Lecture 5: Chapter 4 • Basic molecular genetic ... - Genome Tools

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Central dogma of molecular biology (but...) Prusiner ~82 “prion in disease” Prions (kuru, N. Guinea, early ‘00s; Gadjusek, ‘57; slow virus ‘66) (BSE; UK, Nov86) Also, Genome size paradox ‘05 ‘recent’ gene estimates •Monod ‘54, “What was true for E. coli would be true for the elephant.” -> but, miRNA parallel regulatory system acting on DNA, RNA and protein –Allows structural complexity in humans?
Exception: CWD and deer Oct 5, 2006 EHoover •“Deer spread a brain-destroying disease through saliva” •white-tailed deer at CSU Chronic Wasting Disease Research Facility •saliva from CWD-infected deer to healthy tame from unaffected area •18 month surveillance of tonsil tissue and eventually brain •all deer from saliva exposure as well as single blood transfusion •also, additional work: not just casual contact but contact with environment, eg secretions
Page 1 and 2: Lecture 5: Chapter 4 • Basic mole
Page 3 and 4: •EM nucleolus, RNAP and rRNPs •
Page 5 and 6: Nucleotides: Common structure
Page 10 and 11: Why DNA and not RNA, if they are th
Page 12 and 13: •1952 •Franklin and Wilkins had
Page 14 and 15: ‘immediate’ answers… •http:
Page 16 and 17: •dsDNA, complementary and anti-pa
Page 18 and 19: DNA forms: A, B and Z •Right hand
Page 20 and 21: Using the double helix: Proteins
Page 22 and 23: What is DNA? •Native vs denatured
Page 24 and 25: Using the double helix
Page 26 and 27: DNA secondary structures
Page 28 and 29: RNA secondary structures
Page 30 and 31: Nucleic acids have “3-D” struct
Page 32 and 33: Biochemistry of [or manipulation of
Page 34 and 35: Overview of information flow: Centr
Page 38 and 39: ED Belay et al., Emerging Infect Di
Page 40 and 41: Central Dogma of Molecular Biology
Page 42 and 43: •http://sandwalk.blogspot.com/200
Page 44 and 45: Replication: DNA chemistry •Bioch
Page 46 and 47: Transcription (Txn) is RNA from DNA
Page 48: Biochemistry of txn: Model process
Page 52 and 53: •Refinements thru better tools, b
Page 54 and 55: A: •Refined Pol II structure •E
Page 56 and 57: DNA to RNA to protein, what and whe
Page 58 and 59: Different strategies: Prok vs Euk
Page 60 and 61: Eukaryotic gene structure in [a lit
Page 62 and 63: Cell type-specific splicing of fibr
Page 64 and 65: Record alternative splicing •D. m
Page 66 and 67: Euk gene: “editing” post-RNA sy
Page 68 and 69: Euk gene: mature transcript •http
Page 70 and 71: Prok gene expression regulation: Tr
Page 72 and 73: Gene expression regulation by • I
Page 75 and 76: Translation: roles of RNA
Page 77 and 78: Multiple reading frames •what doe
Page 79 and 80: -> mRNA is a string of codons --> w
Page 81 and 82: Importance of codon usage to biotec
Page 83 and 84: Structure of tRNA determines functi
Page 85 and 86: Nucleic acids have “3-D” struct
Page 87:
RNA in translation, and proteins…
Page 90 and 91:
•E. coli 70S crystal structure
Page 92 and 93:
•After termination, re-associates
Page 94 and 95:
Translation termination “function
Page 96 and 97:
Recycling model
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Lecture 5: Chapter 4 • Basic molecular genetic ... - Genome Tools

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