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Details - CALS Networking Lab - University of Arizona

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near intensive plots, but most were in areas we<br />

thought would have high species richness, species<br />

<strong>of</strong> special interest, or species suspected to be in<br />

the district that had not previously been recorded<br />

(e.g., the rock rattlesnake). Extensive surveys<br />

were more flexible and allowed for variation in<br />

survey time and area. For road and extensive<br />

surveys, we surveyed in evenings and nights to<br />

detect species with restricted activity periods<br />

(Ivanyi et al. 2000). Although we designed<br />

methods to detect both amphibians and reptiles,<br />

we detected fewer amphibians because they<br />

have more limited activity periods and are <strong>of</strong>ten<br />

restricted to aquatic environments, which are rare<br />

in the district.<br />

Intensive Surveys<br />

Field Methods<br />

At focal-point transects (hereafter “transects”)<br />

in 2001, we used plot-based visual encounter<br />

surveys constrained by time and area (Crump<br />

and Scott 1994) along 17 transects (Figs. 4.1,<br />

4.2). Along each transect we surveyed within<br />

the confines <strong>of</strong> three 1-ha (100 x 100 m) subplots<br />

during spring (9 April - 24 May) or two subplots<br />

during the summer monsoon (18–31 July) and<br />

searched each subplot for one hour. We surveyed<br />

only two subplots in summer because there<br />

was not sufficient time during peak activity<br />

periods to search all three subplots. Although<br />

Focal<br />

Point<br />

Transect<br />

line<br />

1000 m<br />

28<br />

we surveyed all 17 transects in spring only seven<br />

transects were surveyed in summer and these<br />

were located only in low (n = 3) and middle (n<br />

= 4) elevation strata. We selected survey times<br />

that coincided with periods <strong>of</strong> peak diurnal<br />

reptile activity because activity levels vary with<br />

temperature (Rosen 2000). On cooler spring days<br />

we began our surveys between 0718 and 1421<br />

hours whereas on hotter, summer days we began<br />

between 0642 and 1014 hours. To account for<br />

within-day variation in detectability and to reduce<br />

variation among observers, we surveyed each<br />

subplot twice per day by a different observer. We<br />

did not survey during evenings or nights.<br />

We searched subplots visually and aurally<br />

and worked systematically across each subplot<br />

and used a Garmin E-map GPS to ensure we<br />

stayed within subplot boundaries during surveys.<br />

We also looked under rocks and litter and used<br />

a mirror to illuminate cracks and crevices. For<br />

each animal detected, we recorded species, sex<br />

and age/size class (if known), and microhabitat<br />

(ground, vegetation, rock, edifice, burrow, or<br />

water). We marked subplot corners with rubbercapped<br />

stakes and recorded UTM coordinates<br />

with a Trimble GPS. We recorded temperature,<br />

wind speed (km/h), percent relative humidity,<br />

and percent cloud cover using hand-held Kestrel<br />

3000 weather meters (Nielson-Kellerman Inc.,<br />

Boothwyn, PA) before and after surveys. We also<br />

described vegetation and soils.<br />

Plot Number<br />

1 2 3 4 5 6 7 8 9 10<br />

Figure 4.1. Layout <strong>of</strong> herpet<strong>of</strong>auna survey plots along focal-point transects, Saguaro National Park,<br />

Rincon Mountain District, 2001. We typically surveyed three, 100 x 100 m subplots (dotted boxes) in the<br />

spring and two subplots (1 and 10) in the summer. When topography prevented surveys in a subplot, we<br />

surveyed an adjacent subplot.<br />

100 m<br />

100 m

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