role of th1 and th17 cd4+ t cell subsets in the pathogenesis of ...

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4 5.2 ‐ Results ..........................................................................................................................122 5.2.1 Identification of Rev‐Erbα and its expression in CD4 + T cell subsets .....................122 5.2.2 Relevance of Rev‐Erbα in EAE and Th17 differentiation .......................................126 5.3 ‐ Discussion and Conclusions ..........................................................................................131 CHAPTER 6 GENERAL DISCUSSION AND MAIN CONCLUSIONS ...........................................135 Reference List ........................................................................................................................141 Notes ........................................................................................................................155
ABBREVIATIONS aa – Amino acid ADCC – Antibody dependent complement cytolysis Ahr – Aryl hydrocarbon receptor APC – Allophycocyanin APCs – Antigen presenting cells BBB – Blood brain barrier BCR – B cell receptor CCL / CXCL – Chemokine ligands CCR / CXCR – Chemokine receptor CD – Cluster differentiation CFA – Complete Freund´s adjuvant DBD – DNA binding domain CNS – Central nervous system CSF – Cerebrospinal fluid CTLA‐4 – Cytotoxic T lymphocyte‐associated antigen 4 DCs – Dendritic cells EAE – Experimental autoimmune encephalomyelitis EAU – Experimental autoimmune uveitis ELISA – Enzyme linked immunosorbent assay FACS – Fluorescence‐activated cell sorting FITC – Fluorescein isothiocyanate Foxp3 – Forkhead box P3 GATA‐3 – GATA binding protein 3 GFAP – Glial fibrillary acidic protein GFP – Green fluorescent protein GM‐CSF ‐ Granulocyte‐macrophage colony‐stimulating factor HLA – human leukocyte antigen IFN – Interferon Igs – Immunoglobulins IL – Interleukin IRES – internal ribosome entry site iTregs – Induced regulatory T cells LBD – Ligand binding domain MAG – Myelin‐associated glycoprotein 5
Page 1: Helena Sofia de Azevedo Domingues T
Page 4 and 5: Ferreira e Ricardo Marques pela mot
Page 7 and 8: TABLE OF CONTENTS Acknowledgments /
Page 9: 2.2.13 Quantitative real‐time PCR
Page 13: PUBLICATIONS During the development
Page 17 and 18: SUMMARY Multiple sclerosis (MS) is
Page 19 and 20: RESUMO A Esclerose Múltipla (EM)
Page 21: OBJECTIVES The main aim of this the
Page 25 and 26: CHAPTER 1 ‐ INTRODUCTION Role of
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Page 59 and 60: 2.1 - MATERIALS 2.1.1 BUFFERS AND R
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CHAPTER 2 ‐ METHODS Role of Th1 a
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GM‐CSF Granzyme A Granzyme B IFN
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CHAPTER 3 IN VIVO ANALYSIS OF TH1 A
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Our experiments make several points
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Additionally, IL‐4 and IL‐5 wer
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3.4a, b). In addition, we consisten
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The CNS infiltrating mononuclear ce
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Histology and immunohistochemistry
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and brain stem and cerebellum more
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CHAPTER 3 - IN VIVO ANALYSIS OF TH1
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CHAPTER 4 EVALUATION OF CYTOTOXIC P
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4.1 - SUMMARY CHAPTER 4 - EVALUATIO
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CHAPTER 4 - EVALUATION OF CYTOTOXIC
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CHAPTER 5 MICROARRAY ANALYSIS OF T
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5.1 ‐ SUMMARY CHAPTER 5 -MICROARR
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CHAPTER 5 -MICROARRAY ANALYSIS OF T
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CHAPTER 6 GENERAL DISCUSSION AND MA
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CHAPTER 6 - GENERAL DISCUSSION AND
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CHAPTER 6 - GENERAL DISCUSSION AND
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REFERENCE LIST Abromson‐Leeman S,
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REFERENCE LIST Role of Th1 and Th17
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NOTES
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NOTES Role of Th1 and Th17 CD4 + T
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NOTES Role of Th1 and Th17 CD4 + T
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