Confocal Microscopy Principles
Confocal Microscopy Principles
Confocal Microscopy Principles
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Sampling in confocal microscopy<br />
What is the zooming factor limit?<br />
This is linked to the X,Y resolution of the optics<br />
Sampling is sufficient when there is enough pixels to<br />
separate two adjacent Airy disk<br />
Nyquist theorem :<br />
to reconstruct a sine wave : f sampling = 2 x f wave<br />
In imaging, frequency = spatial frequency<br />
f sampling = 2.3 x f highest (to compensate low-pass filtering)
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