BD Biopharmaceutical Production

of Select Soytone and TC Yeastolate UF where the total quantity of peptone
added to either BD Cell Medium or IMDM was 3 g/L. In IMDM a significant
increase in antibody yield was achieved when Select Soytone was blended
with a small amount of TC Yeastolate UF compared to the soy or yeast peptone
used alone. However, blending had an inhibitory effect when it was
used in a more optimized base medium like BD Cell Medium. The right combination
of peptones, as well as a consideration of feed strategy, is essential
in order to optimize every portion of the process. Since lot-to-lot consistency
is always a concern when using peptones, evaluations of multiple lots and a
clear understanding of the product specifications is critical.
Purification requirements are greatly reduced when peptones are used as a
replacement for serum or any of its derived proteins. The gel in Figure 9
shows the differences in unpurified supernatants of hybridoma cells grown
Figure 7
450
400
350
300
250
200
150
100
50
0
Figure 8
2000
1800
1600
1400
1200
1000
800
600
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200
0
Antibody Production of Select Soytone and TC Yeastolate UF Blends in IMDM
MAR 18.5 ATCC TIB-216
70% Soy/30% Yeast
50% Soy/50% Yeast
30% Soy/50% Yeast
100% Soy
100% Yeast
Antibody Production of Select Soytone and TC Yeastolate UF Blends in
BD Cell Medium
70% Soy/30% Yeast
50% Soy/50% Yeast
30% Soy/70% Yeast
100% Soy
100% Yeast
MAR 18.5 ATCC TIB-216
BIONUTRIENT TECHNICAL MANUAL • SECOND EDITION
Figure 6
Antibody Production in Different Basal Media
46/4 ATCC HB67
350
Select Soytone
TC Yeastolate
300
No Peptone
250
200
150
100
50
0
IMDM
BD Cell Medium
9