Lecture 5: Chapter 4 • Basic molecular genetic ... - Genome Tools

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•Classic EM DNA and RNA txn
•EM nucleolus, RNAP and rRNPs •“old” = heavy and radio isotopes probes •“new” = fluorescent and luminescent probes
Page 1: Lecture 5: Chapter 4 • Basic mole
Page 5 and 6: Nucleotides: Common structure
Page 10 and 11: Why DNA and not RNA, if they are th
Page 12 and 13: •1952 •Franklin and Wilkins had
Page 14 and 15: ‘immediate’ answers… •http:
Page 16 and 17: •dsDNA, complementary and anti-pa
Page 18 and 19: DNA forms: A, B and Z •Right hand
Page 20 and 21: Using the double helix: Proteins
Page 22 and 23: What is DNA? •Native vs denatured
Page 24 and 25: Using the double helix
Page 26 and 27: DNA secondary structures
Page 28 and 29: RNA secondary structures
Page 30 and 31: Nucleic acids have “3-D” struct
Page 32 and 33: Biochemistry of [or manipulation of
Page 34 and 35: Overview of information flow: Centr
Page 36 and 37: Central dogma of molecular biology
Page 38 and 39: ED Belay et al., Emerging Infect Di
Page 40 and 41: Central Dogma of Molecular Biology
Page 42 and 43: •http://sandwalk.blogspot.com/200
Page 44 and 45: Replication: DNA chemistry •Bioch
Page 46 and 47: Transcription (Txn) is RNA from DNA
Page 48: Biochemistry of txn: Model process
Page 52 and 53:
•Refinements thru better tools, b
Page 54 and 55:
A: •Refined Pol II structure •E
Page 56 and 57:
DNA to RNA to protein, what and whe
Page 58 and 59:
Different strategies: Prok vs Euk
Page 60 and 61:
Eukaryotic gene structure in [a lit
Page 62 and 63:
Cell type-specific splicing of fibr
Page 64 and 65:
Record alternative splicing •D. m
Page 66 and 67:
Euk gene: “editing” post-RNA sy
Page 68 and 69:
Euk gene: mature transcript •http
Page 70 and 71:
Prok gene expression regulation: Tr
Page 72 and 73:
Gene expression regulation by • I
Page 75 and 76:
Translation: roles of RNA
Page 77 and 78:
Multiple reading frames •what doe
Page 79 and 80:
-> mRNA is a string of codons --> w
Page 81 and 82:
Importance of codon usage to biotec
Page 83 and 84:
Structure of tRNA determines functi
Page 85 and 86:
Nucleic acids have “3-D” struct
Page 87:
RNA in translation, and proteins…
Page 90 and 91:
•E. coli 70S crystal structure
Page 92 and 93:
•After termination, re-associates
Page 94 and 95:
Translation termination “function
Page 96 and 97:
Recycling model
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Lecture 5: Chapter 4 • Basic molecular genetic ... - Genome Tools

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