IVRI BT 3066.pdf

Directorate of Coldwater Fisheries Research
Artificial feed
The formulated diets used in cage culture
rearing trials contained crude protein (30% and
35%) and were with fishmeal and withoutfishmeaL
The feed ingredients used in feed formulation
included fishmeal, soybean meal, groundnut oil
cake, rice bran, wheat middling, vegetable oil,
vitamin and mineral mix.
Physico-chemical characteristics of
water
The important water quality parameters
assessed inside the cages ranged as: water
temperature, 15-22OC, dissolved oxygen 9.4-12
mgll, free carbon dioxide 0-1.0, total alkalinity
60-86 mgll and pH 7.2-8.2. The average values
for nitrate, nitrite, ammonium and phosphate were
Project Title
Personnel
3.0 mgfl, 0.03 mgfl, Nil and 0.005 mgfl,
respectively.
Conclusions
In cage culture bigger size group of golden
mahseer exhibited better growth performance and
feed efficiency when fed afishmeal fire diet having
30% dietary protein. While smaller size group of
golden mahseer expressed better growth
performance and feed efficiency when fed fishmeal
containing diet having 35% dietary protein.
Therefore, cage culture was found to be
suitable for rearing snow trout and golden mahseer
of smaller and bigger size groups. Moreover
bigger sized fishes can be cultured in cages, with
significant growth perfonnance, using fishmeal fire
diets.
Investigations on cold-water fish pathogens and their environment
Amit Pande, N.N Pandey and Yasmeen Basade
Setting up offish health laboratory was given
due attention and the lab was further furnished by
procuring more equipments namely a laminar flow,
autoclave, hot air oven and a table top refrigerated
centrifuge. Except for the tabletop refrigerated
centrifuge, all equipments have been installed. The
equipment procured in the financial year 2007-
08 were also installed namely the inverted
microscope (Nikon), horizontal gel
electrophoresis (Atto) and -80°C deep freezer
with CO 2 backup system (Sanyo). Besides these
equipments, the laboratory was also equipped
with minor equipments like a pH meter, a
microwave oven, a refrigerator and LPG
cylinders. Consumables like glassware and plastic
ware along with some more important media and
chemicals were also procured for the smooth
functioning of the laboratory.
Regular sampling was carried out during March
2008-Feb 2009 from the Institute's Fish Farm at
Chirapani, Champawat. Both water and diseased
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fish were sampled for isolation of fish pathogens.
Initially isolation of bacteria and fungi was
attempted as the facilities for observing the fish
parasites were not adequate. With the installation
of Nikon inverted microscope at Champawat
work on observation on fish parasites too was
initiated.
Isolation of bacterial fish pathogens was
attempted from both water and diseased fish. A
total of 994 isolates of bacteria were collected
from coldwater fish farms of Champawat with a
small fraction from Bairangana. Pure culture of
these isolates were maintained at -800C for further
characterization as at this stage the laboratory was
not having the necessary facilities. However,
biochemical characterization of some bacterial
isolates was attempted at CIFE using VITEK 2
ID-GNB cards. The equipment identified the
selected bacterial isolates as Aeromonas
hydrophila, Aeromonas sobria, Aeromonas
caviae, Pseudomonas luteola, Pseudomonas