project honey as antimicrobial agent final full.pdf - University of ...

2.6.1.7 Staining and gel visualising: 77
2.7 Statistical analysis of the data: 78
Chapter 3: Results 79
3.1 Confirmations of the identity and antibiotics sensitivities of test
organisms:
3.2 Characterization of honey samples: 88
3.2.1 Determination of antibacterial activity: 88
3.2.2 Chemical & physical analysis of honey samples 91
3.2.2.1 Pollen analysis 94
3.2.2.2 Antioxidant activity assay: 96
3.3 Determination of antibacterial activity of honey samples against test
cultures:
3.3.1 Minimum Inhibitory Concentration (MIC) and Minimum
Bactericidal Concentration (MBC) of manuka honey:
3.3.1.1 Agar incorporation method: 97
3.3.1.2 Broth dilution method: 99
3.3.2 Sensitivity of MDR and ESBLs to Omani honey: 107
3.4 Time Kill Curves 115
3.4.1 Inhibition of test organisms by manuka honey monitored by
optical density:
3.4.2 Inhibition of test organisms by manuka honey monitored by total 119
8
80
97
97
115