution in 50% glycerol, 1000 U/ml for biochemistry, 

EC 3.5.1.5 

Uric acid for biochemistry 

L-Valine for biochemistry 

Vitamin B12 for biochemistry 

D(+)-Xylose for biochemistry 

1.08371.0025 

1.08371.0100 

1.08371.1000 

1.08371.9010 

1.08488.0250 

1.08488.1000 

1.08488.5000 

1.08488.9010 

1.08488.9025 

1.08489.0005 

1.08489.0025 

1.16493.0010 

1.00817.0025 

1.00817.0100 

1.08495.0025 

1.08495.0100 

1.08495.1000 

1.08495.9010 

1.24592.0100 

1.24592.1000 

1.08689.0025 

1.08689.0100 

25 g 

100 g 

1 kg 

10 kg 

250 g 

1 kg 

5 kg 

10 kg 

25 kg 

5 g 

25 g 

10 ml 

25 g 

100 g 

25 g 

100 g 

1 kg 

10 kg 

100 mg 

1 kg 

25 g 

100 g 

13 

45 

47 

47 

47 

13 

45 

45 

U 

V 

W,X,Y,Z 

61

62 Appendix 

Appendix 

Abbreviations 

ACS American Chemical Society 

AFNOR Association Française de Normalisation 

ASTM American Society for Testing and Materials 

BP British Pharmacopoeia 

CAS Chemical Abstracts Service 

Cod Franç Codex Français 

DAB Deutsches Arzneibuch 

DAC Deutscher Arzneimittel-Codex 

DEAE Diethylaminoethyl 

DIN Deutsches Institut für Normung 

DMAE Dimethylaminoethyl 

DNA Deoxyribonucleic acid 

EC Enzyme Commission 

ELISA Enzyme Linked Immuno Sorbent Assay 

FIP International Pharmaceutical Federation 

(Fédération Internationale Pharmaceutique) 

GC Gas chromatography 

GMP Good Manufacturing Practise 

HIC Hydrophobic Interaction Chromatography 

HPLC High Performance Liquid Chromatography 

IEX Ion Exchange Chromatography 

IR Infrared spectroscopy 

ISO International Organisation for Standardisation 

IUPAC International Union of Pure and Applied Chemistry 

JP The Pharmacopoeia of Japan 

NBS National Bureau of Standards 

NIST National Institute of Standards and Technology (since 1989) 

NF The National Formulary (USA) 

PE Polyethylene 

Ph Eur European Pharmacopöe 

Ph Helv Pharmacopoea Helvetica 

Ph Nord Pharmacopoea Nordica 

PTFE Polytetrafluoroethylen 

PVDV Polyvinylidene difluoride 

RNA Ribonucleic acid 

RP Reversed Phase 

RSF Regulatory Support File 

RTECS Registry of Toxic Effects of Chemical Substances 

SE Sulfoethyl- 

SEC Size Exclusion Chromatography 

(Gel permeation chromatography) 

SMB Simulated Moving Bed 

TA Thiophilic Adsorption 

TLC Thin layer chromatography 

TMAE Trimethylaminoethyl 

USP The United States Pharmacopoeia 

Hazard Symbols and their Meanings 

Symbols 

Type of hazard 

E 

O 

T+ 

T 

Xn 

F+ 

F 

C 

Xi 

N 

Explosive 

Oxidizing 

Very toxic 

Toxic 

Harmful 

Extremely flammable 

Highly flammable 

Corrosive 

Irritant 

Dangerous for the environment

Safety in the Laboratory 

Are you in charge of a laboratory? 

If so, you are also responsible for the safety and 

well-being of all persons working there as well as 

for the environment. 

Your duties include being aware of what might 

happen, establishing what must not happen, and 

implementing measures aimed at avoiding 

accidents. 

· When planning an experiment, decide whether 

hazardous substances are in fact required or 

whether a less hazardous substance might not be 

used instead. (To help you, we have published 

information in a Chemical Data Base under 

www.chemdat.de). 

· In addition to the risks that the substances 

themselves pose, you must establish if there are 

any risks specific to your factory or plant. Find out 

whether any hazardous chemical reactions could 

conceivably occur and decide how these can be 

avoided. 

· Specify all measures needed in order to assure 

personal health and safety, according to the 

ranking technical – organizational – personal, and 

check these are in place before any hazardous 

substances are handled. 

· Ensure that hazardous substances are shelved and 

stored in such a way that they can pose no threat 

to human health and the environment. Restrict the 

amounts of combustible substances used in the 

laboratory to the absolute minimum needed. 

· Issue workers with suitable, effective personal 

protective clothing and equipment. Ensure that it 

is kept clean and in good condition. 

· Compose a set of laboratory rules that cover all of 

the potential health and environmental risks that 

may be encountered in the laboratory, and also 

specify general preventive measures and rules of 

conduct. 

· Compile specific operating procedures for activities 

that involve the use of hazardous substances (see 

SafeDAT software (item no. 403032)). Display these 

operating procedures prominently within the laboratory, 

and use them to instruct workers at least 

once a year in the handling of hazardous substances 

and in the potential dangers of working with 

them. 

· Always strive to improve industrial safety and 

personal well-being in the area for which you are 

responsible. 

To find out more on how you should handle our 

reagents, please speak to your contact person 

within your local Merck dealership. This person 

can then, if necessary, contact our Industrial 

Safety Department for further information. 

Do you work in a laboratory? 

When handling chemicals, always follow safety 

procedures, even if the chemical in question is not 

labeled as hazardous. 

· Make sure the laboratory is kept clean and tidy. 

· Use only such hazardous substances and 

equipment as are absolutely necessary for you to 

carry out your duties. 

· Always ensure that the sash front is down on any 

fume hood. 

· If possible, keep hazardous substances in their 

original Merck containers and packaging. These are 

able to withstand normal conditions of use and are 

suitably labeled. 

· If you need to use a different container for day-today 

use, ensure it is at least labeled with the name 

of the chemical, its hazard symbol(s) and clear text 

warnings. Keep containers with hazardous substances 

no higher than the height at which they 

can still be safely dispensed and shelved. 

63

64 Appendix 

· Where possible, keep very toxic and toxic 

substances under lock and key. 

·Keep any substance that emits harmful vapors or 

gases in a place that allows these to be permanently 

extracted. 

· Check the laboratory inventory at least annually 

and dispose of any chemicals that have become 

unusable or no longer required. 

· Never pipet by mouth. Always use a mechanically 

operated pipet. 

· Do not eat, drink or smoke in the labo-ratory. 

· Never keep food or drink in the vicinity of 

chemicals. Never place chemicals in containers 

that are normally used for food or drink. 

· In the event of a potentially harmful leakage of 

gases, vapors or particles leave the laboratory 

immediately and warn persons nearby. Before any 

attempt is made to eliminate the danger, take 

appropriate measures. 

· Always work under a hood if you are using 

spontaneously combustible substances, and keep a 

suitable fire extinguisher close to hand. 

· Use Perex-Test® (item no. 116206) to test for 

peroxides in liquids that show a tendency to form 

them, especially before distilling or evaporating 

the liquid. Dispose of them properly. 

·Keep liquids that show a tendency to form organic 

peroxides away from light. This is no guarantee, 

however, that peroxides will not form. 

· Always use the personal protective clothing and 

equipment that was issued to you. However, the 

use of respirators and full-body protection should 

be the exception rather than the rule. 

·Wear suitable clothing and sturdy, closed, non-slip 

footware when working in the laboratory. 

· Always wear safety spectacles affording adequate 

protection at the top and sides when working in 

the laboratory. 

· Always wear protective gloves when the hands in 

particular may be at risk. Always check gloves 

before using them, and replace any that are 

damaged or otherwise unsuitable for use.

Risk and Safety Information (R and S phrases) 

The properties of all Merck products are closely scrutinized. Items classed as 

hazardous are marked as such in accordance with EU regulations. 

These prescribe labelling with hazard symbols and plain-text warnings, and the 

inclusion of risk and safety (R and S) information. 

It must, however, be noted that, while the data reflect the state at their 

generation, labels are continually updated to reflect the latest state of the art 

and detail changes in regulations. Should data differ those given on the label 

shall apply. 

Attention: In the case of R phrases with the addition E, 

the text starts with the word also. 

Example: R 20 harmful by inhalation. 

R E 20 also harmful by inhalation. 

Hint: These phrases are not listed below. 

Risk information (R phrases) 

R 1 Explosive when dry. 

R 2 Risk of explosion by shock, friction, fire 

or other sources of ignition. 

R 3 Extreme risk of explosion by shock, 

friction, fire or other sources of 

ignition. 

R 4 Forms very sensitive explosive metallic 

compounds. 

R 5 Heating may cause an explosion. 

R 6 Explosive with or without contact with 

air. 

R 7 May cause fire. 

R 8 Contact with combustible material 

may cause fire. 

R 9 Explosive when mixed with combustible 

material. 

R 10 Flammable. 

R 11 Highly flammable. 

R 12 Extremely flammable. 

R 14 Reacts violently with water. 

R 15 Contact with water liberates 

extremely flammable gases. 

R 16 Explosive when mixed with oxidizing 

substances. 

R 17 Spontaneously flammable in air. 

R 18 In use, may form flammable / 

explosive vapour-air mixture. 

R 19 May form explosive peroxides. 

R 20 Harmful by inhalation. 

R 21 Harmful in contact with skin. 

R 22 Harmful if swallowed. 

R 23 Toxic by inhalation. 

R 24 Toxic in contact with skin. 

R 25 Toxic if swallowed. 

R 26 Very toxic by inhalation. 

R 27 Very toxic in contact with skin. 

R 28 Very toxic if swallowed. 

R 29 Contact with water liberates toxic gas. 

R 30 Can become highly flammable in use. 

R 31 Contact with acids liberates toxic gas. 

R 32 Contact with acids liberates very toxic 

gas. 

R 33 Danger of cumulative effects. 

R 34 Causes burns. 

R 35 Causes severe burns. 

R 36 Irritating to eyes. 

R 37 Irritating to respiratory system. 

R 38 Irritating to skin. 

R 39 Danger of very serious irreversible 

effects. 

R 40 Limited evidence of a carcinogenic 

effect. 

R 41 Risk of serious damage to eyes. 

R 42 May cause sensitization by inhalation. 

R 43 May cause sensitization by skin 

contact. 

R 44 Risk of explosion if heated under 

confinement. 

R 45 May cause cancer. 

R 46 May cause heritable genetic damage. 

R 48 Danger of serious damage to health by 

prolonged exposure. 

R 49 May cause cancer by inhalation. 

R 50 Very toxic to aquatic organisms. 

65

66 Appendix 

R 51 Toxic to aquatic organisms. 

R 52 Harmful to aquatic organisms. 

R 53 May cause long-term adverse 

effects in the aquatic environment. 

R 54 Toxic to flora. 

R 55 Toxic to fauna. 

R 56 Toxic to soil organisms. 

R 57 Toxic to bees. 

R 58 May cause long-term adverse 

effects in the environment. 

R 59 Dangerous for the ozone layer. 

R 60 May impair fertility. 

R 61 May cause harm to the unborn 

child. 

R 62 Possible risk of impaired fertility. 

R 63 Possible risk of harm to the unborn 

child. 

R 64 May cause harm to breastfed babies. 

R 65 Harmful: May cause lung damage if 

swallowed. 

R 66 Repeated exposure may cause skin 

dryness or cracking. 

R 67 Vapours may cause drowsiness and 

dizziness. 

R 68 Possible risks of irreversible effects. 

Combination of R phrases 

R 14/15 Reacts violently with water, liberating 

extremely flammable gases. 

R 15/29 Contact with water liberates toxic, 

extremely flammable gas. 

R 20/21 Harmful by inhalation and in 

contact with skin. 

R 20/22 Harmful by inhalation and if 

swallowed. 

R 20/21/22 Harmful by inhalation, in contact 

with skin and if swallowed. 

R 21/22 Harmful in contact with skin and if 

swallowed. 

R 23/24 Toxic by inhalation and in contact 

with skin. 

R 23/25 Toxic by inhalation and if 

swallowed. 

R 23/24/25 Toxic by inhalation, in contact with 

skin and if swallowed. 

R 24/25 Toxic in contact with skin and if 

swallowed. 

R 26/27 Very toxic by inhalation and in 


R 26/28 Very toxic by inhalation and if 

swallowed. 

R 26/27/28 Very toxic by inhalation, in contact 

with skin and if swallowed. 

R 27/28 Very toxic in contact with skin and if 

swallowed. 

R 36/37 Irritating to eyes and respiratory 

system. 

R 36/38 Irritating to eyes and skin. 

R 36/37/38 Irritating to eyes, respiratory system 

and skin. 

R 37/38 Irritating to respiratory system and 

skin. 

R 39/23 Toxic: danger of very serious 

irreversible effects through 

inhalation. 


irreversible effects in contact 

with skin. 


irreversible effects if swallowed. 

R 39/23/24 Toxic: danger of very serious 


inhalation and in contact with skin. 



inhalation and if swallowed. 


irreversible effects in contact with 


R 39/23/24/25 Toxic: danger of very serious 


inhalation, in contact with skin and 

if swallowed. 

R 39/26 Very toxic: danger of very serious 


inhalation. 



skin. 


irreversible effects if swallowed. 

R 39/26/27 Very toxic: danger of very serious 


inhalation and in contact with skin. 



inhalation and if swallowed 



skin and if swallowed.

R 39/26/27/28 Very toxic: danger of very serious 

irreversible effects through inhalation, 

in contact with skin and if 

swallowed. 

R 42/43 May cause sensitization by 

inhalation and skin contact. 

R 48/20 Harmful: danger of serious damage 

to health by prolonged exposure 

through inhalation. 


to health by prolonged exposure in 



to health by prolonged exposure if 

swallowed. 

R 48/20/21 Harmful: danger of serious damage 


through inhalation and in contact 

with skin. 



through inhalation and if 

swallowed. 



contact with skin and if swallowed. 

R 48/20/21/22 Harmful: danger of serious damage 


through inhalation, in contact with 


R 48/23 Toxic: danger of serious damage 


through inhalation. 





to health by prolonged exposure if 

swal-lowed. 

R 48/23/24 Toxic: danger of serious damage 


through inhalation and in contact 

with skin. 

R 48/23/25 Toxic: danger of serious damage 


through inhalation and if 

swallowed. 

R 48/24/25 Toxic: danger of serious damage to 

health by prolonged exposure in 


R 48/23/24/25 Toxic: danger of serious damage to 

health by prolonged exposure 

through inhalation, in contact with 


R 50/53 Very toxic to aquatic organisms, 

may cause long-term adverse 

effects in the aquatic environment. 

R 51/53 Toxic to aquatic organisms, may 

cause long-term adverse effects in 

the aquatic environment. 

R 52/53 Harmful to aquatic organisms, may 

cause long-term adverse effects in 

the aquatic nvironment. 

R 68/20 Harmful: possible risk of irreversible 

effects through inhalation. 

R68/20/21 Harmful: possible risk of irreversible 

effects through inhalation and in 


R 68/20/21/22 Harmful: possible risk of irreversible 

effects through inhalation, in 



effects through inhalation and if 

swallowed. 

R68/21 Harmful: possible risk of irreversible 

effects in contact with skin. 


effects in contact with skin and if 

swallowed. 

R 68/22 Harmful: possible risk of irreversible 

effects if swallowed. 

Safety information (S phrases) 

S 1 Keep locked up. 

S 2 Keep out of the reach of children. 

S 3 Keep in a cool place. 

S 4 Keep away from living quarters. 

S 5 Keep contents under ...(appropriate 

liquid to be specified by the 

manufacturer). 

S 5.1 Keep contents under water. 

S 5.2 Keep contents under petroleum. 

S 5.3 Keep contents under paraffin oil. 

S 6 Keep under ...(inert gas to be 

specified by the manufacturer). 

S 6.1 Keep under nitrogen. 

S 6.2 Keep under argon. 

S 7 Keep container tightly closed. 

S 8 Keep container dry. 

67

68 Appendix 

S 9 Keep container in a well-ventilated 

place. 

S 12 Do not keep the container sealed. 

S 13 Keep away from food, drink and 

animal feeding stuffs. 

S 14 Keep away from (incompatible 

materials to be indicated by the 


S 14.1 Keep away from reducing agents, 

heavy metal compounds, acids and 

alkalis. 

S 14.2 Keep away from oxidizing and acidic 

substances as well as heavy metal 

compounds. 

S 14.3 Keep away from iron. 

S 14.4 Keep away from water and alkalis. 

S 14.5 Keep away from acids. 

S 14.6 Keep away from alkalis. 

S 14.7 Keep away from metals. 

S 14.8 Keep away from oxidizing and acidic 

substances. 

S 14.9 Keep away from flammable organic 

substances. 

S 14.10 Keep away from acids, reducing agents 

and flammable material. 

S 14.11 Keep away from flammable material. 

S 15 Keep away from heat. 

S 16 Keep away from sources of ignition - 

No smoking. 

S 17 Keep away from combustible material. 

S 18 Handle and open container with care. 

S 20 When using do not eat or drink. 

S 21 When using do not smoke. 

S 22 Do not breathe dust. 

S 23 Do not breathe gas/fumes/vapour/ 

spray (appropriate wording to be 


S 23.1 Do not breathe gas. 

S 23.2 Do not breathe vapour. 

S 23.3 Do not breathe spray. 

S 23.4 Do not breathe fumes. 

S 23.5 Do not breathe vapour/spray. 

S 24 Avoid contact with skin. 

S 25 Avoid contact with eyes. 

S 26 In case of contact with eyes, rinse 

immediately with plenty of water and 

seek medical advice. 

S 27 Take off immediately all contaminated 

clothing. 

S 28 After contact with skin, wash 

immediately with plenty of ... (to be 


S 28.1 After contact with skin, wash 

immediately with plenty of water. 

S 28.2 After contact skin, wash immediately 

with plenty of soap and water. 


immediately with plenty of soap and 

water, if possible also with polyethylene 

glycol 400. 


immediately with plenty of polyethylene 

glycol 300 and ethanol (2:1) 

followed by plenty of soap and water. 



glycol 400. 



glycol 400, then rinse with 

plenty of water. 


immediately with plenty of water and 

acidic soap. 

S 29 Do not empty into drains. 

S 30 Never add water to this product. 

S 33 Take precautionary measures against 

static discharges. 

S 35 This material and its container must be 

disposed of in a safe way. 

S 36 Wear suitable protective clothing. 

S 37 Wear suitable gloves. 

S 38 In case of insufficient ventilation, 

wear suitable respiratory equipment. 

S 39 Wear eye/face protection. 

S 40 To clean the floor and all objects 

contaminated by this material use ... 

(to be specified by the manufacturer). 

S 40.1 To clean the floor and all objects 

contaminated by this material use 

plenty of water. 

S 41 In case of fire and/or explosion do not 

breathe fumes. 

S 42 During fumigation/spraying wear 

suitable respiratory equipment 

(appropriate wording to be specified 

by the manufacturer).

S 43 In case of fire, use ... (indicate the 

precise type of fire-fighting 

equipment. If water increases risk, 

add - 'never use water'). 

S 43.1 In case of fire, use water. 

S 43.2 In case of fire, use water or powder 

extinguisher. 

S 43.3 In case of fire, use powder extinguisher 

- never use water. 

S 43.4 In case of fire, use carbon dioxide - 

never use water. 

S 43.6 In case of fire, use sand - never use 

water. 

S 43.7 In case of fire, use metal fire powder - 

never use water. 

S 43.8 In case of fire, use sand, carbon 

dioxide or powder extinguisher - never 

use water. 

S 45 In case of accident or if you feel 

unwell, seek medical advice 

immediately (show the label where 

possible). 

S 46 If swallowed, seek medical advice 

immediately and show this container 

or label. 

S 47 Keep at temperature not exceeding 

... °C (to be specified by the 


S 47.1 Keep at temperature not exceeding 

25 °C. 

S 48 Keep wet with ... (appropriate material 

to be specified by the manufacturer). 

S 48.1 Keep wet with water. 

S 49 Keep only in the original container. 

S 50 Do not mix with ... (to be specified by 

the manufacturer). 

S 50.1 Do not mix with acids. 

S 50.2 Do not mix with alkalis. 

S 50.3 Do not mix with strong acids, strong 

bases, non-ferrous metals or their 

salts. 

S 51 Use only in well-ventilated areas. 

S 52 Not recommended for interior use on 

large surface areas. 

S 53 Avoid exposure - obtain special 

instructions before use. Restricted to 

professional users. 

S 56 Dispose of this material and its 

container at hazardous or special 

waste collection point. 

S 57 Use appropriate container to avoid 

environmental contamination. 

S 59 Refer to manufacturer/supplier for 

information on recovery/recycling. 

S 60 This material and its container must be 

disposed of as hazardous waste. 

S 61 Avoid release to the environment. 

Refer to special instructions / Safety 

data sheets. 

S 62 If swallowed, do not induce vomiting: 

seek medical advice immediately and 

show this container or label. 

S 63 In case of accident by inhalation: 

remove casualty to fresh air and keep 

at rest. 

S 64 If swallowed, rinse mouth with water 

(only if the person is conscious). 

Combination of S phrases 

S 1/2 Keep locked up and out of reach of 

children. 

S 3/7 Keep container tightly closed in a 

cool place. 

S 3/9/14 Keep in a cool, well-ventilated place 

away from ...3/9 

S 3/9/14.1 Keep in a cool, well-ventilated place 

away from reducing agents, heavy 

metal compounds, acids and alkalis. 


away from oxidizing agents and 

acidic substances as well as heavy 

metal com-pounds. 


away from iron. 


away from water and alkalis. 


away from acids. 


away from alkalis. 


away from metals. 


away from oxidizing and acidic 

substances. 

S 3/9/14/49 Keep only in the original container 

in a cool, well-ventilated place away 

from ... (incompatible materials to 

be indicated by the manufacturer). 

69

70 Appendix 

S 3/9/14.1/49 Keep only in the original container 


from reducing agents, heavy metal 

compounds, acids and alkalis. 



from oxidizing and acidic 

substances as well as heavy metal 

compounds. 



from iron. 



from water and alkalis. 



from acids. 



from alkalis. 



from metals. 



from oxidizing and acidic 

substances. 

S 3/9/49 Keep only the original container in a 

cool, well-ventilated place. 

S 3/14 Keep in a cool place away from ... 

(incompatible materials to be 

indicated by the manufacturer). 

S 3/14.1 Keep in a cool place away from 

reducing agents, heavy metal 

compounds, acids and alkalis. 


oxidizing and acidic substances as 

well as heavy metal compounds. 

S 3/14.3 Keep in a cool place away from iron. 


water and alkalis. 


acids. 


alkalis. 


metals. 


oxidizing and acidic substances. 

S 7/8 Keep container tightly closed and 

dry. 

S 7/9 Keep container tightly closed and in 

a well-ventilated place. 

S 7/47 Keep container tightly closed and at 

a temperature not exceeding ... °C 

(to be specified by the 


S 20/21 When using do not eat, drink or 

smoke. 

S 24/25 w Avoid contact with skin and eyes. 

S 27/28 w After contact with skin, take off 

immediately all contaminated 

clothing, and wash immediately 

with plenty of . . . 

S 29/35 w Do not empty into drains; dispose of 

this material and its container in a 

safe way. 

S 29/56 w Do not empty into drains, dispose of 

this material and its container at 

hazard-ous or special waste 

collection point. 

S 36/37 Wear suitable protective clothing 

and gloves. 

S 36/37/39 Wear suitable protective cloth-ing, 

gloves and eye/face protection. 

S 36/39 Wear suitable protective clothing 

and eye/face protection. 

S 37/39 Wear suitable gloves and eye/ face 

protection 

S 47/49 Keep only in the original container 

at a temperature not exceeding ... °C 

(to be specified by the 

manufacturer).

Poster - Safety in the Laboratory - 

This poster is conveniently divided into various 

sections and is a suitable vehicle for imparting 

safety information. The poster Safety in the 

laboratory contains all information relevant to 

the laboratory and the warehouse, such as 

· safety rules 

· storage classes 

· hazard symbol descriptions 

· risk and safety phrases 

· well as a list of hazardous chemicals with their 

respective risk and safety classifications 

Patent and Licence Information 

Disclaimer 

We provide information and advice to our customers 

on application technologies and regulatory 

matters to the best of our knowledge and 

ability, but without obligation or liability. 

Existing laws and regulations are to be 

observed in all cases by our customers. This 

also applies in respect to any rights of third 

parties. Our information and advice do not 

relieve our customers of their own responsibility 

for checking the suitability of our products 

for the envisaged purpose. 

Being in portrait format (68 x 100 cm), it can 

be easily affixed to a fairly narrow expanse of 

wall. Please note that, for safety reasons, it 

must not be attached to glass panes on doors. 

Order information: 

Poster – Safety in the Laboratory – 

Order No. 4.03089.0001 

Benzonase® is covered by several patents, i.e. 

US Patent No.5,173,418 

EP Patent No.0229866 B1 

Nycomed Pharma A/S, Denmark claims all world-wide patent rights to Benzonase®. 

Merck KGaA is holding an exclusive licence on all patents. 

Trademak Information 

Trademarks of Merck KGaA 

Benzonase® 

Extran® 

Fractogel® 

Fractoprep® 

Hibar® 

LiChroprep® 

LiChrospher® 

Lobar® 

Pharmprep® 

Polysphere® 

Other trademarks 

Avicel® = trademark of FMC, Corp. USA 

Brij® = trademark of ICI America, Inc. USA 

Florisil® = trademark of Floridin Co., USA 

Teflon® = trademark of DuPont, USA 

Triton® = trademark of Union Carbide, USA

EMD Chemicals Inc. 

480 S. Democrat Road 

Gibbstown, NJ 08027 

Tel: +1 888 234 3228 

Fax: +1 856 224 0742 

pharma@emdchemicals.com 

We provide information and advice to our customers to the best of our knowledge and ability, but without obligation or liability. Existing laws and 

regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not 

relieve our customers of their own responsibility for checking the suitability of our products for the envisaged purpose. 

Fractogel® and Fractoprep® are registered trademarks of Merck KGaA, Darmstadt, Germany.

Processing Catalog - EMD Chemicals

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