Infectious Pancreatic Necrosis Virus (IPNV) - Fróðskaparsetur Føroya

3 Methods
We examined one salmon producing company for Listeria monocytogenes. Samples were
taken at the harvesting plant and at the processing plant. Samples were taken of raw material,
seawater, production equipment and manufactured goods. Samples of the production
equipment were taken after cleaning and disinfection, and at least 2 hours after production.
Figure 1 shows how the processing is designed.
3.1 Samples
All samples taken inside the harvesting plant and processing plant were taken as swab
samples. The water samples were taken with 9 litres sterile cans. Fish chops were sampled at
the processing plant and examined for Listeria monocytogenes.
3.1.1 Materials
Swab samples were taken with bio-spo TM (Environmental Sampling Products, Solar
Biological Inc.). After using the swab cloth, it was returned to the sterile bag that it came
from. When samples were taken of the cleaned equipment, the swabs were soaked in
neutralizing buffer (Difco 8902823y5).
Gloves were used when the samples were taken. The samples were stored at 5 °C, until
further processing in the laboratory.
3.1.2 Samples: Production Equipment, Raw Material, Manufactured Goods and
Seawater Samples
The samples of the clean production equipment were taken one day before the factory started
production. Samples taken during production were taken at least 2 hours after production
started. Swab samples were taken of fish after stunning in the harvesting plant and of finished
produced fish in the processing plant. The 9 litres seawater samples were taken of surface
water over a period of 6 months, from October 2010 to March 2011.
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