Sandfish hatchery techniques - English version - ACIAR

Preparation of 20-L carboys, bags and tanks
h Wash with diluted chlorine (10%), rinse and leave
to dry.
h Fill with 1-µm filtered and UV-sterilised
seawater.
h Add chlorine (12.5% active) at a concentration of
0.2 ml/L for a minimum of 4 hours, or overnight
for up to 24 hours without aeration.
h Turn on the aeration for 10–15 minutes to remove
residual chlorine. Add sodium thiosulphate (stock
Figure 56. Algal culture in a 500 L tank.
h
h
solution at 250 g/L) at a rate of 0.2 ml/L for
1 hour to neutralise residual chlorine. Conduct a
chlorine test.
After several minutes, add agricultural fertiliser
such as Aquasol (2.5 g/100 L). For diatom cultures,
also add sodium metasilicate (3.75 g/100 L).
Inoculate with algal cultures.
Sodium metasilicate is added for diatom cultures such as Chaetoceros sp., Navicula sp. and Nitzschia sp.;
diatoms use the silicate for production of an external shell.
ANNEX 1: ALGAL CULTURE
41