Brian Dilkes Curriculum Vitae - Purdue University

Brian Dilkes
Curriculum Vitae
Purdue University Phone: (765) 494 2584
625 Agriculture Mall Dr eMail: bdilkes@purdue.edu
West Lafayette, IN 47907 Fax: (765) 494 0391
http://openwetware.org/wiki/User:Bdilkes
EDUCATION
2003 Ph. D., University of Arizona, Department of Plant Sciences
1993 B. L. A., Oberlin College, Biology
RESEARCH EXPERIENCE
2009- Assistant Professor, Department of Horticulture and Landscape
Architecture, Purdue University
2006-2008 Project Scientist, Genome Center, University of California, Davis
2003-2005 USDA Postdoctoral Fellow, Biology Dept., University of Washington
1994-2002 Graduate research assistant, Department of Plant Sciences, University of
Arizona
1992-1994 Undergraduate researcher and technician, Department of Plant Sciences,
University of Arizona
AWARDS
USDA-CREES-NRI 2003-35300-13248 “Functional genomic analysis of plant responses
to autopolyploidy” 2003-2005
Role: Project Director/PI
NSF Plant Genome Research Project grant DBI-0077774/DBI-0501712 “Functional
genomics of plant polyploids” 2005-2010
Role: Project Manager, PI Comai
NIH R01 GM076103-01A1 “Dosage-dependent regulation in hybridization” 2006-2011
Role: Senior Personnel, PI Comai
REFEREED PUBLICATIONS
Henry IM, Dilkes BP, Tyagi A, Lin H-S, and Comai L Chromosome number dynamics in
Arabidopsis thaliana. In Revision
Josefsson C, Dilkes BP, Kozloff N, Torjek O, Meyer R, Altmann T and Comai L Hybrid

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incompatibility in Arabidopsis is determined by a genetic network. In revision for
Genetics
Dilkes BP, Spielman M, Wiezbauer R, Burkart-Waco D, Watson B, Scott R, and Comai
L 2008 The maternally-expressed WRKY transcription factor TTG2 controls lethality in
interploidy crosses of Arabidopsis. PLoS Biology
Pignatta D, Dilkes BP, Wroblewski T, Michelmore RW, Comai L 2008 Transgeneinduced
gene silencing is not affected by a change in ploidy level. PLoS One
Henry IM, Dilkes BP, Comai L 2007 Genetic basis for dosage sensitivity in Arabidopsis
thaliana. PLoS Genet 3(4): e70 doi:10.1371/journal.pgen.0030070
Henry IM, Dilkes BP, Comai L 2006 Molecular karyotyping and aneuploidy detection in
A. thaliana using quantitative fluorescent PCR. Plant Journal, 48:307-319
Josefsson C, Dilkes B, Comai L 2006 Parent-dependent loss of gene silencing during
interspecies hybridization. Current Biology 16:1322-1328
Coelho CM, Dante RA, Sabelli PA, Sun Y, Dilkes BP, Gordon-Kamm WJ Larkins BA
2005 Cyclin-dependent kinase inhibitors in maize endosperm and their potential role in
endoreduplication. Plant Physiology 138: 2323-36
Henry IM, Dilkes BP, Young K, Watson B, Wu H, Comai L 2005 Aneuploidy and
genetic variation in the Arabidopsis thaliana triploid bridge. Genetics 170: 1979-88
Dilkes BP, Comai L 2004 A differential dosage hypothesis for parental effects in seed
development. Plant Cell 16:3174-80
Hunter BG, Beatty MK, Singletary GW, Hamaker BR, Dilkes BP, Larkins BA, and Jung
R 2002 Maize opaque endosperm mutations create extensive changes in patterns of
gene expression. Plant Cell 14: 14:2591-2612
Gordon-Kamm WJ, Dilkes BP, Lowe KS, Hoerster G, Sun X, Ross M, Church L, Bunde
C, Farrell J, Hill P, Maddock S, Snyder J, Li X, Woo Y, Bidney D and Larkins BA 2002
Stimulation of the cell cycle and maize transformation by disruption of the plant
retinoblastoma pathway. Proc. Natl. Acad. Sci. U S A 99: 11975-11980
Dilkes BP, Dante RD, Coelho C, Larkins BA. 2002 Genetic analyses of
endoreduplication in Zea mays endosperm: evidence of sporophytic and zygotic
maternal control. Genetics 160: 1163-117
Larkins BA, Dilkes BP, Dante RA, Coelho CM, Woo Y, Liu Y 2001 Investigating the
hows and whys of DNA endoreduplication. J. Exp. Bot. 52: 183-92
Sun Y, Dilkes BP, Zhang C, Dante RA, Carneiro NP, Lowe KS, Jung R, Gordon-Kamm
WJ, Larkins BA 1999 Characterization of maize (Zea mays L.) Wee1 and its activity in
developing endosperm. Proc. Natl. Acad. Sci. U S A 96: 4180-5
Choe S, Dilkes BP, Gregory BD, Ross AS, Yuan H, Noguchi T, Fujioka S, Takatsuto S,
Tanaka A, Yoshida S, Tax FE, Feldmann KA 1999 The Arabidopsis dwarf1 mutant is
defective in the conversion of 24-methylenecholesterol to campesterol in
brassinosteroid biosynthesis. Plant Physiol. 119: 897-907

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Choe S, Dilkes BP, Fujioka S, Takatsuto S, Sakurai A, Feldmann KA 1998 The DWF4
gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22alphahydroxylation
steps in brassinosteroid biosynthesis. Plant Cell 10: 231-43
In preparation
Dilkes BP, Josefsson C, and Comai L A shared genetic network controls postzygotic
lethality of both interspecies and interploidy hybrids of Arabidopsis.
Dilkes BP, Henry IM, and Comai L Identification of paternal-effect QTL responsible for
interploidy post-zygotic lethality in Arabidopsis.
Henry IM, Dilkes BP and Comai L Phenotypic consequences of aneuploidy in
Arabidopsis thaliana.
Dilkes BP, Weizbauer R, and Comai L QTL for interploidy F1 lethality and seed size
demonstrate independent genetic regulation of size and survival.
Chang P, Dilkes BP, McMahon S, Comai L, and Nuzdin S. Contribution of cis
regulatory evolution to allele specific expression in Arabidopsis allopoyploids.
Walia H, Josefsson C. Dilkes BP, Kirkbride R, Harada J and Comai L. Dosagedependent
deregulation of an AGAMOUS LIKE GENES cluster contributes to
interspecific incompatibility.
Pignatta D, Dilkes BP, Comai L. Developmental stage sensitizes the two homoeologs
to hybridization in Arabidopsis
RELEVANT TECHNICAL PUBLICATIONS
Gordon-Kamm WJ, Lowe KS, Bailey MA, Gregory CA, Hoerster GJ, Larkins BA, Dilkes
B, Burnett R, Woo YM 2007 Methods of using viral replicase polynucleotides and
polypeptides. U.S. Patent 7,309,813
Gordon-Kamm WJ, Lowe KS, Larkins BA, Dilkes B, Sun Y 2007 Cell cycle nucleic
acids, polypeptides and uses thereof. U.S. Patent 7,256,280
Gordon-Kamm WJ, Lowe KS, Gregory CA, Hoerster GJ, Larkins BA, Dilkes B, Burnett
R 2004 Methods of using viral replicase polynucleotides and polypeptides. U.S. Patent
6,770,800
Sun Y, Dilkes B, Larkins BA, Lowe KS, Gordon-Kamm WJ, Dante RA 2004 Cell cycle
nucleic acids, polypeptides and uses thereof. U.S. Patent 6,777,590
Gordon-Kamm WJ, Lowe KS Bailey MA, Gregory CA, Hoerster GJ, Larkins BA, Dilkes
B, Burnett R, Woo, Y 2002 Methods of using viral replicase polynucleotides and
polypeptides. U.S. Patent 6,452,070
Gordon-Kamm WJ, Lowe KS Bailey MA, Gregory CA, Hoerster GJ, Larkins BA, Dilkes
B, Burnett R, Woo, Y 2001 Methods of using viral replicase polynucleotides and
polypeptides. U.S. Patent 6,284,947

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Dilkes, BP and Larkins BA 1999 "Transgenic plants: where is this technology leading
us" in Biowork II (A. Borem, M.P. Giudice and N.S. Sakiyama, eds.), Federal
University of Vicosa, Vicosa, Brasil, 213-240
Dilkes BP, Feldmann KA 1998 Cloning genes from T-DNA tagged mutants. Methods
Mol. Biol. 82: 339-51
Schulz B, Bennett MJ, Dilkes BP, and Feldmann KA 1995 “T-DNA tagging in
Arabidopsis thaliana: cloning by gene disruption” in Plant Molecular Biology Manual
(Gelvin, S, ed.) Kluwer Academic Publishers, The Netherlands pp. K3:1-17
INVITED TALKS AND PRESENTATIONS
“Genetic networks and the molecular biology of plant speciation” Botanical Society of
America Meeting, Vancouver BC, 2008
“Genomics of speciation in Arabidopsis” International Congress of Brassica Research,
Lilehammer, Norway, 2008
“The genetics of reproductive isolation” Purdue University, West Lafayette, IN, 2008
“Maternal to paternal ploidy balance – effects on seed size” Translational Seed Biology
Meeting, Davis CA, 2007
“The interploidy hybridization barrier is controlled by a genetic network” International
Conference on Polyploidy Heterosis and Epigenetics, Beijing, China, 2007
“Polyploidy-regulated hybridization barriers” Plant Animal Genome Conference, San
Diego, CA, 2007
“Genome dosage effects in sexual hybridization barriers” Genome Center, UC Davis,
Davis, CA, 2006
“Dr. Strangelove, or how I learned to stop worrying and love hybridization barriers”
Washington University, St. Louis, MO, 2006
“Dr. Strangelove, Weapons of Mass Destruction, and the interploidy hybridization
barrier” Purdue University, West Lafayette, IN, 2006
“Dosage effects in interploidy crosses of Arabidopsis” Banbury Conference on Gene
Dosage Polyploidy and Heterosis, Cold Spring Harbor NY, 2005
“Ploidy hybridization barriers: genotype x karyotype interaction” Plant and Animal
Genome Conference, San Diego, CA, 2005
“Epigenetics of Arabidopsis polyploids: gene x genome interactions” Epigenetics
Seminar Series Fred Hutchinson Cancer Research Center, Seattle, WA, 2004
“Cell cycle regulation mediated by maize Rb1 and viral interactors” Maize Genetics
Conference, Lake Geneva, WI, 2001
“Cell cycle control in endoreduplicating maize endosperm” Annual meeting of the

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American Society of Plant Physiology, Baltimore, MD, 2000
“Zea and the art of cell cycle control” Pioneer Hi-Bred Intl., Johnston, IA, 1999
TEACHING EXPERIENCE
Undergraduate level
Laboratory sections of PLS312 (plant genetics; two semesters), BIO181 (introductory
biology for majors; two semesters), and PLS100-H (Honors introductory plant
sciences; one semester) while a graduate teaching assistant at the University of
Arizona.
Guest lecture in Introductory Plant Sciences PLS100 H on “Water Potential,
Transpiration, and Water Use Efficiency” 1997
Co-taught a self defense course within the Experimental College at Oberlin College
Research supervision of three undergraduates at University of Arizona, five at the
University of Washington, and one at the University of California at Davis
Honors Thesis advisor for Sarah Dobrozsi at the University of Washington
Graduate level
Guest instructor for PLB292 a seminar-style mandatory graduate course in The Section
of Plant Biology at the University of California, at Davis 2007
Various lectures in BIO527 a seminar-style plant molecular biology course in the
Department of Biology at the University of Washington 2003-2006
Lecture in BIOC533 a seminar-style minicourse on nuclear structure and organization in
the Department of Biochemistry at the University of Washington Medical School 2005
Research supervision of the Diplom Thesis project of Renate Weizbauer from the
University of Cologne, done in Seattle 2005-2006.
SYNERGISTIC ACTIVITIES and SERVICE
Academic Editor for PLoS One 2006-
Ad-hoc reviewer for Genetics, Genome Research, Molecular Ecology, Molecular Plant,
PLoS One, The Plant Cell, Plant Physiology, The Plant Journal, Plant Molecular
Biology, The New Phytologist, Trends in Plant Science, and Plant Methods.
Ad-hoc reviewer for the National Science Foundation, United States Department of
Agriculture, and University of Washington Royalty Research Fund (2005-2006)
Founding member and Tri-Chair of the University of Washington Post-Doctoral
Association 2005-2006
Frequent panelist in efforts to educate graduate students about selecting high-quality

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postdoctoral training opportunities. Done for the UW Graduate School, UW Biology
Department and HHMI 2004-2006
Co-organizer and participant in the Howard Hughes Medical Institute-sponsored
workshop “Future Faculty Fellows” at the University of Washington 2004
Founding member of Goober Biofuels Racing 2007-