Complete Issue - University of Oklahoma

DIS 75 (July 1994)
Mutation Notes - Drosophila melanogaster
Report of Regna Chorsky and John Belote.
Department of Biology, Syracuse University, Syracuse, NY 13244.
genitalia missing (gem): An autosomal recessive mutant that afects development of the genital dic derivatives
During the course of an X-ray mutagenesis screen designed to isolate mutants in the 73AD re~on (Belote, et al.,
1990), a deficiency of the hairy locus was dicovered as a chromosome that faied to complement the h2 alele prese!Jt on
the Df(3L)st-81k17 tester chromosome. Durg the intial characterition of this hairy deficiency, caed Df(3L)hI22, it
was noticed that when th chromosome was made heterozygous with the Df(3L)tra chromosome, a genitala-defective
phenotye was produced (e.g. al or parts of the genital disc derivatives were missing). The mutant present on the
Df(3L)tra chromosome responsible for thi phenotye was mapped by meiotic recombination to a position very near tle
h locus. Thi new mutant, named
genitalia missing (gem), is assumed to lie.withi the region deleted in the Df(3L)hi22
deficiency (i.e., 66D10-14). The phenotyes of gem/gem and gem/Df(3L)hI22 fles are similar, suggesting that the gem
alele is an amorph or a strong hypomorph. Penetrance of th mutant is high, but the expressivity is varable: some
mutant fles lack external genitala, whie others are missing only parts of the genital disc derivatives. Both males and
females are afected, although the phenotye in males is more conspicuous. In addition to the genitala defects, mutant
fles also show abnormal wig vein (LV is incomplete). Both of these phenotyes map to the same position and are
presumably due to the same genetic lesion.
Acknowledgments: The Df(3L)hi22 deficiency was isolated in a screen carried out in collaboration with Dr. M.
McKeown in the laboratory of Dr. B. Baker, UCSD. The Df(3L)st-81k17 chromosome was provided by Dr. M.M.
Green. This work was supported by a grant from the NIH.
Reference: Belote, J.M., F.M. Hoffman, M. McKeown, R. Chorsky, and B.S. Baker 1990, Genetics 125: 783-793.
Report of Zhimulev. I.F. Institute of Cyology and Genetics, Novosibirsk, 63090, Russia.
Drosophila melanogaster liage data.
In (3R)P23 gamma-rays (4.0 kR) (Solovjova) In (3R)90D;100Cl-2
In(l)A (received from R. Woodruf. In (1)9E7-8;13El-2. Homozygous viable.
In(1)AM (received from B. Rasmuson). In (1)8D6-12;16EF. Homozygous viable.
In (1)123 (received from D. Nash). In (1)9E1-1;20. Homozygous lethal.
In (1)BM2 (received from B. Rasmuson). In (1)
T(1;Y)B105 (received from J. Merriam). X-chromosome break is located in the middle part of
16A 1-6;20. Homozygous viable.
33
the band 10Al-2.
flw Mutation was found to be withi Df(1)HC133, Df(1)vL15, and Df(1)sbrK1 but not withi Df(1)ras-v17Ce8,
Df(1)vP5, Df(l)vLll, i.e., this mutation locaed in 9B9-9C4 interval.
ny Cyologicay located in 10B3-10B17 interval: withi Df(1)RA37, Df(1)N71, Df(1)KA7, not withi Df(1)vL3 and
Df( 1 )vL1. Both expressivity and penetrance are stronger at 18°C; more than 90% of fles have notches on both
margi of wigs at 18°C, and less than 40% at isc and 30°C (only in inner margins of wigs).
Report of Chatterjee. R.N.
Department of Zoology, University of Calcutta, 35 Balyguge Circular Road, Calcutta-700 019, India.
Alele of intersex (ix).
The intersex (ix) locus is one known sex determing mutant gene in D. melanogaster. It was dicovered by Morgan
et al. (1943). The phenotyes of mutations of ix/ix and ix2/;2 fles were also recorded by Lindsley and Grell (1968) and
Lindsley and Zimm (1985). The mutant ix is maitaied as BSY, pr en ix/SM5. Using the same stock, provided by Dr.
Rolf Nothiger, Zoologisches Institut der Universitat Zurich, Switzerland, I noted that, in some BS Y, ix/ix males, the