Sandfish hatchery techniques - English version - ACIAR

ANNEX 1: ALGAL CULTURE
Maintenance of algal cultures
Purchase of stock algal cultures
High quality algae to start new cultures are available
from the following organisations:
h CSIRO Marine and Atmospheric Research, GPO
Box 1538, Hobart, Tasmania, 7001 Australia
h Culture Collection of Algae and Protozoa, SAMS
Research Services Ltd, Dunstaffnage Marine
Laboratory, Oban, Argyll PA37 1QA, Scotland
h Plymouth Culture Collection, Marine Biological
Association, Citadel Hill, Plymouth, PL 2PB, U.K.
Maintenance of stock cultures
h Store the stock cultures in Erlenmeyer flasks
(250 ml) in sterile and static conditions, close to
artificial light in an air-conditioned room (20–
24°C).
h Keep two series of stock cultures of each species
to reduce the risks of contamination and complete
loss of algae.
h Swirl the stock cultures by hand every day to
maintain the algae in suspension.
h Sub-culture the stock cultures once a week (or no
longer than 2 weeks).
Figure 54. Stock cultures of algae in Erlenmeyer flasks.
Conditions required for the algal room (volumes of
algae