Systems

Application:
In many cases a serum-free cultivation can be carried out without time-consuming adaption steps.
With critical clones, reduce the serum slowly (see instruction PANSERIN TM 401).
• Warm up PANSERIN TM PX10 to 37 °C.
• Transfer hybridoma directly into PANSERIN TM PX10. In most cases a cell number of 1000 cells/ml
is sufficient.
• Incubate the cells in the usual way in the CO2-incubator at 37 °C (5 % CO2-fumigation) res. multiply
them in the bioreactor.
• Extract monoclonal antibodies from the supernatant.
Order Information
Product: suitable for: Quantity: Cat.-No.:
PANSERIN TM PX10 serumfree medium for 500 ml P04-710PX10
myeloma cells, production 100 ml P04-710PX10M
of monoclonal Antibodies
BIOTECH GmbH
The company
Am Gewerbepark 13, D-94501 Aidenbach
phone +49 (0) 85 43 - 60 16-30, fax +49 (0) 85 43 - 60 16-49
e-mail: info@pan-biotech.de, http://www.pan-biotech.de
21
Typical growth curve
of SP2/0-Ag-14
in PANSERIN TM PX10
Sp2/0-Ag-14 cells have been
transferred from a culture
containing serum (RPMI 1640
with 10 % FCS) directly into
PANSERIN TM PX10.
Seed 1000 Zellen/ml. As a
comparison Sp2/0-Ag-14
in RPMI 1640 with 10 % FCS.
SP2/0-Ag-14 cells were
used from a culture
containing serum directly
in a cloning efficiency test
with PANSERIN TM PX10
The absolute cloning efficiency
was 75 % (77 % with 20 % FCS)
the relative cloning efficiency
97 % (100 % with 20 % FCS)