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Fully-Automatic Cell Culture System from PAN Incubation chambers with connections for media and gas supply CO2-supply Heating plate Glass top for microscopic observation BIOTECH GmbH 4 Media supply Digital camera Incubation chambers Control heating CO2-fumigation Image processing In the following example, CHO-cells were cultivated in DMEM with 4,5 g/l glucose with L-glutamine, with pyruvate and different serum substitutes (seed 1 x 10 4 /ml, evaluation 7th day, cells not adapted). CHO with serum substitute 1 CHO with serum substitute 2 CHO with 5 % Panexin Cells do not form any Cells do not form any Cells form uniform cell layer, loose attachment cell layer, loose attachment cell layer The company Am Gewerbepark 13, D-94501 Aidenbach phone +49 (0) 85 43 - 60 16-30, fax +49 (0) 85 43 - 60 16-49 e-mail: info@pan-biotech.de, http://www.pan-biotech.de
In order to satisfy our customers’ wishes, we will continue to expand and optimize our range of serum- and proteinfree media. Beside nutrient solutions ready for use (PANSERIN TM ) we can also offer serum substitutes (PANEXIN) to our customers which are added in a concentration of 2 % - 8 % instead of the serum to the respective basic media (e.g. RPMI 1640, DMEM, DMEM/F12). Serumfree nutrient media without animal or human components res. proteinfree media are also already available at PAN-Biotech res. in development. In many cases an adaption of the cells to the serumfree culture is not necessary; with critical cells (primary cells) the cells are slowly familiarized with the serumfree conditions with help of our detailed records. Product Survey Product: suitable for: Quantity: Cat.-No.: Page: PANSERIN TM 401 serumfree allround medium 500 ml P04-710401 7-9 Serumfree allround for many cell types 100 ml P04-710401M medium PANSERIN TM 411 serumfree allround medium 500 ml P04-710411 7-9 Serumfree allround for cells which also need Insulin 100 ml P04-710411M medium with Insulin PANSERIN TM 412 serumfree allround medium 500 ml P04-710412 7-9 Serumfree allround for cells that hardly attach 100 ml P04-710412M medium with Fetuin PANSERIN TM 413 serumfree cultivation 500 ml P04-710413 10-11 Growth factors of T-cells mixture separate PANSERIN TM 415 serumfree cultivation 500 ml P04-710415 12 of Osteoblasts 100 ml P04-710415M PANSERIN TM 416 Kit serumfree cultivation 500 ml P04-710416 13-15 Basic medium with of DC (Dendritic Cells) 100 ml P04-710416M growth factor set BIOTECH GmbH The company Am Gewerbepark 13, D-94501 Aidenbach phone +49 (0) 85 43 - 60 16-30, fax +49 (0) 85 43 - 60 16-49 e-mail: info@pan-biotech.de, http://www.pan-biotech.de 5
Page 1 and 2: Serumfree Systems Serumfree Systems
Page 3: Advantages of a Serumfree Cell Cult
Page 7 and 8: PANSERIN TM 401 PANSERIN TM 401 is
Page 9 and 10: Order Information: BIOTECH GmbH 9
Page 11 and 12: Order Information: Product: suitabl
Page 13 and 14: PANSERIN TM 416 PANSERIN TM 416 is
Page 15 and 16: Serumfree Cultivation of Dentritic
Page 17 and 18: PANSERIN TM 604S without animal or
Page 19 and 20: PANSERIN TM PX10 PANSERIN TM PX10 i
Page 21 and 22: Application: In many cases a serum-
Page 23 and 24: • Harvest cells in the usual way.

Systems

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