BD Biopharmaceutical Production

Molecular Weights
Select Soytone
Percentage Area
60
50
40
30
20
10
0
20000
short chain or free amino acids, others benefit from longer chain amino acids. While
the typical analysis profiles for each peptone in this manual can help direct the end-user
to the correct peptone match, it is recommended that end-users supplement the typical
analytical information with evaluations in their own individual growth models.
BD offers two ultrafiltered soy peptone products suitable for cell culture purposes;
Difco Springer DS100 Soy Peptone UF is an animal-free product processed in
dedicated animal-free equipment. This product is intended for use as a nutritional
supplement in cell culture, laboratory media and microbial fermentation. Low endotoxin
levels make Difco Springer DS100 Soy Peptone UF an ideal substitute for serum. It may
also be used in highly concentrated feed solutions for bacterial fermentation applications.
Cell viability results yielded greater than or equal to 85% viability with Mink Lung cells
for murine and feline sarcoma virus assay (ML ATCC CCL-64) and with Green Monkey
Kidney cells for verotoxin detection (VERO ATCC CCL-81). DS100 Soy Peptone UF has an
endotoxin level of less than 300 EU/g.
Growth Curve
Average OD at 600nm
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
1% Select Soytone in 1.13% M9 Minimal Salts + 0.4% Glucose, BioScreen C
Bacillus subtilis ATCC 6633
Escherichia coli ATCC 47014
Staphylococcus aureus ATCC 25923
Saccharomyces cerevisiae ATCC 287
Enterococcus faecalis ATCC 29212
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 41 43 45 47 49
Time in Hours
Growth Curve
Average OD at 600nm
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
1% Bacto Soytone in 1.13% M9 Minimal Salts + 0.4% Glucose, BioScreen C
Bacillus subtilis ATCC 6633
Escherichia coli ATCC 47014
Staphylococcus aureus ATCC 25923
Saccharomyces cerevisiae ATCC 287
Enterococcus faecalis ATCC 29212
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 41 43 45 47 49
Time in Hours
46 BD BIOPHARMACEUTICAL PRODUCTION