Confocal Microscopy Principles
Confocal Microscopy Principles
Confocal Microscopy Principles
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Very high control of the scanner<br />
by the DSP (Digital Signal<br />
Processor) to position the laser<br />
beam and choose ROI of any<br />
shape<br />
Photobleaching<br />
Bovine endothelial cells<br />
actin filaments (BODIPY FL),<br />
mitochondria (MitoTracker Red);<br />
some mitochondria are marked<br />
for photobleaching<br />
Bleaching of marked mitochondria with pinpoint<br />
accuracy (left)<br />
Merged images of mitochondria before and after<br />
photobleaching :bleached portions appeared in red<br />
(right)
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