Confocal Microscopy Principles

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How big is a Laser Scanning <strong>Confocal</strong> Laser module 405, 458, 477, 488, 514, 561, 633 nm System electronic rack Microscope ? Scanning head
LASER Light Amplification by Stimulated Emission of Radiation • High intensity • Spatial and temporal coherence • Monochromatic • Focused Lasers installed in our Laser Scanning Microscopes • 405 nm Diode laser (DAPI, CFP…) • Argon ion gas laser with 458 nm (CFP…) 488 nm (FITC, GFP, Alexa 488 …) 514 nm (YFP…) • Helium neon 543 nm gas laser (TRITC, Cy3, Alexa 546 …) • 561 nm DPSS laser (Texas red, Alexa 568 …) • Helium neon 633 nm gas laser (TOTO3, Cy5 …)
Page 1 and 2: Confocal Microscopy Dr. Serge Arnau
Page 3 and 4: Light source in focus Sample (objec
Page 5 and 6: Light source Confocal microscope pr
Page 7 and 8: How does a fluorescence microscope
Page 9: Advantage of fluorescence confocal
Page 13 and 14: Beam scanning Majority of laser sca
Page 15 and 16: Photomultiplier Tubes (PMT) The spe
Page 17 and 18: Scans averaging reduces noise Avera
Page 19 and 20: Airy disk and Resolution The radius
Page 21 and 22: Pinhole and Resolution Confocal pin
Page 23 and 24: Pinhole and Resolution Confocal PSF
Page 25 and 26: Z Optical sectionning Y X 5 4 3 2 1
Page 27 and 28: Sampling in confocal microscopy 260
Page 29 and 30: Sampling in confocal microscopy The
Page 31 and 32: Ideal emission separation Red emiss
Page 33 and 34: Crosstalk problems To avoid bleed-t
Page 35 and 36: Spectral separation Fluorescence se
Page 37 and 38: FRAP experiments FRAP-recording for
Page 39 and 40: Other beam scanning techniques Mult
Page 41 and 42: Nipkow disk confocal microscope fac
Page 43 and 44: Other beam scanning techniques Slit

Confocal Microscopy Principles

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