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SCIENCE TEST

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The word tared is italicized and defined in the description of Experiment 1. This is a clue<br />

that it will be used in one of the questions (we saw the same thing with the word<br />

refracted in Passage I). The definition given for tared is “the balance was reset to 0.000<br />

g)”. Why would the students reset the balance to 0 after the graduated cylinder was<br />

placed on it? Because they are interested in the weight of the liquid added to the cylinder,<br />

not the weight of the liquid plus the weight of the cylinder.<br />

27. A student claimed that polycarbonate is more dense than PA-6. Do the results of Experiments 1−3<br />

support his claim?<br />

A. No, because in Liquid 8, polycarbonate stayed at the bottom and PA-6 rose.<br />

B. Yes, because in Liquid 8, polycarbonate stayed at the bottom and PA-6 rose.<br />

C. No, because in Liquid 8, polycarbonate rose and PA-6 stayed at the bottom.<br />

D. Yes, because in Liquid 8, polycarbonate rose and PA-6 stayed at the bottom.<br />

4<br />

Answer: B.<br />

From Table 3, we see Passage that polycarbonate VI and PA-6 are both plastics used to make Experiment beads 2<br />

for Experiment 3. If polycarbonate sinks in a particular liquid and PA-6 rises in that Synergism occurs when 2<br />

Bacteria break down sugars by fermentation. To study<br />

liquid, then polycarbonate is more dense that PA-6. This is exactly what happens together for to ferment a sugar by using<br />

2 fermentation pathways, researchers performed 2 experi-<br />

species can use alone. To investiga<br />

liquid 8.<br />

ments using broth that contained either the sugar sucrose or ment 1 was repeated, except that diff<br />

the sugar lactose. One of the fermentation pathways pro- species were added to each large test<br />

duces CO2 gas and increases the acidity (lowers the pH) of<br />

Passage VI the solution. The other pathway produces acid but not CO2. Bacteria break down sugars by fermentation. To study 2 fermentation pathways, researchers performed 2<br />

experiments using broth that contained either the sugar sucrose or the sugar lactose. One of the<br />

Table 2<br />

fermentation pathways produces CO2 gas and increases the acidity (lowers the pH) of the solution. The<br />

other pathway produces acid but not CO2. Experiment 1<br />

Sucrose broth<br />

Species<br />

Experiment 1<br />

Sucrose broth was added to 5 large test tubes. Next,<br />

added acid CO2 Sucrose broth was added phenol to 5 large red (a test pH tubes. indicator Next, phenol that is red yellow (a pH if indicator pH < 7, that red is ifyellow<br />

if pH < 7, red<br />

pH ≥ 7) was added to each large test tube. A Durham tube<br />

A and B − −<br />

if pH ≥ 7) was added to<br />

(a<br />

each<br />

small<br />

large<br />

test<br />

test<br />

tube)<br />

tube.<br />

was<br />

A Durham<br />

placed,<br />

tube<br />

inverted,<br />

(a small<br />

in<br />

test<br />

each<br />

tube)<br />

large<br />

was<br />

test<br />

placed, inverted, in<br />

A and C + +<br />

each large test tube to collect tube to CO collect 2 (see CO Figure 1). 2 (see Figure 1).<br />

B and D + +<br />

C and D + +<br />

Durham<br />

tube<br />

broth<br />

(red)<br />

Figure 1<br />

The large test tubes were capped, The large heated test until tubes the solutions were capped, were sterile, heated then until cooled. theOne<br />

of 4 bacterial<br />

species (Species A−D) solutions was added were to each sterile, of 4 of then the large cooled. test tubes. One The of 4 procedure bacterial was repeated using<br />

lactose broth instead of species sucrose (Species broth. The A−D) 10 large was test added tubes to (all each containing of 4 of the solutions large at a pH of 7) were<br />

then incubated at 37°C<br />

test<br />

for 48<br />

tubes.<br />

hr.<br />

The procedure was repeated using lactose broth<br />

instead of sucrose broth. The 10 large test tubes (all containing<br />

solutions at a pH of 7) were then incubated at 37°C<br />

for 48 hr.<br />

28. In Experiment 1, which of the<br />

The large test tubes and Durham tubes were exam- mented lactose?<br />

ined. If acid was produced, the solution was yellow. If no F. Species B only<br />

acid was produced, the solution remained red. If CO2 was G. Species C only<br />

produced, a gas bubble was observed at the top of the H. Species B and Species D on<br />

Durham tube (see Table 1).<br />

J. Species C and Species D on<br />

29. Suppose that in Experiment 2

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