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Microscopic X-ray fluorescence analysis of Spheroid Culture
Model of Human Prostate Cells
Leitão,R.G. 1 , Santos, C. A. N. 2 , Palumbo, A.J 1 , Souza, P.A.V.R 1 , Pereira, G.
R. 1 , Canellas,C.G.L 1 , Anjos, M. J. 3 , Nasciutti, L.E 1 , and Lopes, R.T. 1
1 Universidade Federal do Rio de Janeiro - Rio de Janeiro RJ Brazil
2 Instituto Nacional de Metrologia, Normal e Qualidade Industr - Xerém RJ Brazil
3 Universidade do Estado do Rio de Janeiro - Rio de Janeiro RJ Brazil
Diseases of the prostate gland such as Prostate cancer (PCa) and Benign prostate
hyperplasia (BPH) are the most frequently health problems in men past middle
age. Spheroid Culture Model are a useful culture technique for tissue engineering
or regenerative medicine research, pharmacological and toxicological studies, and
fundamental studies in cell biology. Synchrotron Radiation micro X-Ray Fluorescence
(µSRXRF) was used to investigate two-dimensional spatial distributions of
Fe, Cu and Zn in spheroids derived from prostate tissue with PCa and BPH. The
measurements were carried out in the X-ray microfluorescence station of the XRF
beam line at the Synchrotron Light National Laboratory (LNLS). The distribution
of the elements Fe, Cu and Zn was determined. The results showed that the
elements analyzed presented non-uniform distribution.
Acknowledgements: This work was partially developed at Brazilian National Synchrotron
Light Laboratory (projects XRF 10894 and 11872) and had the financial support of CNPq
and FAPERJ.
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